To thoroughly implement the strategic deployment outlined in the Opinions of the Central Committee of the Communist Party of China and the State Council on Promoting the Inheritance and Innovative Development of Traditional Chinese Medicine regarding research on dominant diseases of traditional Chinese medicine and to uphold the development philosophy of equal emphasis on traditional Chinese medicine and western medicine，the China Association of Chinese Medicine has fully played a leading academic role by systematically organizing and conducting a series of academic youth salons on clinical dominant diseases of traditional Chinese medicine. On September 13，2024，the 36^th Youth Salon on Clinical Dominant Diseases was successfully held in Nanjing，focusing on the advantages of traditional Chinese medicine and the integrative traditional Chinese medicine and western medicine in the diagnosis and treatment of erectile dysfunction （ED）. The conference brought together leading experts from traditional Chinese medicine，western medicine，and interdisciplinary fields，facilitating in-depth multidisciplinary discussions that led to key consensus on optimizing traditional Chinese medicine treatment protocols for ED，researching and developing new drugs of traditional Chinese medicine，and advancing interdisciplinary development in traditional Chinese medicine. This salon systematically sorted out the clinical strengths and distinctive features of traditional Chinese medicine in the diagnosis and treatment of ED. Based on current research foundations and clinical needs，it identified key directions for future scientific layout and scientific research tackling： （1） Standardization of syndrome differentiation system of traditional Chinese medicine for ED. （2） Optimization and standardization of intervention methods of integrated traditional Chinese medicine and western medicine. （3） High-quality clinical research guided by evidence-based medicine. （4） In-depth analysis of the pharmacological mechanisms of traditional Chinese medicine in the treatment of ED. （5） Clinical translation and application promotion of new drugs of traditional Chinese medicine. （6） Interdisciplinary integration and innovation in traditional Chinese medicine. For each research direction，key focus areas，expected objectives，and clinical value were further refined，along with the establishment of a scientifically sound priority funding level evaluation system. Therefore，building on the series of salons on the ED-focused dominant diseases of traditional Chinese medicine，this paper provides standardized guidance for clinical practice of traditional Chinese medicine in ED management，effectively contributing to the high-quality development of traditional Chinese medicine. It serves as a valuable reference for national scientific and technological strategic layout， research and development decision-making in new drugs of traditional Chinese medicine，research topic planning，and clinical guideline formulation.

Objective To compare and analyze the germicidal efficacy of continuous ultraviolet and pulsed ultravio-let(UV)lasers on pathogenic microorganisms.Methods Spore slides of Escherichia coli and Bacillus atrophaeus were irradiated using a 254 nm ultraviolet mercury lamp and UV laser.The carrier quantitative germicidal test was conducted to determine the disinfection efficacy at different irradiation doses.Results When the irradiation dose of the ultraviolet mercury lamp was 245.52 mJ/cm2,the logarithmic killing values of Escherichia coli and Bacillus atro-phaeus spores were 5.00 and 2.92,respectively,and the mean logarithmic killing doses were 49.10 and 84.08 mJ/cm2,respectively;When the UV laser irradiation doses were 208.39 and 206.80 mJ/cm2,the logarithmic killing values for the two microorganisms were 6.29 and 3.32,respectively,and the mean logarithmic killing doses were 33.13 and 62.29 mJ/cm2,respectively.Conclusion Compared with continuous UV radiation,pulsed UV laser has stron-ger penetration ability,better killing efficacy on pathogenic microorganisms at the same radiation dose,and can con-duct targeted disinfection and sterilization on the surface of objects directionally.

Objective To compare and analyze the germicidal efficacy of continuous ultraviolet and pulsed ultravio-let(UV)lasers on pathogenic microorganisms.Methods Spore slides of Escherichia coli and Bacillus atrophaeus were irradiated using a 254 nm ultraviolet mercury lamp and UV laser.The carrier quantitative germicidal test was conducted to determine the disinfection efficacy at different irradiation doses.Results When the irradiation dose of the ultraviolet mercury lamp was 245.52 mJ/cm2,the logarithmic killing values of Escherichia coli and Bacillus atro-phaeus spores were 5.00 and 2.92,respectively,and the mean logarithmic killing doses were 49.10 and 84.08 mJ/cm2,respectively;When the UV laser irradiation doses were 208.39 and 206.80 mJ/cm2,the logarithmic killing values for the two microorganisms were 6.29 and 3.32,respectively,and the mean logarithmic killing doses were 33.13 and 62.29 mJ/cm2,respectively.Conclusion Compared with continuous UV radiation,pulsed UV laser has stron-ger penetration ability,better killing efficacy on pathogenic microorganisms at the same radiation dose,and can con-duct targeted disinfection and sterilization on the surface of objects directionally.

