Objective To explore the relationship between triglyceride-glucose(TyG)index and hypertension in patients with cerebral hemorrhage.Methods A total of 1718 patients with cere-bral hemorrhage admitted to our hospital from January,2013 to May,2023 were enrolled in this study.According to the TyG index quartile,437 cases were assigned into Q1 group(≤8.375),424 cases into Q2 group(TyG index 8.376～8.737),429 cases into Q3 group(TyG index 8.738～9.087),and 428 cases into Q4 group(≥9.088).The general clinical data were compared in the four groups.Logistic regression analysis was used to study the correlation between TyG index and hy-pertension.Results There were significant differences among the four groups in terms of age,hy-pertension,diabetes,SBP,DBP,TC,TG,HDL-C,LDL-C,FBG,glycated hemoglobin and TyG in-dex(P＜0.05,P＜0.01).Logistic regression analysis showed that when the TyG index was a con-tinuous variable,it was significantly correlated with the risk of hypertension(OR=1.999,95％CI:1.393-2.869,P=0.001).When the index was used as a categorical variable,with Q1as a ref-erence,TyG index in Q3 and Q4 was associated with an increase in OR of hypertension(OR=1.869,95％CI:1.220-2.865,P=0.004;OR=1.844,95％CI:1.125-3.020,P=0.015).After ad-justing cofounders,the association of TyG index and risk of hypertension was stronger in the fe-males(OR=2.618,95％CI:1.312-5.221,P=0.006)than the males(OR=1.783,95％CI:1.151-2.761,P=0.010),and in the patients ≥65 years old(OR=3.277,95％CI:1.600-6.741,P=0.001)than those＜65 years old(OR=1.782,95％CI:1.076-2.949,P=0.025).Conclusion TyG index is closely associated with hypertension in patients with cerebral hemorrhage,especially in women and elderly.

Objective To explore the relationship between triglyceride-glucose(TyG)index and hypertension in patients with cerebral hemorrhage.Methods A total of 1718 patients with cere-bral hemorrhage admitted to our hospital from January,2013 to May,2023 were enrolled in this study.According to the TyG index quartile,437 cases were assigned into Q1 group(≤8.375),424 cases into Q2 group(TyG index 8.376～8.737),429 cases into Q3 group(TyG index 8.738～9.087),and 428 cases into Q4 group(≥9.088).The general clinical data were compared in the four groups.Logistic regression analysis was used to study the correlation between TyG index and hy-pertension.Results There were significant differences among the four groups in terms of age,hy-pertension,diabetes,SBP,DBP,TC,TG,HDL-C,LDL-C,FBG,glycated hemoglobin and TyG in-dex(P＜0.05,P＜0.01).Logistic regression analysis showed that when the TyG index was a con-tinuous variable,it was significantly correlated with the risk of hypertension(OR=1.999,95％CI:1.393-2.869,P=0.001).When the index was used as a categorical variable,with Q1as a ref-erence,TyG index in Q3 and Q4 was associated with an increase in OR of hypertension(OR=1.869,95％CI:1.220-2.865,P=0.004;OR=1.844,95％CI:1.125-3.020,P=0.015).After ad-justing cofounders,the association of TyG index and risk of hypertension was stronger in the fe-males(OR=2.618,95％CI:1.312-5.221,P=0.006)than the males(OR=1.783,95％CI:1.151-2.761,P=0.010),and in the patients ≥65 years old(OR=3.277,95％CI:1.600-6.741,P=0.001)than those＜65 years old(OR=1.782,95％CI:1.076-2.949,P=0.025).Conclusion TyG index is closely associated with hypertension in patients with cerebral hemorrhage,especially in women and elderly.

OBJECTIVETo study the effect of supernatants from silicon dioxide(SiO2) stimulated pulmonary alveolar macrophages(PAM) on the localization of connexin 43(Cx43) so as to explore the inhibition level of SiO2 on alveolar epithelial cellular gap-junctional communication(GJIC).

METHODSThe supermatants from the primary cultured PAM were prepared, and then added 5% (v/v) SiO2 into 2% (v/v) NBS RPMI 1640 to stimulate the normal mink lung epithelial cell line CCL-64 for 24 hours. The localizations of Cx43 in CCL-64 were analyzed by indirect immunofluorescence histochemistry and laser confocal scanning microscopy(LCSM).

