ObjectiveTo reveal the correlation of Rehmannia glutinosa-soil feedback process with the formation of its continuous cropping obstacles through the identification of the root exudates of R. glutinosa and analysis of the specific rhizomicrobes recruited by the root exudate. MethodThe root exudates of R. glutinosa seedlings germinated under sterilized condition and those enriched in the rhizosphere of R. glutinosa cultivated in the field were collected and analyzed using the ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF-MS）. The highly abundant compounds identified in the root exudates were added into blank soil， and the soil microbial community was profiled using Illumina Miseq sequencing. The bacterial and fungal functions were predicted by PICRUSt and FUNGuild， respectively. ResultThe identification results showed that seven phenylethanoid glycosides were found in R. glutinosa root exudates， and acteoside possessed the highest abundance. In the soil enriched with acteoside， the bacterial genera such as Agromyces， Pseudomonas， Lysobacter， Sphingobium， Pseudoxanthomonas and Sphingomonas were enriched. For the fungi， the genera Neocosmospora， Plectosphaerella and Dactylonectria， and the species such as Neocosmospora rubicola， Plectosphaerella cucumerina， Dactylonectria alcacerensis and Fusarium solani showed higher abundance. The functional analysis indicated the above-mentioned bacterial genera may realize rapid proliferation by utilizing， biodegrading and transforming phenylethanoid glycosides， and some potential fungal pathogens were colonized. ConclusionThe R. glutinsoa-soil feedbacks were likely generated by the phenylethanoid glycosides in the root exudates together with the specific rhizomicrobes. The investigations of R. glutinsoa-soil feedbacks under continuous cropping system are critical to the further understanding of the underlying mechanisms related to its continuous cropping obstacles.

Objective To explore the causal relationship between family resilience and mental health in military personnel population.Methods A total of 204 military personnel were recruited from an army unit stationed in Western China with cluster convenience sampling.Family Resilience Scale(FRS)and Symptom Checklist 90(SCL-90)were used to survey them twice,in an interval of 4 months.Amos 26.0 was applied to construct a cross-lag model and analyze the data.Results After controlling mental symptoms from the first survey,family resilience in the first measure significantly predicted mental symptoms in the second measure(β=-0.14,P＜0.05).After controlling for family resilience from the first survey,mental symptoms in the first measure significantly predicted family resilience in the second measure(β=-0.13,P＜0.05).Conclusion The relationship between family resilience and mental health is mutually causal in military personnel,and one predicts the other one.Our findings highlight the key dimensions of the relationship between the two.

ObjectiveTo study the composition of volatile oil in Opisthopappus taihangensis （Taihangju）， and provide a reference for comprehensive development of this medicine. MethodTaking Chrysanthemum morifolium （Xiaobaiju）， C. morifolium （Xiaohuangju） and C. indicum （Yejuhua） as control， the qualitative and quantitative analysis of volatile oil in Taihangju and three control varieties were completed by gas chromatography-mass spectrometry （GC-MS） combined with retention index method. The GC conditions were as following：programmed temperature （initial temperature at 60 ℃， kept for 2 min； up to 120 ℃ with the heating rate of 5 ℃·min^-1， still kept for 2 min； up to 180 ℃ with the heating rate of 2 ℃·min^-1， kept for 3 min； and then up to 240 ℃ with the heating rate of 8 ℃·min^-1， kept for 5 min； finally up to 280 ℃ with the heating rate of 10 ℃·min^-1， kept it for 5 min and finished）， high-purity helium as the carrier gas， the split ratio of 50∶1. MS conditions were as follows：electron impact ion source （EI）， ion source temperature of 230 ℃， electron collision energy of 70 eV and scanning range of m/z 30-445. Principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were used to obtain the characteristic components between Taihangju and the three control varieties. ResultA total of 86， 96， 112 and 109 compounds including 73 common components were identified in Taihangju， Xiaobaiju， Xiaohuangju and Yejuhua， respectively. The contents of volatile components in Taihangju were significantly different from that of the control varieties. In which， the relative contents of α-thujone， eucalyptol and terpinen-4-ol were high in Taihangju， and eucalyptol， camphor and α-terpinyl acetate were the main compositions in the control varieties. In addition， 11 compounds were screened as characteristic components to distinguish Taihangju and the three control varieties by PCA and OPLS-DA， including main differential components of chamazulene and δ-cadinene. ConclusionThe main components of volatile oil in Taihangju includes alcohols， terpenes， ketones and esters with high medicinal value. The accuracy of qualitative analysis of volatile oil is improved by GC-MS combined with retention index method， which provides scientific reference for development and utilization of Taihangju.

