Hepatocellular carcinoma （HCC） is a malignant tumor with high incidence and mortality rates worldwide. Recent studies have shown that neutrophil extracellular traps （NETs） play an important role in the development， progression， and immune escape of HCC. NETs are released by neutrophils and mainly consist of DNA， histones， and antimicrobial molecules， and in addition to immune defense， they are also involved in the initiation， metastasis， and thrombosis of HCC. This article elaborates on the formation and regulatory mechanisms of NETs， explores their potential mechanisms in the initiation， metastasis， immune escape， and thrombosis of HCC， and discusses the prospect of NETs as a target for the diagnosis and treatment of HCC， in order to provide new ideas for the precise treatment of HCC in the future and promote the early diagnosis and effective treatment of HCC.

Objective:To explore the safety and reliability of retrosigmoid approach BONEBRIDGE implantation in patients with auricle reconstruction using skin expansion flap.Methods:A retrospective analysis was conducted on 43 congenital aural atresia cases (43 ears) who underwent BONEBRIDGE implantation from September 2019 to January 2023 in Beijing Tongren Hospital. 30 males and 13 females were included in this work. The implantation age was 9-36 years old (median age=10 y/o). All cases underwent auricle reconstruction surgery using the posterior ear flap expansion method, with 36 cases using the single expanded postauricular flap method and 7 cases using two-flap method. BONEBRIDGE implant surgery was performed during the third stage of auricle reconstruction or after all stages. The hearing improvements were evaluated by comparing the changes in pure tone hearing threshold and speech recognition rate of patients before and after BONEBRIDGE implantation. Routine follow-up was conducted to observe the hearing results and complications. SPSS 14.0 software was applied for data statistical analysis.Results:All 43 patients healed well and had no surgical complications when discharge. The average bone conduction hearing threshold after surgery was (8.2±6.6) dBHL, and there was no statistically significant difference compared to the preoperative [(8.1±5.7) dBHL] ( P=0.95). After surgery, the threshold of hearing assistance with power on was significantly lower than that without hearing assistance [(32.8±4.6) dBHL vs (60.5±5.5) dBHL], and the difference was statistically significant ( P<0.001). The speech recognition rate of monosyllable words, disyllabic words and short sentences in quiet environment increased to 72%, 84%, and 98% respectively. The differences were statistically significant ( P<0.001). The speech recognition rate of monosyllabic words, disyllabic words, and short sentences in noise environment was significantly increased by 70%, 80%, and 92% respectively ( P<0.001). After a follow-up of 4 to 47 months (median=24 months), the hearing results were stable and the aesthetic outcomes were satisfying. One patient had delayed hematoma around coil of the implant. After aspiration and compressed dressing for one week, hematoma was not recurrent. Conclusion:For patients after auricle reconstruction using expanded postauricular flap, the preference of retrosigmoid approach is a good choice in terms of safety and reliability of operation, as well as aesthetic appearance.

Objective    To further understand the anatomical characteristics and rules of left upper lingual pulmonary artery. Methods    CT data of 120 patients (82 males, 38 females, median age of 65 years ranging 36-78 years) with pulmonary nodules from December 2018 to August 2020 in our hospital were retrospectively analyzed. The anatomic characteristics of the lingual segment of the upper left lung were analyzed by three-dimensional reconstruction. Results    Mediastinal lingual artery appeared in 34 of the 120 patients, accounting for 28.4%. There were 26 patients of mixed mediastinal/interlobar type, 8 patients of pure mediastinal lingual artery, and 92.3% (24/26) mixed mediastinal/interlobar type blood vessel contained A4b. Fifty-eight (58/120, 48.3%) patients had interlobar type A4+5 type, the rest were two-branches type. And 22 patients of A4 and A5 type accounted for the most two-branches type (22/28, 78.6%). The single-branch type was located at the distal end of A6 in 54 (54/58, 93.1%) patients, originated from the proximal end of A6 in 4 patients, and originated from the basilar artery in 6 patients. The two-branches type was at the distal end of A6 accounting for 50.0% (14/28). Conclusion    Mediastinal lingual artery is more common than expected, accounting for 28.4%, among which mixed lingual artery is more prevalent, usually located in the first pulmonary trunk, and mostly follows the principle of proximity to supply relevant lung tissues. The location of the interlobar branches in the pulmonary trunk can be at the distal or proximal end of A6, care should be taken to avoid damaging adjacent structures.

