Objective To investigate the effects of gut microbiota regulation combined with exercise rehabilitation on non-motor symptoms and neurological function in Parkinson's disease patients.Methods A total of 154 Parkinson's disease patients admitted to our hospital from January 2022 to January 2024 were selected as the subjects of the study.Using a random number table,these 154 patients were evenly divided into a control group and a treatment group,with 77 patients in each group.Both groups received standard treatments,but the control group also underwent exercise rehabilitation therapy,while the treatment group received probiotic supplementation and exercise rehabilitation therapy.The effectiveness of the two groups was then compared.Results Following the 12 weeks,24 weeks therapeutic regimen,The treatment group showed significantly better outcomes(P<0.05).Clinically meaningful reductions were observed in Hoehn-Yahr staging,alongside decreased scores on standardized instruments assessing psychiatric symptoms HAMA,HAMD,SCOPA-AUT,UPDRS Ⅰ-IV and PDSS(P<0.05).Concurrently,the study group exhibited enhanced MMSE(P<0.05).Fecal microbiome analyses revealed a favorable ecological shift characterized by increased colonization of beneficial genera Bifidobacterium and Lactobacillus,with concomitant suppression of pathobionts Enterococcus and Enterobacteriaceae.Gait analysis revealed increased step length,speed,and frequency in the treatment group(P<0.05).Conclusion Patients with Parkinson's disease who received probiotics combined with exercise rehabilitation treatment could effectively improve non-motor symptoms and neurological function,while promoting the balance of intestinal flora,and reduce clinical symptoms.

Objective To investigate the effects of gut microbiota regulation combined with exercise rehabilitation on non-motor symptoms and neurological function in Parkinson's disease patients.Methods A total of 154 Parkinson's disease patients admitted to our hospital from January 2022 to January 2024 were selected as the subjects of the study.Using a random number table,these 154 patients were evenly divided into a control group and a treatment group,with 77 patients in each group.Both groups received standard treatments,but the control group also underwent exercise rehabilitation therapy,while the treatment group received probiotic supplementation and exercise rehabilitation therapy.The effectiveness of the two groups was then compared.Results Following the 12 weeks,24 weeks therapeutic regimen,The treatment group showed significantly better outcomes(P<0.05).Clinically meaningful reductions were observed in Hoehn-Yahr staging,alongside decreased scores on standardized instruments assessing psychiatric symptoms HAMA,HAMD,SCOPA-AUT,UPDRS Ⅰ-IV and PDSS(P<0.05).Concurrently,the study group exhibited enhanced MMSE(P<0.05).Fecal microbiome analyses revealed a favorable ecological shift characterized by increased colonization of beneficial genera Bifidobacterium and Lactobacillus,with concomitant suppression of pathobionts Enterococcus and Enterobacteriaceae.Gait analysis revealed increased step length,speed,and frequency in the treatment group(P<0.05).Conclusion Patients with Parkinson's disease who received probiotics combined with exercise rehabilitation treatment could effectively improve non-motor symptoms and neurological function,while promoting the balance of intestinal flora,and reduce clinical symptoms.

Objective:To explore the effect on cardiopulmonary function of early rehabilitation after lung transplantation.Methods:Forty patients undergoing lung transplantation were randomly divided into an observation group and a control group, each of 20. Both groups received routine rehabilitation treatment, psychological counseling and health education about rehabilitation, medications and nutrition. The observation group was additionally given systematic early lung rehabilitation treatment as soon as their vital signs were stable after the operation. It included body position transfers, aerobic training, resistance training and breathing training 3 to 5 times a week for 4 weeks supervised by physical therapists. The control group were informed about methods of aerobic training, respiratory training and airway clearance but carried them out by themselves. The percentage of forced expiratory volume (FVC%), FVC in the first second (FEV1%, ) and FEV1%/ FVC% of the two groups were evaluated using a bedside lung function instrument before the lung transplantation and after 4 weeks of treatment. The six-minute walking distances (6MWDs) of the two groups were compared and their facility in the activities of daily living was evaluated using the modified Barthel index (MBI).Results:Before treatment, there was no significant difference in the average FEV1%, FVC%, FEV1%/FVC%, 6MWD or MBI ratings between the two groups. After the 4 weeks of treatment, both groups showed significant improvement in all of those measurements, on average. The observation group′s averages were, however, significantly better than those of the control group.Conclusion:Early rehabilitation after lung transplantation helps to improve cardiopulmonary function and promote a better quality of life.

