Objective To investigate the effect and mechanism of endothelin-1(ET-1)on atrial fibrosis in Atrial fibrillation(AF)rats.Methods Fourteen adult male SD rats were randomly divided into normal control(NC)group and Atrial fibrillation(AF)group.The rat model of Atrial fibrillation was established by injecting 0.1 ml/100g CaCl2-Ach mixture into the tail vein once a day for one week.The control group was injected with the same dose of normal saline.An electrocardiogram of normal or atrial fibrillation was recorded on the first day and the eighth day in each group,and echocardiography was used to monitor atrial size and cardiac function.The fibrosis of atrial was observed using Masson and HE staining.The expression of endothelin-1(ET-1),collagen-I(Col-I),transforming growth factor-β(TGF-β)and the store operated calcium channel(SOCC)protein Orai1,stromal in-teraction molecule 1(STIM1)in atrial tissue were detected by Western blot.HL-1 cells were cultured and treated with gradient concentration of ET-1 for 24 hours.Western blot was used to observe changes in the expression of TGF-β,Orai1 and STIM1 proteins in ET-1/SOCC/TGF-β signaling pathway of HL-1 cells.Small interfering RNA(siRNA)transfection method was used to knock down the expression of Orai1 in HL-1 cells,then the cells were treated with appropriate concentrations of ET-1 for 24 hours,and the expression of TGF-β protein in HL-1 cells was detected by Western blot.Results Compared with the control group,echocardiography showed a significant in-crease in left atrial diameter(LAD)of the heart in atrial fibrillation rats(P<0.05).The HE and Masson staining results showed significant fibrosis in the myocardial tissue of AF group rats(P<0.05),and the Western blot re-sults indicated the expression of ET-1,Orai1,STIM1,TGF-β and COL-Ⅰ in the myocardial tissue of AF group significantly increased compared to the NC group(P<0.05).After ET-1 treatment of HL-1 cells,the protein ex-pression of Orai1,STIM1and TGF-β increased(P<0.05),while knocking down Orai1 in HL-1 cells,ET-1 treat-ment no longer caused the expression of TGF-β a significant upregulation.Conclusion AF caused by atrial fibril-lation results in a significant increase in ET-1 expression in atrial tissue,and ET-1/SOCC/TGF-β signal pathway promotes atrial fibrillation and fibrosis.

Objective:To identify the co-expressed different expressed genes (DEGs) related to long-term responding in patients receiving vedolizumab or etrolizumab, whose expression could be tested to indicate the treatment efficacy when taking anti-α4β7 integrins.Methods:Two datasets were downloaded from Gene Expression Omnibus (GEO) to be analyzed. Limma, Gene Ontology (GO) and KEGG analysis were used to screen DEGs and possible pathways. The most significant module in the PPI networks was analyzed by Cytoscape. ROC curves were built to indicate the predictive potential.Results:A total of 433 DEGs were found between patients′ response and non-response to vedolizumab, and 15 DEGs between patients received vedolizumab and etrolizumab. The GO and KEGG analyses showed overlaps were enriched to some immune-related pathways closely matched to the mechanism of anti-integrins. Eight hub genes were distinguished from the PPI network: MNDA, AQP9, FPR1, FCGR3B, VNN2, TREM1, MYO1F and GAS2L3 (5 of them had been identified to be related to IBD in labs) . Conclusions:Efficacy of anti-α4β7 intergins is closely associated with inflammation-related and adhesion-related genes. The possible hub genes and related pathways are distinguished, which can be used as potential long-term response indicators towards suitable patients receiving anti-integrins.

Objective:To identify the co-expressed different expressed genes (DEGs) related to long-term responding in patients receiving vedolizumab or etrolizumab, whose expression could be tested to indicate the treatment efficacy when taking anti-α4β7 integrins.Methods:Two datasets were downloaded from Gene Expression Omnibus (GEO) to be analyzed. Limma, Gene Ontology (GO) and KEGG analysis were used to screen DEGs and possible pathways. The most significant module in the PPI networks was analyzed by Cytoscape. ROC curves were built to indicate the predictive potential.Results:A total of 433 DEGs were found between patients′ response and non-response to vedolizumab, and 15 DEGs between patients received vedolizumab and etrolizumab. The GO and KEGG analyses showed overlaps were enriched to some immune-related pathways closely matched to the mechanism of anti-integrins. Eight hub genes were distinguished from the PPI network: MNDA, AQP9, FPR1, FCGR3B, VNN2, TREM1, MYO1F and GAS2L3 (5 of them had been identified to be related to IBD in labs) . Conclusions:Efficacy of anti-α4β7 intergins is closely associated with inflammation-related and adhesion-related genes. The possible hub genes and related pathways are distinguished, which can be used as potential long-term response indicators towards suitable patients receiving anti-integrins.

Objective To explore the value of elastography score and strain rate ratio in the diagnosis of small breast malignant focus. Methods We retrospectively analyzed 22 patients with breast small malignant foci less than 10 mm. Ultrasound characteristics were summed up in breast small cancer. Results On elastogram, 2 patients scored 3, 14 scored 4 and 6 scored 5.The average strain rate ratio of all foci was 4.76, and there was correlation between it and elastography scores. Conclusion Ultrasonic elastography has important value in the diagnosis of breast small cancer.