Objective To analyze the relationship between serum micro ribonucleic acid(miRNA)-214,cholinesterase(ChE),albumin(ALB)levels and the prognosis of patients with acute organophosphate poison-ing(AOPP).Methods A total of 160 patients with AOPP admitted to the hospital were enrolled as the re-search objects between June 2018 and December 2022.According to whether they were alive at 14 d after treatment,patients were divided into survival group(119 cases)and death group(41 cases).The relative ex-pression level of serum miRNA-214 was detected by fluorescence quantitative polymerase chain reaction,ChE level was detected by full-automatic biochemical analyzer,and level of serum ALB was detected by immuno-turbidimetry.The correlation between serum miRNA-214 and ChE,ALB in AOPP patients was analyzed by Pearson correlation analysis.The risk factors of prognosis in AOPP patients were analyzed by Logistic regres-sion analysis.The predictive efficiency of acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)score,miRNA-214,ChE and ALB for prognosis was detected by receiver operating characteristic(ROC)curves.Results APACHE Ⅱ score and relative expression level of serum miRNA-214 in death group were significantly higher than those in survival group,while levels of ChE and ALB were significantly lower than those in survival group(P＜0.05).The results of Logistic regression analysis showed that miRNA-2143.75 and ChE＜931.92 U/L were all independent risk factors of poor prognosis in AOPP patients(P＜0.05).Pearson correlation analysis showed that miRNA-214 was not correlated with ChE and ALB in death group(r=-0.172,0.035,P＞0.05).The results of ROC curves showed that area under the curve(AUC)values of APACHE Ⅱ score,miRNA-214,ChE and ALB for predicting poor prognosis of AOPP patients were 0.777,0.933,0.805 and 0.881,respectively.Conclusion The serum miRNA-214,ChE and ALB levels are re-lated to prognosis of AOPP patients,which can be applied as important reference indexes to predict prognosis of AOPP patients.

Methods and significances of the laboratory opening management for the function experiment teaching center are introduced in this paper. The problems existing in the management of the open Lab and the thinking about the management are communicated with the peers to provide the reference for the opened lab.

To discuss the course of implementing tutorial system as well as the effect on medical students,and to point out that implementing teacher system is a kind of pattern of training innovation type of medical talents.

In order to make good use of laboratory opening,the way,method together with the effect of laboratory opening in our college were analyzed and discussed in this paper. The perfect practice effect shows that laboratory opening plays an important role in cultivation for independent research abilities and scientific innovative abilities of undergraduates.

BACKGROUND: Previously, there are some researches on studying the effects of hypoxia or hypothermia singly. Nowadays, study on the synthetic action of hypoxia and hypothermia has been attached widely importance.OBJECTIVE: To explore the combination effects of acute hypothermia and hypoxia on superoxide dismutase(SOD) and malondialdehyde in normal rabbits and rabbits with myocardial ischemia.DESIGN: Randomized controlled trial.SETTING, MATERIALS and INTERVENTIONS: Experimental site:Physiological Laboratory of Youjiang Ethnic Medical College. A total of 32 healthy rabbits weighed between 1.8to 2.5 kg were chosen. They were randomly divided into 4 groups each with 8 rabbits: normal group, hypothermia and hypoxia group, ischemic group with normal temperature and oxygen,ischemic group with hypothermia and hypoxia. Normal rabbits and ischemic rabbits were put in fridge at low temperature( -10 ± 2) ℃ and low oxygen (oxygen content of 8.5% ) for one hour and tested activity of SOD and concentration of malondialdehyde in serum.MAIN OUTCOME MEASURES: Comparison of serum SOD and malondialdehyde between normal rabbits and myocardial ischemic rabbits under the acute hypothermia and hypoxia conditions.RESULTS: The SOD level of rabbits in hypothermia and hypoxia group [(371.04 ± 29.96) kNU/L] was much lower than that of normal temperature and oxygen group[ (424.09 ± 22.59) kNU/L] ( t = 5.21, P < 0.01) while the content of malondialdehyde[ (5.58 ± 0. 44) μ mol/L] was greater than that of normal group[ (4.44 ± 1.11) μ mol/L] ( t = 3.21, P < 0.05) . In myocardial ischemia group with hypothermia and hypoxia, SOD was greatly lower than that of myocardial ischemia group with normal temperature and oxygen(t=4.37, P < 0.01) while the malondialdehyde was greatly higher than that of ischemic group with normal temperature and oxygen group(t=2.94, P <0.05).CONCLUSION: The combined effects of acute hypothermia and hypoxia will aggravate the damage to body.

We provide laboratory for students to design and complete experiment independently,and examine their operation ability in course of experiment.After accomplishing their experiments,the students must write a disquisition on it.Through this exploration experimentation,we have trained students scientific analysis ability and innovation ability and make them benefit form it in their future study and work.