Idiopathic pulmonary fibrosis (IPF), a chronic interstitial lung disease, is characterized by aberrant wound healing, excessive scarring and the formation of myofibroblastic foci. Although the role of alternative splicing (AS) in the pathogenesis of organ fibrosis has garnered increasing attention, its specific contribution to pulmonary fibrosis remains incompletely understood. In this study, we identified an up-regulation of serine/arginine-rich splicing factor 7 (SRSF7) in lung fibroblasts derived from IPF patients and a bleomycin (BLM)-induced mouse model, and further characterized its functional role in both human fetal lung fibroblasts and mice. We demonstrated that enhanced expression of Srsf7 in mice spontaneously induced alveolar collagen accumulation. Mechanistically, we investigated alternative splicing events and revealed that SRSF7 modulates the alternative splicing of pyruvate kinase (PKM), leading to metabolic dysregulation and fibroblast activation. In vivo studies showed that fibroblast-specific knockout of Srsf7 in conditional knockout mice conferred resistance to bleomycin-induced pulmonary fibrosis. Importantly, through drug screening, we identified lomitapide as a novel modulator of SRSF7, which effectively mitigated experimental pulmonary fibrosis. Collectively, our findings elucidate a molecular pathway by which SRSF7 drives fibroblast metabolic dysregulation and propose a potential therapeutic strategy for pulmonary fibrosis.

Objective To explore the application of high quality nursing intervention in elderly patients with acute exacerbation of COPD complicated with respiratory failure and its influence on serum inflammatory factors.Methods A total of 100 elderly COPD patients with respiratory failure in ICU were randomly divided into observation group (50 cases) with high quality nursing intervention,and control group (50 cases) with routine nursing according to random table method,pulmonary function improvement,and level changes of serum inflammatory factors after 2 week follow-up of two groups were observed,and quality of nursing intervention was compared.Results There were significant differences in lung function index (FEV1,FEV1/FVC and FEV1％) after treatment compared with treatment before,and observation group was significantly better than the control group (P ＜0.05).There was a significant difference between the two groups after treatment in levels of serum inflammatory factors (IL-1,IL-2,TNF-α,FIN-γ) than treatment before,and the observation group was significantly better than the control group (P ＜ 0.05).The observation group had better conditions in hospitalization time,hospitalization cost and nursing satisfaction than the control group (P ＜0.05).Conclusion Quality care can obviously improve clinical symptoms,lung function and level of serum inflammatory factors,and can improve compliance and clinical nursing satisfaction,so it is worthy of promotion.

Objective To explore the application of high quality nursing intervention in elderly patients with acute exacerbation of COPD complicated with respiratory failure and its influence on serum inflammatory factors.Methods A total of 100 elderly COPD patients with respiratory failure in ICU were randomly divided into observation group (50 cases) with high quality nursing intervention,and control group (50 cases) with routine nursing according to random table method,pulmonary function improvement,and level changes of serum inflammatory factors after 2 week follow-up of two groups were observed,and quality of nursing intervention was compared.Results There were significant differences in lung function index (FEV1,FEV1/FVC and FEV1％) after treatment compared with treatment before,and observation group was significantly better than the control group (P ＜0.05).There was a significant difference between the two groups after treatment in levels of serum inflammatory factors (IL-1,IL-2,TNF-α,FIN-γ) than treatment before,and the observation group was significantly better than the control group (P ＜ 0.05).The observation group had better conditions in hospitalization time,hospitalization cost and nursing satisfaction than the control group (P ＜0.05).Conclusion Quality care can obviously improve clinical symptoms,lung function and level of serum inflammatory factors,and can improve compliance and clinical nursing satisfaction,so it is worthy of promotion.

This paper reviewed the studies on the effect of electroacupuncture in improving learning and memory functions in the past 10 years. It showed an overview of the mechanisms that how acupuncture worksed: promoting synaptic plasticity, regulation of microvascular injury and oxidative stress; slowing down the progress of brain cell apoptosis, inhibition of inflammatory response;regulating neurotransmitter release and protecting the ultrastructure of neurons. In addition, this paper puts forth the problems which left in the current studies.

