Objective To investigate the application value of ankle-brachial index(ABI)combined with Wagner scale in the prognosis assessment in patients with diabetic foot(DF).Methods A total of 120 patients with DF were enrolled in this study from in our hospital from June 2020 to December 2021.All the patients were divided into good prognosis group(n=92)and poor prognosis group(n=28)according to the prognosis after 6 months.The ABI and related clinical indicators were compared between the two groups.Logistic regression analysis and receiver operating characteristic(ROC)curve were used to analyze the prognostic value of each index in patients with DF.Results The DF duration,HbA1c,white blood cell count,Wagner grade 3 to 5,antibiotic type≥3,defibration times≥3 were higher in the poor prognosis group than in the good prognosis group(P<0.05),while the albumin,TG,ABI,hemoglobin and revasectomy rates were lower in the poor prognosis group than in the good prognosis group(P<0.05).Logistic regression analysis showed that HbA1c(OR 3.232,95%CI 1.063～9.830),ABI(OR 0.150,95%CI 0.038～0.588),Wagner grading(OR 5.032,95%CI 1.258～20.128)were the prognostic factor for patients with DF.The driving equation was established:Logit(P)=1.908×Wagner grading-1.978×ABI-1.945.ROC curve analysis showed that the area under the curve(0.897)of driving equation was higher than ABI(0.754)and Wagner grading(0.810)(P<0.05)in predicting poor prognosis in patients with DF.Conclusions ABI and Wagner grading are both factors affecting the poor prognosis of patients with DF,and ABI combined with Wagner grading is more effective in predicting the prognosis of patients with DF.

Objective To investigate the application value of ankle-brachial index(ABI)combined with Wagner scale in the prognosis assessment in patients with diabetic foot(DF).Methods A total of 120 patients with DF were enrolled in this study from in our hospital from June 2020 to December 2021.All the patients were divided into good prognosis group(n=92)and poor prognosis group(n=28)according to the prognosis after 6 months.The ABI and related clinical indicators were compared between the two groups.Logistic regression analysis and receiver operating characteristic(ROC)curve were used to analyze the prognostic value of each index in patients with DF.Results The DF duration,HbA1c,white blood cell count,Wagner grade 3 to 5,antibiotic type≥3,defibration times≥3 were higher in the poor prognosis group than in the good prognosis group(P<0.05),while the albumin,TG,ABI,hemoglobin and revasectomy rates were lower in the poor prognosis group than in the good prognosis group(P<0.05).Logistic regression analysis showed that HbA1c(OR 3.232,95%CI 1.063～9.830),ABI(OR 0.150,95%CI 0.038～0.588),Wagner grading(OR 5.032,95%CI 1.258～20.128)were the prognostic factor for patients with DF.The driving equation was established:Logit(P)=1.908×Wagner grading-1.978×ABI-1.945.ROC curve analysis showed that the area under the curve(0.897)of driving equation was higher than ABI(0.754)and Wagner grading(0.810)(P<0.05)in predicting poor prognosis in patients with DF.Conclusions ABI and Wagner grading are both factors affecting the poor prognosis of patients with DF,and ABI combined with Wagner grading is more effective in predicting the prognosis of patients with DF.

Mutations in genes encoding amyloid precursor protein (APP) and presenilins (PSs) cause familial forms of Alzheimer's disease (AD), a neurodegenerative disorder strongly associated with aging. It is currently unknown whether and how AD risks affect early brain development, and to what extent subtle synaptic pathology may occur prior to overt hallmark AD pathology. Transgenic mutant APP/PS1 over-expression mouse lines are key tools for studying the molecular mechanisms of AD pathogenesis. Among these lines, the 5XFAD mice rapidly develop key features of AD pathology and have proven utility in studying amyloid plaque formation and amyloid β (Aβ)-induced neurodegeneration. We reasoned that transgenic mutant APP/PS1 over-expression in 5XFAD mice may lead to neurodevelopmental defects in early cortical neurons, and performed detailed synaptic physiological characterization of layer 5 (L5) neurons from the prefrontal cortex (PFC) of 5XFAD and wild-type littermate controls. L5 PFC neurons from 5XFAD mice show early APP/Aβ immunolabeling. Whole-cell patch-clamp recording at an early post-weaning age (P22-30) revealed functional impairments; although 5XFAD PFC-L5 neurons exhibited similar membrane properties, they were intrinsically less excitable. In addition, these neurons received smaller amplitude and frequency of miniature excitatory synaptic inputs. These functional disturbances were further corroborated by decreased dendritic spine density and spine head volumes that indicated impaired synapse maturation. Slice biotinylation followed by Western blot analysis of PFC-L5 tissue revealed that 5XFAD mice showed reduced synaptic AMPA receptor subunit GluA1 and decreased synaptic NMDA receptor subunit GluN2A. Consistent with this, patch-clamp recording of the evoked L23>L5 synaptic responses revealed a reduced AMPA/NMDA receptor current ratio, and an increased level of AMPAR-lacking silent synapses. These results suggest that transgenic mutant forms of APP/PS1 overexpression in 5XFAD mice leads to early developmental defects of cortical circuits, which could contribute to the age-dependent synaptic pathology and neurodegeneration later in life.
Mice ; Animals ; Alzheimer Disease/pathology* ; Amyloid beta-Peptides/metabolism* ; Receptors, N-Methyl-D-Aspartate/metabolism* ; Amyloid beta-Protein Precursor/metabolism* ; Mice, Transgenic ; Neurons/metabolism* ; Receptors, AMPA/metabolism* ; Disease Models, Animal

BACKGROUND:Bone marrow mesenchymal stem cells(MSCs)are a kind of adult stem cells with multi-potential differentiation property.At present,it has served as cell carrier for the treatment of Parkinson's disease.OBJECTIVE:To construct pDsRed-C1-CDNF eukaryotic expression vector and induce its expression in rat MSCs.METHODS:CDNF gene was amplified from mouse tissues using RT-PCR,and sequence with Xho I,BamHI restriction enzyme cutting site.The CDNF gene was inserted into the eukaryotic expression vector pDsRed-C1 encoding red fluorescent protein gene.The plasmid pDsRed-C1-CDNF was constructed and transfected into rat bone marrow MSCs.RESULES AND CONCLUSION:The pDsRed-C1-CDNF recombinant plasmid was confirmed by double digestion of Xho I and BamHI restriction enzyme or single digestion of BamHI,and PCR sequence.Results show that the recombinant pDsRed-C1-CDNF eukaryotic expression vector was successfully constructed.