Long non-coding RNA myocardial infarction related transcript(MIAT)is a widely studied lncRNAs,which is closely related to the occurrence and development of many diseases.MIAT is associated with inflammatory responses in a variety of dis-eases through further exploring,including diabetes and its complications,cardiovascular and osteoarticular diseases.However,sys-tematic knowledge of the relationship between MIAT molecules and inflammation in diseases remains unclear.This article will focus on the relationship between MIAT and inflammation in various systemic diseases,especially its role,mechanism and potential clinical ap-plication as a biomarker and therapeutic target of disease inflammation,in order to provide molecular strategies for diagnosis and treat-ment of the inflammatory diseases.

Long non-coding RNA myocardial infarction related transcript(MIAT)is a widely studied lncRNAs,which is closely related to the occurrence and development of many diseases.MIAT is associated with inflammatory responses in a variety of dis-eases through further exploring,including diabetes and its complications,cardiovascular and osteoarticular diseases.However,sys-tematic knowledge of the relationship between MIAT molecules and inflammation in diseases remains unclear.This article will focus on the relationship between MIAT and inflammation in various systemic diseases,especially its role,mechanism and potential clinical ap-plication as a biomarker and therapeutic target of disease inflammation,in order to provide molecular strategies for diagnosis and treat-ment of the inflammatory diseases.

Background:There is no gold standard for the diagnosis of ulcerative colitis(UC).Given that microRNAs(miRNAs)have been shown to play an important role in inflammation,cell death,and cancer progression,detection of relevant serum markers may be valuable in the diagnosis of UC.Aims:To analyze the expression and clinical significance of miR-182-5p and miR-142-5p in the serum of patients with UC.Methods:From May 2021 to May 2023,86 UC patients admitted to our hospital were collected.According to the clinical severity,UC patients were grouped into mild group,moderate group,and severe group.86 healthy volunteers(control group)who underwent physical examinations in our hospital were collected.qRT-PCR was applied to detect serum levels of miR-182-5p and miR-142-5p.Spearman method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in the serum of UC patients and the Mayo score;;Pearson method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in serum of UC patients and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6.ROC curve was applied to analyze the diagnostic value of serum miR-182-5p and miR-142-5p for UC.Decision curve analysis(DCA)was used to determine the clinical practicability of serum miR-182-5p and miR-142-5p and combined detection in the diagnosis of UC.Results:Compared with the control group,the expression levels of serum miR-182-5p,TNF-α,IL-1β,and IL-6 in the UC group were greatly increased,while the expression level of miR-142-5p was greatly reduced(P＜0.05),there was no great difference in IL-10 level between the two groups(P＞0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with TNF-α,IL-1β,and IL-6 levels,while the expression level of serum miR-142-5p was negatively correlated with TNF-α,IL-1β,and IL-6 levels(P＜0.05).Compared with the mild group,the expression level of miR-182-5p and Mayo score in the serum of UC patients in the moderate and severe groups increased greatly,while the expression level of miR-142-5p decreased greatly(P＜0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with the Mayo score(rs=0.774,P＜0.001);the expression level of serum miR-142-5p was negatively correlated with Mayo score(rs=-0.523,P＜0.001).The AUC value of miR-182-5p combined miR-142-5p in diagnosing UC was higher than that of miR-182-5p and miR-142-5p alone(P＜0.05).The results of DCA curve showed that when the high risk threshold was 0.08-0.95,the net benefit rate of the combined detection of serum miR-182-5p and miR-142-5p in the diagnosis of UC was better than that of miR-182-5p and miR-142-5p alone.Conclusions:The expression level of miR-182-5p in serum of UC patients was significantly increased,and the expression level of miR-142-5p was significantly decreased,both of which could be used as indicators for observing the disease activity of UC.