Objective? To summarize the efficacy,advantages and nursing experience of the new non-invasive auricle appliance in the treatment of congenital auricle deformities. Methods? Earwell, a new non-invasive auricle appliance, was used to correct 177 children with congenital auricle deformity (258 ears). The nursing points were summarized, including guiding parents' expectations scientifically, parents' health guidance and practice, observation and nursing of the appliance, home management through Wechat platform and complication management. Results? Among the 177 cases, 2 cases (2 ears) failed to complete the correction treatment due to poor compliance; 4 cases (single ear) had eczema, 2 cases (2 ears) had skin pressure sores;1 case (2 ears) had periauricular skin allergies, and finally 168 cases (244 ears) were successfully treated. Earwell appliance treatment success rate was 94%,of which 189 cases were excellent and 55 cases were good. Conclusions? Congenital auricle deformities treatment with Earwell auricle appliance is significantly effective.

Objective To study the relationship between heparin-binding protein(HBP) and β2 integrin on the surface of neutrophil in acute lung injury.Methods A total of 30 mice from animal center of Tongji University were divided into control group,CLP group and antibody-treatment group by random number method.Acute lung injury animal model was established by cecal Ligation and puncture in the mice of CLP group and antibody-treatment group.Sham operations were performed on the mice in control group.Mice in antibody-treatment group received anti-CD18 antibody injected via tail vein 30 min before establishing acute lung injury animal model.Twenty-four hours after operation,mice were sacrificed and lung tissue was taken.After HE staining,lung injury were evaluated by Smith score.Lung tissue wet/dry weight ratio,BALF protein level,plasma HBP level and β2 integrin on neutrophil were measured.T test were used to compare the difference among groups.Pearson correlation was used to study the correlation between HBP,integrin and lung injury index.Results Compared with control group,mice in CLP group had higher Smith score(t=10.607,P＜0.01),lung wet/dry ratio(t=3.968,P=0.001),BALF protein level(t=4.331,P＜0.01) and as well as higher plasma HBP(t=3.515,P=0.002) and β2 integrin(t=4.816,P＜0.01) level.After CD18 antibody treatment,anti-treat group mice had lower Smith score (t=2.307,P=0.033),lung wet/dry ratio(t=3.080,P=0.006),BALF protein level(t=2.484,P=0.023) and as well as higher plasma HBP level(t=2.218,P=0.046) than mice in CLP group.Pearson correlation analysis showed HBP had obvious correlation with wet/dry ratio(r=0.527,P=0.017),BALF protein(r=0.508,P=0.022) and as well as β2 integrin(r=0.674,P=0.001).Conclusions Both HBP and β2 integrin involved in the lung injury pathogenesis.HBP level is interrelated with the severity of lung injury.The β2 integrin is associated with the release of HBP by neutrophil.Inhabiting the function of integrin could decrease HBP level and alleviate the severity of lung injury.

Objective To review the effects of mindfulness-based therapy on depression for stroke patients. Methods The researches about the effects of mindfulness-based therapy on depression for stroke patients were recalled from Cochrane Library, PubMed, EMbase, Web of Science, CNKI, WanFang Data and VIP, and analyzed with RevMan 5.3 software. Results Five researches were included, which contained three randomized controlled trials and two clinical controlled trials. Meta-analysis showed that mindfulness-based therapy could decrease the depression score in stroke patients (SMD = -1.05, Z = 3.52, P < 0.001). Conclusion The mindfulness-based therapy could reduce the depression for stroke patients; however, the means of mindfulness need further research.

