Objective To analyze the relationship of silent information regulator 2(SIRT2)with the occurrence and severity of cognitive impairment in patients with acute cerebral infarction(ACI).Methods Clinical data of 150 ACI patients(study group)complicated with cognitive im-pairment admitted in our hospital from May 2022 to May 2024 were collected and analyzed retro-spectively.According to the severity of cognitive impairment,they were divided into mild(45 cases),moderate(66 cases)and severe subgroups(39 cases).Another 125 ACI patients without cognitive dysfunction admitted in our hospital during the same period were recruited and served as the control group.Spearman correlation analysis was adopted to analyze the correlation between serum SIRT2 level and cognitive impairment and disease severity.ROC curve analysis was applied to assess the value of serum SIRT2 level for cognitive impairment and its severity,and its area under the curve(AUC)was calculated.Results The serum SIRT2 level was significantly higher in the study group than the control group(20.38±5.19 mg/L vs 14.66±4.49 mg/L,P＜0.05),so was in the severe subgroup than the moderate and mild subgroups,and in the moderate subgroup than the mild subgroup(P＜0.05).Spearman analysis showed that serum SIRT2 level was posi-tively correlated with the occurrence and severity of cognitive impairment(r=0.510,r=0.527,P＜0.01).ROC curve analysis indicated that the AUC value of serum SIRT2 level in evaluating the occurrence of cognitive impairment was 0.796(95％CI:0.743-0.842),with a cutoff value,sensitivity and specificity of 19.0 mg/L,63.33％and 84.00％,respectively,and the AUC value was 0.747(95％CI:0.655-0.824)in assessing the severity of cognitive impairment,with a cutoff value,sensitivity and specificity of 17.2 mg/L,75.76％and 71.11％,respectively(P＜0.05).Conclusion Serum SIRT2 level is closely associated with the occurrence and severity of cognitive impairment in ACI patients.

Objective To analyze the relationship of silent information regulator 2(SIRT2)with the occurrence and severity of cognitive impairment in patients with acute cerebral infarction(ACI).Methods Clinical data of 150 ACI patients(study group)complicated with cognitive im-pairment admitted in our hospital from May 2022 to May 2024 were collected and analyzed retro-spectively.According to the severity of cognitive impairment,they were divided into mild(45 cases),moderate(66 cases)and severe subgroups(39 cases).Another 125 ACI patients without cognitive dysfunction admitted in our hospital during the same period were recruited and served as the control group.Spearman correlation analysis was adopted to analyze the correlation between serum SIRT2 level and cognitive impairment and disease severity.ROC curve analysis was applied to assess the value of serum SIRT2 level for cognitive impairment and its severity,and its area under the curve(AUC)was calculated.Results The serum SIRT2 level was significantly higher in the study group than the control group(20.38±5.19 mg/L vs 14.66±4.49 mg/L,P＜0.05),so was in the severe subgroup than the moderate and mild subgroups,and in the moderate subgroup than the mild subgroup(P＜0.05).Spearman analysis showed that serum SIRT2 level was posi-tively correlated with the occurrence and severity of cognitive impairment(r=0.510,r=0.527,P＜0.01).ROC curve analysis indicated that the AUC value of serum SIRT2 level in evaluating the occurrence of cognitive impairment was 0.796(95％CI:0.743-0.842),with a cutoff value,sensitivity and specificity of 19.0 mg/L,63.33％and 84.00％,respectively,and the AUC value was 0.747(95％CI:0.655-0.824)in assessing the severity of cognitive impairment,with a cutoff value,sensitivity and specificity of 17.2 mg/L,75.76％and 71.11％,respectively(P＜0.05).Conclusion Serum SIRT2 level is closely associated with the occurrence and severity of cognitive impairment in ACI patients.

Objective: To investigate the antitumor activities and apoptosis mechanisms of 3,7-dinitrodibenzobromonium salt (cBr) on human chronic myelogenous leukemia K562 cell line in vitro. Methods: The antitumor activities of cbr were tested by Trypanblau exclusion test, MTF coloretric assay and clonal forming test, and the mechanisms were studied by morphological analysis (microscopy, fluoreseencemicroscopy, electron microscopy) and flow cytometry analysis. Resuits: cBr could inhibit of the proliferation K562 cells and kill them significantly. The effects varied with different time and doses, and IC50 of cell colony forming rate was 0. 497 mmoL/L. Apoptosis rate tested by flow cytometry was 70.34%,and apoptotic characteristics could be found by microscopy and electron microscopy. Conclusion: cBr could inhibit K562 cell proliferation greatly, and could induce K562 cells into apoptosis.