Objective To explore the expression and clinicopathological significance of secretory leukocyte protease inhibitor(SLPI)in cervical adenocarcinoma(CA)and adenocarcinoma in situ(AIS)of the cervix,and to analyze the correlation between SLPI expression and human papilloma virus(HPV)infection.Methods A total of 87 cases of cervical glandular lesions diagnosed in the Department of Pathology,the Affiliated Changsha Central Hospital,Hengyang Medical School,University of South China Changsha Central Hospital,from January 2019 to December 2022 were selected.Immunohistochemical staining of SLPI protein and HPV gene detection were performed on the tissues to explore the expression of SLPI in CA,AIS,and normal cervical glands,and to analyze the correlation between its expression level and the clinicopathological characteristics of CA patients and HPV infection.Results The expression of SLPI in AIS and CA tissues was lower than that in normal cervical tissues(P＜0.05).There was a negative correlation between the expression of SLPI and HPV infection(r=-0.353,P＜0.05).The expression of SLPI was significantly correlated with histological differentiation,HPV infection,vascular invasion,lymph node metastasis,Silva classification,and International Federation of Gynecology and Obstetrics staging of CA(P＜0.05).Conclusion SLPI is low-expressed in CA,which is closely related to the invasive biological and pathological characteristics.The low expression of SLPI is related to HPV infection,suggesting that SLPI may participate in the progression of CA as a tumor-suppressor factor.The decrease in SLPI expression may indicate HPV infection.SLPI is expected to become a potential biomarker for the diagnosis,treatment,and prognosis of CA.

Objective To explore the expression and clinicopathological significance of secretory leukocyte protease inhibitor(SLPI)in cervical adenocarcinoma(CA)and adenocarcinoma in situ(AIS)of the cervix,and to analyze the correlation between SLPI expression and human papilloma virus(HPV)infection.Methods A total of 87 cases of cervical glandular lesions diagnosed in the Department of Pathology,the Affiliated Changsha Central Hospital,Hengyang Medical School,University of South China Changsha Central Hospital,from January 2019 to December 2022 were selected.Immunohistochemical staining of SLPI protein and HPV gene detection were performed on the tissues to explore the expression of SLPI in CA,AIS,and normal cervical glands,and to analyze the correlation between its expression level and the clinicopathological characteristics of CA patients and HPV infection.Results The expression of SLPI in AIS and CA tissues was lower than that in normal cervical tissues(P＜0.05).There was a negative correlation between the expression of SLPI and HPV infection(r=-0.353,P＜0.05).The expression of SLPI was significantly correlated with histological differentiation,HPV infection,vascular invasion,lymph node metastasis,Silva classification,and International Federation of Gynecology and Obstetrics staging of CA(P＜0.05).Conclusion SLPI is low-expressed in CA,which is closely related to the invasive biological and pathological characteristics.The low expression of SLPI is related to HPV infection,suggesting that SLPI may participate in the progression of CA as a tumor-suppressor factor.The decrease in SLPI expression may indicate HPV infection.SLPI is expected to become a potential biomarker for the diagnosis,treatment,and prognosis of CA.

Objective: To observe the preventive effect of alkaline drinking water on hyperuricemia in mice. Methods: Sixty male SPF Kunming mice were randomly divided into six groups: pH 7.3, pH 8.0, pH 9.3 intervention groups, in which the mice were given water with pH values of 7.3±0.5, 8.0±0.5 and 9.3±0.6, respectively; the control group, model group and positive drug group ( with 2 g/L allopurinol ) were given double distilled water. Except for the control group, the mice in each group were given yeast by gavage (1.5 g/mL) for 13 days. On the 14th day, the mice were injected with 300 mg/kg potassium oxyzinate by intraperitoneal injection, and then fasted for 1 day. On the 16th day, serum uric acid, creatinine and urea nitrogen were detected, and renal tissues were stained to observe the morphology.The expression levels of neutrophil gelatinase-associated lipocalin ( NGAL ), tissue inhibitor of metalloproteinase 1( TIMP1 ), organic anion transporter 1 ( OAT1 ) and urate transporter 1 ( URAL-1 ) in renal tissues were determined bywestern blotting. The mRNA expression levels of URAL-1 and OAT1 were detected by real-time fluorescent quantita⁃tive polymerase chain reaction. Results: The level of serum uric acid was higher in the model group than in the control group and in the pH 9.3 intervention group (both P<0.05). The number and area of renal tubular lesions were less in the pH 9.3 intervention group than in the model group (all P<0.05). The relative expression levels of NGAL and URAT-1 proteins were lower in the pH 9.3 intervention group than in the model group, and the relative expression level of OAT1 protein was higher in the pH 9.3 intervention group than in the model group ( all P<0.05). The relativeexpression level of URAT-1 mRNA was lower in the pH 9.3 intervention group than in the model group, and the rela⁃tive expression level of OAT1 mRNA was higher in the pH 9.3 intervention group than in the model group ( all P<0.05 ). Conclusion Alkaline drinking water with pH value of 9.3±0.6 can effectively prevent hyperuricemia and acute kidney injury in mice.

