BACKGROUND:Developing a material that promotes alveolar bone regeneration and has good properties is of great significance for the treatment of alveolar bone defects.OBJECTIVE:To summarize the research on alveolar bone defect regeneration and repair in the past 5 years,classify and introduce new materials and drugs,so as to grasp the latest progress in related fields.METHODS:"Alveolar bone,alveolar bone defect,alveolar bone regeneration,mechanism,biomaterials,nanoscaffolds,hydrogels,medications,anti-inflammatory drugs,simvastatin,metformin,traditional Chinese medicine,growth factor,stem cell"were used as search terms in Chinese and English for literature retrieval in CNKI and PubMed,respectively.A total of 117 articles were finally obtained for review and analysis.RESULTS AND CONCLUSION:The application of biomaterials provides a scaffold structure for alveolar bone regeneration and can also be used as a bone substitute to repair bone defects.Topical and systemic applications of Western and Chinese medicines can control inflammation and promote bone regeneration.Materials loaded with various growth factors have the effect of osteogenesis and can promote the repair of alveolar bone defects.In stem cell tissue engineering,the seed cells are osteogenic and fibrogenic,which can differentiate into osteoblasts to generate new bone.In recent years,research has been keen on the combination of the above to develop new,biocompatible,and slow-release drugs or materials to promote the regeneration and repair of alveolar bone defects.

BACKGROUND:Developing a material that promotes alveolar bone regeneration and has good properties is of great significance for the treatment of alveolar bone defects.OBJECTIVE:To summarize the research on alveolar bone defect regeneration and repair in the past 5 years,classify and introduce new materials and drugs,so as to grasp the latest progress in related fields.METHODS:"Alveolar bone,alveolar bone defect,alveolar bone regeneration,mechanism,biomaterials,nanoscaffolds,hydrogels,medications,anti-inflammatory drugs,simvastatin,metformin,traditional Chinese medicine,growth factor,stem cell"were used as search terms in Chinese and English for literature retrieval in CNKI and PubMed,respectively.A total of 117 articles were finally obtained for review and analysis.RESULTS AND CONCLUSION:The application of biomaterials provides a scaffold structure for alveolar bone regeneration and can also be used as a bone substitute to repair bone defects.Topical and systemic applications of Western and Chinese medicines can control inflammation and promote bone regeneration.Materials loaded with various growth factors have the effect of osteogenesis and can promote the repair of alveolar bone defects.In stem cell tissue engineering,the seed cells are osteogenic and fibrogenic,which can differentiate into osteoblasts to generate new bone.In recent years,research has been keen on the combination of the above to develop new,biocompatible,and slow-release drugs or materials to promote the regeneration and repair of alveolar bone defects.

Objective:To investigate the influencing factors of adult dental fluorosis in drinking water-borne endemic fluorosis areas of Inner Mongolia Autonomous Region.Methods:A case-control study was conducted in January 2024 to select adult fluorosis patients (case group) and healthy individuals (control group) from the drinking water-borne endemic fluorosis areas in Helinger County, Hohhot City, Inner Mongolia Autonomous Region as the survey subjects. Urine samples were collected to determine urinary fluoride concentration. A questionnaire survey was conducted. SPSS 25.0 software was used for χ 2 test and multivariate logistic regression analysis. Restricted cubic spline (RCS) was used to analyze the association between urinary fluoride concentration and the risk of dental fluorosis in adults. Results:A total of 161 individuals were included in the survey, including 100 in the case group and 61 in the control group. The results of univariate analysis showed that there were statistically significant differences in the distribution of gender, smoking, and urinary fluoride concentration between the case group and the control group (χ 2 = 7.54, 5.02, 9.69, P < 0.05). The results of multivariate logistic regression analysis indicated that gender ( OR = 0.36, 95% CI: 0.18 - 0.73, P = 0.005) and urinary fluoride concentration ( OR = 3.08, 95% CI: 1.46 - 6.67, P = 0.003) were the influencing factors of adult fluorosis. RCS analysis showed a significant linear dose-response relationship between the risk of dental fluorosis and urinary fluoride concentration ( Poverall trend = 0.001, Pnonlinear = 0.071). When the urinary fluoride concentration was greater than 1.57 mg/L, the risk of dental fluorosis increased with the increase of urinary fluoride concentration. Conclusion:Gender and urinary fluoride concentration are the risk factors of dental fluorosis in adults in drinking water-borne endemic fluorosis areas of Inner Mongolia Autonomous Region.

