Purpose To explore the clinicopathological characteristics of atypical spindle cell lipomatous tumour(ASLT).Methods The clinical pathology,immunopheno-type,and molecular characteristics of 8 ASLTs diagnosed and treated in Fujian Cancer Hospital from 2020 to 2023 were col-lected.The histological morphology,immunophenotype and mo-lecular characteristics of 8 ASLTs were evaluated by HE stai-ning,immunohistochemistry and FISH.Clinicopathological fea-tures of 8 ASLTs were analyzed with literature review.Results Mixed distribution of spindle cells and adipocytes in different proportions was seen in all 8 cases,with no or mild cell atypia and no necrosis.Immunophenotypically,8 ASLTs were CD34 and S-100 positive to varying degrees,RB was negative,MDM2 and CDK4 were negative or focus positive,and Ki67 prolifera-tion index was low.FISH showed MDM2 amplification was nega-tive in 6 cases.Conclusion ASLT is a newly named benign adipose-derived tumor subtype with unique morphology,immu-nophenotype,and molecular characteristics.It has a good prog-nosis.Correct diagnosis can avoid clinical over-treatment.

In order to strengthen the business linkage and data sharing among member units of the municipal chest pain center, improve the efficiency of acute chest pain patient treatment, in 2023, Haikou People′s Hospital as the leading hospital of the chest pain center deploied a chest pain center information platform. The platform consisted of a data collection layer, a data center layer, a service layer, and an application layer through the network. It implemented unified data standards and data security measures, realized real-time interaction and sharing of the entire process of chest pain patient treatment data (comprehensive files of chest pain patients), and enhanced the collaborative treatment capabilities of various member units of the city chest pain center. As of March 2024, the information platform of Haikou Chest Pain Center had covered 32 medical institutions and shared treatment data of 12 105 patients. From April 2023 to March 2024, the average time from the completion of the first electrocardiogram report of chest pain center patients to their diagnosis was about 3.8 minutes, which was significantly reduced about 75% compared to before the platform application (April 2022 to March 2023), so the treatment efficiency of chest pain patients was effectively improved. The construction practice of this chest pain center information platform could provide references for the informationization construction of chest pain centers in other regions of China.

In order to strengthen the business linkage and data sharing among member units of the municipal chest pain center, improve the efficiency of acute chest pain patient treatment, in 2023, Haikou People′s Hospital as the leading hospital of the chest pain center deploied a chest pain center information platform. The platform consisted of a data collection layer, a data center layer, a service layer, and an application layer through the network. It implemented unified data standards and data security measures, realized real-time interaction and sharing of the entire process of chest pain patient treatment data (comprehensive files of chest pain patients), and enhanced the collaborative treatment capabilities of various member units of the city chest pain center. As of March 2024, the information platform of Haikou Chest Pain Center had covered 32 medical institutions and shared treatment data of 12 105 patients. From April 2023 to March 2024, the average time from the completion of the first electrocardiogram report of chest pain center patients to their diagnosis was about 3.8 minutes, which was significantly reduced about 75% compared to before the platform application (April 2022 to March 2023), so the treatment efficiency of chest pain patients was effectively improved. The construction practice of this chest pain center information platform could provide references for the informationization construction of chest pain centers in other regions of China.

