Objective To assess the current status of occupational radiation hazards in animal diagnosis and treatment institutions in Foshan City. Methods A total of 214 animal diagnosis and treatment institutions in Foshan City in 2024 were selected as the study subjects using the judgment sampling method. The radiation protection management status was investigated. Results A total of 178 out of the 214 animal diagnosis and treatment institutions were equipped with radiation diagnostic equipment in Foshan City. Among these 178 institutions, 98 (accounting for 55.1%) obtained permits from the ecology and environmental department, 21 (accounting for 11.8%) completed occupational hazard project declarations, 53 (accounting for 29.8%) conducted workplace radiation level monitoring, 132 (accounting for 74.2%) were equipped with radiation protection equipment, 40 (accounting for 22.5%) conducted occupational health examinations for the radiation staff, 39 (accounting for 21.9%) provided radiation protection knowledge training for the radiation staff, and 52 (accounting for 29.2%) performed personal radiation dose monitoring. However, none of the institutions implemented the “Three Simultaneities (design, construct, put into operation and use simultaneously with the main body of the construction project)” system for occupational disease prevention facilities in construction projects. Conclusion sAnimal diagnostic and treatment institutions in Foshan City have low levels of radiation protection management and inadequate occupational health monitoring. The radiation staff has low awareness of radiation protection, Relevant department should strengthen supervision and management, organize radiation protection knowledge training, and standardize occupational health management to effectively safeguard workers' health rights.

PURPOSE: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secretion in mouse Leydig cells. MATERIALS AND METHODS: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine monophosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were analyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. RESULTS: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secretion in mouse Leydig cells. CONCLUSIONS: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduction, which may help us to treat male infertility.
Animals ; Apoptosis ; Blotting, Western ; Cell Count ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Proliferation ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Germ Cells ; Guanosine Monophosphate ; Humans ; Infertility, Male ; Lentivirus ; Leydig Cells ; Male ; Mice ; Natriuretic Peptide, C-Type ; Polymerase Chain Reaction ; Receptors, Peptide ; Reproduction ; Reverse Transcription ; RNA Interference ; S Phase ; Testicular Diseases ; Testis ; Testosterone

Objective: To analyze the clinical application of the open supraclavicular approach in thyroidectomy. Methods: The clinical practicability of open supraclavicular thyroidectomy was explored by comparing the traditional anterior low arc incision thyroidectomy procedure with open supraclavicular thyroidectomy in terms of patients'aesthetic satisfaction, effectiveness of the operation, operation time, and so on. Result: Twenty-two cases of open supraclavicular thyroidectomy (group B) had better aesthetic satisfaction than 29 cases of traditional incision thyroidectomy (group A)(P<0.05), and had the same operative effect with traditional incision. Open supraclavicular thyroidectomy is associated with good aesthetic satisfaction, and has the same effect as the traditional incision does. Conclusions: Open supraclavicular thyroidectomy has good clinical value for benign thyroid tumors and some malignant tumors that require unilateral lobectomy, and even for tumors larger than the incision diameter. It has good aesthetic value while ensuring the curative effect of surgery.

Objective To observe the effects of adenosine preconditioning on the intestinal inflammatory response,the damaged mucosal tissue and apoptosis of intestinal epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A had 0.9％ sodium chloride solution immersion + caudal vein injection of sterilization water (3 mg/kg).The animals in group B received seawater immersion + caudal vein injection of sterile water (3 mg/kg) and the animals in group C were given seawater immersion + caudal vein injection of adenosine pretreatment (3 mg/kg).All animals were sacrificed 6 hours after immersion.Pathological changes in intestinal mucosa were closely observed by histopathology.Levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA).Caspase-3 protein was detected by immunohistochemistry.Apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The intestinal pathological scores of TNF-α and IL-6 for the animals in group B(3.98 ±0.41)were significantly higher than those in group A (2.46 ±0.39) (P ＜0.01).The TNF-α and IL-6 levels for the animals in group B [(2.38 ±0.39) μg/L and(883.11 ±34.22) ng/L] were significantly increased,as compared with those in group A [(1.01 ± 0.06) μg/L and (258.09 ± 6.52) ng/L] (P ＜ 0.01).The TNF-α and IL-6 levels for the animals in group C[(1.88 ± 0.21)pg/L and (582.13 ±19.44)ng/L] were significantly decreased,as compared with those in group B (P ＜ 0.01).The level of Caspase-3 and percentage of apoptotic cells in the intestinal mucosa of the animals in group B [(27.9 ± 4.3) ％ and (24.5 ± 3.1) ％]were significantly higher than those of the animals in group A [(13.8 ± 2.6) ％ and (16.4 ± 2.3) ％] (P ＜0.01),and the expression level of Caspase-3 and percentage of apoptotic cells of the animals in group C [(20.2 ± 3.4) ％ and (19.9 ± 2.9) ％] were significantly lower than those of the animals in group B (P ＜ 0.01).Conclusions Adenosine pretreatment could significantly improve intestinal mucosal inflammatory response in seawater immersion rats coupled with open abdominal open injury,alleviate the destruction of intestinal epithelial structure,and reduce apoptosis of intestinal mucosal epithelial cells.