Objective To explore the circRNA expression level in the urine of patients with lupus nephritis(LN),to construct a diagnostic model for LN,and to evaluate and verify its diagnostic efficacy.Methods A total of 120 patients with LN admitted from January 2022 to January 2024 were selected as the observation group and divided into the training group(n=84)and the validation group(n=36)at a ratio of 7:3.Another 100 healthy individuals who underwent physical examination were selected as the control group.The expression levels of circRNA-0001445,circRNA-002059,hsa-circ-0007385 and hsa-circ-0000006 in urine specimens of patients in the training group and the control group were detected by gene sequencing.Multivariate Logistic regression analysis was used to construct a diagnostic model for LN based on the urinary circRNA expression level.The diagnostic value of individual indicators and combined predictive models were evaluated by receiver operating characteristic(ROC)curve analysis,and then the predictive efficacy of the model was verified by the validation group.Results The expression levels of circRNA-0001445,circRNA-002059,hsa-circ-0007385 and hsa-circ-0000006 in the urine of patients in the training group were all higher than those of the control group(P<0.01).Multivariate Logistic regression analysis found that The expression levels of circRNA-0001445(OR=1.094),circRNA-002059(OR=1.040),hsa-circ-0007385(OR=1.033),and hsa-circ-0000006(OR=1.130)in theurine were independent factor for the diagnosis of LN(P<0.01).ROC curve analysis showed that the areas under the curve of combined model constructed based on the circRNA expression level in the urine were 0.985 and 0.911 in the training group and the validation group,respectively,with sensitivities of 95.00%and 91.67%,and specificities of 99.00%and 89.00%,all of which were higher than the diagnostic efficacy of a single urinary circRNA expression level.Conclusion The diagnostic model of LN constructed based on the expression level of circRNA in the urine has a high diagnostic efficacy,which has been confirmed by internal tests and may provide strong support for clinicians in the early diagnosis of LN.

Objective To explore the circRNA expression level in the urine of patients with lupus nephritis(LN),to construct a diagnostic model for LN,and to evaluate and verify its diagnostic efficacy.Methods A total of 120 patients with LN admitted from January 2022 to January 2024 were selected as the observation group and divided into the training group(n=84)and the validation group(n=36)at a ratio of 7:3.Another 100 healthy individuals who underwent physical examination were selected as the control group.The expression levels of circRNA-0001445,circRNA-002059,hsa-circ-0007385 and hsa-circ-0000006 in urine specimens of patients in the training group and the control group were detected by gene sequencing.Multivariate Logistic regression analysis was used to construct a diagnostic model for LN based on the urinary circRNA expression level.The diagnostic value of individual indicators and combined predictive models were evaluated by receiver operating characteristic(ROC)curve analysis,and then the predictive efficacy of the model was verified by the validation group.Results The expression levels of circRNA-0001445,circRNA-002059,hsa-circ-0007385 and hsa-circ-0000006 in the urine of patients in the training group were all higher than those of the control group(P<0.01).Multivariate Logistic regression analysis found that The expression levels of circRNA-0001445(OR=1.094),circRNA-002059(OR=1.040),hsa-circ-0007385(OR=1.033),and hsa-circ-0000006(OR=1.130)in theurine were independent factor for the diagnosis of LN(P<0.01).ROC curve analysis showed that the areas under the curve of combined model constructed based on the circRNA expression level in the urine were 0.985 and 0.911 in the training group and the validation group,respectively,with sensitivities of 95.00%and 91.67%,and specificities of 99.00%and 89.00%,all of which were higher than the diagnostic efficacy of a single urinary circRNA expression level.Conclusion The diagnostic model of LN constructed based on the expression level of circRNA in the urine has a high diagnostic efficacy,which has been confirmed by internal tests and may provide strong support for clinicians in the early diagnosis of LN.

