The iridoid glycosides from Veronica anagallis-aquatica L. alleviate heart failure by modulating the gut microbiota and influencing the production of two metabolites with potential antihypertrophic effects, HVA and 2OH-VA.Image 1.

[This corrects the article DOI: 10.1016/j.apsb.2024.03.030.].

Objective To observe the clinical efficacy of Xingnao Kaiqiao(awakening the mind and opening the orifices)Acupuncture combined with Xifeng Huatan Tongluo(extinguishing wind,resolving phlegm,and unblocking collaterals)Decoction in treating posterior circulation ischemic vertigo(PCIV).Methods A total of 90 patients diagnosed with PCIV who were treated in the rehabilitation department wards and outpatient clinics of the First Affiliated Hospital of Hebei University of Chinese Medicine between July 2022 and July 2024 were selected as study subjects.Patients were randomly divided into an observation group and a control group using a random number table,with 45 cases in each group.Both groups received conventional western medical treatment.The control group received Xingnao Kaiqiao Acupuncture therapy,while the observation group received additional Xifeng Huatan Tongluo Decoction based on the control group's treatment.The treatment duration was 2 weeks.After treatment,clinical efficacy was evaluated by observing changes in blood flow velocity of the vertebrobasilar artery,left vertebral artery,and right vertebral artery,as well as serum levels of nitric oxide(NO)and endothelin-1(ET-1)before and after treatment in both groups.Changes in Dizziness Assessment Rating Scale(DARS)scores and Berg Balance Scale(BBS)scores were compared,and safety and adverse reactions were assessed.Results(1)The total effective rate was 91.11％(41/45)in the observation group and 73.33％(33/45)in the control group.The efficacy of the observation group was significantly superior to that of the control group,with a statistically significant difference(P＜0.05).(2)After treatment,blood flow velocity in the basilar artery,left vertebral artery,and right vertebral artery were significantly improved in both groups(P＜0.05),and the improvement in the observation group was significantly greater than that in the control group(P＜0.05).(3)After treatment,serum levels of NO and ET-1 were significantly improved in both groups(P＜0.05),and the improvement in the observation group was significantly greater than that in the control group,the difference being statistically significant(P＜0.05).(4)After treatment,DARS and BBS scores were significantly improved in both groups(P＜0.05),and the improvement in the observation group was significantly greater than that in the control group,the difference being statistically significant(P＜0.05).(5)During treatment,the incidence of adverse reactions was 13.33％(6/45)in the control group and 4.44％(2/45)in the observation group.There was no statistically significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion Xingnao Kaiqiao Acupuncture combined with Xifeng Huatan Tongluo Decoction significantly improves cerebral blood flow velocity,ameliorates endothelial dysfunction,and consequently regulates balance and alleviates vertigo in patients with PCIV,demonstrating notable clinical efficacy and good safety.

As an active hydroxyanthraquinone ingredient, emodin is abundant in Chinese medicine herbs, such as Rheum palmatum, Polygonum cuspidatum and Polygonum multiflorum.Modern pharmacological studies have shown that emodin has a variety of pharmacological activities including anti-tumor, anti-inflammatory and immunoregulatory, antibacterial and anti-viral effects, myocardial protection, neuroprotection, renal protection, bone protection, antifibrosis and so on, which indicate its high medicinal value and broad application prospects.This article aims to summarize the progress in the pharmacological activity and mechanism of action of emodin published in domestic and international journals over the last 5 years and highlight the potential targets and molecular signaling pathways linked with emodin, so as to provide some clues and references for further development and clinical application of emodin.

Objective:To characterize the biological activity of fibroblasts on the surface of titanium alloy sheets with different ridge widths by investigating the effects of ridge widths on the adhesion, proliferation and differentiation of fibroblasts.Methods:Five groups of titanium sheets with ridge widths of 50 μm, 80 μm, 100 μm, 150 μm and 200 μm were prepared, with all the groove depths being 10 μm. The titanium sheets with no ridges were taken as a control group. After fibroblasts were incubated on the sheets, states of their adhesion were observed by scanning electron microscopy (SEM) at different time points. CCK-8 cell proliferation test and immunofluorescence staining were used to observe proliferation and shape of the cells. The effects of ridge widths on adhesion of fibroblasts were evaluated by Vinculin immunofluorescence staining, and the effects of ridge widths on expression of α-smooth muscle actin ( α-SMA) by immunofluorescence. Results:SEM showed that the cells adhered to the ridges on the titanium sheets 48 hours after inoculation. In the groups with smaller ridge widths (from 50 μm to 150 μm), the cells were slender in shape and grew along the ridge direction. CCK-8 indicated that different ridge widths had no significant effect on the proliferation of fibroblasts between the 6 groups ( P>0.05). Immunofluorescence staining showed that the cells arranged in an orderly direction along the ridges; the long axis of the cells in the 50 μm group showed the best consistency with the extending direction of the ridge, with significant differences among the 6 groups ( P<0.05). The Vinculin test found that the secretion of cell adhesion protein was concentrated in the ridge and semi-quantitative analysis showed that the 50 μm group had the most Vinculin secretion, with significant differences among the 6 groups ( P<0.05). The α-SMA test showed that the ridge width had a regulatory effect on the myogenic differentiation of fibroblasts, and the 50 μm group had the strongest expression of α-SMA, with significant differences among the 6 groups ( P<0.05). Conclusions:Modification of ridges on the surface of titanium sheets may affect arrangement, adhesion and myogenic differentiation of fibroblasts. The ridges of 50 μm in width may lead to stronger polarized arrangement of fibroblasts, more secretion of adhesion-related protein and more pronounced myogenic differentiation of fibroblasts.