Objective To evaluate the reliability and validity of the Chinese version of the hyperacusis scale and estimate its applicability in the Chinese population.Methods The patients admitted to the Department of Oto-laryngology of Xijing Hospital from June 2017 to December 2017 were surveyed.A total of 300 questionnaires were sent out and 293 valid questionnaires were collected with effective response rate of 97.67％.All participants comple-ted a basic information survey,pure tone audiometry and fill in the Chinese version of the hyperacusis scale.The re-liability and validity of this scale were evaluated.Results Among the 293 respondents,243(82.93％)were compli-cated with tinnitus.A total of 181 cases(61.77％)were subjectively diagnosed with hyperacusis through question-naire survey.According to the scale evaluation,174 cases(59.39％)were diagnosed with hyperacusis,and the Kappa value of conformity test of the two methods was 0.81(P＜0.001).Reliability analysis indicated a Cronbach'sα coefficient of 0.93 for the entire scale.Each dimension had a Cronbach's α coefficient＞0.60.The split-half relia-bility coefficient for the entire scale was 0.86,and for each dimension,it was＞0.60.Exploratory factor analysis of validity suggested that 25 items could be grouped under 4 factors,such as functional part,social part,emotional part and harmonic sensitivity part.These factors had eigenvalues greater than 1,accounting for 59.42％of the total variance.Post-rotation,the 25 items were distributed across the 4 components,with each item having a factor load-ing＞0.40.Confirmatory factor analysis of validity showed model fit parameters as:x2/df=1.98,GFI=0.91,AGFI=0.83,CFI=0.92,TLI=0.89,RMSEA=0.08.Each item had a standardized factor loading＞0.40.Con-clusion The Chinese version of the hyperacusis scale demonstrated strong reliability and validity,making it suitable for use among the Chinese population.

Patients and animal models suffering from acute pancreatitis(AP)have characteristic phenotypes of gut microbiota,which is associated with the severity of AP.Gut microbiota directly or indirectly participates in host immune regulation by catabolizing nutrients into functional products.The imbalance of gut microbiota and its metabolites activate the immune inflammatory response,which attribute to the aggravation of AP.According to the theory of traditional Chinese medicine,the abnormal metabolism and the release of inflammatory factors caused by the imbalance of gut microbiota are classified into the category of"endotoxin",which combine with external toxins(alcohol,food,cold poisoning,etc.)to turn into heat and even fire.Therefore,"microbiota-metabolism-immunity"axis disorder is the molecular biological basis for the"toxin and heat"theory in AP.For traditional Chinese medicine,classical prescriptions and proven prescriptions could treat AP by targeting to regulate the"microbiota-metabolism-immunity"axis,which not only enriches the"toxic and heat"theory,but also provides new ideas for the prevention of AP.

OBJECTIVE To explore the construction of the physical identification model of twenty-five people of yin and yang by combining the hand diagnosis image analysis of machine learning,in order to enrich the input of physical identification features.METHODS Based on the theory of twenty-five people of yin and yang,the hand images of 542 patients with cardiovascular disease were collected by self-developed hand diagnosis acquisition equipment.The key points of the hand were extracted by the Mediapipe palm key point algorithm,and the palm area was segmented by geometry to obtain the length and color gamut characteristics of different parts.The physician with clinical license was used to determined the patient's yin and yang twenty-five constitution according to the e-lectronic medical record,and the patient's constitution was identified by comparing Random Forest,Logistic Regression,Xgboost algo-rithm and Light GBM.RESULTS This study successfully constructed a TCM constitution identification model based on hand charac-teristics,which can identify ten basic constitution types including gold wood,gold fire,gold water,gold soil,wood fire,wood water,wood soil,water fire,water soil and fire soil.After sample equalization,the Random Forest model performed best in physical identifi-cation,with an accuracy rate of 0.69,which was significantly better than other models.CONCLUSION The constitution identifica-tion model of TCM hand diagnosis based on hand characteristics can realize the constitution identification of the five elements of TCM.However,due to the use of only hand diagnosis data of TCM,the accuracy needs to be improved.Compared with the traditional body mass scale,it is more objective,convenient,highly stable and consistent.In the future,multimodal integration with face diagno-sis and whole body inspection can be carried out to promote the accuracy,intelligence and multi-dimensional evaluation ability of TCM constitution identification technology.

OBJECTIVE To explore the construction of the physical identification model of twenty-five people of yin and yang by combining the hand diagnosis image analysis of machine learning,in order to enrich the input of physical identification features.METHODS Based on the theory of twenty-five people of yin and yang,the hand images of 542 patients with cardiovascular disease were collected by self-developed hand diagnosis acquisition equipment.The key points of the hand were extracted by the Mediapipe palm key point algorithm,and the palm area was segmented by geometry to obtain the length and color gamut characteristics of different parts.The physician with clinical license was used to determined the patient's yin and yang twenty-five constitution according to the e-lectronic medical record,and the patient's constitution was identified by comparing Random Forest,Logistic Regression,Xgboost algo-rithm and Light GBM.RESULTS This study successfully constructed a TCM constitution identification model based on hand charac-teristics,which can identify ten basic constitution types including gold wood,gold fire,gold water,gold soil,wood fire,wood water,wood soil,water fire,water soil and fire soil.After sample equalization,the Random Forest model performed best in physical identifi-cation,with an accuracy rate of 0.69,which was significantly better than other models.CONCLUSION The constitution identifica-tion model of TCM hand diagnosis based on hand characteristics can realize the constitution identification of the five elements of TCM.However,due to the use of only hand diagnosis data of TCM,the accuracy needs to be improved.Compared with the traditional body mass scale,it is more objective,convenient,highly stable and consistent.In the future,multimodal integration with face diagno-sis and whole body inspection can be carried out to promote the accuracy,intelligence and multi-dimensional evaluation ability of TCM constitution identification technology.

