Objective:To analyze the research status and hotspots in the field of post endoscopic retrograde cholangiopancreatography pancreatitis (PEP) based on the Web of Science database.Methods:The Web of Science database was searched by using the subject term "post-ERCP pancreatitis," with language limited to English and document types limited to "article" and "review." The time span was from January 1, 2000 to December 31, 2023. VOSviewer and CiteSpace software were used to analyze the publication year, country, institution, author, journal source, and keywords of the included literature, and to create corresponding visualization knowledge maps.Results:A total of 1 197 documents were included, with an overall steady upward trend on the annual volume of PEP publications. The top three countries in terms of PEP publication volume were the United States (334 articles, 31.19%), China (205 articles, 19.14%), and Japan (180 articles, 16.81%). The top three countries in terms of citation frequency were the United States (17 088 times), Italy (3 936 times), and Japan (3 615 times). The top three institutions in terms of publication volume were Indiana University (34 articles), Mayo Clinic (32 articles), and the University of Pittsburgh (23 articles). The top three institutions in terms of citation frequency were Indiana University (3 524 times), Mayo Clinic (2 675 times), and the University of Minnesota (1 951 times). Among Chinese institutions, Shanghai Jiao Tong University ranked the 4th in publication volume (22 articles), and Nanchang University ranked the 10th (17 articles), but their citation frequencies were relatively low (Shanghai Jiao Tong University 233 times, Nanchang University 108 times). The author with the highest publication volume in the PEP field was Sherman S (32 articles), and the author with the highest co-citation frequency ranking was Cotton PB (769 times). The top three journals in terms of publication volume were Gastrointestinal Endoscopy, Endoscopy, and World Journal of Gastroenterology. The keyword network map revealed distinct clusters covering themes such as the pathogenesis, risk factors, pharmacological prevention, procedural prevention, and management of PEP. Burst keyword analysis showed that the keyword with the longest emergence time was "Oddi dysfunction" and the keyword with the highest burst strength was "rectal indomethacin".Conclusions:Since the beginning of this century, PEP research has developed rapidly, and China has a certain academic influence on PEP internationally. Research on risk factors and preventive measures remains a research hotspot in the field of PEP.

Objective:To analyze the research status and hotspots in the field of post endoscopic retrograde cholangiopancreatography pancreatitis (PEP) based on the Web of Science database.Methods:The Web of Science database was searched by using the subject term "post-ERCP pancreatitis," with language limited to English and document types limited to "article" and "review." The time span was from January 1, 2000 to December 31, 2023. VOSviewer and CiteSpace software were used to analyze the publication year, country, institution, author, journal source, and keywords of the included literature, and to create corresponding visualization knowledge maps.Results:A total of 1 197 documents were included, with an overall steady upward trend on the annual volume of PEP publications. The top three countries in terms of PEP publication volume were the United States (334 articles, 31.19%), China (205 articles, 19.14%), and Japan (180 articles, 16.81%). The top three countries in terms of citation frequency were the United States (17 088 times), Italy (3 936 times), and Japan (3 615 times). The top three institutions in terms of publication volume were Indiana University (34 articles), Mayo Clinic (32 articles), and the University of Pittsburgh (23 articles). The top three institutions in terms of citation frequency were Indiana University (3 524 times), Mayo Clinic (2 675 times), and the University of Minnesota (1 951 times). Among Chinese institutions, Shanghai Jiao Tong University ranked the 4th in publication volume (22 articles), and Nanchang University ranked the 10th (17 articles), but their citation frequencies were relatively low (Shanghai Jiao Tong University 233 times, Nanchang University 108 times). The author with the highest publication volume in the PEP field was Sherman S (32 articles), and the author with the highest co-citation frequency ranking was Cotton PB (769 times). The top three journals in terms of publication volume were Gastrointestinal Endoscopy, Endoscopy, and World Journal of Gastroenterology. The keyword network map revealed distinct clusters covering themes such as the pathogenesis, risk factors, pharmacological prevention, procedural prevention, and management of PEP. Burst keyword analysis showed that the keyword with the longest emergence time was "Oddi dysfunction" and the keyword with the highest burst strength was "rectal indomethacin".Conclusions:Since the beginning of this century, PEP research has developed rapidly, and China has a certain academic influence on PEP internationally. Research on risk factors and preventive measures remains a research hotspot in the field of PEP.

