Objective To analyze the clinical characteristics of patients with Streptococcus anginosus group(SAG)infected brain abscess and provide a reference basis for the clinical diagnosis and treatment of this disease.Methods The clinical information of 59 patients with brain abscess with SAG in Guangdong Sanjiu Brain Hospital from January 2016 to October 2023 was collected for retrospective statistical analysis.Results Among the 59 patients with brain abscess,there were 52(88.14%)male and 7(11.86%)female.Among 59 cases of SAG,there were 32(54.24%)in the Str.intermedius group,21(35.59%)in the Str.constellatus group and 6(10.17%)in the Str.anginosus group.The age of Str.intermedius group was less than that of Streptococcus constellatus group(36.75±20.06 years vs 50.43±16.17 years),and the difference was statistically significant(t=2.614,P<0.05).The common clinical symptom was headache,while the probability of fever was greater in the Str.intermedius group compared to the Str.constellatus group(t=4.174,P<0.05).Abscesses were commonly solitary and were located in the parietal and frontal lobes of the brain.Patients with Str.constellatus had a greater probability of developing mixed infections compared to patiens with Str.intermedius(57.14%vs 9.38%)with significant differences(t=14.257,P<0.05).Glycopeptide(vancomycin)plus carbapenem(meropenem)combination regimen was the most commonly used in both empiric and targeted dosing were 64.41%and 52.54%,respectively.No strains resistant to common antibiotics were identified.Conclusion The SAG causing brain abscesses are mainly Str.intermedius and Str.constellatus,and the age,symptoms and infection patterns of patients with brain abscesses caused by the two bacteria were different.SAG did not appear to be drug-resistant in the clinic,and the clinic could reasonably evaluate and adjust the anti-infection regimen based on the drug sensitivity results.

Objective To analyze the clinical characteristics of patients with Streptococcus anginosus group(SAG)infected brain abscess and provide a reference basis for the clinical diagnosis and treatment of this disease.Methods The clinical information of 59 patients with brain abscess with SAG in Guangdong Sanjiu Brain Hospital from January 2016 to October 2023 was collected for retrospective statistical analysis.Results Among the 59 patients with brain abscess,there were 52(88.14%)male and 7(11.86%)female.Among 59 cases of SAG,there were 32(54.24%)in the Str.intermedius group,21(35.59%)in the Str.constellatus group and 6(10.17%)in the Str.anginosus group.The age of Str.intermedius group was less than that of Streptococcus constellatus group(36.75±20.06 years vs 50.43±16.17 years),and the difference was statistically significant(t=2.614,P<0.05).The common clinical symptom was headache,while the probability of fever was greater in the Str.intermedius group compared to the Str.constellatus group(t=4.174,P<0.05).Abscesses were commonly solitary and were located in the parietal and frontal lobes of the brain.Patients with Str.constellatus had a greater probability of developing mixed infections compared to patiens with Str.intermedius(57.14%vs 9.38%)with significant differences(t=14.257,P<0.05).Glycopeptide(vancomycin)plus carbapenem(meropenem)combination regimen was the most commonly used in both empiric and targeted dosing were 64.41%and 52.54%,respectively.No strains resistant to common antibiotics were identified.Conclusion The SAG causing brain abscesses are mainly Str.intermedius and Str.constellatus,and the age,symptoms and infection patterns of patients with brain abscesses caused by the two bacteria were different.SAG did not appear to be drug-resistant in the clinic,and the clinic could reasonably evaluate and adjust the anti-infection regimen based on the drug sensitivity results.

Objective To investigate the effect of ultrasound-guided erector spinae plane block(ESPB)on early postoperative respiratory function and inflammatory cytokines in patients with multiple rib fractures(MRFs).Methods Fifty-eight patients who underwent MRFs surgery,42 males and 16 females,aged 18-64 years,BMI 18.5-30.0 kg/m2,ASA physical status Ⅰ or Ⅱ,were selected from February 2019 to December 2021.The patients were divided into two groups using random number method:ESPB combined with general anesthesia group(group E)and general anesthesia alone group(group G),29 pa-tients in each group.All the patients in group E underwent ultrasound-guided ESPB in the lateral decubitus position after general anesthesia induction,and 0.5％ropivacaine 0.4 ml/kg was administered.Forced vital capacity(FVC),arterial blood gas analysis,VAS pain scores at rest and cough were recorded before anes-thesia induction,at discharge from PACU,24 and 48 hours after operation.The number of effective PCIA compressions during 0-24 hours and 24-48 hours after surgery and the number of rescue analgesia were re-corded.The concentrations of IL-6 and TNF-α were recorded before anesthesia induction,24 and 48 hours after operation.Results Compared with group G,the FVC was significantly higher,and the VAS score and PaCO2 were significantly lower in group E at discharge from PACU,24 and 48 hours after operation(P＜0.05).The number of effective PCIA compressions during 0-24 hours and 24-48 hours after surgery,the rate of rescue analgesia,the concentrations of IL-6 and TNF-α 24 and 48 hours after operation in group E were significantly lower than those in group G(P＜0.05).Conclusion Ultrasound-guided ESPB can pro-vide good postoperative analgesia,promote early postoperative recovery of respiratory function in patients with MRFs.

