PURPOSE: To investigate the incidence and influencing factors of bone loss in patients with spinal cord injury (SCI). METHODS: A retrospective case-control study was conducted. Patients with SCI in our hospital from January 2019 to March 2023 were collected. According to the correlation between bone mineral density (BMD) at different sites, the patients were divided into the lumbar spine group and the hip joint group. According to the BMD value, the patients were divided into the normal bone mass group (t > -1.0 standard deviation) and the osteopenia group (t ≤ -1.0 standard deviation). The influencing factors accumulated as follows: gender, age, height, weight, cause of injury, injury segment, injury degree, time after injury, start time of rehabilitation, motor score, sensory score, spasticity, serum value of alkaline phosphatase, calcium, and phosphorus. The trend chart was drawn and the influencing factors were analyzed. SPSS 26.0 was used for statistical analysis. Correlation analysis was used to test the correlation between the BMD values of the lumbar spine and bilateral hips. Binary logistic regression analysis was used to explore the influencing factors of osteoporosis after SCI. p < 0.05 was considered statistically significant. RESULTS: The incidence of bone loss in patients with SCI was 66.3%. There was a low concordance between bone loss in the lumbar spine and the hip, and the hip was particularly susceptible to bone loss after SCI, with an upward trend in incidence (36% - 82%). In this study, patients with SCI were divided into the lumbar spine group (n = 100) and the hip group (n = 185) according to the BMD values of different sites. Then, the lumbar spine group was divided into the normal bone mass group (n = 53) and the osteopenia group (n = 47); the hip joint group was divided into the normal bone mass group (n = 83) and the osteopenia group (n = 102). Of these, lumbar bone loss after SCI is correlated with gender and weight (p = 0.032 and < 0.001, respectively), and hip bone loss is correlated with gender, height, weight, and time since injury (p < 0.001, p = 0.015, 0.009, and 0.012, respectively). CONCLUSIONS The incidence of bone loss after SCI was high, especially in the hip. The incidence and influencing factors of bone loss in the lumbar spine and hip were different. Patients with SCI who are male, low height, lightweight, and long time after injury were more likely to have bone loss.
Humans ; Spinal Cord Injuries/complications* ; Male ; Female ; Retrospective Studies ; Incidence ; Adult ; Bone Density ; Middle Aged ; Case-Control Studies ; Osteoporosis/etiology* ; Lumbar Vertebrae ; Bone Diseases, Metabolic/etiology* ; Aged ; Risk Factors

Ureaplasma urealyticum (UU) is one of the most commonly occurring pathogens associated with genital tract infections in infertile males, but the impact of seminal UU infection in semen on intrauterine insemination (IUI) outcomes is poorly understood. We collected data from 245 infertile couples who underwent IUI at The First Affiliated Hospital of USTC (Hefei, China) between January 2021 and January 2023. The subjects were classified into two groups according to their UU infection status: the UU-positive group and the UU-negative group. We compared semen parameters, pregnancy outcomes, and neonatal birth outcomes to investigate the impact of UU infection on IUI outcomes. There were no significantly statistical differences in various semen parameters, including semen volume, sperm concentration, total and progressive motility, sperm morphology, leukocyte count, the presence of anti-sperm antibody, and sperm DNA fragmentation index (DFI), between the UU-positive and UU-negative groups of male infertile patients (all P > 0.05). However, the high DNA stainability (HDS) status of sperm differed between the UU-positive and UU-negative groups, suggesting that seminal UU infection may affect sperm nuclear maturation ( P = 0.04). Additionally, there were no significant differences in pregnancy or neonatal birth outcomes between the two groups (all P > 0.05). These results suggest that IUI remains a viable and cost-effective option for infertile couples with UU infection who are facing infertility issues.
Humans ; Male ; Ureaplasma Infections/complications* ; Female ; Infertility, Male/therapy* ; Ureaplasma urealyticum/isolation & purification* ; Pregnancy ; Adult ; Pregnancy Outcome ; Semen Analysis ; Insemination, Artificial ; Semen/microbiology* ; China