This paper summarizes the training methods of clinical nursing decision-making ability at home and abroad, analyzes the connotation of clinical nursing decision-making ability and the existing problems in the current training methods and puts forward suggestions, in order to provide valuable reference for nursing managers and educators to train nurses' clinical decision-making ability, and then improve the decision-making ability of nursing staff and improve the quality of nursing.

ObjectiveTo explore the in-depth cognition and experience of nursing procedure in the course of clinical practice in undergraduatenursing interns. MethodsBy purposive sampling, 15 undergraduate nursing interns who were at the late stage of clinical practice in a ClassⅢ Grade A general hospital in Shanghai were selected from March to May in 2018. Semi-structured interview was adopted to interview them and Colaizzi seven-step method was used to analyze the data. ResultsTotally four themes were extracted, namely, the gradual dilution of theoretical knowledge of nursing procedures, the different understanding of the role of nursing procedures, different views on whether nursing procedures are applied in nursing work, and various deficiencies in the application of nursing procedures in nursing work. ConclusionsStrengthening nursing students＇ study of theoretical knowledge of nursing process,clarifying the important role and application of nursing process,encouraging nursing students to apply nursing procedures in a coordinated and standardized manner ,strengthening the holistic concept of nursing students and improving the consistency of nursing students＇ learning and application of nursing process in different departments are the key points for the continuous improvement of clinical intern teaching.

Objective To systematically evaluate the biomechanical recovery of drilled holes in the femur in SD rats.Methods Eighteen female SD rats were randomized into 3 even groups (n =6).Models of 2-mm drilled holes in bilateral femurs were established in groups A and B with 2 holes on each side while no drilling was performed in group C.Samples were harvested in group A at postoperative 4 weeks,in group B at postoperative 8 weeks while at both 4 and 8 weeks in group C.The samples were evaluated in terms of linear elasticity (compression test),viscoelasticity (relaxation and creep tests) and durability (fatigue failure test).Micro-CT scan was performed to measure the bone volume fraction (BV/TV) and bone mineral density (BMD) of new bone.Sirus red staining was performed to measure regeneration of type Ⅰ collagen of new bone.Results The elasticity modulus,maximum load,compression strength and conditional yield limit in groups A were significantly lower than those in group B which were also significantly lower than those in group C (P ＜ 0.05).At 7,200 s,the relaxation (14.56 ±0.69 MPa) and creep variation (11.37％ ± 0.70％) in group A were significantly higher than those in group B (11.06 0.63 MPa and 8.98％ ± 0.40％) which were also significantly higher than those in group C (6.99 ±0.56 MPa and 5.10％ ±0.23％) (P ＜ 0.05).At the constant amplitude loads from 20 N to 200 N,from 20 N to 300 N and from 20 N to 400 N,the recycling numbers in group A (6,044.3 ±879.7,4,093.3 ±628.5 and 1,919.3 ±847.5) were significantly lower than those in group B (10,192.3 ± 1,109.1,6,750.6 ± 818.0 and 3,376.6 ± 671.3) which were also significantly lower than those in group C (28,068.3 ±2,702.6,11,788.3 ± 1,141.6 and 5,296.3 ± 735.0) (P ＜ 0.05).By micro-CT scan,the BVT and BMD in group A were significantly lower than those in group B which were also significantly lower than those in group C (P ＜ 0.05).The sirus red staining showed the type Ⅰ collagen in the bone defect area was completely regenerated in group B.Conclusion Systematic biomechanical measurements may actually detect the characteristics of biomechanical recovery of bone holes in SD rats,enriching the basic research on the bone damage repairing progress.

Objective To prepare biphasic calcium phosphate/polyvinyl alcohol scaffolds by 3D printing at room temperature and explore the effect of 3D scaffolds on in vitro osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs).Methods After biphasic calcium phosphate and polyvinyl alcohol solutions were mixed,the biphasic calcium phosphate/polyvinyl alcohol composite scaffolds were prepared by room temperature 3D printing combined with freeze drying technique.Non-printing scaffolds were prepared by injection molding.The surface microstructure,porosity,elastic modulus and hydrophilicity of the 2 sorts of scaffolds were measured.The cytological experiments were carried out in 3 groups (n =3):printed scaffold group,non-printed scaffold group and blank control group (no scaffold).After the BMSCs were seeded onto the scaffolds for 7 and 14 days,the 3 groups were compared in terms of cellular proliferation,alkaline phosphatase activity and expression levels of osteogenesis-related genes.Results 3D composite scaffolds with controllable pore size and porosity were prepared successfully,with an average porosity of 59.6％ ± 3.6％ and an average elastic modulus of 429.3 ± 54.3 kPa.After culture for 7 and 14 days,the cellular absorbance values in the printed scaffold group (0.987 ± 0.047 and 1.497 ± 0.076) were significantly higher than those in the non-printed scaffold group (0.767 ±0.063 and 1.181 ±0.098) (P ＜ 0.05) which were in turn significantly higher than those in the blank control group (0.532 ±0.046 and 0.895 ± 0.062) (P ＜ 0.05).After culture for 7 and 14 days,the ALP activity and expression levels of osteogenesis-related genes in the printed and non-printed scaffold groups showed no significant between-group differences (P ＞ 0.05),but were significantly higher than those in the blank control group (P ＜ 0.05).Conclusions Tissue-engineered composite biphasic calcium phosphate/polyvinyl alcohol scaffolds with controllable pore size and good connectivity can be prepared by freeze-drying and room temperature 3D printing techniques.Co-culture of the scaffolds and BMSCs in vitro promotes adhesion,proliferation and osteogenic differentiation of the cells.