Dengue fever is an arboviral infectious disease caused by the dengue virus(DENV),primarily transmitted by Aedes aegypti and Aedes albopictus mosquitoes.In recent years,due to factors such as climate change,accelerated urbanization and frequent international travel,this has led to a significant increase in new cases and mortality rates.The early symptoms of dengue fever lack specificity,which leads to be difficult for early diagnosis.At present,there is no specific anti-DENV drug,and in clinical practice,the supportive therapy is the main approach.This article reviews the latest epidemiological situation,biomarker screening,antiviral drug study and development,and prevention and control strategies for dengue fever.It emphasizes the impor-tance of identifying the effective biomarkers and accelerating the study and development of antiviral drugs in order to provide the scientific evidence and technical support for more effective prevention and control meas-ures to address the public health challenges posed by dengue fever.

Objective:To evaluate the effect of hyperthermia on radiation pneumonitis (RP) in elderly patients with esophageal cancer undergoing intensity-modulated radiotherapy (IMRT).Methods:Clinical data of 177 elderly esophageal cancer patients (aged ≥60 years) receiving IMRT in the First Affiliated Hospital of Soochow University and Yixing Cancer Hospital from August 1, 2017 to February 6, 2023 were retrospectively analyzed. Patients were divided into the hyperthermia and non-hyperthermia groups based on whether they received hyperthermia treatment. Patients in two groups received IMRT with 6 MV X-rays. Patients in the hyperthermia group underwent high-frequency hyperthermia within 1 h before radiation using the external thermotherapy device HG-2000Ⅲ (heating temperature: 41-43 ℃ for 40 min, twice a week). After adjusting for confounding factors between two groups using propensity score matching (PSM), the short-term effective rates between two groups were compared using Chi-square test. Univariate analysis and logistic multivariate analysis were employed to compare the incidence of RP between two groups. Results:After applying PSM, 42 pairs were successfully matched, and the baseline data and radiotherapy parameters showed no statistically significant differences between two groups (all P>0.05). The objective response rate (ORR) in the hyperthermia group was significantly higher than that in the non-hyperthermia group (83.3% vs. 64.3%, P=0.047). Univariate analysis revealed that the incidence of RP and symptomatic RP (≥ grade 2) in the hyperthermia group was significantly lower than that in the non-hyperthermia group (61.9% vs. 85.7%, P=0.013; 21.4% vs. 47.6%, P=0.012). Logistic multivariate analysis indicated that hyperthermia was an independent protective factor for symptomatic RP ( P=0.011). Conclusions:The incidence and severity of RP in elderly esophageal cancer patients receiving IMRT can be reduced by hyperthermia. Hyperthermia, as a clinically beneficial green treatment, improves efficacy and reduces toxicity for patients with esophageal cancer.

Objective:To automatically classify hypertensive heart disease (HHD) and hypertrophic cardiomyopathy (HCM) based on mul-titask learning algorithm using native T1 mapping images.Methods:A total of 203 patients admitted to Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University from January 2017 to December 2021 were enrolled, including 53 patients with HHD, 121 patients with HCM, and 29 patients with normal control (NC). Native T1 mapping images of all enrolled patients were acquired using MRI and processed by a multi-task learning algorithm. The classification performance of each model was validated using ten-fold crossover, confusion matrix, and receiver operator characteristic (ROC) curves. The Resnet 50 model based on the original images was established as a control.Results:The ten-fold crossover validation results showed that the MTL-1 024, MTL-64, and MTL-all models showed better performance in terms of area under the curve (AUC), accuracy, sensitivity, and specificity compared to the Resnet 50 model. In the classification task, the MTL-64 model showed the best performance in terms of AUC (0.942 1), while the MTL-all model reached the highest value in terms of accuracy (0.852 2). In the segmentation task, the MTL-64 model achieved the best results with the Dice coefficient (0.879 7). The confusion matrix plot showed that the MTL model outperforms the Resnet 50 model based on the original image in terms of overall performance. The ROC graphs of all MTL models were significantly higher than the original image input Resnet 50 model.Conclusions:Multi-task learning-based native T1 mapping images are effective for automatic classification of HHD and HCM.