Objective To study the effects of previous analgesia of acupuncture on patients with the mixed hemorrhoid surgery pain.MethodsA total of 70 patients with mixed hemorrhoid treated with “Milligan-morgan hemorrhoids” were randomly divided into treatment group and control group, 35 patients in each group. The treatment group was treated 30 min prior to the surgery with needling and manipulating Baliao, Chengshan, Hegu every five minutes until the operation, while the control group was not treated before the operation. The patients were assessed by Visual Aualogue Scale and self-reporting Inventory.ResultsAfter the operation, the treatment group was significantly better than the control group in the outcome index of beginning time of pain (14.3 ± 4.9 hvs. 4.2 ± 2.3 h, Z=-5.666,P<0.01) and peak time of pain (17.3 ± 4.5 hvs. 6.0 ± 2.9 h,Z=-5.581,P<0.01). The treatment group was significantly better than the control group in decreasing the pain beginning VAS score (3.3 ± 1.7vs. 4.6 ± 1.7,Z=-2.820, P<0.01) and pain peak VAS score (4.5 ± 2.0vs. 6.5 ± 1.2,Z=-4.025,P<0.05). After surgery, the treatment group was significantly better than the control group in decreasing the score of Self-reporting Inventory scale at the 1stday (1.8 ± 1.3vs. 3.0 ± 1.3),Z=-3.472,P<0.01) and 2ndday (1.2 ± 0.9vs. 1.9 ± 1.2,Z=-2.464,P<0.05). And the treatment group was significantly better than the control group inreducing the quantities of compound aminopyrine phenacetin tablet (0.5 ± 0.9vs.1.5 ± 1.7,t=3.167,P=0.002).ConclusionAcupuncture analgesia 30 minutes prior to the mixed hemorrhoid surgery can significantly reduce the postoperative pain.

Objective To observe the clinical efficacy of surround electroacupuncture in relieving pain after operation for mixed hemorrhoids. Method Seventy-two patients with mixed hemorrhoids were randomized into a treatment group and a control group, 36 cases in each group. The two groups both received the corresponding interventions once in 15 min after the operation: the treatment group received electroacupuncture treatment, and the control group was only placed in a simulated environment of electroacupuncture but without receivimg any needling treatment. The pain intensity was evaluated by using Verbal Rating Scale (VRS), Wong-Baker Faces Pain Rating Scale, and Visual Analogue Scale (VAS) at 11 different time points after the treatment: once every 1 h in 8 h after the treatment, once at the first defecation, once respectively 24 h and 48 h after the treatment. The data were statistically analyzed afterwards. Result At 3 h and 4 h after the treatment and at the first defecation after the treatment, the VRS scores of the treatment group were significantly lower than that of the control group (P<0.05); respectively at 4 h, 5 h, 6 h, 7 h, and 8 h after the treatment and at the first defecation, the Wong-Baker scores of the treatment group were significantly lower than that of the control group (P<0.05); respectively at 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, and 8 h after the treatment, at the first defecation, as well as at 24 h after the treatment, the VAS scores of the treatment group were significantly lower than that of the control group (P<0.05); it indicates that the pain intensity of the treatment group should be lower than that of the control group at the above time points, and the analgesic effect in the treatment group lasts a longer time, running a lower risk of losing effectiveness compared to the control group (P<0.05). Conclusion Real-time acupuncture treatment after the operation is significantly effective in relieving pain after operation for hemorrhoids.

Objective To explore the effects of electroacupuncture (EA) on behaviors and hippocampal extracellular regulated protein kinases (ERK)/cAMP-response element binding protein (CREB) expressions in depression model rats.Methods Forty male SD rats were divided into 4 groups randomly:normal group,model group,EA group and sham EA group (n=10).Chronic unpredictable mild stress (CUMS) and isolation were performed to establish the depression models.EA was applied to Yintang (EX-HN3) and Baihui (GV20) 10 min once a day for two weeks in EA group;sham EA group received placebo treatment.After 4 weeks of intervention,the sucrose intake test,forced swim test and Morris water maze test were used to measure the antidepressant-like effect of EA treatment.The expressions of ERK and CREB in the hippocampus were detected by Western blotting.Results As compared with the model group,the EA group had significantly improved sucrose solution intake ([62.080±11.995] ％ vs.[74.881±3.308] ％) and statistically decreased immobility time ([123.668± 18.925] s vs.[93.012±15.957] s) in forced swimming (P＜0.05).In Morris water maze test,the escape latency to find the target platform became gradually shorter and the frequency of crossing platform significantly increased in EA group (0.900±1.875 vs.3.200±1.549,P＜0.05).The phosphorylated(p)-ERK and p-CREB levels in the EA group were significantly increased as compared with those in the model group (P＜0.05).Conclusion EA could improve depression-like behaviors by increasing the levels of p-ERK and p-CREB in the hippocampus.