This paper was aimed to explore the relationship between different commercial specification and chemical constituents on Radix Rehmanni,which provided experimental evidences for drawing up the commodity commercial specification and grading criteria of Radix Rehmanni.Several classified indexes,including grade,surface color,section color,section texture were carried to explore the change rules of chemical constituents by setting the contents of iridoid glycoside,sugars,water content,total ashes,acid-insoluble ashes and water extract as the markers.The results showed that the grade,surface color and section texture had less impact on chemical constituents.However,the section color had more influence.Along with the section color gradually being deepened,the contents of iridoid glycoside and oligosaccharides of Radix Rehmanni were lower,and the contents of monosaccharide and polysaccharide were higher.It was concluded that there was correlation between commercial specification and chemical constituents of Radix Rehmanni.

Objective To discuss the effect of measuring central venous with peripherally inserted central catheter (PICC) in low birth weight infants (LBWIs).Methods Totally 100 LBWIs admitted in the intensive care unit ( ICU) of a tertiary hospital from January to Octomber 2015 were selected and were randomly divided into the PICC group and the central venous catheterization ( CVC) group, with 50 cases in each group. Patients in the PICC group received the main intravenous catheter, while patients in the CVC group received subclavian intravenous catheter. Data were collected for statistical analysis. The difference of central venous pressure and the incidence rate of complications in patients with different measure methods were compared between two groups.Results The central venous pressure of LBWIs in the PICC group was (8.26±2.18) cmH2O, and in the CVC group was (7.85±2.36) cmH2O (t=0.421,P>0.05); the incidence rates of catheter obstruction, phlebitis and puncture point bleeding of the PICC group were lower than that of the CVC group (χ2=5.01,6.93, 4.00;P<0.05).Conclusions PICC catheter measurement won′t affect the measure results of central venous pressure in LBWIs,and can reduce the incidence of complications related with central venous catheter in very low LBWIs, so it can be popularized for use.

Objective To explore the risk factors of cognitive impairment of elderly patients with cerebral infarction in order to provide the theoretical basis for the clinical intervention. Methods A total of 237 cases with senile cerebral infarction were selected as ours subjects who were hospitalized from Mar. 2010 to Jun. 2013 in Kailuan General Hospital Affiliated to Hebei United University. The general condition and medical history were recorded. The auxiliary examination was performed. Cerebral infarction was diagnosed based on the onset to diagnosis standard and MoCA scores of within 2 weeks. The patients with less than 26 MoCA score were diagnosed as cognitive dysfunction and otherwise were thought as normal. Single factor analysis methods and non conditional Logistic regression were applied to analyze the analysis. Results There was no significant difference in terms of incidence between patients with different gender. Patients with age more than 75 years old and lower education levels had the high incidence rate than those with younger age and high education levels( χ2=16. 661,5. 453;P﹤0. 05). The cognitive dysfunction incidence of patients with white collar was lower than those with blue collar(χ2 = 5. 458,P ﹤ 0. 05 ). And the cognitive dysfunction incidence of patients with hypertention,diabetes,heart disease and leukoaraiosis were higher than those without the above diseases(χ2 =28. 423,5. 621,7. 768,6. 070;P﹤0. 05). The incidence of patients smoking more was significantly higher than that of smoking less or no(χ2 =5. 045,P ﹤0. 05 ). Multiple factors and non conditional Logistic regression analysis showed that,67 Senile cerebral infarction patients occurred cognitive impairment within 2 weeks. The independent risk factors for its occurrence included age greater than 75 years( P=0. 000 ),diabetes mellitus( P=0. 043),hypertension(P=0. 000)and leukoaraiosis(P=0. 041). Conclusion There are many risk factors related to cognitive impairment after cerebral infarction occurred in the elderly. The intervention should take in many aspects and the risk factors should early found.