RESULTSThe normal cultured CCL-64 cells displayed bright membrane-associated Cx43 plaques labeling and formed dashes at regions of intercellular junction. Being exposed to supernatants from SiO2-stimulated PAM, the CCL-64 cells retained a relative low degree of Cx43 labeling at the cell periphery, localized in cytoplasm, and the individual spot, rather than plaques, were smaller compared to normal cultured cells. Along with the increase of the concentrations of SiO2, the cells displayed a different staining pattern, with clear cluster labeling aggregating towards the nucleus.

CONCLUSIONThe altered localization of the gap-junctional protein Cx43 in alveolar epithelial cells, mediated by SiO2, indicated that the internalization of Cx43 may contribute to the inhibition on GJIC in silica-induced lung epithelium injury.

Animals ; Cell Communication ; drug effects ; Cell Line ; Connexin 43 ; analysis ; Epithelial Cells ; chemistry ; drug effects ; Fluorescent Antibody Technique, Indirect ; Gap Junctions ; drug effects ; Microscopy, Confocal ; Mink ; Pulmonary Alveoli ; chemistry ; drug effects ; Silicon Dioxide ; toxicity

OBJECTIVETo explore the effect of silica dioxide(SiO2) on proliferation and downregulation of gap junctional intercellular communication (GJIC) in pulmonary alveolar epithelial cells (CCL-64 cells).

METHODSThe pulmonary alveolar macrophages(PAMs) were incubated in the serum-free RPMI 1640 containing the various concentration of SiO2 for 24 hours. The supernatants were prepared and added 5% (V/V) into 2% (V/V) NBS RPMI 1640 to stimulate the proliferation of CCL-64 cells for 24 hours. A set of "blank control", run in parallel, contained RPMI 1640 + 2% (V/V) NBS alone. The proliferation of CCL-64 cells was detected using MTT assay(to show as the absorbency, A570nm). GJIC function was measured using the fluorescence redistribution after photobleaching(FRAP) assay [to express as the transfer rate of the fluorescence, K (x 10(-3)/s)], with a laser scanning confocal microscope(LSCM, Leica TCS SP).

RESULTSThe silica-exposed PAM supernatants could induce both the proliferation(F = 9.679, P < 0.01) and downregulation of GJIC(F = 20.587, P < 0.01) of CCL-64 cells. In the range of 50-500 micrograms/ml SiO2 concentrations, the proliferation (A570nm values) and GJIC(the transfer rate, K) were fitted well in a dose-dependent manner(proliferation: r = 0.891, P < 0.05; GJIC: r = -0.943, P < 0.05).

CONCLUSIONBy way of stimulating the PAM, SiO2 could inhibit GJIC function in lung alveolar epithelial cells, and induce epithelial cell proliferation. In the pathogenesis of silicosis, the downregulation of GJIC of the pulmonary epithelial cells may play an important role in silica-mediated alveolar epithelial cell injury.

Cell Communication ; drug effects ; Cell Proliferation ; drug effects ; Epithelial Cells ; drug effects ; Gap Junctions ; drug effects ; Pulmonary Alveoli ; drug effects ; Silicon Dioxide ; toxicity ; Silicosis ; etiology

OBJECTIVETo express and identify soluble liver antigen (SLA) and cytochrome P-450 (CYP 2D6).

METHODSSLA cDNA and CYP 2D6 cDNA were obtained from human liver tissue poly (A)+RNA by RT-PCR. The cDNAs were inserted into fusion expression vector PQE-30 site of BamH I and Hind III. SLA and CYP 2D6 were identified by the SDS-PAGE and Western blot.

RESULTSSDS-PAGE analysis showed that there was a very strong stained band at about 47 kd and 50 kd, respectively. The products could specifically band to anti-SLA or anti-CYP 2D6 autoantibodies.

CONCLUSIONSThe clone and expression of SLA and CYP 2D6 provide useful substances for the diagnosis and research of pathogenesis on autoimmune hepatitis.