Emotion is a series of reactions triggered by a specific object or situation that affects a person's physiological state and can, therefore, be identified by physiological signals. This paper proposes an emotion recognition model. Extracted the features of physiological signals such as photoplethysmography, galvanic skin response, respiration amplitude, and skin temperature. The SVM-RFE-CBR(Recursive Feature Elimination-Correlation Bias Reduction-Support Vector Machine) algorithm was performed to select features and support vector machines for classification. Finally, the model was implemented on the DEAP dataset for an emotion recognition experiment. In the rating scale of valence, arousal, and dominance, the accuracy rates of 73.5%, 81.3%, and 76.1% were obtained respectively. The result shows that emotional recognition can be effectively performed by combining a variety of physiological signals.
Arousal ; Emotions ; Galvanic Skin Response ; Humans ; Photoplethysmography ; Support Vector Machine

Objective:To investigate the diagnostic value of different detection methods for Mycobacterium tuberculosis in bronchoalveolar lavage fluid (BALF) from patients with pulmonary tuberculosis.Methods:BALF from l00 patients in Changsha Central Hospital from January 2013 to December 2015 was collected.Among 100 patients,65 cases were clinically diagnosed as tuberculosis,and 35 cases served as control.BALF smear method,polymerase chain reaction (PCR) and membrane reverse dot blot (RDB) were used for synchronous detection of Mycobacterium tuberculosis.Results:The positive rates by BALF smear method,PCR and RDB were 43.08％,73.84％ and 92.31％,respectively (P＜0.05).Sensitivity,specificity,accuracy,and negative predictive value for BALF smear were 43.08％,88.57％,59.00％,and 45.59％,respectively;for PCR were 73.85％,100％,83.00％,and 67.31％,respectively;for RDB were 92.31％,100.00％,95.00％,and 87.50％,respectively.Conclusion:The technique of membrane RDB can not only accurately diagnose Mycobacterium tuberculosis,but also can rapidly and easily identify the resistance of Mycobacterium tuberculosis to streptomycin (SM),rifampicin (RFP) and isoniazid (INH) genotypes.It possesses high clinical value.

Adult ; Aged ; Contracture ; congenital ; genetics ; Fingers ; abnormalities ; Hand Deformities, Congenital ; genetics ; Humans ; Male ; Middle Aged ; Pedigree

Adolescent ; Adult ; Asian Continental Ancestry Group ; genetics ; Child ; China ; Deafness ; genetics ; Female ; Humans ; Male ; Pedigree ; Young Adult

Purpose To study the expression of TMPRSS2, ERG and ETV1 in prostatic cancer and their clinical pathologic signifi-cance. Methods Tissue microarray and immunohistochemistry (MaxVision) were used to detect TMPRSS2, ERG and ETV1 expres-sion in 70 prostatic cancer tissues, 10 prostatic intraepithelial neoplasia tissues and 18 benign prostate tissues. Results There was no statistical significance on positive rate of the expression of TMPRSS2 among prostatic cancer tissues, prostatic intraepithelial neoplasia tissues and benign prostate tissues (P>0. 05). The positive rate (81. 4%) of ERG in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues ( 30. 0%) and benign prostate tissues ( 0. 0 ) ( P <0. 05 ) . The positive rate (68. 6%) of ETV1 in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues (50. 0%) and benign prostate tissues (22. 2%) (P<0. 05). There was no correlation among the positive rates of TMPRSS2, ERG and ETV1 in prostatic cancer tissues and age (P>0. 05). The expression of TMPRSS2, ERG and ETV1 was positively correlated to Gleason score and clinical stage (P<0. 05). TMPRSS2 had positive correlation with ERG (rs =0. 465, P<0. 01). TMPRSS2 had positive correla-tion with ETV1 (rs =0. 590, P<0. 01). ERG had no positive correlation with ETV1 (rs =0. 151, P>0. 05). Conclusion ERG and ETV1 are expected to become therapeutic targets for prostate cancer. Detecting TMPRSS2, ERG and ETV1 at the same time is helpful to diagnosis and differential diagnosis of prostatic cancer, which might be new molecule markers of prostate cancer.

