Objective:To investigate the feasibility and clinical value of artificial intelligence-assisted compressed sensing (ACS) technology in accelerating MR simulation (MR-sim) for radiotherapy of nasopharyngeal carcinoma (NPC).Methods:Thirty patients with NPC scheduled to receive radical radiotherapy at Sun Yat-sen University Cancer Center were prospectively enrolled. All patients underwent head and neck MR-sim on a 3.0 T scanner, with axial T 1 weighted imaging (WI), T 2WI, contrast-enhanced T 1WI, and fat-suppressed contrast-enhanced T 1WI images acquired using both ACS and parallel imaging (PI) techniques. Paired-sample t tests or rank-sum tests were used to compare scan time, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) of MR-sim images between the two techniques. A 5-point Likert scale was applied to evaluate tumor lesion visualization, lesion margin clarity, artifacts, and overall image quality, with chi-square tests used to compare subjective image quality scores between the two techniques. Tumor target volumes were delineated on MR-sim images obtained by both ACS and PI techniques after fusion with CT simulation images, and consistency was assessed using the Dice similarity coefficient (DSC). Results:For both individual sequences and overall protocols, ACS significantly reduced MR-sim acquisition time compared with PI ( P < 0.001). The total acquisition time with ACS was (378.60±17.07) s versus (694.93±17.07) s with PI, representing a 45.52% time reduction. SNR, CNR, tumor lesion identification, margin clarity, artifacts, and overall image quality scores of MR-sim images did not differ significantly between ACS and PI ( P > 0.05). Tumor target volumes delineated from ACS- and PI-based MR-sim images showed high consistency after fusion with CT simulation images ( P > 0.05), with mean DSC values of primary tumors and metastatic cervical lymph nodes approaching 1. Conclusion:Compared with conventional MR acceleration methods (PI), ACS enables faster MR-sim acquisition in NPC without compromising image quality or the accuracy of tumor target delineation.

Objective To investigate the predictive value of maximum thickness of carotid plaque combined with triglyceride glucose(TyG)index for early neurological deterioration(END)in elderly patients with hypertension combined with acute cerebral infarction(ACI).Methods A prospective study was conducted on 120 elderly hypertensive patients with concomitant ACI admitted in our hospital between October 2022 and October 2024.According to the occurrence of END or not,they were divided into an END group(48 cases)and a non-END group(72 cases).The clinical data and ultrasound parameters were collected in all patients.Multivariate logistic regression analysis was used to determine the factors influencing the occurrence of END.ROC curve was plotted to assess the predictive value of the maximum thickness of the carotid plaque and the TyG index for END.Results The END group exhibited significantly higher SBP at admission,higher low-density lipoprotein cholesterol,TyG index and thicker maximum thickness of carotid plaque than the non-END group(P＜0.05,P＜0.01).TyG index and maximum thickness of carotid plaque were influencing factors for END occurrence in elderly hypertension patients with ACI.The AUC value of TyG index,carotid plaque maximum thickness and their combination for predicting the occurrence of END was 0.801,0.774 and 0.911,respectively,with the combination showing better predictive efficiency(P＜0.01).Conclusion TyG index and maximum thickness of carotid plaque are influencing factors for END in elderly patients with hypertension combined with ACI,and their combination has better predictive value for END in the patients.

Objective To investigate the predictive value of maximum thickness of carotid plaque combined with triglyceride glucose(TyG)index for early neurological deterioration(END)in elderly patients with hypertension combined with acute cerebral infarction(ACI).Methods A prospective study was conducted on 120 elderly hypertensive patients with concomitant ACI admitted in our hospital between October 2022 and October 2024.According to the occurrence of END or not,they were divided into an END group(48 cases)and a non-END group(72 cases).The clinical data and ultrasound parameters were collected in all patients.Multivariate logistic regression analysis was used to determine the factors influencing the occurrence of END.ROC curve was plotted to assess the predictive value of the maximum thickness of the carotid plaque and the TyG index for END.Results The END group exhibited significantly higher SBP at admission,higher low-density lipoprotein cholesterol,TyG index and thicker maximum thickness of carotid plaque than the non-END group(P＜0.05,P＜0.01).TyG index and maximum thickness of carotid plaque were influencing factors for END occurrence in elderly hypertension patients with ACI.The AUC value of TyG index,carotid plaque maximum thickness and their combination for predicting the occurrence of END was 0.801,0.774 and 0.911,respectively,with the combination showing better predictive efficiency(P＜0.01).Conclusion TyG index and maximum thickness of carotid plaque are influencing factors for END in elderly patients with hypertension combined with ACI,and their combination has better predictive value for END in the patients.