Autoantigens ; genetics ; Cytochrome P-450 Enzyme System ; genetics ; DNA, Complementary ; genetics ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; genetics ; Gene Expression ; Hepatitis, Autoimmune ; genetics ; Humans ; Liver ; metabolism ; Recombinant Fusion Proteins ; genetics ; metabolism

Objective　To explore the relationships betw een pulmonary fibrosis and downregulation of gap junctional intercellular comm unication(GJIC) of the fibroblasts after stimulation by SiO2. Metho ds　The pulmonary alveolar macrophage(PAM) and the phorbol 12-myrist ate 13-acetate(PMA or TPA) primed THP-1(pTHP-1),a monocyte-like cell line wi th the properties of PAM,were incubated in the serum-free RPMI?1640 containing SiO2 at various concentrations.The proliferation of Chinese hamster lung(CHL) fibroblast induced by cultured PAM or pTHP supernatant was detected by using MT T assay(to show as the a bsorbency,A570 nm),and GJIC between those cells was measured by using the fluorescence redis tribution after photobleaching(FRAP) assay(to show as the transfer rate of the f luorescence,K×10-3/s) performed with a laser scanning confocal micr oscope(LSCM,Carl Zeiss LSM 510,release 2.01). Results　Bot h SiO2 exposed PAM and pTHP-1 supern atants could induce the proliferation(PAM:F=3.205,P<0.05;pTHP-1:F=1 3.779,P<0.01) and the downregulation of GJIC(PAM:F=19.948,P<0. 01;pTHP-1:F=9.365,P<0.01) of the CHL cells.In the range of 0,50,1 00,200 and 500 μg/ml SiO2 concentrations,the proliferation(A570 nm values) and GJIC(the transfer rate,K)were fitted well in the dose-effect re lationship(PAM:r=-0.803,P<0.05;pTHP-1：r=-0.914,P<0.01). Compared with the blank control,both PAM and pTHP-1 supernatants could upregu late GJIC(K:21.24×10-3/s,18.92×10-3/s vs. 7.81×10 -3/s,7.81×10-3/s respectively,P<0.05) and inh ibit the proliferation of CHL cell(A570 nm:0.506,0.218 vs. 0.5 39,0.388 respectively,P<0.05). Conclusion　T hrough the way of stimulating PAM,SiO2 could inhibit GJIC of fibroblasts,and induce fibroblast proliferation.In the pathoge nesis of silicotic fibrosis,the downregulation of GJIC of the pulmonary fibrobla sts may play an important role.

Objective　To explore the relationships betw een pulmonary fibrosis and downregulation of gap junctional intercellular comm unication(GJIC) of the fibroblasts after stimulation by SiO2. Metho ds　The pulmonary alveolar macrophage(PAM) and the phorbol 12-myrist ate 13-acetate(PMA or TPA) primed THP-1(pTHP-1),a monocyte-like cell line wi th the properties of PAM,were incubated in the serum-free RPMI?1640 containing SiO2 at various concentrations.The proliferation of Chinese hamster lung(CHL) fibroblast induced by cultured PAM or pTHP supernatant was detected by using MT T assay(to show as the a bsorbency,A570 nm),and GJIC between those cells was measured by using the fluorescence redis tribution after photobleaching(FRAP) assay(to show as the transfer rate of the f luorescence,K×10-3/s) performed with a laser scanning confocal micr oscope(LSCM,Carl Zeiss LSM 510,release 2.01). Results　Bot h SiO2 exposed PAM and pTHP-1 supern atants could induce the proliferation(PAM:F=3.205,P<0.05;pTHP-1:F=1 3.779,P<0.01) and the downregulation of GJIC(PAM:F=19.948,P<0. 01;pTHP-1:F=9.365,P<0.01) of the CHL cells.In the range of 0,50,1 00,200 and 500 μg/ml SiO2 concentrations,the proliferation(A570 nm values) and GJIC(the transfer rate,K)were fitted well in the dose-effect re lationship(PAM:r=-0.803,P<0.05;pTHP-1：r=-0.914,P<0.01). Compared with the blank control,both PAM and pTHP-1 supernatants could upregu late GJIC(K:21.24×10-3/s,18.92×10-3/s vs. 7.81×10 -3/s,7.81×10-3/s respectively,P<0.05) and inh ibit the proliferation of CHL cell(A570 nm:0.506,0.218 vs. 0.5 39,0.388 respectively,P<0.05). Conclusion　T hrough the way of stimulating PAM,SiO2 could inhibit GJIC of fibroblasts,and induce fibroblast proliferation.In the pathoge nesis of silicotic fibrosis,the downregulation of GJIC of the pulmonary fibrobla sts may play an important role.