Objective To investigate and analyze the capabilities of Plasmodium microscopic examinations in medical and health organizations in Dongxihu District,Wuhan City,so as to provide evidences for achieving the standard of malaria elimina?tion and malaria control in the future. Methods The basic information of the laboratorians and the hardware facilities in 15 mi?croscopic examination stations of Plasmodium were collected and analyzed from 2010 to 2015. Meanwhile,the quality of mak?ing,dyeing,cleanliness of all the negatives blood smears were evaluated,and all the positive smears were re?checked in the dis?trict during the period above mentioned. Results Totally,there were 28 laboratorians and 9 qualified microscopes in 15 micros?copy stations in Dongxihu District. The age of the laboratorians in the first?level and third?level organizations was mainly from 40 to 50 years old,but the age of those in the second?level organizations was from 20 to 30 years old. The years for microscopic ex?amination working of most of the laboratorians in the first?level and third?level organizations were more than 15 years,while the working years of those in the second?level organizations were mainly less than 10 years. The education level of the laboratorians in the first?level organization was mainly(50.00%)junior college,while the education levels of those in the second and third or?ganizations were mainly bachelor. From 2010 to 2015,8 561 blood smears were produced and 539 of them were reviewed,the co?incidence rates of review were all 100%,and the average qualified rates of making,dyeing,and cleanliness of the negative blood smears were 82.08%,79.92%and 83.33%,respectively. All the rates mentioned above in 2015 were higher than those in 2010(χ2=26.45,16.85,13.93,all P<0.01). Conclusion The capability of microscopic examinations of Plasmodium has improved obviously in Dongxihu District,but the human resources construction and hardware input in this district should be im?proved,meanwhile,the check on the quality control of microscopic examinations of Plasmodium should be continued,so as to guarantee the sustainable and healthy development of malaria control work in this district.

Objective To evaluate proximal gastric function in patients with functional dyspepsia (FD) met Rome Ⅲ criteria and its subtypes.Methods Thirty FD patients were enrolled,including 15 patients with postprandial distress syndrome (PDS) and 15 patients with epigastric pain syndrome (EPS).A total of 30 healthy volunteers were recruited as control.All the subjects received barostat examination.Minimal distending pressure (MDP),pressure and volume of thresholds for first sensation and maximal tolerance for gastric sensitivity were recorded.When the pressure level was MDP+2 mmHg (1 mmHg=0.133 kPa),average volume at 30 minutes before meal,averaged volume at 60 minutes after meal and maximum accommodation volume after meal were recorded,and receptive diastolic volume was calculated.The gastric hypersensitivity and impaired accommodation were observed.Student's t test or x2 test was performed for statistical analysis.Results MDP,pressure of first-sensation,maximum sensation pressure,volume of first sensation and maximal sensation volume of FD group were (6.17 ±1.95) mmHg,(8.44±2.01) mmHg,(14.62±3.72) mmHg,(123.59±53.26) mL and (451.26±140.44) mL,respectively; which were lower than those of healthy control group ((9.27±1.99) mmHg,(12.04±2.66) mmHg,(19.74±4.18) mmHg,(168.41±73.06) mL and (556.89±124.07) mL),and the differences were statistically significant (t=-6.080,-5.900,-5.011,-2.723 and-2.995,all P＜0.01).The averaged volume at 30 minutes before meal and 60 minutes after meal was (212.19±120.82) mL and (333.97±121.86) mL while those of healthy control group was (191.69±66.19) mL and (385.58±83.05) mL,and there were no significant differences between the two groups (both P＞0.05).The maximum postprandial accommodation volume and receptive diastolic volume of FD group were (405.10±111.29) mL and (190.16±97.22) mL,which were lower than those of healthy control group ((461.10±87.60) mL and (262.83±78.39) mL),and the differences were statistically significant (t=-2.599 and-3.187,both P＜0.05).The maximum postprandial accommodation volume of FD patients was at 15 to 20 minutes after meal and in healthy control it was at five to ten minutes after meal.Among the 30 FD patients,12 patients (40 ％) had gastric hypersensitivity,including eight PDS patients and four EPS patients,and there was no significant difference in the ratio between two groups (P＞0.05) ;nine patients (30 ％) had impaired accommodation,including five PDS patients and four EPS patients,and there was no significant difference in the ratio between two groups (P＞ 0.05).Conclusions Gastric hypersensitivity and impaired accommodation often appear in FD patients,and there is no significant difference between PDS and EPS subtypes.FD patients with different pathophysiologic mechanisms cannot be efficiently distinguished according to Rome Ⅲ criteria.

