Objective:To explore the application effect of group guidance based on intelligent communication matrix in patients with type 2 diabetes mellitus (T2DM) .Methods:Using the convenient sampling method, a total of 60 T2DM patients with poor self-management ability who scored less than 19.6 on Summary of Diabetes Self Care Activities (SDSCA) in the Affiliated Chuzhou Hospital of Anhui Medical University were selected as the research objects from May to June 2020. They were randomly divide into the observation group ( n=30) and the control group ( n=30). The observation group was given continuous group guidance based on intelligent communication matrix, while the control group was given routine health guidance. The SDSCA score, body mass index and glycosylated hemoglobin (HbA1c) before and after intervention were compared between the two groups. Results:After intervention, the SDSCA score of the observation group was higher than that of the control group, HbA1c was lower than that of the control group and body mass index was lower than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The group guidance based on intelligent communication matrix can improve the self-management level of T2DM patients and reduce their body mass index and HbA1c levels, which has clinical application value.

Objective To construct a rapid prediction system to improve the accuracy and efficiency of evaluation of the consequences of nuclear accidents at a field scale. Methods Base on a diagnostic wind field model and Lagrangian particle diffusion, we established a rapid prediction method for wind field and pollutant dispersion around complex underlying surfaces within a field scale, in a way of visual discrimination of buildings and vegetation distribution. With data simulation and the use of a real urban field example, the simulated results were compared with wind tunnel test measurements and computational fluid dynamics results to study the influence of complex underlying surfaces on wind field and pollutant transport in the region. Results The rapid prediction system could clearly simulate the high-resolution wind field and pollutant concentration distribution of the region in about five minutes. It could interface with geographic information software and couple with a mesoscale weather prediction model. In terms of accuracy, the system performed well in wind field simulation, with the fractional deviations of wind speed and wind direction being 0.33 and −0.08, respectively. Concentration field simulation was greatly affected by the wind field, and the ratios of simulated concentrations to observed concentrations were between 0.05 and 3.4, except for a few low concentration points. Conclusion The rapid prediction system can effectively simulate the distribution characteristics of the flow field and improve calculation efficiency when ensuring calculation accuracy, which provides an important reference for emergency response to nuclear accidents.

Objective To investigate the clinical,laboratory and MRI imaging features of a spontaneous spinal subarachnoid hemorrhage(S-SAH)so as to improve the recognition of spontaneous (S-SAH).Methods The data of clinical,laboratory,MRI imaging features,treatment and prognosis of the spontaneous(S-SAH)were collected and statistically analyzed.Relevant literatures were then reviewed.Results The patient was a 75-year-old woman with neuromyelitisoptica spectrum disorders.During the course of hospitalization for intravenous infusion of high-dose methylprednisolone,the patient suffered from a sudden and severe migrating back pain that gradually progressed from the back to the neck and head,and signs of meningeal irritation occurred.After the exclusion of aortic dissection,acute coronary syndrome,pulmonary embolism,and fractures,further MRI scans of the head and spinal cord were performed.The MRI confirmed subarachnoid hemorrhage located in spinal cord and posterior cranial foss,and the diagnosis was spontaneous S-SAH.Conclusions Migrating back pain combined with signs of meningeal irritation is a characteristic clinical manifestation of S-SAH,and a prompt MRI evaluation of spinal cord is helpful to improve the early diagnosis and treatment.

Objective Recent evidence has implicated Larp1,an RNA-binding protein,in cell motility and EMT therefore prompting the study of Larp1 in EOC.This project aims to examine the potential role of Larp1 in cell invasion.Methods Ovarian cancer cells SKOV-3 and OVCAR-3 were transfected with DNA constructs to overexpress Larp1 and transfect cells were used to assess the overexpression of Larp1 in cell invasion and metastasis using several functional assay.Results Larp1 overexpression in both OVCAR-3 and SKOV-3 cells appear to enhance the invasive ability of cells and the accumulation of Larp 1 in these chemoinvasive protrusions strongly suggests the potential role of Larp1 in invadopodia formation.Cell spot chemoinvasion assay demonstrated increased chemoinvasion of Larp1 overexpressing SKOV-3 and OVCAR-3 cells towards a gradient of TGF-β,EGF and bFGF.Conclusions With its potential role in EMT and cell invasion,a deeper understanding of the physiological role of Larp1 in cancer cell motility will be potentially beneficial in the diagnosis and treatments of ovarian cancer.

Objective:To investigate the effects of laparoscopy assisted complete mesocolon resection on the stress response and immune function of patients with colon cancer.Methods:From January 2013 to January 2016,130 patients with colon cancer in our hospital were selected and randomly divided into the observation group and the control group with 65 cases in each group according to the random lottery envelopes.The observation group was treated with laparoscopy assisted complete mesocolon resection,the control group was treated with open operation.The changes of semm IL-6,TNF-α and amours of CD4+ cells,blood loss,bowel function restoration time,hospital stays and complication rate of two groups were compared before and after treatment.Results:Both groups of patients were successfully completed the surgery and there was no serious complications occurred in both groups.The amount of bleeding,postoperative intestinal recovery time and postoperative hospital stay in the observation group were significantly less than those of the control group (P＜0.05).The chylous fistula,wound infection,pulmonary infection and complication rate on the 14th day postoperation in the observation group was 1.5％,which was 12.3％ in the control group and significantly higher than that of the control group (P＜0.05).The contents ofCD4+ cells in the observation group and the control group were 36.34± 7.83％ and 33.66± 9.82％,respectively,which were significantly higher than those before treatment(30.33± 6.49％ and 30.49± 5.33％)(P＜0.05),and the contents of CD4+ cells of observation group was significantly higher than that of the control group after treatment (P＜0.05).The serum levels ofIL-6 and TNF-α in both groups were significantly lower than those before treatment on the 14th day postoperation (P＜0.05),and the serum IL-6 and TNF-α levels of observation group were significantly lower than those in the control group on the 14th day postoperation (P＜0.05).Conclusion:Laparoscopy assisted complete mesocolon resection could promote the immune function and inhibit the stress response,which further promoted the rehabilitation and induced the complications in the treatment of patients with colon cancer.

