Objective:To assess the efficacy of "three-step" strategy for preparing autologous pedicled nasal mucosal flaps in repairing cerebrospinal fluid（CSF） leaks following transsphenoidal pituitary adenoma surgery. Methods:A retrospective study was conducted on the clinical data of 25 patients who developed CSF leaks after transsphenoidal pituitary adenoma surgery at the First Affiliated Hospital of Kunming Medical University between July 2012 and June 2022. Surgical repair was selected step by step using nasal septal mucosal flap with either the posterior septal artery or septal branch of the sphenopalatine artery as the pedicle, or a pedicled middle turbinate mucosal flap. All patients underwent ≥2-year endoscopic follow-up to assess flap viability and CSF leak recurrence. Results:The median postoperative hospital stay was 4 days. Five patients developed intracranial infections postoperatively. The follow-up period ranged from 2 to 12 years. Nasal endoscopic examinations showed good mucosal flap growth, with no recurrence of CSF leakage in any of the patients. Conclusion:High-flow cerebrospinal fluid（CSF） leaks following pituitary tumor surgery pose significant challenges for clinical repair. Based on intraoperative nasal septal mucosal preservation and the condition of sellar base CSF leakage, the "three-step" strategy for preparing autologous pedicled nasal mucosal flaps-utilizing posterior septal artery, ethmoidal artery-based, or pedicled middle turbinate mucosal flaps sequentially-is a safe and effective repair method.
Humans ; Retrospective Studies ; Pituitary Neoplasms/surgery* ; Surgical Flaps ; Nasal Mucosa/surgery* ; Cerebrospinal Fluid Leak/surgery* ; Adenoma/surgery* ; Postoperative Complications/surgery* ; Male ; Female ; Middle Aged ; Adult ; Aged

Objective To explore the role and mechanism of crocin in pituitary adenoma(PA)through clinical samples and related molecular biology experiments of HP75 cells.Methods From June 2022 to May 2023,16 PA samples were collected from the Second Department of Neurology and Otolaryngology skull base surgery of the First Affiliated Hospital of Kunming Medical University.Three normal control samples were from the human anatomy of the Forensic College of Kunming Medical University.The expression of IRF7 mRNA in clinical samples was detected,and the proliferation,migration,invasion and apoptosis of HP75 cells were detected by knocking down the expression of IRF7;the expression of NF-κB was regulated by IRF7 in HP75 cells,and crocin regulated the growth of PA cells and its regulatory effect on IRF7/NF-κB signaling pathway.Results RT-qPCR and immunohisto-chemistry showed that compared with the normal control group,the expression of IRF7 mRNA in PA was significantly increased(P＜0.001);the expression of IRF7 protein in si-IRF7 group was significantly decreased(P＜0.001);CCK-8,Transwell and flow cytometry results showed that compared with the control group,knockdown of IRF7 significantly decreased the cell viability of HP75 cells(P＜0.001),inhibited the migration and invasion(P＜0.001),and promoted the apoptosis of HP75 cells(P＜0.001).In addition,knockdown of IRF7 could inhibit the expression of p-NF-κB p65/NF-κB p65(P＜0.001)and p-NF-κB p65/NF-κB p65(P＜0.001).Overexpression of IRF7 partially reversed the effect of crocin(P＜0.001)and restored the expression of p-NF-κB p65/NF-κB p65(P＜0.01).Finally,the biological behavior of HP75 cells showed that compared with crocin group,overexpression of IRF7 could improve the cell viability of HP75 cells,promote their migration and invasion,and inhibit cell apoptosis(P＜0.001).Conclusion Crocin treatment can inhibit the proliferation,migration and invasion of PA cells,promote cell apoptosis,and alleviate the development of PA.In the mechanism,IRF7 is significantly overexpressed in PA,and knockdown of IRF7 can inhibit the malignant growth of PA.Crocin can inhibit the proliferation,migration and invasion of PA cells,and promote apoptosis by inhibiting IRF7/NF-κB signaling pathway.

Objective:To evaluate the quality of relevant practice guidelines for the prevention of surgical site infections in adult inpatients and provide a reference for formulating protocols to prevent surgical site infections in adults.Methods:Practice guidelines related to the prevention of surgical site infections in adults published up to June 2, 2020 were retrieved from domestic and foreign databases, guideline websites and the websites of professional societies. The Appraisal of Guidelines for Research and Evaluation Ⅱ (AGREE Ⅱ) was used to evaluate the methodological quality of the included guidelines, and the intra-group correlation coefficient was used to test the consistency between the reviewers.Results:A total of 6 clinical practice guidelines were included, and in terms of the quality, there were 4 grade A guidelines and 2 grade B guidelines. The average standardized scores of AGREE Ⅱ in 6 dimensions were: 87.04% for scope and purpose, 76.62% for stakeholder involvement, 76.56% for rigor of development, 89.58% for clarity of presentations, 81.60% for applicability, and 81.25% for independence.Conclusions:The existing clinical practice guidelines related to the prevention of surgical site infections in adults are relatively comprehensive with a relatively good quality. Nursing staff need to consider the applicability of evidence application, formulate practical infection prevention strategies, and reduce the incidence of surgical site infections in adults.

Objective To observe the influence ofgypenosides on hydrogen sulfide in liver tissue and plasma of rat with type 2 diabetes mellitus and nonalcoholic fatty liver disease.Methods 58 SPF male SD rats,with body mass 220～250 g,were randomly divided into a blank control group (group N,n=7),and a NAFLD and T2DM model group (Group M,n=51).Group N was fed with ordinary diet in the first four weeks,group M was fed with diets of high fat and sugar,injected with 40 mg/kg STZ overnight,and the same diets for the next four weeks.The rat model with T2DM and NAFLD was build.NAFLD and T2DM model group were divided into three groups:a high dose GPS group (JH,n=9) injected with 1 g/kg · d-1 GPS,a low dose GPS group (JL,n=9) injected with 0.5 g/kg · d-1 GPS,and a model group (M,n=9) injected with the same volume of water,and high fat diet at the same time.The treatment period was six weeks,and the experiment period was fourteen weeks.TG,TC,BS,and H2S in the plasma of rat were tested,and H2S in the liver tissue of the rat was tested.Results ①The changes of H2S in plasma:group JH [(4.30±0.43) μmol/L] and JL [(3.83 ±0.47) μmol/L] was lower than group M [(2.67 ± 0.41) μmol/L],there was a significant difference.②The changes of H2S in the liver tissue:group JH [(333.52±37.94) pmol/min/mg/protein] and JL [(275.81 ±36.07)pmol/min/mg/protein] was lower than group M [(237.8± 33.05) pmol/min/mg/protein],there was a significant difference.③BS levels:group JH(10.86±3.46)mmol/L,group JL (14.78±3.39)mmol/L,group M(18.84±4.24) mmol/L,group JH and JL was lower than group M,there was a significant difference (P＜0.01).④The plasma TG level:group N (0.96±0.09) mmol/L,group JH (2.82± 0.66) mmol/L,group JL (1.83± 0.56) mmol/L,group M (3.97 ± 0.64) mmol/L.group JH and JL was lower than group M,there was a significant difference (P＜0.01).Conclusion Gypenoside can reduce the blood sugar,triglycerides,and total cholesterol in rat with with type 2 diabetes mellitus and nonalcohol fatty liver disease.H2S concentrations in plasma and liver tissue of the rats with T2DM and NAFLD were increased by GPS,showing dose dependence.Gypenosides can also improve metabolism of blood glucose and lipid in rats with T2DM and NAFLD.