Objective To investigate the diagnostic value of optic nerve damage in glaucoma patients caused by abnormal expression of(Klotho)and C-C chemokine receptor type 7(CXCR7).Methods A prospective study was conducted,totally 120 glaucoma patients treated in the hospital from January 2020 to December 2023 were selected as the study group,and 120 volunteers who underwent physical examination during the same period were selected as the control group.The expression levels of Klotho and CXCR7 in patients with glaucoma caused by abnormal expression of Klotho and CXCR7 were compared between the study group and the control group,the group with or without nerve function injury,and the patients with different degrees of nerve function injury,respectively,and the diagnostic value of optic nerve degree damage in glaucoma patients caused by abnormal expression of KlothO and CXCR7 was studied.Results Klotho and CXCR7 in study group were significantly higher than those in control group(t=93.175,33.855,both P＜0.001).Klotho and CXCR7 in patients with optic nerve damage was significantly higher than that in patients without optic nerve damage(t=5.619,6.507,both P＜0.001).There were statistically significant differences in the expression of Klotho and CXCR7 among patients with different degrees of optic nerve damage(F=12.332,11.209,both P＜0.001).The results of multivariate Logistic analysis showed that both Klotho(OR=3.353)and CXCR7(OR=3.102)were risk factors causing optic nerve damage in glaucoma patients(P＜0.05).According to re-ceiver operating characteristic(ROC)curve analysis,the combined detection of Klotho and CXCR7 showed significantly larger area under the curve for diagnosing glaucomatous optic nerve damage than that of single detection.Conclusion The abnormal expression of Klotho and CXCR7 could be used as an important diagnos-tic basis for the degree of optic nerve damage in glaucoma patients.