Professor
Acupuncture ; Acupuncture Therapy ; Angina, Stable ; Combined Modality Therapy ; Humans ; Moxibustion

Objective To assess the effects of hypoxia combined with heavy physical workload on cardiac function of the military personnel who stationed at high altitude and to conclude the recovery effects as the personnel returning to normal altitude. Methods Ninety-six male military personnel,who rushed to plateau (3700 m) and undertaken heavy physical work for 50 days,were divided into severe,moderate, mild and no acute high altitude reaction (AHAR) group by their AHAR symptoms.Serum creatine kinase isoenzyme-MB (CK-MB) and lactic dehydrogenase isoenzyme-1(LDH-1),myocardial performance index (Tei index),left ventricular ejection fraction (LVEF),and left ventricular fractional shortening (LVFS) were respectively measured before they returned to,12 h and 15 d after they stationed at normal altitude (1500 m).Another 50 healthy volunteers who stationed at 1500 m were chosen as control group. Results The incidence of AHAR was 74.0％(71/96),including 32.5％ (25/71) mild,31.0％ (22/71) moderate and 33.8％ (24/71) severe cases.Level of serum CK-MB,LDH-1 and Tei index of severe group were significantly higher than those of moderate,mild and no AHAR group (P＜0.01),while LVEF,LVFS were significantly lower (P＜0.01).For stationed at 3700 m for 50 d,personnel's CK-MB and LDH-1 level was positively correlated with Tei index (r=0.625,0.598,P＜0.01),and negatively correlated with LVEF and LVFS (r=-0.716,-0.658,-0.639,-0.727,P＜0.01).The CK-MB and LDH-1 level and Tei index that gathered for 50-d stay were significantly higher than those of theirs stationed at 1500 m for 12 h and 15 d respectively,as well as higher than those of control group,but LVEF and LVFS were significantly lower in same comparison (t =7.146-28.613,P＜ 0.01).The self comparisons between 12 h and 15 d stationings respectively in AHAR and control group showed significant differences (t=8.677-17.852,P＜0.01).For staying 15 d at 1500 m,parameters showed no significant difference with control group's (t=0.612-1.558,P＞0.05). Conclnsions Hypoxia with heavy physical workload could obviously cause military personnel's cardiac function impairment and the harm is aggravated with the severity of AHAR.The cardiac function would obviously improve as returning to normal altitude for 12 h,and 15-d recovery would enable cardiac function normalized.

Objective To assess the effects of hypoxia combined with heavy physical workload on cardiac function of the military personnel who stationed at high altitude and to conclude the recovery effects as the personnel returning to normal altitude. Methods Ninety-six male military personnel,who rushed to plateau (3700 m) and undertaken heavy physical work for 50 days,were divided into severe,moderate, mild and no acute high altitude reaction (AHAR) group by their AHAR symptoms.Serum creatine kinase isoenzyme-MB (CK-MB) and lactic dehydrogenase isoenzyme-1(LDH-1),myocardial performance index (Tei index),left ventricular ejection fraction (LVEF),and left ventricular fractional shortening (LVFS) were respectively measured before they returned to,12 h and 15 d after they stationed at normal altitude (1500 m).Another 50 healthy volunteers who stationed at 1500 m were chosen as control group. Results The incidence of AHAR was 74.0％(71/96),including 32.5％ (25/71) mild,31.0％ (22/71) moderate and 33.8％ (24/71) severe cases.Level of serum CK-MB,LDH-1 and Tei index of severe group were significantly higher than those of moderate,mild and no AHAR group (P＜0.01),while LVEF,LVFS were significantly lower (P＜0.01).For stationed at 3700 m for 50 d,personnel's CK-MB and LDH-1 level was positively correlated with Tei index (r=0.625,0.598,P＜0.01),and negatively correlated with LVEF and LVFS (r=-0.716,-0.658,-0.639,-0.727,P＜0.01).The CK-MB and LDH-1 level and Tei index that gathered for 50-d stay were significantly higher than those of theirs stationed at 1500 m for 12 h and 15 d respectively,as well as higher than those of control group,but LVEF and LVFS were significantly lower in same comparison (t =7.146-28.613,P＜ 0.01).The self comparisons between 12 h and 15 d stationings respectively in AHAR and control group showed significant differences (t=8.677-17.852,P＜0.01).For staying 15 d at 1500 m,parameters showed no significant difference with control group's (t=0.612-1.558,P＞0.05). Conclnsions Hypoxia with heavy physical workload could obviously cause military personnel's cardiac function impairment and the harm is aggravated with the severity of AHAR.The cardiac function would obviously improve as returning to normal altitude for 12 h,and 15-d recovery would enable cardiac function normalized.

OBJECTIVEAn HPLC method was developed to determinate Ibuprofen and Pseudoephedrine Hydrochloride and Chlorpheniramine Maleate in Compound BuluoWeimaNamin Tablets.

METHODSUsing HPLC with Kromasil C18 column, and acetonitrile -0.5% SDS- phosphate (580:420:1) as the mobile phase. The wavelength for detection was 262 nm.

RESULTSBetter linearities and good correlation coefficients were obtained: the concentration ranges of ibuprofen, pseudoephedrine hydrochloride and chlorpheniramine maleate were over 2.062-14.434 microg (r=0.9999), 0.296-2.072l microg (r=0.9999), and 0.0204~0.1428 microg (r=0.9998), respectively. The recoveries of ibuprofen, pseudoephedrine hydrochloride and chlorpheniramine maleate were 99.98% (RSD=0.52%), 99.72 (RSD=0.82%) and 99.545 (RSD=0.76%), respectively.

CONCLUSIONThe method was convenient, accurate and specific. It can be used as a method to control quality of Compound Buluoweimanamin Tablets.

Chlorpheniramine ; analysis ; Chromatography, High Pressure Liquid ; methods ; Ibuprofen ; analysis ; Pseudoephedrine ; analysis ; Tablets ; chemistry