Alzheimer's disease (AD) is characterized by cognitive and functional deterioration, with pathological features such as amyloid-beta (Aβ) aggregates in the extracellular spaces of parenchymal neurons and intracellular neurofibrillary tangles formed by the hyperphosphorylation of tau protein. Despite a thorough investigation, current treatments targeting the reduction of Aβ production, promotion of its clearance, and inhibition of tau protein phosphorylation and aggregation have not met clinical expectations, posing a substantial obstacle in the development of drugs for AD. Recently, artificial intelligence (AI), computational biology (CB), and systems biology (SB) have emerged as promising methodologies in AD research. Their capacity to analyze extensive and varied datasets facilitates the identification of intricate patterns, thereby enriching our comprehension of AD pathology. This paper provides a comprehensive examination of the utilization of AI, CB, and SB in the diagnosis of AD, including the use of imaging omics for early detection, drug discovery methods such as lecanemab, and complementary therapies like phototherapy. This review offers novel perspectives and potential avenues for further research in the realm of translational AD studies.

The immune checkpoint blockade therapy has profoundly revolutionized the field of cancer immunotherapy. However, despite great promise for a variety of cancers, the efficacy of immune checkpoint inhibitors is still low in colorectal cancer (CRC). This is mainly due to the immunosuppressive feature of the tumor microenvironment (TME). Emerging evidence reveals that certain chemotherapeutic drugs induce immunogenic cell death (ICD), demonstrating great potential for remodeling the immunosuppressive TME. In this study, the potential of ginsenoside Rg3 (Rg3) as an ICD inducer against CRC cells was confirmed using in vitro and in vivo experimental approaches. The ICD efficacy of Rg3 could be significantly enhanced by quercetin (QTN) that elicited reactive oxygen species (ROS). To ameliorate in vivo delivery barriers associated with chemotherapeutic drugs, a folate (FA)-targeted polyethylene glycol (PEG)-modified amphiphilic cyclodextrin nanoparticle (NP) was developed for co-encapsulation of Rg3 and QTN. The resultant nanoformulation (CD-PEG-FA.Rg3.QTN) significantly prolonged blood circulation and enhanced tumor targeting in an orthotopic CRC mouse model, resulting in the conversion of immunosuppressive TME. Furthermore, the CD-PEG-FA.Rg3.QTN achieved significantly longer survival of animals in combination with Anti-PD-L1. The study provides a promising strategy for the treatment of CRC.

Objective:To construct a standard index system for the outpatient job competency of midwife, and to clarify the midwives' abilities when providing outpatient services, so as to improve the midwives' core competencies and service satisfaction.Methods:A research group was established in December 2018. On the basis of literature review and survey of midwives' outpatient needs in the early stage, referring to expert opinions, and taking job competency theory as the theoretical framework, the standard index system for the outpatient job competency of midwife were initially constructed. From December 2018 to March 2019, convenience sampling was used to select 24 experts from different ClassⅢ Grade A hospitals of Beijing, and the Delphi method was used to conduct 2 rounds of expert consultations.Results:Among 2 rounds of expert consultations, the effective recovery rates of the questionnaires were all 100.0% (24/24) , the positive coefficient of experts was 100.0%, and the coefficient of authority of experts was 0.786. The Kendall coordination coefficients of the 2 rounds of correspondence were 0.030 and 0.400, respectively ( P<0.05) . The final construction of standard index system for the outpatient job competency of midwife included 4 first-level indicators, 9 second-level indicators and 24 third-level indicators of knowledge and skills, professional quality, health education ability, and ability to improve the quality of professional services. Conclusions:The standard index system for the outpatient job competency of midwife is scientific and reasonable, comprehensive in content, and strong in specialty, which can provide a reference for the training and assessment of midwife.