Objective To investigate the correlations between acceptance of disability,coping style and depression level in patients with hemiplegia after stroke.Methods A total of 96 patients with hemiplegia after stroke were selected from No.904 Hospital of Joint Logistic Support Force of PLA from May 2019 to June 2022.Acceptance of disability scale-revised(ADS-R),medical coping modes questionnaire(MCMQ)and self-rating depression scale(SDS)were used to investigate disability acceptance,coping style and depression degree in these patients,respectively.Pearson correlation analysis was used to analyze their correlations.Results There were significant differences in ADS-R scores among patients with different numbers of organs with dysfunction,numbness of limbs,ages,and occupations(P<0.05).There were 29 patients with low acceptance of disability,52 patients with moderate acceptance,and 15 patients with high acceptance.The scores of yielding coping,avoidance coping,and SDS in patients with high acceptance of disability were significantly lower than those in patients with low and moderate acceptance of disability(P<0.05),and the scores of yielding coping,avoidance coping,and SDS in patients with moderate acceptance of disability were significantly lower than those in patients with low acceptance(P<0.05).The patients with high acceptance of disability had significantly higher scores of face coping than those with low acceptance and moderate acceptance(P<0.05),and the patients with moderate acceptance had significantly higher scores of face coping than those with low acceptance(P<0.05).Pearson correlation analysis showed that ADS-R score was negatively correlated with yielding coping score,avoidance coping score,and SDS score(P<0.05),while ADS-R score was positively correlated with face coping score(P<0.05).Yielding coping score and avoidance coping score were positively correlated with SDS score(P<0.05),and face coping score was negatively correlated with SDS score(P<0.05).Conclusion The higher the ADS-R score of hemiplegia patients after stroke,the lower the yielding coping score,avoidance coping score and SDS score,and the higher the face coping score.Clinical intervention should be taken to increase ADS-R score and improve patients'coping style and depression degree.

Objective:To investigate the clinical features and laboratory examination results of complete Kawasaki disease (CKD) versus incomplete Kawasaki disease (IKD). Methods:The clinical data of children with complete Kawasaki disease (CKD group, n = 217) and incomplete Kawasaki disease (IKD group, n = 103) who received treatment in Gansu Provincial Maternity and Child-care Hospital from January 2014 to December 2018 were retrospectively analyzed. Clinical symptom features and laboratory examination indexes were compared between the two groups. Results:The incidence of fever in both groups was 100.0%, but the fever time in the IKD group was (8.97 ± 1.76) days, which was significantly longer than (6.60 ± 1.01) days in the CKD group ( t = 7.68, P < 0.05). The incidences of conjunctival hyperemia, chapped lips, bayberry tongue and finger sclerosis and erythema in the IKD group were 82.5% (84/103), 66.9% (69/103), 21.4% (22/103), and 23.3% (24/103), which were significantly lower than 94.9% (206/217), 76.9% (167/217), 75.1% (163/217), and 81.1% (176/217) in the CKD group ( χ2 = 14.71, 7.09, 82.76, 99.58, all P < 0.05). The incidences of polymorphic rash and perianal peeling in the IKD group were 76.7% (79/103) and 33.9% (35/103), respectively, which were significantly higher than 64.9% (141/217) and 23.5% (51/217) in the CKD group ( χ2 = 4.47, 3.90, both P < 0.05). Digestive and respiratory symptoms were more common in the IKD group than in the CKD group ( P < 0.05). C-reactive protein level in the IKD group was (67.56 ± 23.35) mg/L, which was significantly higher than (53.91 ± 25.06) mg/L in the CKD group ( t = 2.46, P < 0.05), while white blood cell count, platelet count, and B-type brain natriuretic peptide level in the IKD group were significantly lower than those in the CKD group ( t = 2.00, 2.34, 4.69, all P < 0.05). The incidences of coronary artery dilation/small coronary artery aneurysm, and pericardial effusion in the IKD group were greater than those in the CKD group ( χ2 = 6.70, 12.87, both P < 0.05). Conclusion:Children with IKD have a long time of fever. In children without obvious clinical features, attention should be paid to the differential diagnosis of IKD from CKD. IKD should be diagnosed and treated as early as possible to decrease the incidence of coronary artery disease.