Background:There is no gold standard for the diagnosis of ulcerative colitis(UC).Given that microRNAs(miRNAs)have been shown to play an important role in inflammation,cell death,and cancer progression,detection of relevant serum markers may be valuable in the diagnosis of UC.Aims:To analyze the expression and clinical significance of miR-182-5p and miR-142-5p in the serum of patients with UC.Methods:From May 2021 to May 2023,86 UC patients admitted to our hospital were collected.According to the clinical severity,UC patients were grouped into mild group,moderate group,and severe group.86 healthy volunteers(control group)who underwent physical examinations in our hospital were collected.qRT-PCR was applied to detect serum levels of miR-182-5p and miR-142-5p.Spearman method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in the serum of UC patients and the Mayo score;;Pearson method was applied to analyze the relationship between the expression levels of miR-182-5p and miR-142-5p in serum of UC patients and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6.ROC curve was applied to analyze the diagnostic value of serum miR-182-5p and miR-142-5p for UC.Decision curve analysis(DCA)was used to determine the clinical practicability of serum miR-182-5p and miR-142-5p and combined detection in the diagnosis of UC.Results:Compared with the control group,the expression levels of serum miR-182-5p,TNF-α,IL-1β,and IL-6 in the UC group were greatly increased,while the expression level of miR-142-5p was greatly reduced(P＜0.05),there was no great difference in IL-10 level between the two groups(P＞0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with TNF-α,IL-1β,and IL-6 levels,while the expression level of serum miR-142-5p was negatively correlated with TNF-α,IL-1β,and IL-6 levels(P＜0.05).Compared with the mild group,the expression level of miR-182-5p and Mayo score in the serum of UC patients in the moderate and severe groups increased greatly,while the expression level of miR-142-5p decreased greatly(P＜0.05).Correlation analysis showed that the expression level of serum miR-182-5p was positively correlated with the Mayo score(rs=0.774,P＜0.001);the expression level of serum miR-142-5p was negatively correlated with Mayo score(rs=-0.523,P＜0.001).The AUC value of miR-182-5p combined miR-142-5p in diagnosing UC was higher than that of miR-182-5p and miR-142-5p alone(P＜0.05).The results of DCA curve showed that when the high risk threshold was 0.08-0.95,the net benefit rate of the combined detection of serum miR-182-5p and miR-142-5p in the diagnosis of UC was better than that of miR-182-5p and miR-142-5p alone.Conclusions:The expression level of miR-182-5p in serum of UC patients was significantly increased,and the expression level of miR-142-5p was significantly decreased,both of which could be used as indicators for observing the disease activity of UC.

Objective:To investigate the clinical effect and action mechanism of Smecta and Kangfuxin mixture retention enema combined with hyperbaric oxygen (HBO) in the prevention of radiation proctitis (RP) after radiotherapy for cervical cancer.Methods:A total of 92 patients with RP after radiotherapy for cervical cancer admitted to Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2016 to December 2019 were selected and divided into observation group and control group according to their treatments, with 46 cases each. The control group was treated with Smecta and Kangfuxin mixture retention enema, and the observation group was treated with HBO on the basis of the treatment of the control group. The incidence, time of occurrence, and classification of acute and chronic RP of both groups were recorded. The results of colonoscopy were observed and evaluated by the Viennese colonoscopy score. The levels of immunoglobulin IgG, IgM, and IgA in the two groups before and after treatment were detected by immunoturbidimetric method. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of C-reactive protein (CRP) was detected by fluorescence method.Results:The incidence of acute RP after radiotherapy in the observation group was significantly lower than that in the control group, and the average occurrence time of RP in the observation group was significantly delayed than that in the control group ( P<0.05). The incidences of grade 1-2, grade 3-4 RP, and the total incidence of acute and chronic RP in the observation group were significantly lower than those in the control group ( P<0.05). The patients with 0-2 points of Viennese colonoscopy scores in the observation group were significantly more than those in the control group, and the patients with 3-5 points were less than those in the control group, the differences were statistically significant ( P<0.05). After treatment, the levels of IgG, IgM, and IgA in the observation group were significantly higher than those in the control group, while the levels of TNF-α, IL-6, and CRP in the observation group were significantly lower than those in the control group ( P<0.05). Conclusion:Smecta and Kangfuxin mixture retention enema combined with Hyperbaric oxygen (HBO) can delay and reduce the occurrence of radiation proctitis and relieve the clinical symptoms of cervical cancer patients after radiotherapy. The mechanism may be achieved by improving the immune function and reducing the level of inflammatory factors.

