Purpose: Developmentally regulated GTP-binding protein 2 (DRG2) regulates microtubule dynamics and G2/M arrest during docetaxel treatment. Poly ADP-ribose polymerase (PARP) acts as an important repair system for DNA damage caused by docetaxel treatment. This study investigated whether DRG2 expression affects response to PARP inhibitors (olaparib) using prostate cancer cell lines PC3, DU145, LNCaP-FGC, and LNCaP-LN3. Materials and Methods: The cell viability and DRG2 expression levels were assessed using colorimetric-based cell viability assay and western blot. Cells were transfected with DRG2 siRNA, and pcDNA6/V5-DRG2 was used to overexpress DRG2. Flow cytometry was applied for cell cycle assay and apoptosis analysis using the Annexing V cell death assay. Results: The expression of DRG2 was highest in LNCaP-LN3 and lowest in DU145 cells. Expressions of p53 in PC3, DU145, and the two LNCaP cell lines were null-type, high-expression, and medium-expression, respectively. In PC3 (DRG2 high, p53 null) cells, docetaxel increased G2/M arrest without apoptosis; however, subsequent treatment with olaparib promoted apoptosis. In DU145 and LNCaP-FGC (DRG2 low), docetaxel increased sub-G1 but not G2/M arrest and induced apoptosis, whereas olaparib had no additional effect. In LNCaP-LN3 (DRG2 high, p53 wild-type), docetaxel increased sub-G1 and G2/M arrest, furthermore olaparib enhanced cell death. Docetaxel and olaparib combination treatment had a slight effect on DRG2 knockdown PC3, but increased apoptosis in DRG2-overexpressed DU145 cells. Conclusions DRG2 and p53 expressions play an important role in prostate cancer cell lines treated with docetaxel, and DRG2 levels can predict the response to PARP inhibitors.

The anticancer drugs have evolved significantly, spanning molecular targeted therapeutics (MTTs), immune checkpoint inhibitors (ICIs), chimeric antigen receptor T-cell (CAR-T) therapy, and antibody-drug conjugates (ADCs). Complications associated with these drugs vary widely based on their mechanisms of action. MTTs that target angiogenesis can often lead to complications related to ischemia or endothelial damage across various organs, whereas non-anti-angiogenic MTTs present unique complications derived from their specific pharmacological actions. ICIs are predominantly associated with immunerelated adverse events, such as pneumonitis, colitis, hepatitis, thyroid disorders, hypophysitis, and sarcoid-like reactions. CAR-T therapy causes unique and severe complications including cytokine release syndrome and immune effector cell-associated neurotoxicity syndrome. ADCs tend to cause complications associated with cytotoxic payloads. A comprehensive understanding of these drug-specific toxicities, particularly using medical imaging, is essential for providing optimal patient care. Based on this knowledge, radiologists can play a pivotal role in multidisciplinary teams. Therefore, radiologists must stay up-to-date on the imaging characteristics of these complications and the mechanisms underlying novel anticancer drugs.

Excessive wear of the teeth, which causes functional, aesthetic problems, or sensitivity symptoms, is referred to as pathological wear. If this leads to the collapse of occlusion, extensive restoration is necessary to restore function and aesthetics.When restoring pathologically worn dentition, it is essential to identify the cause of the wear before determining treatment plan to get a favorable prognosis. In this case, a patient exhibited severe tooth wear with decreased vertical dimension and clinical, radiographic examination, and medical history indicated the pathological wear caused by a combination of factors. Consequently, full-mouth rehabilitation was indicated and the vertical occlusal height was determined considering the appropriate restorative space. Based on the causes of the wear, the restoration method, materials, and occlusal pattern were selected and successfully restored to normal occlusal state through full-mouth rehabilitation with zirconia full-veneered crowns.

Purpose: . The purpose of this study is to analyze the biomechanical differences in rotation angle-torque according to the type of implant and to compare the effects of contamination and sandblasting on the removal torque value (RTV) of abutment screws. Materials and methods: . For this study, an automated device for tightening and loosening implant screws was developed, and each 15 implant-abutment complexes of external and internal connection type were prepared, divided into three groups according to the surface treatment of the screw:control group (no treatment), experimental group-1 (artificial saliva contamination and chlorhexidine (CHX) rinsing), and experimental group-2 (artificial saliva contamination, CHX rinsing, and subsequent 50 µm Al 2 O 3 sandblasting). FirstRTV was measured for each group, followed by different post-treatment procedures for the screws, and then Second-RTV was measured. During the procedure, the removal torque value and rotation angle vs. time were recorded at a 20 data/s.A standardized protocol was followed for all the tightening and loosening procedures: screw was tightened with 30 Ncm torque and maintained for 5 s, and then rotated in the reverse direction until the torque value reached 0 Ncm. After 10min of rest period, the screws were loosened to initial point. Results: . Statistical analysis of measured data revealed that the internal connection type showed significantly higher First-RTV compared to the external connection type, however, no significant differences in Second-RTV were found. Both experimental groups 1and 2 showed a decrease in RTVs for both implant types, while the control group showed a decrease in RTV only for the internal type. The internal connection type required a significantly larger rotation angle during the tightening and loosening process. Conclusion . Within the limitations of this study, both artificial saliva contamination and CHX cleansing, as well as sandblasting, decreased RTV in both internal and external connection types. Internal connections were more susceptible to the effects of contaminants.

