Objective: To explore the relationship between pulmonary function, physical activity, and health-related quality of life (QoL) in resected lung cancer patients based on the International Classification of Functioning, Disability, and Health (ICF) framework developed by the World Health Organization to describe health and health-related states. Methods: A quantitative study was designed with postoperative lung cancer survivors to assess personal characteristics. We also assessed functional impairment related to the lung using forced vital capacity (FVC) and forced expiratory volume at 1 second (FEV1), activity limitations using maximal oxygen consumption (VO2max), anaerobic threshold (AT) and 6-minute walking distance (6MWD), and participation restriction using the 36-item Short Form Health Survey V1 (SF-36). Data analyses were conducted using the multivariate method and Smart- PLS to examine path coefficient among the measures. Results: Forty-one patients were enrolled in this study. FVC and FEV1 were poorly correlated with QoL, and 6MWD, AT, or VO2max were positively associated with QoL. AT or VO2max showed a significant (p<0.01) direct path with SF-36 in the ICF model. Although age and body mass index were not strongly correlated with QoL, these personal factors had a medium to large effect on perceived QoL. Conclusion Disability is a complex in patients with lung resection, and physical activity plays an important role in enabling participation. Improving VO2max and AT is needed to improve the QoL of resected lung cancer patients. We should also pay more attention to contextual factors that have a significant impact on social participation.

Objective:This study aims to study the mental health status of people with different identities during the fight against COVID-19. Further, we propose psychological intervention strategies for people with different identities.Methods:857 participants from Guangdong province (215 identified as first-level population referring to the impact severity of the outbreak, 91 as second-level population, 120 as third-level population, and 431 as fourth level population) were cross-sectionally surveyed online from January 30 to February 28, 2020. The Generalized Anxiety Disorder Scale (GAD-7), the Patient Health Questionnaire-9 (PHQ-9), and the Perceived Stress Scale (PSS-10) were used to evaluate the mental health status of different populations. The Kruskal-Wallis H test was used to test the differences of the total scores of these three scales across populations at different levels, and the groups were compared pairwise with the Bonferroni method. Finally, the Wilcoxon rank sum test was used to further analyze the dimensions between the scales. Results:In all 857 subjects, the total scores of GAD-7, PHQ-9, and PSS-10 were 4(1,8), 4(1,9), and 16(11,19), respectively. There were statistically significant differences in the scores of GAD-7 ( H=15.235, P<0.01), PHQ-9 ( H=9.265, P=0.026), and PSS-10 ( H=8.435, P=0.049) among different levels of the population. For the score of GAD-7, the anxiety severity of the first-level crowd was higher than that of the second-level crowd and the fourth-level crowd. The pairwise comparisons were statistically significant ( Z=-2.932, -4.012, -2.949, P<0.005); there were significant between-group differences in domains of "becoming easily annoyed or irritable" and "seemingly terrible things will happen". For the score of PHQ-9, the depression severity of the first-level crowd and the second-level crowd was higher than that of the fourth-level crowd, and the pairwise comparisons were statistically significant ( Z=-3.387, -2.682, P<0.005). There were significant between-group differences in domains of "difficult to fall asleep or not awake", "feeling depressed" and other related domains. For the score of PSS-10, the stress severity of the third-level crowd was higher than that of the first-level crowd and the fourth-level crowd ( Z=-2.702, -3.693, -2.735, P<0.005). There were significant between-group differences in domains of "feel confident", "life is as expected", "the ability to control anger", "the things are all under control", and other related domains. Conclusion:The impact of the COVID-19 on mental health in people with different identities is different, and appropriate psychological interventions should be provided for different populations according to their mental health status.

Objective:This study aims to study the mental health status of people with different identities during the fight against COVID-19. Further, we propose psychological intervention strategies for people with different identities.Methods:857 participants from Guangdong province (215 identified as first-level population referring to the impact severity of the outbreak, 91 as second-level population, 120 as third-level population, and 431 as fourth level population) were cross-sectionally surveyed online from January 30 to February 28, 2020. The Generalized Anxiety Disorder Scale (GAD-7), the Patient Health Questionnaire-9 (PHQ-9), and the Perceived Stress Scale (PSS-10) were used to evaluate the mental health status of different populations. The Kruskal-Wallis H test was used to test the differences of the total scores of these three scales across populations at different levels, and the groups were compared pairwise with the Bonferroni method. Finally, the Wilcoxon rank sum test was used to further analyze the dimensions between the scales. Results:In all 857 subjects, the total scores of GAD-7, PHQ-9, and PSS-10 were 4(1,8), 4(1,9), and 16(11,19), respectively. There were statistically significant differences in the scores of GAD-7 ( H=15.235, P<0.01), PHQ-9 ( H=9.265, P=0.026), and PSS-10 ( H=8.435, P=0.049) among different levels of the population. For the score of GAD-7, the anxiety severity of the first-level crowd was higher than that of the second-level crowd and the fourth-level crowd. The pairwise comparisons were statistically significant ( Z=-2.932, -4.012, -2.949, P<0.005); there were significant between-group differences in domains of "becoming easily annoyed or irritable" and "seemingly terrible things will happen". For the score of PHQ-9, the depression severity of the first-level crowd and the second-level crowd was higher than that of the fourth-level crowd, and the pairwise comparisons were statistically significant ( Z=-3.387, -2.682, P<0.005). There were significant between-group differences in domains of "difficult to fall asleep or not awake", "feeling depressed" and other related domains. For the score of PSS-10, the stress severity of the third-level crowd was higher than that of the first-level crowd and the fourth-level crowd ( Z=-2.702, -3.693, -2.735, P<0.005). There were significant between-group differences in domains of "feel confident", "life is as expected", "the ability to control anger", "the things are all under control", and other related domains. Conclusion:The impact of the COVID-19 on mental health in people with different identities is different, and appropriate psychological interventions should be provided for different populations according to their mental health status.