Objective:To explore the damage of panretinal photocoagulation (PRP) to the subbasal nerve plexus (SNP) and its related mechanisms by comparing SNP changes in wide-field mosaic between before and after PRP treatment in diabetic patients.Methods:A randomized controlled study was conducted.Fifty-seven patients (114 eyes) with type 2 diabetes mellitus and binocular diabetic retinopathy (DR) stage IV to receive PRP treatment in Shanxi Eye Hospital from April to November 2019 were enrolled.The subjects were randomly divided into horizontal-vertical laser group and vertical-horizontal laser group according to a random number table.Twenty-nine eyes from 29 patients were assigned to the horizontal-vertical laser group with the photocoagulation sequence of temporal-nasal-inferior-superior.Twenty-eight eyes from 28 patients were assigned to the vertical-horizontal laser group with the photocoagulation sequence of inferior-superior-temporal-nasal.The severer eyes of each subject were chosen as the treatment eye and the contralateral eyes were chosen as the control eye.Corneal confocal laser scanning microscopy (CCM) was performed before PRP treatment, 1 week after each photocoagulation, and 1 month after the completion of PRP treatment to collect images of the SNP over an area of 2-3 mm around the whorl-like pattern.Captured images at each time were merged into one image by using the Photoshop CC 2017 image processing software, and then the nerve fiber length (NFL) of whorl-like pattern was measured by Neuron J image analysis software.McGill pain questionnaire was used to investigate the pain of patients after each photocoagulation.The NFL changes of SNP at different time points were compared between different eyes and different photocoagulation sequence groups.The study protocol adhered to the Declaration of Helsinki and was approved by an Ethics Committee of Shanxi Eye Hospital (No.201804b). Written informed consent was obtained from each patient prior to entering the study cohort.Results:After PRP treatment, there were different degrees of neural structure loss of SNP nerve fibers in 11 treatment eyes, but there was no significant change in SNP nerve fibers in the control eyes.There were significant differences in NFL between the treatment eyes and the control eyes at various time points ( Feyes=2.020, P=0.039; Ftime=4.062, P=0.001). In the horizontal-vertical laser group, different degrees of neural structure loss on the photocoagulation side were found in SNP nerve fibers after the first and second photocoagulation.In the vertical-horizontal laser group, different degrees of neural structure loss on the photocoagulation side were found in SNP nerve fibers after the third and fourth photocoagulation.There was no significant difference in NFL of treatment eyes between the two groups ( Fgroup=0.099, P=0.754), but there was a significant difference in NFL at various time points before and after treatment ( Ftime=5.231, P<0.001). There were 9 (9/57) patients who complained of pain after PRP, which occurred at the first time of photocoagulation in 7 of them. Conclusions:SNP damage may occur after PRP in patients with DR, and SNP is prone to be damaged on the photocoagulation side when performing horizontal photocoagulation.

Objective To analyze the changes and gender differences of organ coefficients, intestinal length and body size of SJ5-SPF chickens at different weeks of age,and to provide experimental data for the sex selection of laboratory chickens. Methods The body weight,the weight of 15 main organs,and length of 5 main segments of intestine and the 6 main parameters of body size of SJ5-SPF chickens at the age of 4,20,25 and 40 weeks were measured,respectively,and the organ coefficients were calculated. In addition, the gender differences in body weights, organ coefficients, intestinal lengths and body sizes between the female and male SJ5-SPF chickens at the four different weeks of age were compared, respectively. Results There were significant differences in body weights between the male and female SJ5-SPF chickens at each of the four different weeks of age(P < 0.01). The organ coefficients varied between females and males to different extents at different weeks of age. As for the intestinal length,there were no significant differences in the length of jejunum and ileum as well as rectum between the male and female chickens, while the length of duodenum, left cecum and right cecum showed gender differences at some of the four different weeks of age. During the determination of body size, there were gender differences in body length,shank length,pelvic width,chest depth and chest width at two or three of the four different weeks of age. Conclusions The organ coefficients, intestinal length and body size of SJ5-SPF chickens are affected by both their age and gender.

Objective To study the effect of age and sex on physiological and biochemical indexes of SJ5-SPF chickens at the age of different weeks. Methods Blood biochemical indexes of SJ5-SPF chickens at the age of different weeks were measured using an automatic blood analyzer, including 19 items: ALT, ALP, GGT, AST, LDH, TP, GLB, ALB, TB, DB, IBIL, GLU, TG, TCH, BUN, CRE, K+, Na+, and Ca2+. At the same time, the body temperature (T), respiratory frequency (R), heart rate (HR), diastolic pressure (DBP) and systolic pressure (SBP) of the SJ5-SPF chickens were measured using a RM6240C multi-channel physiological signal acquisition and processing system. Results (1) Among the physiological parameters, the body temperature, respiratory rate, heart rate, diastolic blood pressure and systolic blood pressure showed significant differences between the 4-week old chickens and the chickens at ages of 20, 25, and 25 weeks (P ＜ 0. 05), but there were significant differences between the males and females at the same weeks of age except body temperature. The diastolic blood pressure was only significantly different in the 40-week old male and female chickens (P ＜ 0. 01). The heart rates showed significant differences between the male and female chickens at 4, 20 and 25 weeks of age (P ＜ 0. 01). The respiratory rate and systolic blood pressure in the 4-week old male and female chickens were significantly different (P ＜ 0. 05). (2) The 19 blood biochemical indexes showed significant differences between the male and female chickens: the GGT, ALT, AST, TB, DB, IBIL, GLU, BUN and Na+at an week-age (P＜ 0. 05), the LDH, TP, GLB, ALB and CRE at two different week-ages (P＜ 0. 05), the ALP, TG and Ca2+at three different week-ages (P＜ 0. 05), and the TCH and K+at four different week-ages (P＜ 0. 05). Conclusions The above results can provide a scientific basis for diagnosis of SJ5-SPF chicken disease, animal quarantine and other related research.

