Objective To screen the core genes related to colon cancer in order to provide a new research direction for molecular di-agnosis,targeted therapy and prognosis evaluation of colon cancer.Methods Colon cancer differentially expressed genes(DEGs)were screened out from the gene expression database(GEO),and functional annotation and pathway enrichment analysis of DEGs were per-formed by gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)analysis in Metascape database.The network of protein interactions(PPI)was built utilizing the String database and Cytoscape software to identify the core genes.Survival analysis of se-lected core genes was performed using Kaplan-Meier plotter database,and expression level of core genes was verified using GEPIA2database.Metascape data was used to conduct KEGG pathway enrichment analysis on the selected core genes,and the signal pathway of core gene enrichment was identified.COL1A1 in the core gene was selected for immunohistochemical verification.Results A total of 468 DEGs were identified,including 162 UDEGs and 306 DDEGs.In the two datasets,there were 194 overlapping genes,of which 54 were up-regulated and 140 were down-regulated.With the help of CytoHubba plugin,the top 10gene nodes in the score were selected and identified as the possible core genes of colon cancer(COL1A1,MMP3,COL1A2,COL11A1,THBS2,BGN,MMP1,COL10A1,SPP1,INHBA).The prognosis and expression levels of these 10genes were analyzed,and the expression levels of the 10 core genes were different in colon cancer tissues and normal colon tissues.All of the 10 core genes were correlated with colon cancer prognosis except MMP1,among which MMP3 was positively correlated and the other 8genes were negatively correlated.KEGG analysis showed that core genes were mainly concentrated in three signaling pathways:cytoskeleton,extracellular matrix(ECM)receptor interaction and pro-tein digestion and absorption in muscle cells.Immunohistochemistry analysis revealed a marked increase in the expression level of COL1A1 within the tumor tissue compared to that in paracancer tissue.Conclusion The core genes COL1A1,MMP3,COL1A2,COL11A1,THBS2,BGN,MMP1,COL10A1,SPP1 and INHBA selected by bioinformation analysis may be involved in the occurrence and development of colon cancer,and may be related to the prognosis of patients with colon cancer.It is anticipated that it will serve as a clinically significant biomarker and a therapeutic target for colon cancer.

Objective To screen the core genes related to colon cancer in order to provide a new research direction for molecular di-agnosis,targeted therapy and prognosis evaluation of colon cancer.Methods Colon cancer differentially expressed genes(DEGs)were screened out from the gene expression database(GEO),and functional annotation and pathway enrichment analysis of DEGs were per-formed by gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)analysis in Metascape database.The network of protein interactions(PPI)was built utilizing the String database and Cytoscape software to identify the core genes.Survival analysis of se-lected core genes was performed using Kaplan-Meier plotter database,and expression level of core genes was verified using GEPIA2database.Metascape data was used to conduct KEGG pathway enrichment analysis on the selected core genes,and the signal pathway of core gene enrichment was identified.COL1A1 in the core gene was selected for immunohistochemical verification.Results A total of 468 DEGs were identified,including 162 UDEGs and 306 DDEGs.In the two datasets,there were 194 overlapping genes,of which 54 were up-regulated and 140 were down-regulated.With the help of CytoHubba plugin,the top 10gene nodes in the score were selected and identified as the possible core genes of colon cancer(COL1A1,MMP3,COL1A2,COL11A1,THBS2,BGN,MMP1,COL10A1,SPP1,INHBA).The prognosis and expression levels of these 10genes were analyzed,and the expression levels of the 10 core genes were different in colon cancer tissues and normal colon tissues.All of the 10 core genes were correlated with colon cancer prognosis except MMP1,among which MMP3 was positively correlated and the other 8genes were negatively correlated.KEGG analysis showed that core genes were mainly concentrated in three signaling pathways:cytoskeleton,extracellular matrix(ECM)receptor interaction and pro-tein digestion and absorption in muscle cells.Immunohistochemistry analysis revealed a marked increase in the expression level of COL1A1 within the tumor tissue compared to that in paracancer tissue.Conclusion The core genes COL1A1,MMP3,COL1A2,COL11A1,THBS2,BGN,MMP1,COL10A1,SPP1 and INHBA selected by bioinformation analysis may be involved in the occurrence and development of colon cancer,and may be related to the prognosis of patients with colon cancer.It is anticipated that it will serve as a clinically significant biomarker and a therapeutic target for colon cancer.

Objective To investigate the expression of serotonin transporter (5-HTT) and serotonin 1A treceptor (5-HT1 A R) located in the chronic unpredictable stress (CUS)-relative brain areas (mPFC,VTA,NAc) in high and low CUS susceptibility rats,thus to unveil the possible mechanism lead to the different CUS susceptibility.Methods One hundred and fifty male Sprague-Dawley rats were randomly assigned into experiment group (n =120) and control group (n =30).Rats in experiment group were trained according to established CUS procedure.OFT and FST were used to assess the different susceptibility to CUS:high susceptibility group (H group)and low susceptibility group (L group).After the model was established,rats were scarified and cardio-perfused,and the brains were removed and sliced up coronarily.The sections including ventral tegmental area (VTA),nucleus accumben (NAc),medial prefrontal cortex (mPFC) were selected.The mRNA levels of 5-HTT and 5-HT1AR in the regions were estimated with in situ hybridization.Results The expression of 5-HTT in H group were significantly lower than that of in the control and L group in all regions (mPFC:169.20 ± 8.23 vs 143.53 ±5.31 ; Nac:177.41 ± 5.68 vs 158.65 ± 5.24 ; VTA:174.16 ± 5.61 vs 158.65 ± 4.85),and the difference between the H and L group was significant(P＜0.01) ;however,the expression of 5-HT1AR in H group were significantly higher than that of in the control and L group in all regions (mPFC:113.98 ± 7.46 vs 125.90 ± 3.30 ; Nac:112.11± 5.50 vs 125.06 ± 3.97 ;VTA:103.11 ± 6.05 vs 115.57 ± 3.19),and the difference between the H and L group was significant (P＜ 0.01).Conclusion The overexpression of 5-HT1AR and down regulation of 5-HTT in the circuit of VTA-NAc-mPFC may be the basis of the high susceptibility to CUS.