To analyze the molecular mechanisms of cross-host transmission of the Aleutian mink disease vi rus (ADV), the hypervariable region fragment of the VP2 gene of the ADV in Jilin Province (China) was amplified. Sequencing analyses showed diversity at residue 174 by comparison with other VP2 genes in GenBank. The phylogenetic tree indicated that the ADV-JL strain had a close relationship with the highly pathogenic strain from Denmark: ADV-K. Results implied that residue 174 may be associated with ADV infectivity.
Aleutian Mink Disease ; virology ; Aleutian Mink Disease Virus ; chemistry ; classification ; genetics ; isolation & purification ; Amino Acid Sequence ; Animals ; Capsid Proteins ; chemistry ; genetics ; China ; Mink ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Analysis

Objective To investigate the application effect of centralized health education among low-income parents in neonatal intensive care unit ( NICU) . Methods A total of 200 cases, who had low income parents and admitted to NICU from December 2012 to June 2013, were randomly divided into observation group (100 cases) and control group (100 cases). The neonatus in the control group underwent conventional health education mode, while neonatus in the observation group received conventional health education model based on a new type of health education on adoption. The awareness of health education, medical compliance and satisfaction of nursing assessed by self-designed health education effect scale, self-realization medical compliance and the satisfaction questionnaire in our hospital for quality of care. Results The satisfaction for quality of care in the experimental group was 97. 0% higher than 84. 0% in the control group (χ2 =9. 83,P<0. 01). The knowledge control of parents after health education in the experimental group was better than that in the control group (P<0. 01). Similarly, the medical compliance of neonatal parents in the experimental group was better than that of control group including treatment (92. 0%), treatment cooperation (95. 0%), payment on time (100. 0%) and visit as required (90.%) (χ2 =20. 37,20. 41,18. 58,14. 59, respectively;P <0. 01). Conclusions The centralized health education can facilitate the knowledge control of low-income parents in NICU, satisfaction of health education and medical compliance, and is worth of clinical promotion.

Objective To explore the application of PDCA cycle on reducing unplanned extubation ( UEX) incidence of invasive arterial blood pressure monitoring in child patients. Methods The PDCA cycle had been used to documented the UEX status of invasive arterial blood pressure monitoring in 2013, analyses the reason of UEX, set improvement goals, formulate measures, organize and implement the strategy, and compared and analyses the improved effect. Results After the introduction of PDCA cycle management approach, the experimental group of arterial indwelling catheter UEX rate (4. 5%) was lower than that of the observation group (15. 45%) (χ2 =6. 62,P <0. 05); for children of experimental group, the length of hospitalization was (22. 98 ± 2. 53) d, which was lower than that of the control group (22. 98 ± 2. 53) d (t=12. 846,P<0. 05), the expenditure of hospitalization of the control group (6 991. 1 ± 237. 1) yuan was higher than that observation group(5849.71±252.80) yuan(t=34.631,P<0.05).Conclusions ThePDCAnursingcanpreventand control the UEX rate of invasive arterial blood pressure monitoring standardize nursing operation, and improve nursing management level.

Objective To explore the application effect of traditional and gravity nasogastric feeding of preterm infants in neonatal intensive care unit ( NICU) .Methods Eighty preterm infants whose gestational age was less than 34 weeks and birth weight was lower than 2 000 g from August 2012 to June 2013 were chosen through the prospective randomized controlled study method and were randomly divided into the control group and the observation group according to the hospitalization number , each with 40 cases.The control group received the traditional nasogastric feeding , and the observation group received the gravity nasogastric feeding . The time of achieving the total parenteral nutrition and hospitalization , the tolerance for feeding and the related complication were compared between two groups .Results The time of achieving the total parenteral nutrition and hospitalization in the observation group were significantly better than those of control group , and the differences were statistically significant (t=4.780, 8.846, respectively;P<0.05).The tolerance for feeding in the observation was higher than that of the control group , and the cases of apnea in the observation group were less than those of the control group , and the differences were statistically significant (χ2 =3.19, 9.69, respectively;P <0.05).No differences were found in the abdominal distension, diarrhea, necrotizing enterocolitis between two groups (χ2 =0.11, 0.21, respectively;P>0.05).Conclusions Gravity nasogastric feeding can increase the tolerance for feeding in the preterm infants , and shorten the time of achieving the total parenteral nutrition and hospitalization in order to lay a good foundation for growth and development of preterm infants.