Objective To observe the effects of adenosine preconditioning on gastric mucosal tissue destruction,inflammatory response and apoptosis of epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A were treated with abdominal open injury + caudal vein injection of sterile water (3 mg/kg).The animals with abdominal open injury in group B received seawater immersion + caudal vein injection of sterile water (3 mg/kg),and the animals with abdominal open injury in group C were given seawater immersion + caudal vein injection of adenosine (3 mg/kg).All the animals were treated either with caudal vein injection of sterile water or adenosine at a dosage of 3 mg/kg 60 minutes before surgery,and were sacrificed 6 hours after immersion.Gastric mucosal ulcer index (UI) was calculated in the animals of all the groups.Pathological changes in gastric mucosa were closely observed under light microscopy.The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in the gastric mucosa of various groups were detected by enzyme-linked immunosorbent assay (ELISA),the expression levels of Caspase-3 in gastric mucosa were detected by immunohistochemistry,and apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The UI,the levels of TNF-α and IL-6,and the pathological damage of gastric mucosa in group B were all significantly higher than those in group A (P＜0.01) and group C (P ＜0.05).The expression level of Caspase-3 (25.2％ ±3.6％) and the percentage of apoptosis cells (27.3％ ± 2.4％) in group B were significantly higher than those in group A (10.3％ ±1.4％ and 9.4％ ± 1.8％) (P ＜0.01),and the expression level of Caspase-3 of group C (19.4％ ±2.6％ and 21.9％ ± 2.6％) were signficantly lower than those of group B (P ＜0.05).Conclusions Adenosine pretreatment could effectively protect gastric mucosal epithelial inflammatory response in seawater immersion rats coupled with open abdominal injury,inhibit the secretion of inflammatory factors,alleviate gastric mucosal inflammatory response and reduce apoptosis of gastric mucosal epithelial cells.

Objective To observe the effects of adenosine preconditioning on the intestinal inflammatory response,the damaged mucosal tissue and apoptosis of intestinal epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A had 0.9％ sodium chloride solution immersion + caudal vein injection of sterilization water (3 mg/kg).The animals in group B received seawater immersion + caudal vein injection of sterile water (3 mg/kg) and the animals in group C were given seawater immersion + caudal vein injection of adenosine pretreatment (3 mg/kg).All animals were sacrificed 6 hours after immersion.Pathological changes in intestinal mucosa were closely observed by histopathology.Levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA).Caspase-3 protein was detected by immunohistochemistry.Apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The intestinal pathological scores of TNF-α and IL-6 for the animals in group B(3.98 ±0.41)were significantly higher than those in group A (2.46 ±0.39) (P ＜0.01).The TNF-α and IL-6 levels for the animals in group B [(2.38 ±0.39) μg/L and(883.11 ±34.22) ng/L] were significantly increased,as compared with those in group A [(1.01 ± 0.06) μg/L and (258.09 ± 6.52) ng/L] (P ＜ 0.01).The TNF-α and IL-6 levels for the animals in group C[(1.88 ± 0.21)pg/L and (582.13 ±19.44)ng/L] were significantly decreased,as compared with those in group B (P ＜ 0.01).The level of Caspase-3 and percentage of apoptotic cells in the intestinal mucosa of the animals in group B [(27.9 ± 4.3) ％ and (24.5 ± 3.1) ％]were significantly higher than those of the animals in group A [(13.8 ± 2.6) ％ and (16.4 ± 2.3) ％] (P ＜0.01),and the expression level of Caspase-3 and percentage of apoptotic cells of the animals in group C [(20.2 ± 3.4) ％ and (19.9 ± 2.9) ％] were significantly lower than those of the animals in group B (P ＜ 0.01).Conclusions Adenosine pretreatment could significantly improve intestinal mucosal inflammatory response in seawater immersion rats coupled with open abdominal open injury,alleviate the destruction of intestinal epithelial structure,and reduce apoptosis of intestinal mucosal epithelial cells.

Objective To observe the effects of adenosine preconditioning on gastric mucosal tissue destruction,inflammatory response and apoptosis of epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A were treated with abdominal open injury + caudal vein injection of sterile water (3 mg/kg).The animals with abdominal open injury in group B received seawater immersion + caudal vein injection of sterile water (3 mg/kg),and the animals with abdominal open injury in group C were given seawater immersion + caudal vein injection of adenosine (3 mg/kg).All the animals were treated either with caudal vein injection of sterile water or adenosine at a dosage of 3 mg/kg 60 minutes before surgery,and were sacrificed 6 hours after immersion.Gastric mucosal ulcer index (UI) was calculated in the animals of all the groups.Pathological changes in gastric mucosa were closely observed under light microscopy.The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in the gastric mucosa of various groups were detected by enzyme-linked immunosorbent assay (ELISA),the expression levels of Caspase-3 in gastric mucosa were detected by immunohistochemistry,and apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The UI,the levels of TNF-α and IL-6,and the pathological damage of gastric mucosa in group B were all significantly higher than those in group A (P＜0.01) and group C (P ＜0.05).The expression level of Caspase-3 (25.2％ ±3.6％) and the percentage of apoptosis cells (27.3％ ± 2.4％) in group B were significantly higher than those in group A (10.3％ ±1.4％ and 9.4％ ± 1.8％) (P ＜0.01),and the expression level of Caspase-3 of group C (19.4％ ±2.6％ and 21.9％ ± 2.6％) were signficantly lower than those of group B (P ＜0.05).Conclusions Adenosine pretreatment could effectively protect gastric mucosal epithelial inflammatory response in seawater immersion rats coupled with open abdominal injury,inhibit the secretion of inflammatory factors,alleviate gastric mucosal inflammatory response and reduce apoptosis of gastric mucosal epithelial cells.

Objective To investigate the effect of infiltrated mast cells on the biological characteristics of uterine carcinoma.Methods Twelve leiomyomas,ten uncertain malignant uterine leiomyomas,and seven uterine leiomyosarcomas were studied with light microscopy for the morphometry about their histological feature,mast cell particle and factor Ⅷ-related antigen(FⅧRAg);Cell apoptosis was measured by flow cytometry.The correlative analysis was carried out between the mast cell and others factors.Results Comparing with smooth muscle tumors of uncertain malignant potential(4209.9?273.0),the count of tumor cell was the highest in leiomyosarcoma(3557.6?346.3)(P