Objective:To investigate the influencing factors of adult dental fluorosis in drinking water-borne endemic fluorosis areas of Inner Mongolia Autonomous Region.Methods:A case-control study was conducted in January 2024 to select adult fluorosis patients (case group) and healthy individuals (control group) from the drinking water-borne endemic fluorosis areas in Helinger County, Hohhot City, Inner Mongolia Autonomous Region as the survey subjects. Urine samples were collected to determine urinary fluoride concentration. A questionnaire survey was conducted. SPSS 25.0 software was used for χ 2 test and multivariate logistic regression analysis. Restricted cubic spline (RCS) was used to analyze the association between urinary fluoride concentration and the risk of dental fluorosis in adults. Results:A total of 161 individuals were included in the survey, including 100 in the case group and 61 in the control group. The results of univariate analysis showed that there were statistically significant differences in the distribution of gender, smoking, and urinary fluoride concentration between the case group and the control group (χ 2 = 7.54, 5.02, 9.69, P < 0.05). The results of multivariate logistic regression analysis indicated that gender ( OR = 0.36, 95% CI: 0.18 - 0.73, P = 0.005) and urinary fluoride concentration ( OR = 3.08, 95% CI: 1.46 - 6.67, P = 0.003) were the influencing factors of adult fluorosis. RCS analysis showed a significant linear dose-response relationship between the risk of dental fluorosis and urinary fluoride concentration ( Poverall trend = 0.001, Pnonlinear = 0.071). When the urinary fluoride concentration was greater than 1.57 mg/L, the risk of dental fluorosis increased with the increase of urinary fluoride concentration. Conclusion:Gender and urinary fluoride concentration are the risk factors of dental fluorosis in adults in drinking water-borne endemic fluorosis areas of Inner Mongolia Autonomous Region.

Objective To investigate the effect of embryonic inflammatory exposure on the response of mouse offspring to interphotoreceptor retinoid-binding protein(IRBP)-induced experimental autoimmune uveitis(EAU).Methods RNA transcriptome sequencing data from eyeballs of C57BL/6J mouse offspring born to mothers with active EAU were used to screen immune-associated differentially expressed genes in the eyes of the exposed offspring.Gene fragments overlapping in the two datasets were screened using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses to identify biological pathways associated with the gene fragments.Hub genes were identified from these intersecting genes by protein-protein interaction network analysis.EAU models of maternal uveitis were established by immunization with IRBP651-670,and expression levels of the pivotal genes in the offspring exposed to inflammation by maternal uveitis were examined by fluorescence quantitative polymerase chain reaction.EAU severity,T lymphocyte proliferation,and serum cytokines were detected to investigate the immune effect in offspring from mothers with an active inflammation response to IRBP induction.Results Microarray analysis identified 72 immune-related differentially expressed genes in exposed samples compared with the findings in control samples.These genes were mainly enriched in Toll-like receptor signaling,mitogen-activated protein kinase signaling,and B cell receptor signaling pathways.Protein-protein interaction network interaction analysis screened out four hub genes,Psmc5,Psmc3,Psmd4,and Psmd8,and mRNA levels of these four genes were increased in the adult offspring from mothers with active uveitis compared with the findings in healthy offspring.In addition,the group induced with 150 μg IRBP showed an increase in the severity of clinical and pathological outcomes in offspring with EAU affected by active inflammation,compared with the healthy offspring group(P=0.0087,P=0.0410).Meanwhile,T cell proliferation in the offspring was enhanced during the inflammatory activity stage and secretion of the inflammatory cytokines interleukin(IL)-17 and IL-6 was increased(P=0.0450,P=0.0300).Conclusions Psmc5,Psmc3,Psmd4,and Psmd8 may be important genes exacerbating uveitis in offspring of mothers with active uveitis,associated with increased T cell proliferation and production of IL-17 and IL-6.

OBJECTIVE To establish a rapid analytical method to identify the chemical components in Xitong preparations for the treatment of rheumatoid arthritis by ultra-high performance liquid chromatography coupled triple time-of-flight mass spectrometry(UPLC-Triple-TOF-MS/MS). METHODS The analysis was performed on an ACQUITY UPLC HSS T3 (2.1 mm×150 mm, 1.8 μm) column with the mobile phase consisting of water containing 0.1% formic acid(A) and acetonitrile containing 0.1% formic acid(B) in gradient mode at a flow rate of 0.3 mL·min−1. Data acquisition was carried out under positive and negative ion modes. The processed data was analyzed by Peakview software and compound database. The chemical components were determined by comparison with standard products, combining with the characteristic fragments in secondary MS, and those in the related literatures. RESULTS Seventy-eight compounds were identified including 20 phenylpropanoids, 33 terpenes, 19 flavonoids, 6 oxylipins, two of which might be new compounds. CONCLUSION The method can be used for rapid identification of chemical compositions in Xitong preparations, which provides basis for quality control and elucidation of phamarcodynamic material basis of Xitong pills and capsules .