OBJECTIVE: To explore the efficacy and apoptosis of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of acute myeloid leukemia (AML) with ASXL1 mutation. METHODS: The clinical data of 80 AML patients with ASXL1 mutation treated in our hospital from January 2019 to December 2021 were retrospectively analyzed. The clinical characteristics of the patients were summarized, and the therapeutic effect and prognostic factors of allo-HSCT for the patients were analyzed. RESULTS: Among the 80 patients, 38 were males and 42 were females, and the median age was 39(14-65) years. There were 17 patients in low-risk group, 25 patients in medium-risk group and 38 patients in high-risk group. ASXL1 mutation co-occurred with many other gene mutations, and the frequent mutated genes were TET2 (71.25%), NRAS (18.75%), DNMT3A (16.25%), NPM1 (15.00%), CEBPA (13.75%). Among medium and high-risk patients, 29 underwent allo-HSCT, while 34 received chemotherapy. The 2-year overall survival (OS) rate and disease-free survival (DFS) rate of the allo-HSCT group were 72.4% and 70.2%, while those of the chemotherapy group were 44.1% and 34.0%, respectively. The statistical analysis showed significant differences between the two groups (both P < 0.01). Multivariate analysis showed that age at transplantation >50- years and occurrence of acute graft-versus-host disease after transplantation were poor prognostic factors for OS and DFS in transplantation patients. CONCLUSION Allo-HSCT can improve the prognosis of AML patients with ASXL1 mutation.
Humans ; Leukemia, Myeloid, Acute/therapy* ; Hematopoietic Stem Cell Transplantation ; Female ; Male ; Middle Aged ; Mutation ; Adult ; Repressor Proteins/genetics* ; Adolescent ; Retrospective Studies ; Aged ; Nucleophosmin ; Young Adult ; Transplantation, Homologous ; Prognosis ; Survival Rate

Peri-implant keratinized mucosa (PIKM) augmentation refers to surgical procedures aimed at increasing the width of PIKM. Consensus reports emphasize the necessity of maintaining a minimum width of PIKM to ensure long-term peri-implant health. Currently, several surgical techniques have been validated for their effectiveness in increasing PIKM. However, the selection and application of PIKM augmentation methods may present challenges for dental practitioners due to heterogeneity in surgical techniques, variations in clinical scenarios, and anatomical differences. Therefore, clear guidelines and considerations for PIKM augmentation are needed. This expert consensus focuses on the commonly employed surgical techniques for PIKM augmentation and the factors influencing their selection at second-stage surgery. It aims to establish a standardized framework for assessing, planning, and executing PIKM augmentation procedures, with the goal of offering evidence-based guidance to enhance the predictability and success of PIKM augmentation.
Humans ; Consensus ; Dental Implants ; Mouth Mucosa/surgery* ; Keratins

AIM To optimize the extraction process for total polyphenols from Conioselinum vaginatu(Spreng.)Thell.,make component analysis,and evaluate their anti-oxidant,hypoglycemic activities.METHODS The effects of ultrasound,enzymatic hydrolysis,acid hydrolysis,alcohol extraction and hydrolysis processes on the extraction quantity of total polyphenols were investigated,respectively.With extraction temperature,extraction time,ethanol concetration and liquid-solid ratio as influencing factors,extraction quantity of total polyphenols as an evaluation index,the extraction process was optimized by response surface method.HPLC was adopted in the identification of polyphenolic composition and determination of their contents.Subsequently,total polyphenols' scavenging capacities on DPPH,ABTS,OH free radicals,total reducing power and inhibitory capacity on α-glucosidase were determined.RESULTS The highest extraction quantity of total polyphenols was observable when extraction process was employed.The optimal conditions were determined to be 62 ℃ for extraction temperature,54 min for extraction time,69％for ethanol concentration,and 50∶1 for liquid-solid ratio,the extraction quantity of total polyphenols was(9.51±0.2)mg GAE/g.Seven constituents existed in C.vaginatu,among which ferulic acid demonstrated the highest content,followed by that of myricetin,while D-tryptophan content was the lowest.At the concentration of 7.61 mg/L,total polyphenols displayed the scavenging rates on DPPH,ABTS,OH free radicals of 80.70％,85.97％,28.60％,total reducing power of 0.22,and inhibition rate on α-glucosidase of 77.23％,respectively.CONCLUSION This stable and reliable method can be used for the extraction of total polyphenols from C.vaginatum with strong anti-oxidant,hypoglycemic activities.

Obesity, as a chronic recurrent disease, has become a major public health challenge in China. To implement the requirements of the Healthy China Initiative (2019—2030), under domestic guidelines or consensus statements on overweight and obesity, and in alignment with the latest scientific advances globally, the Quality control protocol for adult overweight and obesity screening in health management (examination) institutions (2025 edition) was developed. This protocol was drafted by the Health Management Center of Shanghai Changzheng Hospital and formulated through multiple rounds of deliberation by experts in China’s health examination quality control field. The protocol establishes unified standards for screening facilities, personnel qualifications, and measurement or testing procedures. It defines specific screening items, outlines a standardized screening pathway, and sets requirements for the final medical review, ensuring the scientific validity, effectiveness, and safety of the screening process. The implementation of this protocol will enhance the consistency of weight management practices for adults across health examination institutions and strengthen the quality control of overweight and obesity screening programs.

