Objective:To determine the relative correction factor of pre-vitamin D and simplify the calculation method of vitamin D assay.Methods:By studying the calculation method of vitamin D content in drug standards of various countries,HPLC was used to determine the relative correction factor of pre-vitamin D,and the influencing factors of determination were investigated.Results:The relative correction factors of pre-vitamin D at 254 nm and 265nm wavelength were determined by statistical analysis of 7 laboratories in China.Conclusion:Using the pre-vi-tamin D relative correction factor method to calculate the total amount of vitamin D simplified the experimental steps can be simplified by the pre-vitamin D relative correction factor method to calculate the total amount of vitamin D and the random operating errors can be avoided.The method is rapid and accurate,and lay a solid foundation for further improving the standard of vitamin D preparations.

It was previously believed that hepatic portal venous gas (HPVG) was an "ominous sign" or "death sign", and once it appeared, the disease progressed rapidly with a high mortality rate which required immediate surgical treatment. However, with the continuous progress of medical technology, researchers have gained a deeper understanding that various causes can lead to HPVG, not all of which required surgical treatment, and the prognosis was not poor. Unfortunately, there is no guideline or consensus on the diagnosis and treatment of HPVG to guide clinical management and standardize diagnostic and therapeutic behaviors. Therefore, the authors review previous literatures with combined clinical diagnosis and treatment experience to conduct profound discussion on the epidemiological features, etiology, pathogenesis, imaging features, treatment strategies, and progno-sis of HPVG, and develop corresponding diagnosis and treatment procedures with the aims to help clinicians to improve diagnostic and therapeutic outcomes and prognosis of patients.

It was previously believed that hepatic portal venous gas (HPVG) was an "ominous sign" or "death sign", and once it appeared, the disease progressed rapidly with a high mortality rate which required immediate surgical treatment. However, with the continuous progress of medical technology, researchers have gained a deeper understanding that various causes can lead to HPVG, not all of which required surgical treatment, and the prognosis was not poor. Unfortunately, there is no guideline or consensus on the diagnosis and treatment of HPVG to guide clinical management and standardize diagnostic and therapeutic behaviors. Therefore, the authors review previous literatures with combined clinical diagnosis and treatment experience to conduct profound discussion on the epidemiological features, etiology, pathogenesis, imaging features, treatment strategies, and progno-sis of HPVG, and develop corresponding diagnosis and treatment procedures with the aims to help clinicians to improve diagnostic and therapeutic outcomes and prognosis of patients.

Objective To analyze the epidemiological features of acute carbon monoxide poisoning (ACOP) in children induced by coal burning for home-heating, so as to provide evidence for ACOP prevention and control.Methods A retrospective analysis was made on the medical data of gender , age, dwelling place, poisoning time, intoxication of family members , implementation time of hyperbaric oxygen ( HBO) therapy in child patients with ACOP admitted to the Department of HBO of Tianjin Children's Hospital from June 1, 2012 to May 31, 2017 due to coal burning for home-heating in Tianjin area from 2012 to 2017.Results In the past 5 years, a total of 381 patients with ACOP were admitted for treatment , including 322 patients intoxicated (84.51％)owing to burning coal for home-heating.Of the 322 patients, 181 were boys (56.21％) and 141 were girls (43.79％).Age ranged from 22 days to 15 years old, with an average age of 4.2 ±3.3 years.And of the 322 patients, 73 were younger than 1 year old (22.67％), 85 were in the age of 1-3 years (26.40％), 91 were in the age of 3-6 years (28.26％), and 73 were over 6 years old (22.67％).As to the area of distribution of ACOP, 37 cases were in 6 main municipal districts of Tianjin , accounting for (11.49％), 166 cases were in the suburban , accounting for 51.55％and 119 cases were in exurban of the city , accounting for 36.96％, with an annual average incidence of 64.4 cases.Most cases ( 281, 87.27％) occurred in the centralized heating period, i.e.from Nov 15 to Mar 15 nest year, and relatively few cases (41, 12.73％) occurred beyond the heating period .Beyond the heating period , the percentage of ACOP patients younger than 1 year was 29.27％(12/41), and statistical significance could be noted when comparisons were made (P=0.004).There were 280 ACOP patients (86.96％) who had ACOP at night (86.96％), and there were 272 patients (84.47％) whose family members also had ACOP , with a total 489 family members having ACOP . One hundred and forty-one patients (43.79％) received HBO therapy , 6 hours after the onset of symptoms of carbon monoxide poisoning , and on the average , they had 6.1 sessions of HBO therapy .Conclusion In the highly prevalent city areas , during the winter season and among special population of the city , propaganda and early warning concerning knowledge of carbon monoxide poisoning should be made , so as reduce the rate of ACOP.

