Objective To assess the factors associated with the restoration of spontaneous circulation (ROSC) and 2-year survival prognosis in patients with cardiac arrest (CA) after acute myocardial infarction (AMI),and after ROSC,the effects of various factors on midian survival time and on 2-year survival.Methods In a registry study from January 2005 to January 2015,all consecutive AMI-induced CA patients treated with cardiopulmonary resuscitation (CPR) admitted to our hospital were enrolled.The survivors were followed-up for 2 years.Univariate analysis was applied to evaluate factors associated with rate of ROSC and 2-year survival.Multivariable logistic regression analysis was applied to evaluate statistically significant factors in the univariate analysis.Medians with inter-quartile ranges were used to describe 2-year survival time affected by various factors after ROSC.Kaplan-Meier survival curve analysis was used to evaluate the effect of factors on 2-year survival.Results A total of 254 cases with CA after AMI were enrolled,including 129 cases of ROSC and 71 cases of 2-year survival.Univariate analysis showed age ≥ 70 years,CA occurred during 22:00-8:00,the duration time ofCPR ≥ 15 min and adrenaline dosage ＞ 5 mg were unfavorable predictors of ROSC;while,left ventricular ejection fraction (LVEF) ≥ 40％ before CA,shockable rhythm and percutaneous coronary intervention (PCI) therapy were favorable predictors.Besides,age ≥ 70 years,intubation during CPR,adrenaline dosage ＞ 5 mg and cardiogenic shock were unfavorable predictors of 2-year survival;While,male,normal daily activity before CA and PCI treatment were favorable predictors.Multivariable analysis showed age,the duration of CPR,adrenaline dosage,LVEF before CA,the rhythm during CPR and PCI therapy were independent predictors of ROSC.Age and PCI therapy were independent predictors of 2-year survival.Among patients,the survival time was affected by various factors after ROSC,and the factors with minimum 25％ and small median value were associated with cardiac rupture,cancer,adrenaline dosage ＞ 5 mg and cardiogenic shock.The factor with maximum 25％ value was PCI treatment (216 days).Kaplan-Meier survival analysis suggested that age ≥ 70 years was an unfavorable factor of 2-years survival (Log-rank test,P=0.007);while,PCI treatment was a favorable factor (Log-rank test,P＜0.01).PCI-related prognosis analysis showed that the effectiveness of PCI was related to the timing of PCI,the number of infarctrelated artery and the difference in culprit lesion.Conclusions The age ≥ 70 years was disadvantageous to both ROSC and 2-year survival.PCI treatment was favorable to both ROSC and 2-year survival.

Objective To investigate the inhibitive effect of hematoxylin A on the rejection of corneal allograft in rats following corneal transplantation.Methods Twenty SD rats were used as donors and another 40 Wistar rats were used as receivers,and the SD-Wistar rat model of allogeneic penetrating keratoplasty was established.The animals were randomly divided into 4 groups:i.e.group A treated with artificial tear (or the control group) ; group B treated with 0.1％ prednisolone eye drops (or the 0.1％ prednisolone group) ; group C treated with 0.5％ Hematoxylin A eye drops (or the Hematoxylin A group) and group D treated with 0.1％ prednisolone eye drops plus 0.5％ Hematoxylin A eye drops (or the combined treatment group).Following surgery,rejection index (RI) and survival time of corneal grafts were compared between patients of different groups,and observations were made on the survived grafts by immunofluorescence staining.Results Slit lamp observation of corneal grafts indicated that group A had obvious rejection reaction,followed by group B and C,and group D showed the slightest reaction.The survival time of corneal grafts for group A,B,C and D were (8.00 ± 0.58),(15.80 ± 0.36),(15.20 ± 0.49) and(23.40 ± 0.43) days respectively.Statistical significance could be noted,when group A was compared with group B,C,and D,and statistical difference could also be found,when group D was compared with group B and C (P ＜0.01).Ten,twenty and thirty days after corneal transplantation,corneal graft rejection indexes of group B,C and D were all significantly lower than that of group A,with statistical significance (P ＜ 0.01).Corneal graft rejection index of group D was significantly lower than those of group B and C,also with statistical significance (P ＜ 0.01).No statistical difference could be seen in corneal graft rejection indexes,when comparisons were made between group B and C (P ＜ 0.01).Results of immunofluorescence staining indicated that the levels of IL-2 in corneal grafts for group A,B,Cand D were respectively(15.91 ±0.14),(8.19 ±0.07),(7.96±0.08)and(5.81 ±0.11).Statistical difference could also be noticed,when group B,C and D were compared with group A,and the same results could be obtained,when group D was compared with group B and C,all with statistical significance (P ＜ 0.01).However,no statistical significance could be seen,when comparisons were made between group B and C (P ＞ 0.05).Conclusions Hematoxylin A could produce certain inhibitive effect on corneal graft rejection,its effect was similar to that of prednisolone,and seemed to have a synergistic effect with prednisolone,the mechanism of which might be associated with the inhibition of IL-2 expression.

