Objective To observe the clinical outcomes of double flap tympanic membrane repair with tragus and peri-chondrium under otoscope in the treatment of large tympanic membrane perforation.Methods Eighty patients with large tympanic membrane perforation admitted to Suzhou Kowloon Hospital,Shanghai Jiao Tong University School of Medicine from January 2018 to August 2022 were enrolled and divided into 2 groups by random number table method.Double flap tympanic membrane repair with tragus and peri-chondrium under microscope and otoscope were performed in microscope group and endoscopic group,respectively.The therapeutic effect was compared between the 2 groups.Results The operation time and hospital stay of the endoscopic group were shorter than those of the microscope group([49.02±7.77]min vs.[78.96±9.85]min,[4.89±1.02]d vs.[6.22±1.41]d),and intraoperative blood loss of the endoscopic group was less than that of the microscope group([7.21±2.25]ml vs.[14.55±5.23]ml).There was no significant difference in the incidence of complications between the 2 groups(P>0.05).Compared with preoperative ones,the auditory threshold of air conduction,auditory threshold of bone conduction,air bone gap and tinnitus grade were reduced in both groups 3 months after operation(P<0.05),while the scores of material life,social relationship,physical function and psychological function were increased,and the scores of the endoscopic group were significantly higher than those of the microscope group(P<0.05).There was no significant difference in the healing rate of eardrum 1 month or 3 months after operation between the 2 groups(P>0.05).Conclusion Otoscopic tympanic membrane repair has short operation time,less bleeding and quick postoperative recovery.It can improve hearing,alleviate tinnitus symptoms and improve patients'quality of life.

This paper reports the diagnosis and therapy of one case of Dipylidium caninum infection in an infant.

Objective To explore the relationship between the HLA-G 14 bp insertion/deletion polymorphism and the infection of Epstein-Barr virus(EBV) for children.Methods The study genotyped HLA-G 14 bp insertion/deletion polymorphism of 102 infectious mononucleosis children and 165 normal controls by PCR-PAGE,detected the plasma sHLA-G level of 51 infectious mononucleosis children and 146 normal controls by ELISA.Results A significant difference was observed for the frequencies of the HLA-G 14 bp genotype between the two groups( x2 =6.742,P=0.034 ),and a significant difference was also observed for the 14 bp allele frequencies between the two groups( x2 =6.672,P=0.01 ).The plasma sHLA-G levels in the infectious mononucleosis children were dramatically higher than that in normal controls,and a significant difference was observed between the two groups( Z=-9.472,P＜0.01 ).Among the infectious mononucleosis children,levels of sHLA-G was find a significant difference between the three genotypes of HLA-G 14 bp insertion/deletion polymorphism( H=6.09,P =0.048 ),and the level of s HLA-G with 14 bp+/+ genotype was markedly lower than that of the two other genotypes (Z=-2.376,P=0.01 8).Conclusion There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the infectious mononucleosis for children.Children who carried the 14 bp-/- genotype or deleted the 14 bp allele may have a significantly increased risk of the infection of EBV.The plasma sHLA-G might be considered as an index for auxiliary diagnosis infectious mononucleosis.