Purpose To investigate the clinicopathological features, treatment and prognosis of perivascular epithelioid cell tumor ( PEComa) of the head and neck. Methods Two cases of PEComa were analyzed by studying clinical data, histopathologic and immu-nohistochemical ( EnVision) changes and the related literatures were reviewed. Results Case 1 was a 26-year-old woman with a re-current larynx tumor, and case 2 was diagnosed in a 56-year-old man with a left nasal cavity mass. Their tumors were well-demarcated, and ranged from 2. 5 to 3. 5 cm in diameter. Microscopically, the tumors were composed of epithelioid cells arranged in nets, organoid pattern. The tumor cells had abundant pale eosinophilic granular to clear cytoplasm, vesicular nuclei, and mild atypia. The stroma was rich in capillaries, sinusoidal vasculature. There was mitotic activity and focal necrosis. By immunohistochemistry, the tumor cells were diffusely or focally positive for vimentin (2/2), HMB-45(1/2), Melan-A(2/2), SMA(2/2), Calponin(2/2), desmin(1/2), and TFE3(1/2). They were negative for CK, CD10, S-100, CgA, Syn, and MyoD1. Conclusion PEComa of the head and neck is rare. Definite diagnosis depends upon the comprehensive analysis of histopathology and coexpression of melanocytic and myoid mark-ers. The differential diagnosis include paraganglioma, malignant melanoma and metastatic carcinoma. Few PEComas of the head and neck behaved in an aggressive fashion with distant or loco-regional metastases and died of disease-related causes, it should be regarded as tumors with a malignant potential.

The pharmacokinetics and relative bioavailability were studied in 18 healthy volunteers. A single oral dose of 150 mg irbesartan capsule (test) or tablet (reference) was given to each volunteer according to a randomized 2-way crossover study. The concentrations in plasma were determined by HPLC-UV method. The main parameters of irbesartan capsules were: Cmax: 1.502 +/- 0.295 micrograms/ml, tmax: 1.44 +/- 0.34 h, t 1/2: 20.21 +/- 14.71 h, AUC0-t: 11.087 +/- 3.443 micrograms/ml-1.h. The relative bioavailability of capsule to tablet was (101.4 +/- 28.9)%. The results of statistical analysis showed that two formulations were bioequivalent.
Adult ; Angiotensin Receptor Antagonists ; Biological Availability ; Biphenyl Compounds ; blood ; pharmacokinetics ; Capsules ; Cross-Over Studies ; Humans ; Male ; Tablets ; Tetrazoles ; blood ; pharmacokinetics

The pharmacokinetics and relative bioavailability were studied in 18 healthy volunteers. A single oral dose of 150 mg irbesartan capsule (test) or tablet (reference) was given to each volunteer according to a randomized 2-way crossover study. The concentrations in plasma were determined by HPLC-UV method. The main parameters of irbesartan capsules were: Cmax: 1.502 +/- 0.295 micrograms/ml, tmax: 1.44 +/- 0.34 h, t 1/2: 20.21 +/- 14.71 h, AUC0-t: 11.087 +/- 3.443 micrograms/ml-1.h. The relative bioavailability of capsule to tablet was (101.4 +/- 28.9)%. The results of statistical analysis showed that two formulations were bioequivalent.
Biological Availability ; Biphenyl Compounds/blood ; Biphenyl Compounds/*pharmacokinetics ; Capsules ; Cross-Over Studies ; Receptors, Angiotensin/*antagonists & inhibitors ; Tablets ; Tetrazoles/blood ; Tetrazoles/*pharmacokinetics