Objective:To construct a eukaryotic expression plasmid pcDNA3.1(+)-BmCPI502/BmGAPDH720 of periodic Brugia malayi cysteine protease inhibitor/glyceraldehyde 3-phosphate dehydrogenase (CPI502/GAPDH720) multi-epitope gene and observe its protein expression in Hela cells. Methods:Primers were designed according to the predicted gene sequences of T cell epitopes. The target gene fragment was amplified by reverse transcription (RT)-PCR using plasmid pGEM-T-CPI621 as template. The fragment was cloned into prokaryotic expression plasmid pET-28a(+) to construct prokaryotic expression plasmid pET28a(+)-BmCPI502. The BmCPI502 gene and BmGAPDH720 gene were amplified by RT-PCR, respectively. The gene fragments of pcDNA3.1(+), BmCPI502 and BmGAPDH720 were digested by double enzyme digestion and ligated with the target gene. The eukaryotic expression plasmid pcDNA3.1(+)-BmCPI502/BmGAPDH720 was constructed. The recombinant plasmid was transfected into Hela cells and verified by RT-PCR to obtain the desired target bands. The expression product was detected by sodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE).Results:The recombinant plasmid pET28a(+)-BmCPI502 was obtained, and the 502 bp specific fragment was identified by enzyme digestion, which was in line with the expected value; the eukaryotic expression plasmid pcDNA3.1(+)-BmCPI502/BmGAPDH720 of the multi-epitope gene was successfully constructed, and the fragment size was in line with the expected value. The eukaryotic expression plasmid pcDNA3.1(+)-BmCPI502/BmGAPDH720 was transfected into Hela cells and the recombinant protein was stable expressed. SDS-PAGE analysis showed that the relative molecular weight ( Mr × 10 3) of the recombinant protein was about 50. Conclusions:The eukaryotic expression plasmid of pcDNA3.1(+)-BmCPI502/BmGAPDH720 of periodic Brugia malayi multi-epitope gene is successfully constructed, and the corresponding recombinant protein is obtained in eukaryotic cells. This study has laid a foundation for further study of the purification and biological activity of the recombinant protein.

Peripartum cardiomyopathy (PPCM) often develops in the early postpartum period, especially within one month after delivery. However, in recent years, some cases of pregnancy-related cardiomyopathy with earlier or slightly later onset showed similar features with PPCM entirely. One patient with PPCM was admitted to the Department of Intensive Care Medicine of the First Affiliated Hospital of Bengbu Medical College on February 2022. After admission, the patient was exceptionally hemodynamically unstable and had repeated ventricular fibrillation. Apart from extracorporeal membrane oxygenation (ECMO) support, comprehensive treatment with cardiotonic agents and drugs to inhibit the "ventricular electric storm" was given. The cardiac structure and function were gradually improved. ECMO was successfully withdrawn on the 7th day after the onset of symptoms, and the patient was discharged after a full recovery. Early diagnosis and active intervention are the keys to improve the prognosis of patients with PPCM and avoid irreversible changes in cardiac structure.

To establish an intelligent cervical vertebra maturity assessment system, and to evaluate the reliability and clinical value of the system. Sixty children aged were recruited in the study. Lateral cephalometric radiograph and cone beam CT (CBCT) were taken at the same period. Based on the CBCT data, the system automatically extracted the patient's facial area through Otsu's method, intercepted the sagittal plane by three-dimensional least squares method, captured the second to fourth cervical vertebrae by superpixel segmentation. And then selected points were marked automatically through morphological algorithm and manual method. Consistency test was performed on the two sets of data to compare the reliability of automated cervical morphology capture. According to the parameters of morphological identification, positioning and staging algorithms were designed to form the intelligent cervical vertebra maturity assessment system. The cervical vertebra maturity was also judged manually on the lateral cephalometric radiograph. The weighted Kappa test and the Gamma correlation coefficient were subsequently applied to evaluate the consistency and correlation. The results showed that the cervical vertebra features automatically captured based on CBCT data had a high accuracy on the overall morphological recognition. In the prediction of 8 inflection points out of 13 points, there was no significant difference between automatic and manual method on both X and Y axes (all >0.05). The assessment results of the cervical vertebra maturity of the intelligent system had strong consistency and correlation with the manual recognition results (weighted Kappa value=0.877, Gamma value=0.991, both <0.05). The intelligent cervical vertebrae maturity assessment system based on CBCT data established in this study presents reliable outcome and high degree of automation, indicating that the system may be used clinically.
Cephalometry ; Cervical Vertebrae/diagnostic imaging* ; Child ; Cone-Beam Computed Tomography ; Humans ; Radiography ; Reproducibility of Results

