Objective:To compare the modeling effects of anhydrous ethanol chemical injury method and uterine curettage combine uterine lipopolysaccharide indwelling double injury method, and to explore the stability of the models.Methods:Totally 110 SD female rats were randomly divided into normal group ( n=30), dual injury group ( n=40) and alcohol group ( n=40) by using a random number table. In the alcohol group, 95% absolute ethanol was injected into the uterine cavity to damage the endometrium, and in the dual injury group, lipopolysaccharide cotton thread was retained in the uterine cavity for 2 d after uterine curettage, and sham operation was performed in the normal group. After retrieval of the uteri on the 1st, 2nd, 4th, 8th and 12th weeks post-procedure, they were embedded in paraffin, serially sectioned at 5 μm thickness and routinely stained with hematoxylin and eosin and masson stains. The sections obtained from the samples collected from the above were also used to detect the expression of CD31 (a vascular marker) on the uterine tissues by immunohistochemistry staining. Results:1) Two weeks after operation, the endometrial thickness of the two models were smaller than that of the normal group, and tended to be stable. The thickness of endometrium in alcohol group decreased more significantly than that in dual injury group (all P<0.05). 2) One week post-procedure, the number of endometrial glands in the two models was significantly less than that in the normal group, and it was more obvious in the alcohol group than that in the dual injury group (all P<0.05). 3) The area of endometrial interstitial fibrosis in the model groups were both higher than that in the normal group from one week after operation (all P<0.05), but the fibrosis area in the alcohol group decreased from the 8th week. 4) One week post-procedure, the expression of CD31 in the endometrial stroma of the two models were significantly lower than that in the normal group ( P<0.05), and the decrease in the alcohol group was more obvious than that in the dual injury group (all P<0.05). 5) The comparison of the pregnancy ability and the number of fetal mice between 2-week and 12-week model mice showed that the fertility of the model mice was affected, and the fertility of the alcohol group was more affected than that of the dual injury model group. Conclusion:The two methods can both induce uterine adhesion. Alcohol injury method is simple and economical, but the stability of fibrosis is poor, and the thickness of endometrium, the number of glands and the changes of blood vessels accord with the pathological changes of thin endometrium, which is more suitable for the study of thin endometrial diseases. The dual injury model is close to the clinical injury method, and the model is more stable, which can meet the needs of uterine adhesion related research.

Objective:To compare the modeling effects of anhydrous ethanol chemical injury method and uterine curettage combine uterine lipopolysaccharide indwelling double injury method, and to explore the stability of the models.Methods:Totally 110 SD female rats were randomly divided into normal group ( n=30), dual injury group ( n=40) and alcohol group ( n=40) by using a random number table. In the alcohol group, 95% absolute ethanol was injected into the uterine cavity to damage the endometrium, and in the dual injury group, lipopolysaccharide cotton thread was retained in the uterine cavity for 2 d after uterine curettage, and sham operation was performed in the normal group. After retrieval of the uteri on the 1st, 2nd, 4th, 8th and 12th weeks post-procedure, they were embedded in paraffin, serially sectioned at 5 μm thickness and routinely stained with hematoxylin and eosin and masson stains. The sections obtained from the samples collected from the above were also used to detect the expression of CD31 (a vascular marker) on the uterine tissues by immunohistochemistry staining. Results:1) Two weeks after operation, the endometrial thickness of the two models were smaller than that of the normal group, and tended to be stable. The thickness of endometrium in alcohol group decreased more significantly than that in dual injury group (all P<0.05). 2) One week post-procedure, the number of endometrial glands in the two models was significantly less than that in the normal group, and it was more obvious in the alcohol group than that in the dual injury group (all P<0.05). 3) The area of endometrial interstitial fibrosis in the model groups were both higher than that in the normal group from one week after operation (all P<0.05), but the fibrosis area in the alcohol group decreased from the 8th week. 4) One week post-procedure, the expression of CD31 in the endometrial stroma of the two models were significantly lower than that in the normal group ( P<0.05), and the decrease in the alcohol group was more obvious than that in the dual injury group (all P<0.05). 5) The comparison of the pregnancy ability and the number of fetal mice between 2-week and 12-week model mice showed that the fertility of the model mice was affected, and the fertility of the alcohol group was more affected than that of the dual injury model group. Conclusion:The two methods can both induce uterine adhesion. Alcohol injury method is simple and economical, but the stability of fibrosis is poor, and the thickness of endometrium, the number of glands and the changes of blood vessels accord with the pathological changes of thin endometrium, which is more suitable for the study of thin endometrial diseases. The dual injury model is close to the clinical injury method, and the model is more stable, which can meet the needs of uterine adhesion related research.