The occlusal collapse resulting from loss of posterior tooth support and attrition necessitates treatment aimed at restoring normal function and aesthetics.Particularly in cases where there is insufficient space for restoration, full mouth rehabilitation accompanied by increased vertical dimension is necessary. This case report presents a complete mouth rehabilitation of a patient with inadequate anterior restoration space due to loss of posterior tooth support, utilizing guided implant surgery, facial scan, virtual articulator, and jaw motion tracker.

Reliable preclinical models for assessing drug-induced cardiotoxicity are essential to reduce the high rate of drug withdrawals during development. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a promising platform for such assessments due to their expression of cardiacspecific ion channels and electrophysiological properties. In this study, we investigated the effects of eight arrhythmogenic drugs—E4031, nifedipine, mexiletine, JNJ303, flecainide, moxifloxacin, quinidine, and ranolazine—on hiPSC-CMs derived from both healthy individuals and a long QT syndrome (LQTS) patient using multielectrode array systems. The results demonstrated dose-dependent changes in field potential duration and arrhythmogenic risk, with LQTS-derived hiPSC-CMs showing increased sensitivity to hERG channel blockers such as E4031. Furthermore, the study highlights the potential of hiPSC-CMs to model disease-specific cardiac responses, providing insights into genetic predispositions and personalized drug responses.Despite challenges related to the immaturity of hiPSC-CMs, their ability to recapitulate human cardiac electrophysiology makes them a valuable tool for preclinical cardiotoxicity assessments. This study underscores the utility of integrating patientderived hiPSC-CMs with advanced analytical platforms, such as multi-electrode array systems, to evaluate drug-induced electrophysiological changes. These findings reinforce the role of hiPSC-CMs in drug development, facilitating safer and more efficient screening methods while supporting precision medicine applications.

The anticancer drugs have evolved significantly, spanning molecular targeted therapeutics (MTTs), immune checkpoint inhibitors (ICIs), chimeric antigen receptor T-cell (CAR-T) therapy, and antibody-drug conjugates (ADCs). Complications associated with these drugs vary widely based on their mechanisms of action. MTTs that target angiogenesis can often lead to complications related to ischemia or endothelial damage across various organs, whereas non-anti-angiogenic MTTs present unique complications derived from their specific pharmacological actions. ICIs are predominantly associated with immunerelated adverse events, such as pneumonitis, colitis, hepatitis, thyroid disorders, hypophysitis, and sarcoid-like reactions. CAR-T therapy causes unique and severe complications including cytokine release syndrome and immune effector cell-associated neurotoxicity syndrome. ADCs tend to cause complications associated with cytotoxic payloads. A comprehensive understanding of these drug-specific toxicities, particularly using medical imaging, is essential for providing optimal patient care. Based on this knowledge, radiologists can play a pivotal role in multidisciplinary teams. Therefore, radiologists must stay up-to-date on the imaging characteristics of these complications and the mechanisms underlying novel anticancer drugs.

Excessive wear of the teeth, which causes functional, aesthetic problems, or sensitivity symptoms, is referred to as pathological wear. If this leads to the collapse of occlusion, extensive restoration is necessary to restore function and aesthetics.When restoring pathologically worn dentition, it is essential to identify the cause of the wear before determining treatment plan to get a favorable prognosis. In this case, a patient exhibited severe tooth wear with decreased vertical dimension and clinical, radiographic examination, and medical history indicated the pathological wear caused by a combination of factors. Consequently, full-mouth rehabilitation was indicated and the vertical occlusal height was determined considering the appropriate restorative space. Based on the causes of the wear, the restoration method, materials, and occlusal pattern were selected and successfully restored to normal occlusal state through full-mouth rehabilitation with zirconia full-veneered crowns.

Purpose: . The purpose of this study is to analyze the biomechanical differences in rotation angle-torque according to the type of implant and to compare the effects of contamination and sandblasting on the removal torque value (RTV) of abutment screws. Materials and methods: . For this study, an automated device for tightening and loosening implant screws was developed, and each 15 implant-abutment complexes of external and internal connection type were prepared, divided into three groups according to the surface treatment of the screw:control group (no treatment), experimental group-1 (artificial saliva contamination and chlorhexidine (CHX) rinsing), and experimental group-2 (artificial saliva contamination, CHX rinsing, and subsequent 50 µm Al 2 O 3 sandblasting). FirstRTV was measured for each group, followed by different post-treatment procedures for the screws, and then Second-RTV was measured. During the procedure, the removal torque value and rotation angle vs. time were recorded at a 20 data/s.A standardized protocol was followed for all the tightening and loosening procedures: screw was tightened with 30 Ncm torque and maintained for 5 s, and then rotated in the reverse direction until the torque value reached 0 Ncm. After 10min of rest period, the screws were loosened to initial point. Results: . Statistical analysis of measured data revealed that the internal connection type showed significantly higher First-RTV compared to the external connection type, however, no significant differences in Second-RTV were found. Both experimental groups 1and 2 showed a decrease in RTVs for both implant types, while the control group showed a decrease in RTV only for the internal type. The internal connection type required a significantly larger rotation angle during the tightening and loosening process. Conclusion . Within the limitations of this study, both artificial saliva contamination and CHX cleansing, as well as sandblasting, decreased RTV in both internal and external connection types. Internal connections were more susceptible to the effects of contaminants.

The occlusal collapse resulting from loss of posterior tooth support and attrition necessitates treatment aimed at restoring normal function and aesthetics.Particularly in cases where there is insufficient space for restoration, full mouth rehabilitation accompanied by increased vertical dimension is necessary. This case report presents a complete mouth rehabilitation of a patient with inadequate anterior restoration space due to loss of posterior tooth support, utilizing guided implant surgery, facial scan, virtual articulator, and jaw motion tracker.