Objective:To analyze the prognostic factors affecting salvage liver transplantation (SLT).Methods:The clinical data of 97 patients undergoing liver transplantation in the Ningbo Medical Centre Lihuili Hospital from January 2012 to May 2022 were retrospectively analyzed, including 84 males and 13 females, aged (53.6±7.4) years. Among them, 33 patients underwent primary liver transplantation (PLT) and 64 underwent SLT. SLT patients were subdivided into the groups within the Milan criteria (SLT-A, n=35) and beyond the Milan criteria (SLT-B, n=29), according to whether the Milan criteria were met at first hepatectomy. Clinicopathological and prognostic data were subsequently analyzed. Results:The tumor number ( χ2=16.03, P<0.001), microvascular invasion (MVI) ( χ2=10.97, P=0.004), recurrence rate ( χ2=9.31, P=0.010), recurrence-free survival (RFS, F=14.05, P=0.001) and overall survival (OS, F=17.27, P<0.001) were significantly different among the three groups. RFS ( P=0.047) and OS ( P=0.012) in PLT group were better than those in SLT-B group. RFS ( P=0.007) and OS ( P=0.024) in SLT-A group were also better than those in SLT-B group. The multivariate analysis indicated that beyond the Milan criteria at first hepatectomy was an independent risk factor for RFS ( HR=4.378, 95% CI: 1.393-13.756, P=0.011) and OS ( HR=5.391, 95% CI: 1.428-20.352, P=0.013) in patients undergoing SLT, and MVI positive ( HR=4.042, 95% CI: 1.137-14.368, P=0.031) was an independent risk factor for RFS in patients undergoing SLT. Conclusion:Patients beyond the Milan criteria at first hepatectomy and MVI positive showed a poorer prognosis after SLT. Whether the Milan criteria should be the gold standard for SLT as well as for PLT needs further study.

Objective:To design a lung function prediction method that combines transfer learning and multimodal feature fusion, aiming to improve the accuracy of lung function prediction in patients with idiopathic pulmonary fibrosis (IPF).Methods:CT images and clinical text data were reprocessed, and an adaptive module was designed to find the most suitable lung function attenuation function for IPF patients. The feature extraction module was utilized to comprehensively extract features. The feature extraction module comprises three sub-modules, including CT feature extraction, clinical text feature extraction, and lung function feature extraction. A multimodal feature prediction network was used to comprehensively evaluate the attenuation of lung function. The pre-trained model was fine-tuned to improve the predictive performance of the model.Results:Based on the OSIC pulmonary fibrosis progression competition dataset, it is found through the adaptive module that the linear attenuation hypothesis is more in line with the trend of pulmonary function decline in patients. Different modal data prediction experiments show that the model incorporating clinical text features has better predictive ability than the model using only CT images. The model combining CT images, clinical text features, and lung function features have optimal predictive results. The lung function prediction method combining transfer learning and multimodal feature fusion has modified version of the Laplace log likelihood (LLLm) of ?6.706 5, root mean squared error (RMSE) of 184.5, and mean absolute error (MAE) of 146.2, which outperforms other methods in terms of performance. The pre-trained model has higher prediction accuracy compared to the zero base training model.Conclusions:The lung function prediction method designed by combining transfer learning and multimodal feature fusion can effectively predict the lung function status of IPF patients at different weeks, providing important support for patient health management and disease diagnosis.

Objective:To observe the efficacy of minocycline combined with barrier cream containing prickly thorn fruit oil and acid slurry extract in the treatment of patients with erythematous telangiectasia or papular pustular rosacea.Methods:From August 2020 to April 2021, 44 patients with rosacea were admitted to the Department of Dermatology, the Ninth People′s Hospital, including 28 males and 16 females, aged 20-56 (37±7) years. They were randomly divided into the observation group (minocycline plus barrier cream) and the control group (minocycline plus vaseline ointment), with 22 patients in each group.Results:After 8 weeks of medication, 21 cases (95.5%) in the observation group were effective, while 16 cases (72.7%) in the control group were effective. The efficacy of the observation group was better than that of the control group, the difference was statistically significant (χ 2=2.06, P=0.039). No significant adverse reactions were observed in both groups. Conclusions:Minocycline combined with barrier cream containing prickly thorn oil and acid slurry extract has a good therapeutic effect on rosacea, which is worthy popularizing.

