Objective:To study the effect of inhibiting heat shock protein 70 ( HSP70) gene expression on the proliferation, invasion and migration of cholangiocarcinoma cells and its mechanism. Methods:Tumor tissues and adjacent normal tissue samples from 23 patients with cholangiocarcinoma who underwent surgery in the Hunan Second People′s Hospital from January 2022 to June 2023 were collected. The mRNA and protein expressions of HSP70 in cholangiocarcinoma tissues, human cholangiocarcinoma cells (HuCC-T1), normal bile duct tissues and human intrahepatic biliary epithelial cells (HIBEpiC) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. HuCC-T1 cells were cultured in vitro, and a HuCC-T1 cell line with stably knocked down HSP70 gene (HuCC-T1-HSP70-KD group) was obtained by screening after infection with shRNA lentivirus. Cell counting kit-8 (CCK-8) assay and Transwell assay were used to detect the effects of inhibiting HSP70 gene expression on the proliferation, invasion and migration abilities of HuCC-T1 cells. Western blot was used to detect the protein expressions of Toll-like receptor (TLR) 4, phosphorylated extracellular regulated protein kinases 1/2 (p-ERK1/2), ERK1/2, β-catenin, c-myc, Snail and E-cadherin after inhibiting HSP70 gene expression in HuCC-T1 cells. Results:Compared with normal bile duct tissues and HIBEpiC cells, the mRNA and protein expressions of HSP70 in cholangiocarcinoma tissues and HuCC-T1 cells were significantly higher (all P<0.05). After inhibiting HSP70 gene expression in HuCC-T1 cells, the proliferation, invasion and migration abilities of cells in the HuCC-T1-HSP70-KD group were significantly decreased (all P<0.05); the protein expressions of TLR4, p-ERK1/2, β-catenin, c-myc and Snail in the HuCC-T1-HSP70-KD group were significantly decreased, while the protein expression of E-cadherin was significantly increased (all P<0.05). Conclusions:Silencing HSP70 gene expression can significantly inhibit the proliferation, invasion and migration abilities of cholangiocarcinoma cells. The mechanism may be that after the down-regulation of HSP70 gene expression, its activation of downstream TLR4 and MAPK pathways is significantly inhibited, thereby affecting the proliferation, invasion and migration of cholangiocarcinoma cells.

Objective:To study the effect of inhibiting heat shock protein 70 ( HSP70) gene expression on the proliferation, invasion and migration of cholangiocarcinoma cells and its mechanism. Methods:Tumor tissues and adjacent normal tissue samples from 23 patients with cholangiocarcinoma who underwent surgery in the Hunan Second People′s Hospital from January 2022 to June 2023 were collected. The mRNA and protein expressions of HSP70 in cholangiocarcinoma tissues, human cholangiocarcinoma cells (HuCC-T1), normal bile duct tissues and human intrahepatic biliary epithelial cells (HIBEpiC) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. HuCC-T1 cells were cultured in vitro, and a HuCC-T1 cell line with stably knocked down HSP70 gene (HuCC-T1-HSP70-KD group) was obtained by screening after infection with shRNA lentivirus. Cell counting kit-8 (CCK-8) assay and Transwell assay were used to detect the effects of inhibiting HSP70 gene expression on the proliferation, invasion and migration abilities of HuCC-T1 cells. Western blot was used to detect the protein expressions of Toll-like receptor (TLR) 4, phosphorylated extracellular regulated protein kinases 1/2 (p-ERK1/2), ERK1/2, β-catenin, c-myc, Snail and E-cadherin after inhibiting HSP70 gene expression in HuCC-T1 cells. Results:Compared with normal bile duct tissues and HIBEpiC cells, the mRNA and protein expressions of HSP70 in cholangiocarcinoma tissues and HuCC-T1 cells were significantly higher (all P<0.05). After inhibiting HSP70 gene expression in HuCC-T1 cells, the proliferation, invasion and migration abilities of cells in the HuCC-T1-HSP70-KD group were significantly decreased (all P<0.05); the protein expressions of TLR4, p-ERK1/2, β-catenin, c-myc and Snail in the HuCC-T1-HSP70-KD group were significantly decreased, while the protein expression of E-cadherin was significantly increased (all P<0.05). Conclusions:Silencing HSP70 gene expression can significantly inhibit the proliferation, invasion and migration abilities of cholangiocarcinoma cells. The mechanism may be that after the down-regulation of HSP70 gene expression, its activation of downstream TLR4 and MAPK pathways is significantly inhibited, thereby affecting the proliferation, invasion and migration of cholangiocarcinoma cells.

Objective To investigate the clinical value of laryngeal mask airway (LMA) in patients with a-cute severe asthma(ASA). Methods 32 patients with ASA treated with LIMA or mouth-nose mask during 2002 -2009 in our hospital were retrospectively analyzed. Those treated with laryngeal mask airway was taken as observation group and those with Mouth-nose mask as control group. Results The period to oxygen saturation in arterial blood, the time to remove ventilator, and the time to disease improvement in the observation group (389.63±32.82)s, (19.31±2.26) hours,(16.22±3.85) hours were different from that in control group (467.36±41.15) s, (25.18±3.73) hours,(23.66±2.38) hours (P<0.01). After non-invasive positive pressure ventilation, PaCO_2 decreased, PaO_2 and pH increased at 3 and 12 hours in the observation group (P<0.05 or 0.01) from that before treatment. PaCO_2 and pH at 3 hours in the control group were no significant difference before and after treatment (P > 0.05),with an exception of PaO_2 (P < 0.05). PaCO_2, PaO_2 and pH were significantly different (P < 0.05) at 12 hours after treatment from those before treatment. Conclusions LMA should be considered in the selection of non-invasive positive-pressure ventilation (NIPPV) in patients with ASA, for a better improvement of ventilation ef-fectivenoss and accelerating the mitigation of clinical manifestations.