Objective To determine the chemical composition of TangNiaoLing Tablets by UHPLC-Q-Exactive-Orbitrap-MS/MS.Methods A Waters ACQUITY HSS T3 column(100 mm×2.1 mm,1.8 μm)was used for separation at a total flow rate of 0.2 mL/min.The mobile phase included an aqueous solution of 0.1%formic acid and acetonitrile mixed with 0.1%formic acid was supplied.The injection volume was set at 2 μL and the column oven temperature was 40℃.High-resolution mass spectrometric data were obtained by concurrently scanning the positive and negative ion modes.The identification was accomplished by inferring the empirical fragmentation patterns and comparing it with databases and references.Results 100 different chemical elements,including triterpenes,flavonoids,phenylpropanoids,phenylethanoid glycosides,iridoid glycosides,and phenols,among others were identified from the 50%methanol extract of TangNiaoLing pills.Conclusion The chemical contents of TangNiaoLing tablets were identified and analyzed using the UHPLC-Q-Exactive-Orbitrap-MS/MS method for the first time.This served as a foundation for future research into the tablets' effective components and quality control.

Objective To determine the chemical composition of TangNiaoLing Tablets by UHPLC-Q-Exactive-Orbitrap-MS/MS.Methods A Waters ACQUITY HSS T3 column(100 mm×2.1 mm,1.8 μm)was used for separation at a total flow rate of 0.2 mL/min.The mobile phase included an aqueous solution of 0.1%formic acid and acetonitrile mixed with 0.1%formic acid was supplied.The injection volume was set at 2 μL and the column oven temperature was 40℃.High-resolution mass spectrometric data were obtained by concurrently scanning the positive and negative ion modes.The identification was accomplished by inferring the empirical fragmentation patterns and comparing it with databases and references.Results 100 different chemical elements,including triterpenes,flavonoids,phenylpropanoids,phenylethanoid glycosides,iridoid glycosides,and phenols,among others were identified from the 50%methanol extract of TangNiaoLing pills.Conclusion The chemical contents of TangNiaoLing tablets were identified and analyzed using the UHPLC-Q-Exactive-Orbitrap-MS/MS method for the first time.This served as a foundation for future research into the tablets' effective components and quality control.