Objective:To explore the association between different feeding patterns, emotional states, and salivary oxytocin (OT) levels during breastfeeding.Methods:From January to December 2019, 153 pairs of 3-month-old infants and their mothers were recruited from 4 maternal and child health hospitals in Chongqing, Liuzhou, Dalian and Hangzhou in China.Saliva samples were collected from the mothers at the first 5 minutes of feeding, 5 minutes during feeding, and 10 minutes after feeding.Edinburgh postnatal depression scale (EPDS) was used to evaluate maternal depression.Infants were divided into exclusive breastfeeding group and artificial feeding group according to feeding patterns.ELISA of salivary oxytocin was performed by ELISA kits, and the OT levels measured at the 3 time points were converted using linear interpolation.Area under the curve with respect to ground(OTAUCG) was used to represent the total concentration of salivary OT during the mother's breastfeeding.SPSS 25.0 software was used for statistical analysis.Multiple linear regression analysis and two factors analysis of variance were used to explore the association between different feeding methods, emotional state and salivary oxytocin during breastfeeding.Results:The results of the two factors analysis of variance showed that the interaction between feeding pattern and mother's emotion was not significant ( F=2.440, P=0.120), the main effect of mother's emotion was not significant ( F=0.380, P=0.539), and the main effect of feeding style was significant ( F=3.350, P=0.021). The level of OTAUCG under pure breastfeeding ((151 561.47±75 738.11) pg/mL) was higher than that under artificial feeding ((122 269.03±65 029.88) pg/mL), and the difference was statistically significant ( P=0.02). There was no statistically significant difference in OTAUCG levels between mothers with normal emotions ((146 106.37±75 106.76) pg/mL) and mothers with depressed emotions ((129 079.56±67 565.87) pg/mL) ( P=0.221). Multiple stepwise regression analysis showed that artificial feeding had a negative predictive effect on maternal salivary OT levels compared to exclusive breastfeeding( β=-0.211, t=-2.513, P=0.013). Conclusion:Feeding pattern is a factor that affects the mother's salivary OT level, and breastfeeding can improve the mother's OT level.

Objective:Analyze the correlation between serum immunoglobulin G (IgG) N-glycan and Lauren classification of gastric cancer.Methods:A retrospective study was performed on 17 patients with diffuse type gastric cancer and 21 patients with intestinal type who received treatment in Zhongshan Hospital from 2017 to 2018, and the general medical history data and disease characteristics were summarized. The serum IgG glycome profiles were analyzed by ultraperformance liquid chromatography, and the difference between intestinal type and diffuse type gastric cance was compared.Logistic regression was used to evaluate the correlation between serum IgG N-glycan and Lauren classification.Results:IgG N-glycome analysis included 27 directly detected glycans and 4 derived traits. H=Hexose, N=N-acetylglucosamine, F=Fucose, S=Sialic acid.There was no significant difference in IgG N-glycan among different chemotherapy protocol. Compared with intestinal type, H3N3F1 ( t=3.785, P=0.001), H3N4( t=3.919, P=0.002), H3N4F1( t=2.770, P=0.005), H3N5F1( t=2.888, P=0.010) were decreased in diffuse type; H4N4F1(6)( t=?3.488, P<0.001), H5N4F1( t=?3.401, P=0.003), H5N5F1( t=?2.303, P=0.023), H5N4F1S1 ( t=?3.068, P=0.008) were increased.H3N3F1( OR:1.20, P=0.008), H3N4( OR:1.32, P=0.005), H3N4F1 ( OR:1.13, P=0.017), H3N5F1 ( OR:1.78, P=0.015), H4N4F1(6)( OR:0.43, P=0.008), H5N4F1(6)( OR:0.74, P=0.008), H5N5F1 ( OR:0.32, P=0.036), H5N4F1S1( OR:0.48, P=0.009) were significantly correlated with Lauren classification. Sialylated ( t=?2.717, P=0.012) and galactosylated ( t=?3.400, P=0.001) IgG N-glycan were reduced in patients with intestinal type gastric cancer.Galactosylated ( OR:0.87, P=0.007) and sialylated ( OR:0.62, P=0.015) IgG N-glycan were significantly correlated with Lauren classification. Conclusion:Some IgG N-glycan are significantly correlated with Lauren classification, which can be used as potential biomarkers.