AIM To optimize the extraction process for total polyphenols from Conioselinum vaginatu(Spreng.)Thell.,make component analysis,and evaluate their anti-oxidant,hypoglycemic activities.METHODS The effects of ultrasound,enzymatic hydrolysis,acid hydrolysis,alcohol extraction and hydrolysis processes on the extraction quantity of total polyphenols were investigated,respectively.With extraction temperature,extraction time,ethanol concetration and liquid-solid ratio as influencing factors,extraction quantity of total polyphenols as an evaluation index,the extraction process was optimized by response surface method.HPLC was adopted in the identification of polyphenolic composition and determination of their contents.Subsequently,total polyphenols' scavenging capacities on DPPH,ABTS,OH free radicals,total reducing power and inhibitory capacity on α-glucosidase were determined.RESULTS The highest extraction quantity of total polyphenols was observable when extraction process was employed.The optimal conditions were determined to be 62 ℃ for extraction temperature,54 min for extraction time,69％for ethanol concentration,and 50∶1 for liquid-solid ratio,the extraction quantity of total polyphenols was(9.51±0.2)mg GAE/g.Seven constituents existed in C.vaginatu,among which ferulic acid demonstrated the highest content,followed by that of myricetin,while D-tryptophan content was the lowest.At the concentration of 7.61 mg/L,total polyphenols displayed the scavenging rates on DPPH,ABTS,OH free radicals of 80.70％,85.97％,28.60％,total reducing power of 0.22,and inhibition rate on α-glucosidase of 77.23％,respectively.CONCLUSION This stable and reliable method can be used for the extraction of total polyphenols from C.vaginatum with strong anti-oxidant,hypoglycemic activities.

Objective To investigate the incidence rate,clinical characteristics,and prognosis of neonatal stroke in Shenzhen,China.Methods Led by Shenzhen Children's Hospital,the Shenzhen Neonatal Data Collaboration Network organized 21 institutions to collect 36 cases of neonatal stroke from January 2020 to December 2022.The incidence,clinical characteristics,treatment,and prognosis of neonatal stroke in Shenzhen were analyzed.Results The incidence rate of neonatal stroke in 21 hospitals from 2020 to 2022 was 1/15 137,1/6 060,and 1/7 704,respectively.Ischemic stroke accounted for 75％(27/36);boys accounted for 64％(23/36).Among the 36 neonates,31(86％)had disease onset within 3 days after birth,and 19(53％)had convulsion as the initial presentation.Cerebral MRI showed that 22 neonates(61％)had left cerebral infarction and 13(36％)had basal ganglia infarction.Magnetic resonance angiography was performed for 12 neonates,among whom 9(75％)had involvement of the middle cerebral artery.Electroencephalography was performed for 29 neonates,with sharp waves in 21 neonates(72％)and seizures in 10 neonates(34％).Symptomatic/supportive treatment varied across different hospitals.Neonatal Behavioral Neurological Assessment was performed for 12 neonates(33％,12/36),with a mean score of(32±4)points.The prognosis of 27 neonates was followed up to around 12 months of age,with 44％(12/27)of the neonates having a good prognosis.Conclusions Ischemic stroke is the main type of neonatal stroke,often with convulsions as the initial presentation,involvement of the middle cerebral artery,sharp waves on electroencephalography,and a relatively low neurodevelopment score.Symptomatic/supportive treatment is the main treatment method,and some neonates tend to have a poor prognosis.