Objective To analyze the epidemiological features of acute carbon monoxide poisoning (ACOP) in children induced by coal burning for home-heating, so as to provide evidence for ACOP prevention and control.Methods A retrospective analysis was made on the medical data of gender , age, dwelling place, poisoning time, intoxication of family members , implementation time of hyperbaric oxygen ( HBO) therapy in child patients with ACOP admitted to the Department of HBO of Tianjin Children's Hospital from June 1, 2012 to May 31, 2017 due to coal burning for home-heating in Tianjin area from 2012 to 2017.Results In the past 5 years, a total of 381 patients with ACOP were admitted for treatment , including 322 patients intoxicated (84.51％)owing to burning coal for home-heating.Of the 322 patients, 181 were boys (56.21％) and 141 were girls (43.79％).Age ranged from 22 days to 15 years old, with an average age of 4.2 ±3.3 years.And of the 322 patients, 73 were younger than 1 year old (22.67％), 85 were in the age of 1-3 years (26.40％), 91 were in the age of 3-6 years (28.26％), and 73 were over 6 years old (22.67％).As to the area of distribution of ACOP, 37 cases were in 6 main municipal districts of Tianjin , accounting for (11.49％), 166 cases were in the suburban , accounting for 51.55％and 119 cases were in exurban of the city , accounting for 36.96％, with an annual average incidence of 64.4 cases.Most cases ( 281, 87.27％) occurred in the centralized heating period, i.e.from Nov 15 to Mar 15 nest year, and relatively few cases (41, 12.73％) occurred beyond the heating period .Beyond the heating period , the percentage of ACOP patients younger than 1 year was 29.27％(12/41), and statistical significance could be noted when comparisons were made (P=0.004).There were 280 ACOP patients (86.96％) who had ACOP at night (86.96％), and there were 272 patients (84.47％) whose family members also had ACOP , with a total 489 family members having ACOP . One hundred and forty-one patients (43.79％) received HBO therapy , 6 hours after the onset of symptoms of carbon monoxide poisoning , and on the average , they had 6.1 sessions of HBO therapy .Conclusion In the highly prevalent city areas , during the winter season and among special population of the city , propaganda and early warning concerning knowledge of carbon monoxide poisoning should be made , so as reduce the rate of ACOP.

Objective To investigate the related factors of clinical stage and prognosis in the patients with endometrial carcinoma and their relation with proto-oncogene Wip1 expression level.Methods The paraffin samples of resected endometrial carcinoma in 120 cases of endometrial carcinoma in our hospital from January 2002 to January 2012 were collected as the experimental group,the samples were verified by pathology.Contemporaneous 120 samples of biopsy normal endometrial tissue served as the control group.The expression leve of Wipl were detected in turo groups.Results (1) In the Wip1 immunohistochemical staining results:Wip1 immunohistochemical staining was negative or weak in normal endometrial tissue cells,while showed pale yellow to yellowish-brown in endometrial cancer tissue.The positive expression rate of Wip1 protein in endometrial carcinoma tissue was 77.5%(93/120),which was higher than 22.5%(27/120) in normal endometrial tissue,the difference was statistically significant (P<0.05).(2)In the Western blot results of Wip1 protein in endometrial cancer tissue and normal endometrial tissue:the relative amount of Wip1 protein in endometrial carcinoma tissue was 0.635±0.023,which was significantly higher than 0.325±0.018 in normal endometrial tissue,the difference between the two groups was statistically significant (P<0.05).(3)In the real time quantitative qRT-PCR results of various samples:Wip1 mRNA expression level was higher than that in normal endometrial tissue,which were 0.628±0.053 and 0.191±0.009 respectively,the difference between the two groups was statistically significant(P<0.05).(4) The expression level of Wip1 had no correlation with age,estrogen and progesterone status,HER2,lymph node status and TNM stage,but had correlation with P53 expression level.Conclusion (1) The Wip1 expression amount is high in endometrial carcinoma and low in normal endometrial tissue.(2)The Wip1 expression level has no relation with age,estrogen and progesterone status,HER2,lymph node status and TNM stage,while has association with P53 expression level.

Objective To observe the outcomes of the modified multiple nerve transfer s combined with the late hand function reconstruction to restore the active pick-up function of the paralyzed upper extremity in patients with total brachial plexus avulsion injuries (TBPAI).Methods 33 patients suffered with TBPAI firstly underwent multiple nerve transfers,which including accessory nerve transfers to neurotize the suprascapular nerve to recover the shoulder abduction,contralateral C7 (CC7) nerve transfers via the modified pre-spinal route with direct coaptation to restore lower trunk function and the musculocutaneous nerve was also neurotized by the transferred CC7 nerve via a cutaneous nerve graft to restore the function of elbow flexion,as well as the phrenic nerve transfers to neurotize the posterior division of lower trunk to restore the function of elbow and finger extension.The patients with muscle recovery were selected to perform the hand function reconstruction at the second stage for restoring the active pick-up function.The patients were chosen as followcriterias:the degree of shoulder abduction attained 30°or more,the motor power of elbow,wrist,and finger flexion attained grade M4 or more,elbow and finger extension attained M3 or more.The methods of hand function reconstruction included wrist fusion and flexor carpal ulnaris opponensplasty,in addition to palmar capsulodesis of the metacarpophalangeal joint.Results The mean follow up was 41±7.7 (range,36-73 months) after the first procedure of multiple nerve transfers,the muscle strength of elbow and finger and wrist flexion attained M 4 as well as the elbow and finger extension achieved M3 or more in 10 patients,all of 10 patients achieved 40°-80°shoulder abduction.8 out of 10 patients had performed the second surgical procedure for hand functional reconstruction.6 of them had successfully recovered the active pick-up function.Conclusions The newly designed procedure of multiple nerve transfers could effectively restore the function of shoulder abduction,elbow,wrist,and finger flexion,as well as elbows and finger extension in patients with TBPAI,combined with the hand functional reconstruction,active pick-up function could be successfully reconstructed.