To monitor genetic variation of porcine reproductive and respiratory syndrome virus (PRRSV),RT-PCR was used to identify a sample suspected of PRRSV infection.A PRRSV named SC-GY strain was obtained,and its Nsp2,ORF5 and ORF3 genes were used for sequence alignment and phylogenetic tree construction.The results showed that SC-GY strain is highly pathogenic PRRSV American variant strains with Nsp2 gene discontinuous deletion of 30 amino acids,ORF3 gene aa17 a serine (S) insert.Comparing to VR2332,CH-1a,JXA1,HUN4,NADC30,HENAN-XINX and SC2012,the Nsp2,ORF5 and ORF3 of SC-GY shared 70.3％-97.9％,82.4％-97.6％ and 83.1％-98.2％ of nucleotide similarity,and 62.3％-96.3％,78.0％-95.7％ and 81.6％-96.5％ of deduced amino acid similarity;and compared to LV they shared only 18.9％,60.8％ and 63.7％ of nucleotide similarity,and 14.0％,54.9％ and 57.2％ of deduced amino acid similarity.The phylogenetic tree revealed that the SC-GY formed independent small branches although it belonged to the same subgroup as highly pathogenic PRRSV strains.The results showed that in high frequency live vaccine immunization of currently PRRSV,the gene of PRRSV epidemic strain is still in constant variation.Vaccination of live PRRSV vaccines should be reduced and surveillance of PRRSV strains should be enhanced.

Twelve compounds were isolated from the ethanol extract of Fructus Gleditsiae Abnormalis by macroporous resin, silica gel, Sephadex LH-20, MCI and ODS column chromatographies. Their structures were identified on the basis of physicochemical properties and spectral data as gleditsioside A(1), gleditsioside B(2), gleditsioside H(3), gleditsioside I(4), gleditsioside J(5), gleditsioside K(6), gleditsia saponins C′(7), tamarixetin-7-O-β-D-glucopyranoside(8), neohesperidin(9), chrysoeirol-7-O-neohesperidoside(10); syringaresinol- O-β-D-glucopyranoside(11), liriodendrin(12). Compounds 8-12 were firstly isolated from this genus.

Twelve compounds were isolated and purified from ethyl acetatefraction of Cynanchum stauntonii by silica gel, Sephadex LH-20 and ODS column chromatography. Their structures were identified by spectral techniques and physicochemical properties as syringaresinol(1), (-)-(7R, 7′R, 7″R, 8S, 8′S, 8″S)-4′, 4″-dihydroxy-3, 3′, 3″, 5-tetramethoxy-7, 9′ ∶7′, 9-diepoxy-4, 8″-oxy-8, 8′-sesquineolignan-7″, 9″-diol(2), prinsepiol(3), 4-hydroxyacetophenone(4), baishouwubenzophenone(5), 2, 4-dihydroxyacetophenone(6), benzoic acid(7), 1, 4-benzenediol(8), 6-O-［E］-sinapoyl-α-D-glucopyranoside(9a), 6-O-［E］-sinapoyl-β-D-glucopyranoside(9b), 1-O-methyl-α-D-cymadropyranoside(10), β-daucosterol(11), and β-sitosterol(12). Compounds 1-3, 5 and 7-11 were firstly isolated from this plant.

Object To study and identify the chemical constituents of Bombyx Batryticatus. Methods The constituents were isolated from the above materials by column chromatography using silica gel and Sephadex LH-20, purified by crystallization, and identified by spectroscopic methods. Results Eight compounds were isolated and identified. They are 6-methoxy-7-O-?-D-(4′-methoxy) glucopyranosyl coumarin (Ⅰ), ergost-6, 22-dien-3?, 5?, 8?-triol (Ⅱ), palmitic acid (Ⅲ), meso-erythritol (Ⅳ), D-mannitol (Ⅴ), uracil (Ⅵ), ?-sitosterol (Ⅶ), and daucosterol (Ⅷ). Conclusion Coumpound Ⅰ is a new coumarin glycoside.