Objective To investigate the effect of hyperbaric oxygen (HBO) on cognitive function in rats following traumatic brain injury.Methods Thirty-six male Sprague-Dawley rats were randomly divided into the HBO group,normobaric air therapy group and the sham surgical group,each consisting of 12 animals.Traumatic brain injury model was established by using Feeney's free falling in the animals of the HBO group and the normobaric air therapy group.The HBO group received HBO therapy every day and the normobaric air therapy group was left there in normal atmospheric air.The animals in the sham surgical group just exposed dura mater,but without free fall impact.Cognitive function was assessed by using Morris water maze.Dynamic changes in N-acetylaspartate/creatine (NAA/Cr) levels in hippocampus and NAA/choline (NAA/Ch) levels were closely observed with H-MRS following treatment of traumatic brain injury with HBO,and real-time PCR was used to evaluate changes in the levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA in the ipsilateral hippocampus.Results Seven days after HBO therapy,escape latency decreased [(35.1 ± 2.8) s] and number of times crossing the platform increased for the animals in the HBO group.Statistical significance could be noted,when they were compared with those of the normobaric air therapy group[(59.4 ±5.0) s;2.44 ±0.45] (P ＜0.05).NAA/Cr levels in the HBO group were significantly increased (1.18 ± 0.11) 7 days after HBO therapy,and statistical significance could also be seen,as compared with those of the normobaric air therapy group(0.85 ± 0.03) (P ＜ 0.05).Compared with those of the normobaric air therapy group (8 h:0.79 ± 0.07 ; 7 d:0.86 ± 0.08),NAA/Ch levels in the ipsilateral hippocampus of the HBO group (8 h:1.14 ± 0.10; 7 d:1.33 ± 0.17) displayed statistical significance at h 8 and d 7 (P＜0.05).The levels of NGF(3 d:1.27 ±0.03; 7 d:1.31 ±0.08)and BDNF mRNA for the HBO group (3 d:2.82 ±0.20; 7 d:1.07 ±0.08)and BDNF mRNA(3 d:2.82±0.20; 7 d:1.07 ±0.19)were increased at d 3 and d 7,as compared with those of the normobaric air therapy group(NGF 3 d:0.66 ±0.10,7 d:0.73 ±0.07); (BDNF 3 d:1.51 ±0.11; 7 d:0.65±0.09),with statistical significances (P ＜ 0.05).Conclusions HBO therapy could promote cognitive function in rats with traumatic brain injury,the possible mechanism might be associated with the increases in NAA/Cr and NAA/Ch levels and the levels of NGF and BDNF mRNA in hippocampus.

Objective To investigate the effect of hyperbaric oxygen (HBO) on cognitive function in rats following traumatic brain injury.Methods Thirty-six male Sprague-Dawley rats were randomly divided into the HBO group,normobaric air therapy group and the sham surgical group,each consisting of 12 animals.Traumatic brain injury model was established by using Feeney's free falling in the animals of the HBO group and the normobaric air therapy group.The HBO group received HBO therapy every day and the normobaric air therapy group was left there in normal atmospheric air.The animals in the sham surgical group just exposed dura mater,but without free fall impact.Cognitive function was assessed by using Morris water maze.Dynamic changes in N-acetylaspartate/creatine (NAA/Cr) levels in hippocampus and NAA/choline (NAA/Ch) levels were closely observed with H-MRS following treatment of traumatic brain injury with HBO,and real-time PCR was used to evaluate changes in the levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA in the ipsilateral hippocampus.Results Seven days after HBO therapy,escape latency decreased [(35.1 ± 2.8) s] and number of times crossing the platform increased for the animals in the HBO group.Statistical significance could be noted,when they were compared with those of the normobaric air therapy group[(59.4 ±5.0) s;2.44 ±0.45] (P ＜0.05).NAA/Cr levels in the HBO group were significantly increased (1.18 ± 0.11) 7 days after HBO therapy,and statistical significance could also be seen,as compared with those of the normobaric air therapy group(0.85 ± 0.03) (P ＜ 0.05).Compared with those of the normobaric air therapy group (8 h:0.79 ± 0.07 ; 7 d:0.86 ± 0.08),NAA/Ch levels in the ipsilateral hippocampus of the HBO group (8 h:1.14 ± 0.10; 7 d:1.33 ± 0.17) displayed statistical significance at h 8 and d 7 (P＜0.05).The levels of NGF(3 d:1.27 ±0.03; 7 d:1.31 ±0.08)and BDNF mRNA for the HBO group (3 d:2.82 ±0.20; 7 d:1.07 ±0.08)and BDNF mRNA(3 d:2.82±0.20; 7 d:1.07 ±0.19)were increased at d 3 and d 7,as compared with those of the normobaric air therapy group(NGF 3 d:0.66 ±0.10,7 d:0.73 ±0.07); (BDNF 3 d:1.51 ±0.11; 7 d:0.65±0.09),with statistical significances (P ＜ 0.05).Conclusions HBO therapy could promote cognitive function in rats with traumatic brain injury,the possible mechanism might be associated with the increases in NAA/Cr and NAA/Ch levels and the levels of NGF and BDNF mRNA in hippocampus.