Objective:To investigate the clinical effect and action mechanism of Smecta and Kangfuxin mixture retention enema combined with hyperbaric oxygen (HBO) in the prevention of radiation proctitis (RP) after radiotherapy for cervical cancer.Methods:A total of 92 patients with RP after radiotherapy for cervical cancer admitted to Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2016 to December 2019 were selected and divided into observation group and control group according to their treatments, with 46 cases each. The control group was treated with Smecta and Kangfuxin mixture retention enema, and the observation group was treated with HBO on the basis of the treatment of the control group. The incidence, time of occurrence, and classification of acute and chronic RP of both groups were recorded. The results of colonoscopy were observed and evaluated by the Viennese colonoscopy score. The levels of immunoglobulin IgG, IgM, and IgA in the two groups before and after treatment were detected by immunoturbidimetric method. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of C-reactive protein (CRP) was detected by fluorescence method.Results:The incidence of acute RP after radiotherapy in the observation group was significantly lower than that in the control group, and the average occurrence time of RP in the observation group was significantly delayed than that in the control group ( P<0.05). The incidences of grade 1-2, grade 3-4 RP, and the total incidence of acute and chronic RP in the observation group were significantly lower than those in the control group ( P<0.05). The patients with 0-2 points of Viennese colonoscopy scores in the observation group were significantly more than those in the control group, and the patients with 3-5 points were less than those in the control group, the differences were statistically significant ( P<0.05). After treatment, the levels of IgG, IgM, and IgA in the observation group were significantly higher than those in the control group, while the levels of TNF-α, IL-6, and CRP in the observation group were significantly lower than those in the control group ( P<0.05). Conclusion:Smecta and Kangfuxin mixture retention enema combined with Hyperbaric oxygen (HBO) can delay and reduce the occurrence of radiation proctitis and relieve the clinical symptoms of cervical cancer patients after radiotherapy. The mechanism may be achieved by improving the immune function and reducing the level of inflammatory factors.

Post-traumatic stress disorder ( PTSD) is a serious psychiatric disorder when someone suffered from a major trauma, next followed by sleep disorder, emotional and cognitive disorder and other symptoms. Over the past few decades, many stress rodents models have been developed for searching the potential pathophysiological pathways of PTSD. All models showed PTSD-like symptoms, but none of them could manifestate all the symptoms and biological changes of PTSD completely. Thus, this article makes a brief summary about the PTSD models commonly used in recent years.

Antigens, Neoplasm ; metabolism ; Antigens, Surface ; metabolism ; Colorectal Neoplasms ; immunology ; pathology ; Humans ; Prognosis ; Trophoblasts ; immunology

Objective To observe the effects of electroacupuncture on the expressions of autophagy related protein Beclin-1 and apoptosis related protein p53 of hippocampus in rats;To explore the mechanism of electroacupuncture on Alzheimer's disease (AD).Methods The rats were randomly divided into the normal group, the sham-operation group, the model group, and the electroacupuncture treatment group. “Baihui” and “Yongquan” points were taken for electroacupuncture treatment and the treatment course was 7 days. The rats were treated once a day for 4 courses. Changes in morphology and number of Nissl positive cells were examined by Nissl staining in hippocampal CA1 regions. Expressions of Beclin-1 and p53 protein were determined by Western blot analysis.Results Number of Nissl positive cells in CA1 region of the model group was significantly less than that of normal group (P<0.01). After electroacupuncture treatment, number of pyramidal cells and expression of Nissl body significantly increased (P<0.05). Expression of Beclin-1 decreased, while expression of p53 increased in the hippocampus of the model group, compared with that in the normal group (P<0.05). However, electroacupuncture treatment could significantly upregulate the expression of Beclin-1 protein (P<0.01), but downregulate the level of p53 (P<0.05).Conclusion Electro-acupuncture treatment could fight against Aβ-induced neuronal apoptosis, and improve the morphological changes of AD’s hippocampus.

Objective To explore the protective effects of electro-acupuncture (EA) serum onβ-amyloid protein (Aβ) induced primary rat hippcampal neurons. Methods The rat models of Alzheimer's disease were established by intracerebral injection of Aβ1-40. After treated them with EA, the serum was harvested. Primary cultured hippocampal neurons were treated with Aβ25-35 to establish neuronal damage model in vitro, and divided into normal group, model group and EA serum group. The proliferation of neurons was detected by MTT test. Neuronal apoptosis was examined by TUNEL staining, and expression of cysteine aspartic acid proteases-3 (Caspase-3) was detected by immunocytochemical staining. Results MTT test showed that the cell viability was significantly decreased after treated with Aβ. While compared with the model group, cell proliferation of EA serum group was significantly enhanced (P<0.01). TUNEL staining showed that the number of apoptotic cells in EA serum group decreased significantly compared with the model group (P<0.01). After 48 h of Aβ treatment, Caspase-3 expression levels were significantly elevated. However, compared with the model group, the number of Caspase-3 positive cells in EA serum group was significantly reduced (P<0.01). Conclusion The EA serum could promote the proliferation of hippocampal neurons, reduce the expression of Caspase-3, counteract the neurotoxicity of β-amyloid protein, and reduce neuronal apoptosis.