Objective:To explore the value of transvaginal ultrasound (TVS) combined with neutrophil to lymphocyte ratio (NLR) in postmenopausal women with endometrial cancer(EC).Methods:A retrospective analysis was conducted on 348 postmenopausal patients who underwent hysteroscopy or surgery in the Obstetrics and Gynecology Hospital of Fudan University from January 2019 to December 2021 and had pathological results of EC, and they were included in the malignant group. One hundred and six benign patients with pathological results in the same period were randomly selected for inclusion in the benign group. The differences in age, menopausal time, gravidity, body mass index (BMI), post-menopausal bleeding, TVS and NLR were compared between the two groups. Binary Logistic regression analysis was performed to determine the independent predictive factors for endometrial cancer in postmenopausal women. ROC curves were plotted to evaluate the diagnostic efficacy of predicting EC in postmenopausal women.Results:Parity (≥2 times), post-menopausal bleeding, intrauterine fluid, the diameter of the mass, blood flow and NLR showed statistically significant differences between the malignant group and the benign group (all P<0.05). Logistic regression analysis showed that post-menopausal bleeding, intrauterine fluid and long diameter of the mass were independent predictors for EC in postmenopausal women( OR=0.433, P=0.006; OR=14.933, P<0.001; OR=1.062, P=0.001). The area under the curve (AUC) of EC in postmenopausal women was 0.901(95% CI=0.867-0.934) by the combination of the above independent factors and the parameters of P<0.10 (blood flow and NLR), the sensitivity was 78.4% and the specificity was 90.6%. Conclusions:The combination of post-menopausal bleeding, intrauterine fluid, the diameter of the mass, blood flow and NLR have certain clinical application value in predicting EC in postmenopausal women.

Objective:To explore the value of transvaginal ultrasound (TVS) combined with neutrophil to lymphocyte ratio (NLR) in postmenopausal women with endometrial cancer(EC).Methods:A retrospective analysis was conducted on 348 postmenopausal patients who underwent hysteroscopy or surgery in the Obstetrics and Gynecology Hospital of Fudan University from January 2019 to December 2021 and had pathological results of EC, and they were included in the malignant group. One hundred and six benign patients with pathological results in the same period were randomly selected for inclusion in the benign group. The differences in age, menopausal time, gravidity, body mass index (BMI), post-menopausal bleeding, TVS and NLR were compared between the two groups. Binary Logistic regression analysis was performed to determine the independent predictive factors for endometrial cancer in postmenopausal women. ROC curves were plotted to evaluate the diagnostic efficacy of predicting EC in postmenopausal women.Results:Parity (≥2 times), post-menopausal bleeding, intrauterine fluid, the diameter of the mass, blood flow and NLR showed statistically significant differences between the malignant group and the benign group (all P<0.05). Logistic regression analysis showed that post-menopausal bleeding, intrauterine fluid and long diameter of the mass were independent predictors for EC in postmenopausal women( OR=0.433, P=0.006; OR=14.933, P<0.001; OR=1.062, P=0.001). The area under the curve (AUC) of EC in postmenopausal women was 0.901(95% CI=0.867-0.934) by the combination of the above independent factors and the parameters of P<0.10 (blood flow and NLR), the sensitivity was 78.4% and the specificity was 90.6%. Conclusions:The combination of post-menopausal bleeding, intrauterine fluid, the diameter of the mass, blood flow and NLR have certain clinical application value in predicting EC in postmenopausal women.

Objective:To study the molecular mechanism for momordin in inducing apoptosis of multidrug-resistant human chronic leukemia K562/A02 cells. Methods:The growth inhibition value of K562/A02 cells was detected by CCK-8 method. Cell apoptosis was analyzed by Annexin Ⅴ flow cytometry (FCM) and cell morphological examination. FCM was also used in determining expression of P-glycoprotein, p53 protein, bcl-2 protein and caspase activity. Results:Momordin inhibited the proliferation of K562/A02 cells in a dose-dependent manner. It also induced cell apoptosis, reduced the expression of P-glycoprotein, p53 protein and bcl-2 protein, and increased caspase-3 and caspase-8 activity.Conclusion:Momordin reversed the inhibition of apoptosis in multidrug-resistant K562/A02 cells. The molecular mechanism may be related with down-regulation of expression of p53 protein, P-glycoprotein, and bcl-2 protein and up-regulation of caspase-3 and caspase-8 activities.

AIM: To explore the change of the expression of cell adhesion molecule CD_ 54 and CD_ 44 in erythroleukemic K562 apoptosis cells induced by momordin from momordica charantia seeds and study the effects of cell adhesion molecule CD_ 54 and CD_ 44 on cell apoptosis induced by momordin. METHODS: After the treatment of K562 cells with appropriated concentration momordin, CCK-8 test was employed to determine K562 cells growth; flow cytometry FACScan (FITC-Annexin V staining) and electron microscopy were used to detect apoptosis; The expression of CD_ 54 and CD_ 44 were examined by flow cytometry FACScan (FITC-CD_ 54 and PE-CD_ 44 staining). RESULTS: CCK-8 test showed K562 cells growth was significant inhibited by momordin; the apoptosis was detected by cell morphology and flow cytometry FACScan (FITC-Annexin V) in K562 cells after treatment by appropriated concentration momordin. The expressions of CD_ 54 and CD_ 44 in momordin treated K562 cells were 18.62 % and 1.32 % respectively, and in negative momordin treated K562 cells were 0.25 % and 0.17 % respectively, and momordin could up-expresses the protein of CD_ 54 18.37 % and CD_ 44 1.15 %. CONCLUSION: Momordin can markedly induce the K562 cell to apoptosis. The up-expressions of CD_ 54 exist in the process of apoptosis induced by momordin. The change of cell adhesion molecule maybe one of the key factors in the mechanisms of apoptosis induced by momordin, and its mechanism maybe involve in adhesion-dependent apoptosis.