Objective To investigate the role of the activated rhuPAa-melittin in ovarian cancer cells and to study the inhibitory effect of rhuPAa-melittinon on ovarian cancer cells. Methods rhuPAa-melittin was used to treat the ovarian cancer cells at different concentrations for 48 hrs. Then flow cytometery was applied to detect the cell cycle and cell apoptosis. rhuPAa-melittin protein was delivered to the mouse mode to investigate the effect of rhuPAa-melittin on the growth of the xenotransplanted tumor. Results rhuPAa-melittin was used to treat ovarian cancer cells at the concentration of 0 ,4 ,8 and 16 μg/mL for 48 hrs ,respectively. The results of cell apoptosis assay was 1.16%,3.83%,6.51% and 10.2%,respectively. Moreover,different concentrations of rhuPAa-melittin had no effects on the cells at G0/G1 phase,rhuPAa-melittin inhibited S phase cells to process into G2/M phase, contributing to suppressing the growth of ovarian cancer cells. Conclusion The in vivo and in vitro studies revealed that rhuPAa-melittin inhibits the growth of ovarian cancer.

OBJECTIVE:To work out the optimal inventory levels in zero inventory management mode through model predic-tion and control strategy,by using the inventory upper & lower limits settings generally available in the information management system of health care institutions. METHODS:Multi-varieties joint ordering modelwas constructed by referring to operations management,time series analysis and quantitative approach to decision-making,that is,to make a prediction of upper&lower lim-its on medicine inventory based on historical data and applicable mathematical models(fixed order interval model and re-order mod-el,i.e. FOI and ROP),and compared with real results;based on above,specific medicine procurement and inventory control strat-egies would be developed and an evaluation of the application effects would be made. RESULTS:The error test and reproducibility test exhibited that the out-of-stock ratio remained under 3.36%,of which 71.24% could be effectively alarmed;under computer simulation and practical operation,the instant replenishment rate reduced by 9.33% and 13.03%,OOS ratio down by 11.11% and 27.45%,and average daily inventory turnover rate up by 30.19% and 15.85% respectively,all showing remarkable improvements compared to before the implementation of the mode. CONCLUSIONS:This model is of favorable accuracy and operability,there-fore it can lay foundation for rational and well-founded decisions in medicine procurement and inventory control in zero inventory management mode.

Objective RhuPAa-melittin protein with high-purity was analyzed to investigate its biologic effect on normal and ovarian cancer cells.Methods Preliminary purified rhuPAa-melittin was added in medium normal cells and SKOV3 human ovarian cancer cells cultured for 48 hours,and then were measured at 490 nm wavelength absorbance to measure the number of living cells and evalu-ate the inhibitory effect of rhuPAa-melittin on cultured cells.Results The inhibitory rate of rhuPAa-melittin at 4 μg/mL was 66.59% in SKOV3 cancer cell progression.At a concentration of 16 μg/mL, rhuPAa-melittin killed all cancer cells (P <0.05).In normal adult fibrous cells,the inhibitory rate of rhuPAa-melittin at 4 μg /mL was only 7.3%,and 12.3% inhibition rate was obtained by using rhu-PAa-melittin (8 μg/mL).Moreover,inhibition rate topped at 22.0% when used rhuPAa-melittin at 16 μg/mL.Conclusion RhuPAa-melittin has a strong anti-tumor effect on SKOV3 cancer cells.

Objective RhuPAa-melittin protein with high-purity was analyzed to investigate its biologic effect on normal and ovarian cancer cells.Methods Preliminary purified rhuPAa-melittin was added in medium normal cells and SKOV3 human ovarian cancer cells cultured for 48 hours,and then were measured at 490 nm wavelength absorbance to measure the number of living cells and evalu-ate the inhibitory effect of rhuPAa-melittin on cultured cells.Results The inhibitory rate of rhuPAa-melittin at 4 μg/mL was 66.59% in SKOV3 cancer cell progression.At a concentration of 16 μg/mL, rhuPAa-melittin killed all cancer cells (P <0.05).In normal adult fibrous cells,the inhibitory rate of rhuPAa-melittin at 4 μg /mL was only 7.3%,and 12.3% inhibition rate was obtained by using rhu-PAa-melittin (8 μg/mL).Moreover,inhibition rate topped at 22.0% when used rhuPAa-melittin at 16 μg/mL.Conclusion RhuPAa-melittin has a strong anti-tumor effect on SKOV3 cancer cells.