Patients and animal models suffering from acute pancreatitis(AP)have characteristic phenotypes of gut microbiota,which is associated with the severity of AP.Gut microbiota directly or indirectly participates in host immune regulation by catabolizing nutrients into functional products.The imbalance of gut microbiota and its metabolites activate the immune inflammatory response,which attribute to the aggravation of AP.According to the theory of traditional Chinese medicine,the abnormal metabolism and the release of inflammatory factors caused by the imbalance of gut microbiota are classified into the category of"endotoxin",which combine with external toxins(alcohol,food,cold poisoning,etc.)to turn into heat and even fire.Therefore,"microbiota-metabolism-immunity"axis disorder is the molecular biological basis for the"toxin and heat"theory in AP.For traditional Chinese medicine,classical prescriptions and proven prescriptions could treat AP by targeting to regulate the"microbiota-metabolism-immunity"axis,which not only enriches the"toxic and heat"theory,but also provides new ideas for the prevention of AP.

We prepared 15 batches of Kaixin Powder benchmark samples with the decoction pieces of different batches. Further, we established the specific chromatograms and index component content determination method of Kaixin Powder benchmark samples and analyzed the peaks and similarity of the chromatograms. With sibiricose A5, sibiricose A6, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, ginsenoside Rb_1, β-asarone, α-asarone, and dehydropachymic acid as index components, the index component content determination method was established and 70%-130% of the mean content of each component was set as the range. The chromatograms of 15 batches of Kaixin Powder benchmark samples had a total of 22 characteristic peaks, among which 8 peaks were identified, which represented sibiricose A5, sibiricose A6, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, ginsenoside Rb_1, β-asarone, α-asarone, and dehydropachymic acid, respectively. The chromatograms shared the similarity of 0.992-0.999. The 15 batches of benchmark samples had sibiricose A5 of 0.34-0.55 mg·g~(-1), sibiricose A6 of 0.43-0.57 mg·g~(-1), polygalaxanthone Ⅲ of 0.12-0.19 mg·g~(-1), 3,6'-disinapoyl sucrose of 1.08-1.78 mg·g~(-1), ginsenoside Rb_1 of 0.33-0.62 mg·g~(-1), β-asarone of 2.34-3.72 mg·g~(-1), α-asarone of 0.11-0.22 mg·g~(-1), and dehydropachymic acid of 0.053-0.079 mg·g~(-1). This study established the specific chromatograms and index component content determination method of Kaixin Powder benchmark samples, and the method was simple, feasible, reproducible, and stable. This study provides a scientific basis for further research on the key chemical properties of the benchmark samples and preparations of Kaixin Powder.
Powders ; Ginsenosides ; Benchmarking ; Drugs, Chinese Herbal/chemistry* ; Sucrose ; Chromatography, High Pressure Liquid/methods*

Rhizome rot is one of the main disease in the cultivation of Polygonatum cyrtonema, and it is also a global disease which seriously occurs on the perennial medicinal plants such as Panax notoginseng and P. ginseng. There is no effective control method at present. To identify the effects of three biocontrol microbes(Penicillium oxalicum QZ8, Trichoderma asperellum QZ2, and Brevibacillus amyloliquefaciens WK1) on the pathogens causing rhizome rot of P. cyrtonema, this study verified six suspected pathogens for their pathogenicity on P. cyrtonema. The result showed that Fusarium sp. HJ4, Colletotrichum sp. HJ4-1, and Phomopsis sp. HJ15 were the pathogens of rhizome rot of P. cyrtonema, and it was found for the first time that Phomopsis sp. could cause rhizome rot P. cyrtonema. Furthermore, the inhibitory effects of biocontrol microbes and their secondary metabolites on three pathogens were determined by confrontation culture. The results showed that the three tested biocontrol microbes significantly inhibited the growth of three pathogens. Moreover, the secondary metabolites of T. asperellum QZ2 and B. amyloliquefaciens WK1 showed significant inhibition against the three pathogens(P<0.05), and the effect of B. amyloliquefaciens WK1 sterile filtrate was significantly higher than that of high tempe-rature sterilized filtrate(P<0.05). B. amyloliquefaciens WK1 produced antibacterial metabolites to inhibit the growth of pathogens, and the growth inhibition rate of its sterile filtrate against three pathogens ranged from 87.84% to 93.14%. T. asperellum QZ2 inhibited the growth of pathogens through competition and antagonism, and P. oxalicum QZ8 exerted the inhibitory effect through competition. The research provides new ideas for the prevention and treatment of rhizome rot of P. cyrtonema and provides a basis for the di-sease control in other crops.
Polygonatum ; Rhizome