Objective To observe the clinical efficacy of acupuncture-moxibustion plusMEI Te-lan’s manipulations in treating lumbar intervertebral disc herniation (LIDH) due to blood stagnation.Method Totally 248 LIDH patients due to blood stagnation were randomized into a treatment group of 123 cases and a control group of 125 cases. The treatment group was intervened by acupuncture-moxibustion plusMEI Te-lan’s manipulations, while the control group was by acupuncture-moxibustion plus tuina at lower back. The short-form McGill Pain Questionnaire (SF-MPQ) and Oswestry disability index (ODI) were observed before and after intervention, and the clinical efficacies were compared between the two groups.Result The total effective rate and recovery-markedly effective rate were respectively 80.5% and 52.0% in the treatment group, versus 72.8% and 20.0% in the control group. There was a significant difference in comparing the recovery-markedly effective rate between the two groups (P<0.05). In the treatment group, the Pain Rating Index (PRI) score, Visual Analogue Scale (VAS) score, Present Pain Intensity (PPI) score, and ODI score were significantly changed after 3-day, 6-day, and 20-day treatment compared to the scores before treatment (P<0.05). After 20-day treatment, the PRI, VAS, PPI, and ODI scores of the treatment group were significantly different from that of the control group (P<0.05).Conclusion Acupuncture-moxibustion plusMEI Te-lan’s manipulations is an effective method in treating LIDH due to blood stagnation.

Objective To investigate a new single nucleotid polymorphism (SNP) intron5(+4668C/T) in SLC22A12 in primary gout patients and the association between clinical characteristics and genotypes. Methods One hundred and one primary gout patients and 186 healthy subjects were recruited into this study. Blood pressure, body mass index (BMI) was recorded. Serum uric acid, glucose, lipid and creatinine were detected. DNA was extracted from peripheral blood to amplify the fragment located in intron 5. The genotypes of SLC22A12 can be detected with high-resolution melting (HRM) assay, followed by sequencing analysis. Chi-square test was used for statistical analysis. Results ① A new SNP in intron 5 of SLC22A12 was identi-fied successfully by HRM, which was defined as intron 5 (+4668C/T). CC, CT and TT genotypes were unam-biguously distinguished with HRM technology, which was fully concordant with sequencing. ②The genotypes of CC, CT and TT in male and female groups were 28.1%, 33.7%, 38.2% and 20.0%, 47.1%, 32.9%, respectively.③ However, no significant differences of genotype distribution were found concerning BMI, blood pressure, creatinine, total cholesterol and triglyceride in both male group and female group. But the serum uric acid levels in the CC genotype were significantly higher than those with the CT+TT genotypes. ④ The genotype frequencies of CC and CT+TT in high uric acid group were remarkably different from those in low uric acid group (21.2%, 78.8%,; 35.0%, 65.0%; P<0.05). Conclusion A new SNP has been successfully discovered with HRM technology with simplicity, rapidity and accuracy. T allele of intron 5 (+4668C/T) may be a genetic protective factor for hyperuricemia among Chinese population.

Objective To establish real-time PCR method for the detection of JAK2 V617F mutation and evaluate its clinical significance in patients with myeloprollferative disorders and leukemia.Methods 71 chronic myelocytic leukemia(CML) patients, 22 essential thrombocythemia (ET) patients, 11 primary myelofibrosis (PMF) patients, 9 polycythemia vera (PV) patients and 7 cosinophilia patients were enrolled in this study. JAK2 V617F mutation was determined by real-time PCR and amplification refractory mutation system (ARMS), followed by sequencing. Human erythroleukemia cell (HEL cell)DNA was used as homozygous control of JAK2 V617F mutation. The detection limit for either real-time PCR or ARMS was evaluated. Results Real-time PCR assay showed that there was a melting temperature(Tin) peak at (75.0±0.2)℃ for wild type samples and a Tm peak at (76.6±0.2)℃ for mutation type samples. JAK2 V617F mutation was detected in 8(88.9%) patients with PV, 12(54.5%) patients with ET and 7(63.6%) patients with PMF respectively. But there was only one positive case in 71 CML patient (1.4%). The results showed complete concordance with ARMS results and confirmed by sequencing. The mutation could be detected in 102 HEL cells per 106 white blood cells by real-time PCR, whereas the mutation can be assessed in 104 HEL cells per 106 white blood cells by ARMS. Thus, the sensitivity of real-time PCR was 100-fold higher than ARMS. Conclusions The real-time PCR method is successfully established for detection of JAK2 V617F mutation. This method is more sensitive, convenient than ARMS, and suitable for clinical application. There is high frequency of JAK2 V617F mutation in myeloproliferative disorders and it could be used as the diagnostic marker for myeloproliferative disorders.