Objective To investigate the influencing factors of cognitive dysfunction in elderly patients with hypertension.Methods From October 2015 to October 2016,one hundred and fifty-one elderly hypertensive patients treated in the Affiliated Hospital of North China University of Science and Technologywere selected as the observation group,and 151 healthy subjects in the same period were enrolled in the control group.The blood pressure measurement and cognitive function evaluation were given to analyze the influencing factors of elderly hypertensive patients with cognitive dysfunction.Results Diastolic pressure ((98.5 ±4.2) mmHg),systolic pressure ((157.6 ± 8.9) mmHg),pulse pressure ((59.1 ± 4.3) mmHg) and cognitive impairment rate(27.2％) in observation group were higher than those in the control group((82.7±3.6 mmHg,(122.4± 6.8) mmHg,(39.7 ± 2.5) mmHg,0.0％),the differences were statistically significant (t =4.951,5.868,8.128,P＜ 0.05).The language fluency ((2.5 ± 0.2) points),memory ability ((2.7 ± 0.4) points),visual spatial executive ability ((2.9±0.2) points),naming ability ((2.6±0.1 points),abstract generalization ability ((1.7±0.2) points),orientation ability ((5.0 ± 0.4) points),attention and calculation ability ((5.1 ±0.3) points),total score ((22.5±0.8) points)in observation group were lower than those in the control group ((2.8±0.1) points,(4.8 ± 0.2) points,(4.7 ± 0.3) points,(2.8 ± 0.2) points,(1.9 ± 0.1) points,(5.7 ±0.1) points,(5.7±0.2) points,(28.4±0.6) points) (t=4.202,10.778,9.206,3.769,4.173,4.405,4.278,5.727,P＜0.05).Diastolic pressure ((103.7 ± 2.5)mmHg),systolic pressure ((164.8 ±4.1) mmHg),pulse pressure((64.5± 1.6) mmHg)in patients with cognitive dysfunction were higher than those in patients without cognitive dysfunction ((92.6±2.8) mmHg,(145.7±3.9) mmHg,(46.3± 1.5) mmHg) (t =4.195,4.310,6.913,P＜0.05).Age distribution (≤65 years old in 9 cases,＞65 years old in 32 cases),BMI index distribution (≤24 kg/m2 in 11 cases,＞24 kg/m2 in 30 cases) in patients without cognitive dysfunction were better than those in patients with cognitive dysfunction ((≤ 65 yeas old in 64 cases,＞ 65 years old in 46 cases),(≤ 24 kg/m2 in 68 cases,＞ 24 kg/m2 in 42 cases)).The differences were statistically significant (x2 =15.700,14.657,P ＜ 0.05).Multiple linear regression analysis showed that cognitive function in elderly hypertensive patients was related to age,BMI index,diastolic blood pressure and pulse pressure (OR (95％CI):4.265 (2.038 ～ 8.927),3.991 (1.956～8.142),3.294 (1.532 ～ 7.084),3.521 (1.640 ～ 7.561),5 (2.375 ～10.528);P=0.000).Condusion Age,BMI index,diastolic blood pressure,systolic blood pressure,pulse pressure are influencing factors in elderly hypertension patients with cognitive dysfunction.

