Objective To study the effect of direct cover vitrification (DCV ) on the morphology of follicles from human o‐varian tissue .Methods Biopsies of ovarian tissue was cryopreserved by DCV and some of them was cultured for 7 days .The rates of primordial follicles ,primary follicles and secondary follicles were counted .Results Compared to the fresh group and frozen group ,the percent of primordial follicle of fresh‐cultured group ,frozen group decreased ,primary follicle significantly increased(P<0 .05) .The percent of primordial follicle ,primary follicle ,secondary follicles of fresh‐cultured group were lower than those of fresh group(P<0 .01) .In frozen group ,the rates of the primary and secondary follicles with normal morphology were lower than those of fresh cultured group(P<0 .05) .The rate of normal morphology of secondary follicles of frozen‐cultured group was demonstrably lower than that of fresh‐cultured group(P<0 .05) .Conclusion Partial follicles might be injured in cryopreserved human ovarian tissues by direct cover vitrification .A period of post‐thaw culture may enhance follicle recovery .

Objective　To comprehend the psychological effects in the course of diagnosis and treatment of infertility on the sexuality, emotional feeling and marriage of women with tubal infertility. Methods　 The study involved 180 hospitalized patients who were awaiting tube operation from May to September in the year 2000.A survey was made by using questionnaire about sexuality, emotional feeling and marriage after infertility examinations. Results　 The sexual intercourse frequency of 46.2% infertile women decreased and no influences on sexual living satisfaction were confirmed during basal body temperature (BBT) examination. 92.3% to 93.2% of the infertile women were sad after hysterosalpingography(HSG) and laparoscopy. 78.9% of them felt remorse when the result of semen examination was normal. 56.6% of them would choose in vitro fertilization and embryo transfer(IVF-ET); 38.9% of the infertile couples' wedlock would not be stable and 10.5% of them would be divorced in the case of continuing infertility 2 years after tube operation. Conclusion　Sexual intercourse in almost half of the infertile couples decreased during BBT examination. Most infertile women became sad when they were informed of the abnormal results of HSG and laparoscopy and the normal results of semen analysis.

Objective　To identify the relationship between polycystic ovarian syndrome and hyperinsulinemia. Methods　Age, body height, body weight, serum estradiol, progesterone, testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin(PRL), triiodothyronine (T3), tetraiodothyronine (T4), insulin, oral glucose tolerance test (OGTT), and cortisol were measured in 28 patients with polycystic ovarian syndrome (PCOS).Results　Sxiteen patients (57.1% of total) had hyperinsulinemia. Among them one patient was found diabetic. No difference was noted in age, height, body weight, body mass index, serum estradiol, progesterone, testosterone, FSH, LH, PRL, T3, T4, cortisol, and glucose of OGTT between hyperinsulinemia and non-hyperinsulinemia patients. However the LH/FSH ratio(2.4±1.5) in hyperinsulinemia patients was significantly higher than 1.2±0.6 in non-hyperinsulinemia patients （P=0.0125）. In addition, the results showed that the fasting serum insulin, insulin after OGTT,the insulin/glucose ratio at 30, 120 minutes, 180 minutes, the area under curve of and the insulin, insulin resistant index (IRI) were higher in hyperinsulinemia pateints than in non-hyperinsulinemia patients respectively. Furthermore, the fasting insulin level, insulin/glucose ratio were found to be negatively related to insulin sensitivity index and positively related with β-cell functioning index. Conclusion　Hyperinsulinemia and insulin resistance accompany with some of the PCOS patients in China. Further investigations will be necessary to clarify the relevant mechanisms.