Animals ; Centromere ; metabolism ; Chromosomal Proteins, Non-Histone ; metabolism ; Mice ; Models, Molecular ; Synaptonemal Complex ; metabolism

ABSTRACT:Objective To investigate the effects of the holistic health education system in patients after coronary stent.Methods A total of 720 patients after coronary stent were randomly divided into observation group with 360 cases and control group with 360 cases,the control group was conducted health education at admission and discharge,observation group were carried out whole-course tracking health education based on the control group.Drug therapy compliance, master of secondary prevention knowledge,mastering related diseases,nursing satisfaction,read-mission rates and mortality were compared between the two groups.Results Drug therapy com-pliance,master of secondary prevention knowledge,mastering related diseases,nursing satisfaction in the observation group were better than that in the control group(P <0.05);Readmission rates and mortality were lower than that in the control group (P <0.05).Conclusion Implementation of holistic health education system management in the patients after coronary stent can reduce pa-tient′s risk factors,improve the quality of life,increase the rate of postoperative rehabilitation, strengthen effective drug therapy compliance,correct patient′s bad living habits,reduce readmis-sion rates,and improve nursing satisfaction,so it is worthy of clinical promotion.

ABSTRACT:Objective To investigate the effects of the holistic health education system in patients after coronary stent.Methods A total of 720 patients after coronary stent were randomly divided into observation group with 360 cases and control group with 360 cases,the control group was conducted health education at admission and discharge,observation group were carried out whole-course tracking health education based on the control group.Drug therapy compliance, master of secondary prevention knowledge,mastering related diseases,nursing satisfaction,read-mission rates and mortality were compared between the two groups.Results Drug therapy com-pliance,master of secondary prevention knowledge,mastering related diseases,nursing satisfaction in the observation group were better than that in the control group(P <0.05);Readmission rates and mortality were lower than that in the control group (P <0.05).Conclusion Implementation of holistic health education system management in the patients after coronary stent can reduce pa-tient′s risk factors,improve the quality of life,increase the rate of postoperative rehabilitation, strengthen effective drug therapy compliance,correct patient′s bad living habits,reduce readmis-sion rates,and improve nursing satisfaction,so it is worthy of clinical promotion.

Objective To investigate the oropharyngeal microbial colonization in elderly patients.Methods Totally 618 elderly cases and 96 young and middle-aged cases were involved in this investigation.The colonization of bacteria and fungi on the oropharyngeal mucosa were obtained by throat swab cultures.Results There were 85.4％ of cases (82 cases) with Streptococcus viridans and Neisseria gonorrheae on the oropharynx mucosa in young and middle-aged group and the pattern of constitute was simple.3 to 5 bacterial species were isolated from the oropharyngeal mucosa in elderly group.There were only 25.7％ of cases (159 cases)with Streptococcus viridans and Neisseria gonorrheae on the oropharyngeal mucosa in elderly group.Biodiversity of the constitution pattern in the bacterial colonization was exhibited in the elderly.The colonization rate of Gramnegative bacilli was higher in elderly group than in young and middle-aged group [53.1％ (328/618)vs.6.3％ (6/96)].Staphylococcus aureus and Streptococcus pneumonia were the common Grampositive bacteria colonization in the elderly.The colonization rate of Candida fungus was 9.1 ％ (56/618) in the elderly.Conclusions The reduction of commensal bacteria,especially Streptococcus viridans may be the pathological basis of mode changes in bacteria colonization and opportunistic bacteria colonization on oropharyngeal mucosa in the elderly.The colonization rate of oropharyngeal Gram-negative bacili is obviously increased and Klebsiella Pneumoniae is the common bacteria on oropharyngeal mucosa in the elderly.Enterobacteriaceae,Pseudomonas aeruginosa and Acinetobacter baumannii are sensitive to common antibiotics.