Cerebral ischemic stroke(CIS)is a serious health hazard with a high disability rate.Its pathogenesis is complex and not yet fully understood,and new effective treatments need to be explored.The newly discovered pyroptosis,which is regarded as a programmed cell death mode of inflammatory response,is involved in the pathogenesis of CIS.Inhibiting cellular scorch death during CIS and reducing the inflammatory response can effectively alleviate CIS brain injury.According to traditional Chinese medicine,defi-ciency of Qi and stasis of blood are the fundamental pathogenesis of CIS.The method of benefiting Qi and activating blood is the main principle for treating CIS.Therefore,in this paper,we link cell pyroptosis with Qi deficiency and blood stasis,review the progress of research on reducing brain tissue damage in CIS by inhibiting pyroptosis through the method of benefiting Qi and invigorating blood.

Cerebral ischemic stroke(CIS)is a serious health hazard with a high disability rate.Its pathogenesis is complex and not yet fully understood,and new effective treatments need to be explored.The newly discovered pyroptosis,which is regarded as a programmed cell death mode of inflammatory response,is involved in the pathogenesis of CIS.Inhibiting cellular scorch death during CIS and reducing the inflammatory response can effectively alleviate CIS brain injury.According to traditional Chinese medicine,defi-ciency of Qi and stasis of blood are the fundamental pathogenesis of CIS.The method of benefiting Qi and activating blood is the main principle for treating CIS.Therefore,in this paper,we link cell pyroptosis with Qi deficiency and blood stasis,review the progress of research on reducing brain tissue damage in CIS by inhibiting pyroptosis through the method of benefiting Qi and invigorating blood.

Animals ; Mice ; Motor Neurons/physiology* ; Spinal Cord

Oxidative stress is the imbalance between oxidation and anti-oxidation, and reactive oxygen species participate in the physiological and pathological process of reproductive system. Nuclear factor erythroid 2 related factor 2/antioxidant response element (Nrf2/ARE) is an important antioxidant stress pathway. Nrf2 can be activated into the nucleus by various factors such as reactive oxygen species and antioxidants, and combine with small Maf protein and ARE to regulate the expression of various antioxidant enzymes, thus playing an antioxidant role. Studies have shown that the Nrf2/ARE antioxidant pathway plays an important regulatory role in reproductive system diseases. In this paper, the research progress of Nrf2/ARE signaling pathway in reproductive system at home and abroad in recent years is reviewed, and the existing problems and future research directions are discussed and prospected.

Oxidative stress is the imbalance between oxidation and anti-oxidation, and reactive oxygen species participate in the physiological and pathological process of reproductive system. Nuclear factor erythroid 2 related factor 2/antioxidant response element (Nrf2/ARE) is an important antioxidant stress pathway. Nrf2 can be activated into the nucleus by various factors such as reactive oxygen species and antioxidants, and combine with small Maf protein and ARE to regulate the expression of various antioxidant enzymes, thus playing an antioxidant role. Studies have shown that the Nrf2/ARE antioxidant pathway plays an important regulatory role in reproductive system diseases. In this paper, the research progress of Nrf2/ARE signaling pathway in reproductive system at home and abroad in recent years is reviewed, and the existing problems and future research directions are discussed and prospected.

Aim This paper aims at investigating the effect of dofetilide on Na+/Ca 2+exchange in ventricular myocytes of adult guinea pigs. Methods Rapid enzymatic isolation procedure was performed to get single ventricular myocytes from adult guinea pigs. After the whole cell configuration was formed, a ramp voltage-clamp pulse depolarized from a holding potential of -40 mV to +60 mV, then hyperpolarized to -120 mV at a rate of 90 mV/s to record the Na+/Ca 2+ exchange current (I Na/Ca) and the influence of dofetilide from 0.03～1.0 ?mol?L -1. Result Dofetilide from 0.03～1.0 ?mol?L -1 concentration-dependently increased the I Na/Ca in both outward and inward mode. The EC 50 of dofetilide on I Na/Ca in outward and inward mode of NCE was 0.178 ?mol?L -1 (95% confidence interval was 0.040～0.787 ?mol?L -1) and 0.178 ?mol?L -1 (95% confidence interval was 0.038～0.842 ?mol?L -1), respectively. Conclusion Dofetilide concentration-dependently increased the outward and inward Na+/Ca 2+ exchange current in ventricular myocytes of adult guinea pigs.

Aim This study aimed at investigating the postconditioning protective effect of KB-R7943 and the proper administration time on cardiac ischemia-reperfusion injury in isolated rat heart. Methods Rat heart was isolated quickly when the animal was anesthetized. With Langendorff perfusion, the left anterior descending coronary artery was ligated for 30 min, then released for 120 min in isolated rat heart. Left ventricular function (LVF) and the infarct size of left ventricle were determined. Result Compared with control, KB-R7943 at 1 ?mol?L-1 perfused during the early period of the reperfusion prevented the post-ischemic depression of LVF, decreased the infarct size by 77 %(P