Innate nucleic acid sensing is a ubiquitous and highly conserved immunological process,which is pivotal for monitoring and responding to pathogenic invasion and cellular damage,and central to host defense,autoimmunity,cell fate determination and tumorigenesis.Tyrosine phosphorylation,a major type of post-translational modification,plays a critical regulatory role in innate immune sensing pathway.Core members of nucleic acid sensing signaling pathway,such as cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS),stimulator of interferon genes(STING),and TANK binding kinase 1(TBK1),are all subject to activity regulation triggered by tyrosine phosphorylation,thereby affecting the host antiviral defense and anti-tumor immunity under physiological or pathological conditions.This review summarizes the recent advances in research on tyrosine kinases and tyrosine phosphorylation in regulation of nucleic acid sensing.The function and potential applications of targeting tyrosine phosphorylation in anti-tumor immunity is disussed to provide insights for understanding and expanding new anti-tumor strategies.