Objective:To investigate the feasibility and clinical value of artificial intelligence-assisted compressed sensing (ACS) technology in accelerating MR simulation (MR-sim) for radiotherapy of nasopharyngeal carcinoma (NPC).Methods:Thirty patients with NPC scheduled to receive radical radiotherapy at Sun Yat-sen University Cancer Center were prospectively enrolled. All patients underwent head and neck MR-sim on a 3.0 T scanner, with axial T 1 weighted imaging (WI), T 2WI, contrast-enhanced T 1WI, and fat-suppressed contrast-enhanced T 1WI images acquired using both ACS and parallel imaging (PI) techniques. Paired-sample t tests or rank-sum tests were used to compare scan time, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) of MR-sim images between the two techniques. A 5-point Likert scale was applied to evaluate tumor lesion visualization, lesion margin clarity, artifacts, and overall image quality, with chi-square tests used to compare subjective image quality scores between the two techniques. Tumor target volumes were delineated on MR-sim images obtained by both ACS and PI techniques after fusion with CT simulation images, and consistency was assessed using the Dice similarity coefficient (DSC). Results:For both individual sequences and overall protocols, ACS significantly reduced MR-sim acquisition time compared with PI ( P < 0.001). The total acquisition time with ACS was (378.60±17.07) s versus (694.93±17.07) s with PI, representing a 45.52% time reduction. SNR, CNR, tumor lesion identification, margin clarity, artifacts, and overall image quality scores of MR-sim images did not differ significantly between ACS and PI ( P > 0.05). Tumor target volumes delineated from ACS- and PI-based MR-sim images showed high consistency after fusion with CT simulation images ( P > 0.05), with mean DSC values of primary tumors and metastatic cervical lymph nodes approaching 1. Conclusion:Compared with conventional MR acceleration methods (PI), ACS enables faster MR-sim acquisition in NPC without compromising image quality or the accuracy of tumor target delineation.

OBJECTIVETo observe the regulative effects of the acupuncture on glucose and lipid metabolism disorder in the patients of metabolic syndrome.

METHODSSeventy-six patients of metabolic syndrome were rando-mized into an acupuncture plus western medicine group (37 cases) and a western medicine group (39 cases). In the western medicine group, the conventional western medication was used for 40 days. In the acupuncture plus western medicine group, the acupuncture was combined on the basis of the treatment as the western medicine group, the acupoints were Danzhong (CV 17), Zhongwan (CV 12), Tianshu (ST 25), etc. Ten treatments were as one session. There were 3 to 5 days of intervals between the sessions and totally 30 treatments were required. The body mass index (BMI), blood lipid, blood glucose, and comprehensive therapeutic effects were compared before and after treatment in the two groups.

RESULTSBefore and after treatment, the differences were all significant in BMI, triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), fasting blood glucose (FBG), plasma glucose of 2 hours post glucose-load (2 hPG), fasting insulin (FINS) and insulin resistance index (HOMA-IR) (all<0.05) in the acupuncture plus western medicine group, and the results after treatment were superior to those before treatment; the difference was not significant in BMI (>0.05) and those were all significant statistically in TG, TC, LDL-C, HDL-C, FBG, 2 hPG, FINS, HOMA-IR (all<0.05) in the western medicine group, and the results after treatment were superior to those before treatment. After treatment, in comparison of the two groups, the results in the acupuncture plus western medicine group were better than those in the western medicine group. The differences were all signif-icant sta-tistically in BMI, TG, TC, LDL-C, HDL-C, FBG, 2 hPG, FINS, HOMA-IR (all<0.05).

CONCLUSIONSOn the basis of the conventional western medicine, the acupuncture relieves apparently the clinical symptoms and rectifies glucose and lipid metabolism disorder in the patients of metabolic syndrome.

Objective To establish a stable lung cancer A549 cell line transfected by AMP-activated protein kinase (AMPK) expression vector,and to observe the effect of AMPK on proliferation as well as on the invasive ability of A549 cells.Methods Full-length of AMPK gene was amplified and its target gene was digested,then inserted into the GV358 plasmid.Co-tranfected 293T cells were subjected to the lentivirus equipment package.Subsequently,we collected the lentivirus supernatant to infect the A549 cells and establish a stably,overexpressed cell line A549.The mRNA and protein of AMPK were examined by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blotting.The proliferation and invasion abilities of A549 cells were detected by methyl thiazolyl thiazolium (MTT) and Transwell assay.Results GV358-AMPK lentivirus vectors was successfully constructed by restrictive enzyme digestion and plasmid sequencing.There were significantly increased expressions of AMPK protein (5.87 times,P =0.002) and mRNA (16.12 times,P ＜ 0.001) after transfected with GV358-AMPK compared with the Vector group.Meanwhile,AMPK overexpression showed significantly lower proliferation (the forth day:0.53 ± 0.03 vs.0.64 ±0.05,P=0.021;the fifth day:0.58 ± 0.04 vs.0.80 ± 0.07,P =0.002) and weaken invasive ability [(1.6±0.5) ×l05 vs.(3.4±0.3) ×105,P=0.004] ofA549 cells.Conclusion The lentiviralAMPK expression vector and its A549 cell line is successfully constructed.AMPK overexpression inhibits the proliferation and invasive ability of A549 cells.