Livestock husbandry is vital to economy of the Tarim Basin, Xinjiang Autonomous Region, China. However, there have been few surveys of the distribution of ixodid ticks (Acari: Ixodidae) and tick-borne pathogens affecting domestic animals at these locations. In this study, 3,916 adult ixodid ticks infesting domestic animals were collected from 23 sampling sites during 2012-2016. Ticks were identified to species based on morphology, and the identification was confirmed based on mitochondrial 16S and 12S rRNA sequences. Ten tick species belonging to 4 genera were identified, including Rhipicephalus turanicus, Hyalomma anatolicum, Rh. bursa, H. asiaticum asiaticum, and Rh. sanguineus. DNA sequences of Rickettsia spp. (spotted fever group) and Anaplasma spp. were detected in these ticks. Phylogenetic analyses revealed possible existence of undescribed Babesia spp. and Borrelia spp. This study illustrates potential threat to domestic animals and humans from tick-borne pathogens.

[ ABSTRACT] AIM:To investigate the depressant effect of FK228 combined with rapamycin on the human breast cancer cell line MCF-7 and MDA-MB-435.METHODS:FK228, a new histone deacetylase inhibitor, and rapamycin, the specific inhibitor of the mammalian target of rapamycin ( mTOR) protein, were used in the study.MCF-7 cells and MDA-MB-435 cells were exposed to different concentrations of FK228 and rapamycin.The inhibitory rate of cell growth was de-termined by SRB assay.Combination index ( CI) was used to evaluate the interaction between FK228 and rapamycin.The expression of the apoptotic proteins, cycle proteins and nucleic acid proteins were detected by Western blotting.The cell cycle was analyzed by flow cytometry.RESULTS: Both FK228 and rapamycin showed growth inhibitory effects on the breast cancer cell lines in a time-and dose-dependent manner.CI of the 2 drugs was less than 1 when the inhibitory rate of the cell growth was 50%effective dose (ED50)~ED70, indicating a synergistic effect.The combination therapy of FK228 with rapamycin increased the apoptotic proteins, and induced the down-regulation of phosphorylated Akt and over-expres-sion of caspase-3 compared with a single use of the drugs.The combination therapy of FK228 with rapamycin reduced the cycle proteins, and the cell cycle was arrested in G2/M.The levels of phosphorylated H2AX and acetylated H3 were ob-viously increased after combination therapy.CONCLUSION:The combination therapy of FK228 with rapamycin inhibits the cell proliferation and increases apoptosis with a synergistic effect, which may become a new trend for treating endometri-al cancer.

Objective To discuss the value of multi-slice spiral CT angiography (MSCTA)and digital subtraction angiography (DSA)in lower extremity arteriosclerosis obliterans.Methods MSCTA and DSA of lower extremity arteries were performed in 41 patients with lower extremity arteriosclerosis obliterans sequentially.The interval of these two examinations was no more than 2 weeks.DSA served as the reference standard and compared with MSCTA in terms of grading of stenoocclusive lesions,visualization of collaterals in lower extremity arteries.Results No significant differences of grading of stenoocclusive lesions,visualization of col-laterals were observed between MCTA and DSA in lower extremity arteries.Compared with the results of DSA,the sensitivity,spe-cificity,accuracy,positive and negative predictive value of MSCTA in the detection of arterial stenosis (≥ 50%)were 97.2%, 98.2%,93.8%,94.6%,98.2%,respectively.Conclusion MSCTA shows relatively high sensitivity and specificity in the diagnosis of lower extremity arteriosclerosis obliterans,can provide effective guidance of planning treatment in lower extremity arteriosclerosis obliterans.