Objective To evaluate the under-registration rate related to mortality surveillance program in Jinan city,from 2013 to 2014,using the capture-mark-recapture method.Methods Under the stratified cluster random sampling method,samples were stratified,according to urban and rural areas.Two districts and three counties were chosen,with three towns/streets in each county/district and eight villages/communities in each township/street,randomly selected.With the participation of departments as civil affairs,public security,maternal/child institutions and community committees as well as individuals as village doctors and community leaders,a list of deaths from January 1,2013 to December 31,2014 was collected and compared to the National Mortality Surveillance System on the rates related to under-registration.Results A total of 2 903 records on deaths were collected from 2013 to 2014,while 3 113 deaths were reported in the surveillance system of the same period.3 772 (95％ CI:3 741-3 802) deaths were estimated by the capture-mark-recapture method.The two-year total under-registration rate was 17.46％.The under-registration rates in 2013 and 2014 appeared as 19.29％ and 15.57％ (x2 =8.92,P＜0.01),respectively,with the rates in urban and rural areas as 20.91％ and 11.93％ (x2 =47.35,P＜0.01).The rates on male and female were 17.87％ and 16.98％ (x2 =0.48,P＞0.05).The rates of ＜5 years old group and ≥5 years old group were 21.57％ and 17.45％ (x2 =0.03,P＞0.05) respectively.Conclusions Under-registration in the mortality surveillance was noticed in Jinan.The capture-mark-recapture method seemed useful for evaluating the under-registration rate in the mortality surveillance program.It is suggested that the survey on under-registration rate should be carried out on the regular base.The under-registration rate seemed useful in adjusting the mortality rate.

Objective To assess the impact of heat waves on non-accidental deaths,and to investigate the influencing factors for deaths caused by heat waves in Jinan,China.Methods Daily death data and meteorological data for summer days with or without heat waves in Jinan from 2012 to 2014 were collected,and a cross-over analysis was conducted to evaluate the influence of heat waves on non-accidental deaths and deaths caused by other reasons.The univariate and multivariate logistic regression models were used to investigate the influencing factors for deaths caused by heat waves.Results The risks of non-accidental deaths and deaths caused by circulation system diseases during the days with heat waves were 1.82 times (95％ CI:1.47～2.36) and 1.53 times (95％ CI:1.14～2.07) those during the days without heat waves.The multivariate logistic regression analysis showed that old age (≥75 years)(OR=1.184,95％ CI:1.068～1.313),low educational level (OR=1.187,95％ CI:1.064～1.324),and deaths outside hospital (OR=1.105,95％ CI:1.009～1.210) were associated with the high risk of deaths during the days with heat waves.Conclusion Heat waves significantly increase the risk of non-accidental deaths and deaths caused by circulation system diseases in Jinan,and the deaths during the days with heat waves are related to age,educational level,and place of death.

Objective To assess the impact of heat waves on non-accidental deaths,and to investigate the influencing factors for deaths caused by heat waves in Jinan,China.Methods Daily death data and meteorological data for summer days with or without heat waves in Jinan from 2012 to 2014 were collected,and a cross-over analysis was conducted to evaluate the influence of heat waves on non-accidental deaths and deaths caused by other reasons.The univariate and multivariate logistic regression models were used to investigate the influencing factors for deaths caused by heat waves.Results The risks of non-accidental deaths and deaths caused by circulation system diseases during the days with heat waves were 1.82 times (95％ CI:1.47～2.36) and 1.53 times (95％ CI:1.14～2.07) those during the days without heat waves.The multivariate logistic regression analysis showed that old age (≥75 years)(OR=1.184,95％ CI:1.068～1.313),low educational level (OR=1.187,95％ CI:1.064～1.324),and deaths outside hospital (OR=1.105,95％ CI:1.009～1.210) were associated with the high risk of deaths during the days with heat waves.Conclusion Heat waves significantly increase the risk of non-accidental deaths and deaths caused by circulation system diseases in Jinan,and the deaths during the days with heat waves are related to age,educational level,and place of death.

China ; epidemiology ; Humans ; Mortality ; trends