Arthroplasty, Replacement, Knee ; adverse effects ; Female ; Humans ; Knee Joint ; diagnostic imaging ; surgery ; Middle Aged ; Radiography

Objective:A study was conducted to determine the expression of acetyl-coa carboxylase product of phosphorylation (P-ACC) and an enzyme called cyclooxygenase 2 (COX-2) in non-small cell lung cancer (NSCLC) tissue, as well as the relationship and correlations between tumor size, lymph node metastasis, clinical stage, and pathological type. Methods: Sixty-two patients with NSCLC lung cancer tissues were included in the patient group, whereas 20 patients who underwent lobectomy for other reasons and had normal lung tissues were included in the control group. Immunohistochemical streptavidin peroxidase method was used to detect the expression of P-ACC and COX-2 in lung cancer and normal lung tissues. Results:The positive expressions of P-ACC and COX-2 in NSCLC lung cancer and normal lung tissues were significantly different (P<0.05). In NSCLC tissues, the positive expression of P-ACC was significantly associated with tumor size (P<0.05), but was not significantly associated with lymph node metastasis, clinical stage, and pathological type. We found no correlation between the positive expression of COX-2 and tumor size, lymph node metasta-sis, clinical stage and pathological type. Further analysis revealed that the positive expression of P-ACC and COX-2 in NSCLC was sig-nificantly and negatively correlated (r=-2.37, P=0.032). Conclusion:The positive expression of COX-2 in NSCLC greatly increased compared with that of P-ACC, and a significantly negative correlation was observed between them. We propose that the positive expres-sion of P-ACC reduction may activate the positive expression of COX-2 and promote the occurrence, development, invasion, and metas-tasis of NSCLC.

Objective To develop a method to determine Pb and Cd in Qingkailing Injection by graphite furnace atomic absorption spectrometry, and search their source. Methods The samples, digested via microwave, were determined the contents of Pb and Cd by graphite furnace atomic absorption spectrometry in raw material, intermediate and finished product of Qingkailing Injection. Results The standard curve of Pb was Y=0.007 3X+0.011 6, and Cd was Y=0.056 7X+0.060 4. The regression equation of Pb and Cd was 97.0% and 95.6%, respectively. Content determination of Pb and Cd in Qingkailing Injection revealed that Pb and Cd in finished product came from raw materials. Conclusion The method is rapid, high sensitive and accurate, and can be applied to the inspection of Pb and Cd in Qingkailing Injection.

Objective To explore the possibility of predicting postnatal depression based on Hospital Anxiety and Depression (HAD) scale for women during the third trimester of pregnancy.Methods 1 230 pregnant women were evaluated during their third trimester of pregnancy using HAD scale deriving from common questionnaire and self-related questionnaire,and again 4 weeks postnatal using Edinburgh Postnatal Depression Scale (EPDS).Results (1) The incidences for depression and anxiety during the third trimester of pregnancy were 5.20％ (64 /1 230) and 4.07％ (50 /1 230),respectively.The incidence of postnatal depression was 9.98％ (121 /1 213).(2) According to the two step screening diagnosis for severe postnatal depression,the 1 230 cases were categorized into patient group (121 cases) and normal group (1 092 cases).There were statistically significant differences between the two groups in age,the relationship of the couple,work and study pressure,knowledge of childbirth,parenting and postnatal depression,maternal gender discrimination,group HAD score and EPDS score (x2 =6.54,6.92,8.07,23.82,21.88,10.24,9.26,6.63,5.66,P ＜ 0.05 for all).There were no significant differences between the two groups in marital status,personality,level of education,occupation,history of abnormal delivery,complications of pregnancy,gender discrimination from the husband and/or family.(3) In ROC curve the optimal critical value of the HAD during postnatal depression was 9 symptoms.For the cases who got an A or D in the HAD scale,the sensitivity and specificity of predicting postnatal depression were 86.21％/87.64％,95.43％/94.72％,respectively.(4) For the cases who got an A or D in the HAD scale,the area under the ROC curve were 0.941 and 0.914,respectively.(5) The optimal critical value of HAD was related to the scores of EPDS after delivery (r =0.852,0.846,both P ＜ 0.01).(6) For the cases with HAD ≥ 9 points,the probability for the risk of postnatal depression will be increased by 15.891 and 18.264 times,respectively.Conclusion HAD scale can be used as a routine screening method for women in late stage pregnancy.Women with HAD ≥ 9 points should be comprehensively evaluated for the risk of postnatal depression.