Objective To identify the candidate genes in the vicinity of a susceptibility locus (urinary albumin 1,UA-1) contributing to the development of albuminuria in type 2 diabetic KK/Ta mice. Methods Total RNA was extracted from the kidneys of KK/Ta (n=3) and BALB/c (n=2) mice at 20 weeks of age.The gene expression profile in kidney was investigated using the Affymetrix Murine Genome U74Av2 array.Competitive RT-PCR was used to confirm the differential expression of syndecan-4 which located in the vicinity of UA-1.Genome DNA was extracted from KK/Ta and BALB/c mice.DNA sequence analysis of the coding and promotor region of syndecan-4 gene was conducted. Results In the vicinity of the susceptibility locus (UA-1)contributing to the development of albuminuria in type 2 diabetic KK/Ta mice,10 candidate genes that showed differential expression were identified.Among them,the gene expression of syndecan-4in KK/Ta kidneys at 20 weeks of age was up-regulated by 26.1 times of age-matched BALB/c kidneys.Sequence analysis revealed two synonymous polymorphisms in the coding region (A93C and T216C) and three polymorphisms in the promoter region (-T263C,-T396C and -G669A) of the syndecan-4 gene.The TATA box was found at 321 bp upstream from the transcription start site,and the T263C polymorphism was located in the binding site of transcription factor Clox.Conclusions Syndecan-4 gene is mapped in the vicinity of the susceptibility locus contributing to the development of albuminuria in type 2 diabetes.The gene expression of syndecan-4 in KK/Ta kidneys is up-regulated than that in age-matched BALB/c kidneys at 20 weeks of age.Thus syndecan-4 may be one of the potential candidate genes responsible for diabetic nephropathy.Sequence differences in the promoter region influence the expression levels of syndecan-4 genes in KK/Ta kidneys.

OBJECTIVEThe molecular targets of ginsenoside Rg1-induced neural stem cells (NSCs) differentiation were screened by genechip.

METHOD7th day following ginsenoside Rg1 induced human neural stem cells to neurons the gene expression was observed by genechip. The purpose gene and signal transduction pathways were selected by the data calculations, and then confirmed by western blot and immunohistochemical method.

RESULT7th day following Rg1-induced NSCs differentiation, there were about 675 different genes, 255 genes of which were up-regulated and 420 genes down-regulated obviously. Meanwhile the ERK1/2 (extracellular signal-regulated protein kinase) in MAPK (mitogen-activated protein kinase) pathway was related with the NSCs differentiation. The Western blot and immunohistochemistry detection confirmed that ERK 1/2 protein and its phosphorylation were significantly increased, which can be blocked by PD98059 (ERK1/2 inhibitor). In addition, differentiation rate of NSCs was also decreased obviously in ginsenoside Rg1-induced differentiated NSCs when ERK blocker PD98059 was used.

CONCLUSIONERK1/2 is an important molecular target in ginsenoside Rg1-induced NSC differentiation. The selected differentially expressed genes by genechip may provide new clues to study of ginsenoside Rg1-induced NSCs differentiation.

Cell Differentiation ; drug effects ; Cell Line ; Down-Regulation ; drug effects ; Flavonoids ; pharmacology ; Ginsenosides ; pharmacology ; Humans ; MAP Kinase Signaling System ; drug effects ; genetics ; Neural Stem Cells ; cytology ; drug effects ; metabolism ; Oligonucleotide Array Sequence Analysis ; Protein Kinase Inhibitors ; pharmacology ; Time Factors