BACKGROUND: Diabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear. OBJECTIVE: We investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model. METHODS: Forty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery. RESULTS: Wound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-beta1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls. CONCLUSION: The combination of BMSCs and PRP aids diabetic wound repair and regeneration.
Amputation ; Animals ; Bone Marrow ; Cell Adhesion ; Diabetes Mellitus ; Gangrene ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; Mesenchymal Stromal Cells* ; Models, Animal ; Platelet-Rich Plasma* ; Proliferating Cell Nuclear Antigen ; Rats* ; Regeneration ; Skin ; Streptozocin ; Ulcer ; Wound Healing ; Wounds and Injuries

Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.

Objective To study the Hainan Utstein templates used for cardiac arrest and resuscitation registries to evaluate the epidemiological characteristics and outcomes of the patients with CPR by multi-center study. Methodsccording to the Utstein templates for cardiac arrest and CPR set by International Liaison Committee on resuscitation in 2004, a Hainan Utstein CPR registry chart was designed and a prospective descriptive study was carried out to evaluate the epidemiological characteristics, impact factors and outcomes of the patients with resuscitation attempt in emergency departments of thirteen hospitals in Hainan Island between January 2007 and December 2010.Results Of 1125 patients with cardiac arrest, male accounted for 73. 8％ and female was 26. 2％. The mean ( ± S. D) age of the cardiac arrest patients was 53.9 ± 13. 1 years old.Coronary heart diseases and hypertension were the most common preexisting chronic diseases in the studied patients. The ROSC rate and discharge rates after survival in 1125 patients with CPR were 23. 8％ and 7.4％ respectively. The ROSC rate and discharge rates after survival were 36. 3％ and 11.6％ in the in-hospital cardiac arrest (IHCA) group, respectively whereas 11.5％ and 3. 3％ in out-hospital cardiac arrest (OHCA) group. Of 188 patients with ventricular fibrillation/Pulseless ventricular tachycardia, the ROSC rate and discharge rate after survival were 58.0％and 21.8％,respectively. Of them, 448 (39. 8％ ) of the cardiac arrest patients had underlying cardiac causes, and the ROSC rate and discharge rate after survival were 36. 3％ and 11.5％ respectively in IHCA group whereas 11.6％ and 3. 3％ in OHCA group. The ROSC rate and discharge rate after survival were 69. 8％ and 7. 4％respectively in the tertiary hospitals whereas 30. 2％ and 7. 3％ in the secondary hospitals. Conclusions Patients experienced cardiac arrest were predominantly male. Coronary heart disease and hypertension were the two most common preexisting chronic diseases. The ROSC rate and discharge rate of patients with IHCA were higher than those with OHCA. ROSC rate and discharge rate after survival were higher in the ventriculat fibrillation/Pulseless ventricular tachycardia group than the other cardiac rhythms first witnessed groups. The time delayed of starting CPR after onset of cardiac arrest had a critical impact on survival and discharge rate in both IHCA and OHCA groups.

Objective To study the risk factors of complications after hepatectomy for intrahepatic stone.Method Clinical data of 363 patients with hepatolithiasis receiving elected hepatic resection in past 4 years in our hospital were analyzed retrospectively.Postoperative complications were recorded.Possible risk factors pertaining to the development of complications were identified using univariate and multivariate analysis.Results the surgical mortality was 0.6%(2/363).Postoperative complications occurred in 110 of 363 patients(30.3%).The morbidity included wound infection/liquafication in 42 cases(11.6%),pleural effusion in 41(11.3%),subphrenic collection in 36 (9.9%),bile leakage in 21(5.8%),intra-abdominal collection/infection in 13(3.6%),gastrointestinal bleeding in 3(0.8%),biliary hemorrhage in 2(0.6%),hepatic function insufficiency in 1 (0.3%).The multivariate logistic regression analysis identified that previous history of biliary surgery (P=0.042,odds ratio[OR]=0.617,95%confidence interval[CI]:0.388 to 0.982)and operative time(P=0.0001,odds ratio[OR]=0.994,95%confidence interval[CI]:0.991 to 0.997)were the independent risk factors for development of complications after hepatoectomy for hepatolithiasis.Conclusion Previous history of biliary duct surgery and operative time are the independent risk factors for development of complications after hepatoectomy for intrahepatic stone.For patients with previous biliary surgery,adequate preoperative preparation,improving operative skills and shortening operative time are critical important to prevent occurrence of complications.