Objective To evaluate the effect of potent lipid-lowering on serum proprotein convertase subtilisin/Kexin type 9(PCSK9)levels in patients undergoing percutaneous coronary intervention(PCI).Methods From January 2020 to June 2023,200 consecutive patients with low-density lipoprotein cholesterol(LDL-C)not reaching the required level(≥1.8 mmol/1)after PCI were enrolled in the Affiliated Hospital of Hangzhou Normal University.And all patients had taken 20 mg atorvastatin for more than 4 weeks before enrollment.They were randomly divided into intensive group(atorvastatin 40 mg/d)and combination group(atorvastatin 20 mg/d combined with ezetimibe 10 mg/d).At baseline,4 weeks and 12 weeks,the relevant blood lipid indexes were measured and compared.Including PCSK9,lipoprotein(a)[Lp(a)],total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),LDL-C,non high-density lipoprotein cholesterol(non-HDL-C).At the same time,we need to monitor liver function indicators,kidney function indicators,muscle enzyme indicators,etc.Results The TC,LDL-C and non-HDL-C of the two groups were decreased gradually compared with the baseline level at the follow-up of 4 and 12 weeks after the adjustment of the scheme(all P＜0.05).And the target rate of LDL-C in the combined group was higher(＜1.8 mmol/L:4 weeks P=0.001;12 weeks P＜0.001;＜1.4 mmol/L:4 weeks P=0.023,12 weeks P=0.001).In the intensive group,the PCSK9 level at 4 and 12 weeks of treatment was significantly higher than that before treatment,with statistically significant difference(4 weeks P＜0.001,12 weeks P=0.009).And with the extension of treatment time,the PCSK9 level at 12 weeks of treatment was significantly lower than that at 4 weeks of treatment(P＜0.001).The PCSK9 levels at 4 and 12 weeks after treatment in the combination group were not statistically significant compared with those before treatment(4 weeks P=0.292,12 weeks P=0.911).There was no correlation between PCSK9 at baseline and the change amplitude of LDL-C at 4 and 12 weeks of treatment in the intensive group and the combination group(The intensine group 4 weeks P=0.847,12 weeks P=0.450;The combination group 4 weeks P=0.053,12 weeks P=0.162).There was no correlation between PCSK9 change percentage and LDL-C change amplitude in the intensive group and the combination group at 4 and 12 weeks of treatment(The intensine group 4 weeks P=0.509,12 weeks P=0.085;The combination group 4 weeks P=0.475,12 weeks P=0.576).Before and after adjusting the medication plan in the intensive group,Lp(a)at 4 weeks of treatment was significantly higher than that before treatment,and the difference was statistically significant(P＜0.001).The Lp(a)levels at 4 and 12 weeks of treatment in the combination group were not statistically significant compared with those before treatment(4 weeks P=0.197,12 weeks P=0.058).Conclusions For patients with potent lipid-lowering after PCI,combined ezetimibe has a greater reduction in LDL-C and a higher Achievement rate than intensive statin therapy.Intensive statin therapy can increase the levels of PCSK9 and Lp(a)in a short time compared with combined ezetimibe therapy,suggesting that PCSK9 inhibitors should also be applied early and continuously.

Objective To evaluate the effect of potent lipid-lowering on serum proprotein convertase subtilisin/Kexin type 9(PCSK9)levels in patients undergoing percutaneous coronary intervention(PCI).Methods From January 2020 to June 2023,200 consecutive patients with low-density lipoprotein cholesterol(LDL-C)not reaching the required level(≥1.8 mmol/1)after PCI were enrolled in the Affiliated Hospital of Hangzhou Normal University.And all patients had taken 20 mg atorvastatin for more than 4 weeks before enrollment.They were randomly divided into intensive group(atorvastatin 40 mg/d)and combination group(atorvastatin 20 mg/d combined with ezetimibe 10 mg/d).At baseline,4 weeks and 12 weeks,the relevant blood lipid indexes were measured and compared.Including PCSK9,lipoprotein(a)[Lp(a)],total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),LDL-C,non high-density lipoprotein cholesterol(non-HDL-C).At the same time,we need to monitor liver function indicators,kidney function indicators,muscle enzyme indicators,etc.Results The TC,LDL-C and non-HDL-C of the two groups were decreased gradually compared with the baseline level at the follow-up of 4 and 12 weeks after the adjustment of the scheme(all P＜0.05).And the target rate of LDL-C in the combined group was higher(＜1.8 mmol/L:4 weeks P=0.001;12 weeks P＜0.001;＜1.4 mmol/L:4 weeks P=0.023,12 weeks P=0.001).In the intensive group,the PCSK9 level at 4 and 12 weeks of treatment was significantly higher than that before treatment,with statistically significant difference(4 weeks P＜0.001,12 weeks P=0.009).And with the extension of treatment time,the PCSK9 level at 12 weeks of treatment was significantly lower than that at 4 weeks of treatment(P＜0.001).The PCSK9 levels at 4 and 12 weeks after treatment in the combination group were not statistically significant compared with those before treatment(4 weeks P=0.292,12 weeks P=0.911).There was no correlation between PCSK9 at baseline and the change amplitude of LDL-C at 4 and 12 weeks of treatment in the intensive group and the combination group(The intensine group 4 weeks P=0.847,12 weeks P=0.450;The combination group 4 weeks P=0.053,12 weeks P=0.162).There was no correlation between PCSK9 change percentage and LDL-C change amplitude in the intensive group and the combination group at 4 and 12 weeks of treatment(The intensine group 4 weeks P=0.509,12 weeks P=0.085;The combination group 4 weeks P=0.475,12 weeks P=0.576).Before and after adjusting the medication plan in the intensive group,Lp(a)at 4 weeks of treatment was significantly higher than that before treatment,and the difference was statistically significant(P＜0.001).The Lp(a)levels at 4 and 12 weeks of treatment in the combination group were not statistically significant compared with those before treatment(4 weeks P=0.197,12 weeks P=0.058).Conclusions For patients with potent lipid-lowering after PCI,combined ezetimibe has a greater reduction in LDL-C and a higher Achievement rate than intensive statin therapy.Intensive statin therapy can increase the levels of PCSK9 and Lp(a)in a short time compared with combined ezetimibe therapy,suggesting that PCSK9 inhibitors should also be applied early and continuously.