[Objective]To explore the therapeutic effect of Huaier(Trametes robiniophila Murr.) water extracts on granulomatous lobular mastitis(GLM) rat by reducing inflammation levels.[Methods]Female SD rats were randomly divided into normal group,model group,Huaier group[Huaier water extracts 4.5 g/(kg·d)]and hormone group[prednisone acetate 0.0018 g/(kg·d)].The GLM model was induced with tissue homogenate mixture of GLM tissue and Freund's complete adjuvant.After 14 days of administration,the mammary glands of each group were observed;the inflammation index score of the rat mammary gland was evaluated by breast inflammation index score;pathological observation of breast tissue was performed after hematoxylin-eosin(HE) staining and the level of inflammatory factors in the rat serum was detected by enzyme-linked immunosorbent assay(ELISA) method;16S rRNA amplicon sequencing technique was used to determine the diversity and richness of intestinal flora,and the relative abundance of intestinal flora in each group.[Results]Compared with normal group,the rats in model group showed symptoms such as the red breast skin,obvious lumps and significantly increased breast inflammation index score(P<0.05).Pathological changes included the formation of granulomas centered on the breast lobules,with a large number of lymphocytes,plasma cells and other inflammatory cells infiltration,which confirmed the successful induction of GLM rat model.Compared with model group,the breast swelling was improved,and the breast inflammation index score of Huaier group and hormone group both decreased(P<0.05,P<0.05).And the level of inflammatory factors interleukin-6(IL-6),interleukin-17A(IL-17A) and tumor necrosis factor-α(TNF-α) in serum significantly decreased(P<0.05,P<0.05,P<0.05).It could alleviate the infiltration of inflammatory cells in the mammary gland tissue of model group.Significant alterations in the gut microbiota of GLM rat models were observed,with statistically significant differences(P<0.05).After modeling,the abundances of Lactobacillales,Actinobacteria,Bifidobacteriales and Coriobacteriia were downregulated,while those of Caulobacterales and Desulfobacterota were upregulated within the gut microbiota.After the intervention with Huaier water extracts,the abundances of Clostridia were downregulated,while those of Bacteroidetes and Rhodospirillales were upregulated in the gut microbiota of GLM model rats.[Conclusion]Huaier water extracts could alleviate the infiltration of inflammatory cells in the mammary gland tissue of GLM model rats;reduce the proliferation of epithelial cells and the formation of inflammatory granuloma;decrease the level of inflammatory cytokines in the serum of GLM rat model and adjust the intestinal flora of GLM rat models,which may be related to its ability to improve the immunity and relieve the inflammatory response.

[Objective]To explore the therapeutic effect of Huaier(Trametes robiniophila Murr.) water extracts on granulomatous lobular mastitis(GLM) rat by reducing inflammation levels.[Methods]Female SD rats were randomly divided into normal group,model group,Huaier group[Huaier water extracts 4.5 g/(kg·d)]and hormone group[prednisone acetate 0.0018 g/(kg·d)].The GLM model was induced with tissue homogenate mixture of GLM tissue and Freund's complete adjuvant.After 14 days of administration,the mammary glands of each group were observed;the inflammation index score of the rat mammary gland was evaluated by breast inflammation index score;pathological observation of breast tissue was performed after hematoxylin-eosin(HE) staining and the level of inflammatory factors in the rat serum was detected by enzyme-linked immunosorbent assay(ELISA) method;16S rRNA amplicon sequencing technique was used to determine the diversity and richness of intestinal flora,and the relative abundance of intestinal flora in each group.[Results]Compared with normal group,the rats in model group showed symptoms such as the red breast skin,obvious lumps and significantly increased breast inflammation index score(P<0.05).Pathological changes included the formation of granulomas centered on the breast lobules,with a large number of lymphocytes,plasma cells and other inflammatory cells infiltration,which confirmed the successful induction of GLM rat model.Compared with model group,the breast swelling was improved,and the breast inflammation index score of Huaier group and hormone group both decreased(P<0.05,P<0.05).And the level of inflammatory factors interleukin-6(IL-6),interleukin-17A(IL-17A) and tumor necrosis factor-α(TNF-α) in serum significantly decreased(P<0.05,P<0.05,P<0.05).It could alleviate the infiltration of inflammatory cells in the mammary gland tissue of model group.Significant alterations in the gut microbiota of GLM rat models were observed,with statistically significant differences(P<0.05).After modeling,the abundances of Lactobacillales,Actinobacteria,Bifidobacteriales and Coriobacteriia were downregulated,while those of Caulobacterales and Desulfobacterota were upregulated within the gut microbiota.After the intervention with Huaier water extracts,the abundances of Clostridia were downregulated,while those of Bacteroidetes and Rhodospirillales were upregulated in the gut microbiota of GLM model rats.[Conclusion]Huaier water extracts could alleviate the infiltration of inflammatory cells in the mammary gland tissue of GLM model rats;reduce the proliferation of epithelial cells and the formation of inflammatory granuloma;decrease the level of inflammatory cytokines in the serum of GLM rat model and adjust the intestinal flora of GLM rat models,which may be related to its ability to improve the immunity and relieve the inflammatory response.

Objective To explore the strategies of using psychological crisis intervention in public emergencies.Methods In the early stage,psychological crisis intervention concepts and behavioral responses were formed through literature research and qualitative research methods.Use three rounds of Delphi for expert advice.Three indexes including positive coefficient,authority and coordination were used to test the reliability of expert consultation.Results Overall,it passed 239 out of 269 entries.The first round passed item 210,modified item 18,no consensus item 41;In the second round,8 new entries were added,and 22 entries were deleted through 24 entries.In the third round,there were 5 entries and 16 entries were deleted.The authority coefficient was 0.859,indicating that the experts were very familiar with the items.The coordination coefficient of three rounds of consultations were 0.464,0.696,and 0.407 (P＜0.001)respectively,showing that the coordination degree of experts was excellent,the credibility of feedback was high,and results were highly reliable.Conclusions The strategies of field psychological crisis intervention for public emergencies developed in this study received the consensus by domestic psychological crisis intervention experts,it is will provide a basis for systematic,comprehensive and effective guidance for this work.

Objective To evaluate the value of PLD associated with O 3 injection for the treatment of lumbar disc heniation.Methods Lateral decubitus position on healthy side under the direction of DSA, a working pipe was inserted from the back lateral way to damage the disc contents through multiple angles followed by extirpation of the nucleus pulposus and then injecting O 2-O 3 10 ml into the lumbar disc space and near by area of the inter vertebral foramina. As for patients with L4-5 and L5-S1 protrusions the main lumbar discs can be treated in the same way, but for another minor one, the injection of O 2-O 3 10 ml into the lumbar disc and the nearby intervertebral foramina and ethanol through a 21G needle would be properly promising. Results Puncture and technical opertaion were performed successfully in all the patients with excellent results in 63, good in 27, and poor in 10. 120 cases with injection of O 2-O 3 into the lumbar discs showed the figure of lumbar space displaying clear in 37 cases, less clear in 35 cases, only gas entering lumbar disc at the needling point with lumbar space displaying unclear in 30 cases and lots of gas entering the front space of canalis vertebralis cavitas epiduralis in 18 cases. Conclusions PLD associated with O 3 injection is the advantageous supplement mutually posscessing safety and efficancy.