Objective:To investigate the correlation between the prognosis of late-onset depression(LOD)in the elderly and lncRNA expression levels and coping styles.Methods:Differential expression of lncRNAs in peripheral blood of LOD 92 patients was detected by a real-time quantitative PCR(qPCR)detection system, and the Hamilton Depression Scale(HAMD)and the Trait Coping Style Questionnaire(TCSQ)were used for psychological assessment.Results:Compared with the control group, the expression levels of TCONS_00019174(7.55 vs.4.36), ENST00000566208(6.48 vs.3.26), ENST00000517573(8.33 vs.5.32)and NONHSAT142707(6.78 vs.3.26)in elderly patients of the LOD group were significantly down-regulated( Z=5.09, 5.87, 4.35, 6.44, P<0.05); Compared with the low-expression subgroup, scores of anxiety/somatization[(3.83±1.40) vs.(6.39±2.35)], diurnal variation[(0.22±0.42) vs.(0.83±0.94)], retardation[(5.74±0.96) vs.(6.48±1.28)], hopelessness[(2.78±0.67) vs.(4.52±1.56)]and HAMD[(20.39±1.75) vs.(26.83±4.88)]in the high-expression subgroup were significantly lower( t=-4.50, -2.84, -2.22, -4.90, -5.96, P<0.05). The ΔCT value of TCONS_00019174 was negatively correlated with the reduction rates of anxiety/somatization, diurnal variation, retardation, sleep disturbance, hopelessness and HAMD( r=-0.40-0.66, P<0.05). The ΔCT value of ENST00000566208 was negatively correlated with the reduction rates of anxiety/somatization, sleep disturbance, hopelessness and HAMD( r=-0.47-0.62, P<0.01). The ΔCT values of ENST00000517573, NONHSAT034045 and NONHSAT142707 were negatively correlated with the reduction rates of retardation, sleep disturbance, hopelessness and HAMD( r=-0.39-0.76, P<0.05). The positive coping style was positively correlated with the reduction rates of HAMD, anxiety/somatization, retardation, sleep disturbance and hopelessness( r=0.38-0.55), while the negative coping style was negatively correlated with the reduction rates of HAMD, anxiety/somatization, sleep disturbance and hopelessness( r=-0.39-0.67, P<0.05). When TCONS_00019174, ENST00000566208, NONHSAT034045, NONHSAT142707, positive coping and negative coping were taken into the regression equation as variables for HAMD reduction, it was found that they were able to explain 32.4% of the variance for the reduction rate of the total HAMD score( t=-8.713, -3.584, -3.864, -2.257, 5.675, -2.357, P<0.05). Conclusions:TCONS_00019174, ENST00000566208, NONHSAT034045, NONHSAT142707, positive coping style and negative coping style are predictors of the prognosis of LOD in the elderly.

Objective:To establish neurodevelopmental mice model of schizophrenia(SZ) with prepulse inhibition(PPI) deficits and investigate the effectiveness of olanzapine on PPI disruption.Methods:On the 9th day of pregnancy of SPF grade C57BL/6 mice, female mice were injected with polyinosinic acid poly (I∶C) (6 mg/kg) through tail vein for immune stimulation. The stress model was constructed by chronic unpredictable mild stress 30-40 d after birth (PND30-40). The offspring mice were divided into pregnancy immune stimulation + adolescent stress group (P + S + group), pregnancy immune stimulation group (P + S- group), adolescent stress group (P-S+ group) and non stimulation group (P-S-group), with 18 mice in each group. The mice in P+ S+ group were divided into OLZ intervention group (OLZ group) and non-OLZ intervention group (non-OLZ group), with 9 mice in each group. The PPI function of mice was detected by acoustic startle reflex test after modeling and OLZ intervention. Adopt StatView Version 5.0 software for data analysis, and multi factors analysis of variance was used to test the main effect, interactive effect and simple effect of each factor.Results:The main effects of maternal Poly(I: C) immune activation and pubertal chronic unpredictable stress were significant( F(1, 330)=47.72, P<0.01), and there was a significant interaction between the two factors( F(1, 330)=14.80, P<0.01), simple effect analysis showed that average percent prepulse inhibition (PPI%) in P+ S+ group((15.42±6.13)%) was significantly decreased compared with groups of P+ S-((27.33±4.58)%), P-S+ ((31.17±3.97)%) and P-S-((47.14±12.28)%)(all P<0.01). There was significant gender difference in Prepulse inhibition(PPI)score( F(1, 396)=61.94, P<0.01), in male and female mice, average startle reactivity of Pulse under Prepulse+ Pulse influence of distinct intensities was significantly different( F(1, 198)=18.68, 18.44, P<0.01), and the maternal Poly(I∶C) immune activation had a significant main effect( F(1, 198)=32.18, 12.58, P<0.01) and interaction with pubertal chronic unpredictable stress( F(1, 198)=34.54, 11.39, P<0.01), simple effect analysis suggested that the average startle reactivity of Prepulse+ Pulse in P+ S+ group(0.47±0.12) was significantly higher than other three groups(P+ S-: 0.36±0.11, P-S+ : (0.25±0.22), P-S-: (0.31±0.19)) in male mice( P<0.01) and in P-S+ group was significantly higher than the other three groups in female mice ( P<0.01). OLZ treatment could efficiently reverse the deficits on PPI by increasing PPI%( F(1, 165)=18.24, P<0.01), OLZ could reduce PPI score in male "dual-hit" model mice( F(1, 102)=21.81, P<0.01)and raise it in female( F(1, 102)=4.88, P<0.05). Conclusion:OLZ can reverse PPI deficits in schizophrenic neurodevelopmental model mice, and in the evaluation of PPI function in the model mice through PPI of acoustic startle reflex, PPI% has better stability and reactivity to OLZ intervention.