Objective To investigate the relationship between mild cognitive impairment and quality of life in hemodialysis patients with diabetic nephropathy.Methods One hundred and twenty-five hemodialysis patients with diabetic nephropathy were selected in Affiliated Hospital of North China University of Science and Technology from December 2014 to December 2016,and were divided into two groups according to mild cognitive impairment,including 73 patients without mild cognitive impairment as control group,and 52 patients with mild cognitive impairment as observation group.Patients received Montreal cognitive assessment scale and kidney disease quality of life scale.Patients received clinical index detection.Results Montreal cognitive assessment scale(visual space and execution(3.0±0.7) points,naming(2.4±0.3) points,attention(4.3±0.7) points,language(2.1±0.4) points,abstraction(1.1±0.2) points,delayed recall(1.6±0.3) points,orientation(5.4±0.5) points) score,kidney disease quality of life scale(physical(70.3±3.5) points,physical constraints due to work and life(14.6±2.8) points,pain(56.4±7.1) points,general health(36.2±3.4) points,emotional status(51.3±2.6) points,social function(52.7±3.4) points,mental status(43.2±3.6) points,emotional constraints due to work and life(41.3±2.8) points,symptoms(73.6±2.1) points,work status(20.3±1.6) points,cognitive function(72.7±1.8) points,nephropathy effect(49.1±3.2) points,sexual function(53.2±4.0) points,burden of nephropathy(21.3±2.6) points,sleep quality(70.1±1.5) points,social quality(43.3±1.9) points,social support(71.2±2.3) points) score in observation group were lower than control group((4.4±0.8) points,(3.0±0.0) points,(5.4±0.5) points,(2.7±0.3) points,(1.8±0.4) points,(3.4±0.6) points,(6.0±0.0) points),((78.6±4.1) points,(25.7±3.6) points,(69.8±4.2) points,(41.0±2.7) points,(58.5±3.0) points,(61.2±3.8) points,(53.4±2.8) points,(46.5±2.4) points,(82.9±3.2) points,(24.2±2.7) points,(81.6±2.3) points,(65.3±4.0) points,(62.8±3.9) points,(35.7±2.8) points,(79.3±2.0) points,(59.7±2.4) points,(82.4±2.6) points)(t=7.667,5.502,5.558,5.857,9.364,14.250,4.112,4.038,10.603,5.321,4.326,4.403,4.613,5.361,4.262,4.315,4.923,4.224,6.265,4.804,6.712,4.316,6.787,4.573,all P<0.05).Conclusion Hemodialysis patients with diabetic nephropathy has mild cognitive impairment,while quality of life is low.

Objective To investigate the current status and influencing factors of job burnout and self efficacy of midwives in Beijing, and to explore the correlation between job burnout and self efficacy in midwives. Methods We selected 335 midwives in wards of obstetrics department from 20 hospitals ( 13 general hospitals+ 7 women-children special hospitals ) in Beijing as respondents by convenient sampling. And then, we investigated the job burnout, self efficacy and the influencing factors. Results The total average score of job burnout of 335 midwives was (70.0±16.1). There were 5 (1.5%) with zero burnout, 73 (21.8%) with mild burnout, 178 (53.1%) with moderate burnout, 79 (23.6%) with severe burnout among 335 midwives. The total average score of self efficacy of 335 midwives was (26.4±5.9). The major influencing factors of job burnout and self efficacy were the age, working hours in clinic, working hours in delivery room (P<0.05). The regression showed that there was significant correlation between each dimension of job burnout and self efficacy. Conclusions The degree of job burnout is high and the self efficacy is the medium level in the midwife. The effective measures should be taken to enhance the midwives′self efficacy and improve their job burnout.

Objective To describe the temperature variation within 24 h of the children with congenital heart disease after open heart surgery , the incidence of fever and the relation between fever and clinical outcome, aiming to provide basis for clinical nursing .Methods The convenience sampling method was used to select 200 cases of children with congenital heart disease after open heart operation , collecting body temperature within postoperative 24 hours and clinical outcomes .Results The body temperature of children reached the peak after three hours of open heart operation , then it began to slow down .The fever rate of early postoperative period (within 24 h) was 63.5%.The demographic data of fever group (≥38.0 ℃) and no fever group (<38.0℃) had no significant difference(P>0.05), except for the aspect of clinical diagnosis (χ2 =10.641, P=0.001).The median of ICU stay length, the ventilation time and the total length of hospital stay in fever group and no fever group were 68.50 h and 46.00 h, 20.00 h and 16.00 h, 16.00 d and 12.00 d, which were significantly different (Z =-1.971,-1.998,-3.700, respectively;P<0.05).Conclusions The body temperatures of children with congenital heart disease after open heart operation reach the peak after three hours . More than half of postoperative children will appear postoperative fever , which could affect the clinical outcome .