ObjectiveTo classify the type of lumbosacral plexus nerve root injury.MethodsFrom November 2004 to August 2011,36 patients suffered with lumbarsacral plexus nerve root injury underwent surgical exploration in our department.There were 24 males and 12 females,aged from 7 to 49 years(average,29.5 years).By inductively analyzing the location and amount of nerve root injury,preoperative clinical manifestations and results of physical examination,the clinical typing of lumbarsacral plexus nerve root injury was made.ResultsLumbosacral plexus nerve root injury was classified into 6 types:total lumbosacral plexus nerve root injury (4 cases),lumbar plexus and upper sacral plexus nerve root injury (6 cases),sacral plexus nerve root injury (9 cases),upper sacral plexus nerve root injury (11 cases),lower sacral plexus nerve root injury(4 cases) and lumbar plexus injury(2 cases).There were 19 patients with total lumbosacral plexus nerve root injury,lumbar plexus and upper sacral plexus nerve root injury or sacral plexus nerve root injury,among which 73.7％(14/19) nerve root injury located in the spinal canal and all of them were nerve root avulsion or rupture.There were 17 patients with upper sacral plexus nerve root injury,lower sacral plexus nerve root injury or lumbar plexus nerve root injury,among which 64.7％ (11/17) nerve root injury located in intro-pelvic or pelvic sacral foramina,and all of them were distraction injury.ConclusionThis clinical typing is useful for the accurate diagnosis of lumbosacral plexus nerve root injury.In addition,it is also beneficial for judging the location and characteristics of nerve root injury.

Objective To develop a scale of social adaptation degrees of special service personnel (referred to as scale) applying Traditional Chinese Medicine and psychological theory and to assess its reliability and validity. Methods By literature review, job analysis, inventory survey, topics interview, this scale was determined in dimensions, items and items pool based on theoretical model. Results After specialist important analysis and combining the different results of statistical analysis, including Cronbach's α coefficient, resolution factor, correlation analysis and confirmatory factor analysis, 6 dimensions, 14 factors and 40 items were finally kept in the scale. The dimensions were psychological characteristics, physical condition, intelligence level, interpersonal relationships, environmental factors and moral character. The factors were self-control, self-esteem character bias, self-confidence, physiological function, learning intelligence, creating intelligence, leadership intelligence, gregariousness, cooperative, confidence feeling, natural environment, social environment and moral character. Conclusion This scale was made on the basis of general international principle, it had good reliability and validity meeting measurement standard of traditional Chinese medicine and psychological theory, and it is an effective and practical evaluation tool of social adaptation degrees of special service personnel.

Objective To explore a safe and effective hyperthermia interventional therapy temperature for hepatic carcinoma. Methods Eight swines were divided into four groups according to trans-arterial hyperthermic perfusion temperature, 45 ?C , 50 ?C ,55 ?C , and 60 ?C (catheter flowing temperature) groups. The hepatic and renal functions and blood coagulation function were examined before and after the procedur and then all swines were sacrificed and the livers were pathologically analysed,simultoneously with the evaluation of the safe hyperthermic temperature 35 hepatic carcinomas were carried out under this interventional hyperthermochemotherapy via the arterial catheter (80 cm, 5F) placed into the tumoral artery with the perfusion agents warmed to 60～65 ?C (catheter flowing temperature was 47.55?0.44 ?C ). Results The hyperthermic coagulation necrosis, hepatic dysfunction level and fever were found after 55 ?C and 60 ?C hyperthermic perfusion while those of 45 ?C and 50 ?C groups basically remained normal. The tumor growth rate and total efficacy rate were -(0.35?0.32) and 79.2%, respectively, and 0.5, 1, 1.5 year survival rates were 100%, 80%, 60% respectively after follow-up. The adverse effects of the interventional hyperthermochemotherapy was similar to the routine TACE. Conclusions 45 ?C and 50 ?C (catheter flowing temperature) hyperthermochemotherapy for hepatic carcinoma is safe and effective.