Objective To investigate the inhibitive effect of hematoxylin A on the rejection of corneal allograft in rats following corneal transplantation.Methods Twenty SD rats were used as donors and another 40 Wistar rats were used as receivers,and the SD-Wistar rat model of allogeneic penetrating keratoplasty was established.The animals were randomly divided into 4 groups:i.e.group A treated with artificial tear (or the control group) ; group B treated with 0.1％ prednisolone eye drops (or the 0.1％ prednisolone group) ; group C treated with 0.5％ Hematoxylin A eye drops (or the Hematoxylin A group) and group D treated with 0.1％ prednisolone eye drops plus 0.5％ Hematoxylin A eye drops (or the combined treatment group).Following surgery,rejection index (RI) and survival time of corneal grafts were compared between patients of different groups,and observations were made on the survived grafts by immunofluorescence staining.Results Slit lamp observation of corneal grafts indicated that group A had obvious rejection reaction,followed by group B and C,and group D showed the slightest reaction.The survival time of corneal grafts for group A,B,C and D were (8.00 ± 0.58),(15.80 ± 0.36),(15.20 ± 0.49) and(23.40 ± 0.43) days respectively.Statistical significance could be noted,when group A was compared with group B,C,and D,and statistical difference could also be found,when group D was compared with group B and C (P ＜0.01).Ten,twenty and thirty days after corneal transplantation,corneal graft rejection indexes of group B,C and D were all significantly lower than that of group A,with statistical significance (P ＜ 0.01).Corneal graft rejection index of group D was significantly lower than those of group B and C,also with statistical significance (P ＜ 0.01).No statistical difference could be seen in corneal graft rejection indexes,when comparisons were made between group B and C (P ＜ 0.01).Results of immunofluorescence staining indicated that the levels of IL-2 in corneal grafts for group A,B,Cand D were respectively(15.91 ±0.14),(8.19 ±0.07),(7.96±0.08)and(5.81 ±0.11).Statistical difference could also be noticed,when group B,C and D were compared with group A,and the same results could be obtained,when group D was compared with group B and C,all with statistical significance (P ＜ 0.01).However,no statistical significance could be seen,when comparisons were made between group B and C (P ＞ 0.05).Conclusions Hematoxylin A could produce certain inhibitive effect on corneal graft rejection,its effect was similar to that of prednisolone,and seemed to have a synergistic effect with prednisolone,the mechanism of which might be associated with the inhibition of IL-2 expression.

AIM: To observe the effects of Salvia miltiorrhiza Bge (SMB) on left ventricular hypertrophy(LVH)in spontaneously hypertensive rats (SHR) and the expression of c-fos. METHODS: 18 SHRs in 8 weeks old were divided into three groups at random. SMB or distilled water(1 g?kg -1?d -1)was injected intraperitoneally to two groups for 10 weeks. Systolic blood pressure (SBP) and left ventricular mass index(LVMI)were measured. HE,VG and immunohistochemical staining combined with computed morphometry were used to evaluated the cardiomyocyte size and diameter, the collagen volume fraction(CVF), perivascular circumferential area (PVCA) and c-fos expression in the left ventricular tissue. RESULTS: Compared with 8-week-old rats, the SBP, LVMI, cardiomyocyte size and diameter, CVF, PCVA, c-fos expression increased markedly in the 18th week of SHRs. The LVH stopped and c-fos expression decreased whereas SBP changed slightly in animals treated with SMB. CONCLUSION: Chronic treatment with SMB can inhibit the development of LVH in SHR, which is probably related to the decease of cardiac c-fos.