Plant polyphenols have a wide range of pharmacological activities and application prospects. Liquid polyphenol preparations have special physical phases and complex chemical compositions, with problems such as poor stability and easy precipitation during production and marketing. Taking the multi-precipitation mechanism of plant polyphenol liquid preparations as an example,we discuss the chemistry and composition of the precipitation, how it forms, whether precipitationcan be controlled, and the interaction law of three precipitation approaches. An unstable mechanism model is proposed where hydrolyzed tannin hydrolysis and catechin non-enzymatic oxidative polymerization repeatedly induces associative colloid aggregation and precipitation. This study explains the complex physicochemical changes in polyphenol solutions and the microcosmic mechanism of instability in the induced system and proposes a steady state reconstruction of liquid polyphenol preparation consistent with the common law of precipitation and control. It has scientific significance for promoting the development and manufacture of high quality liquid polyphenol preparations.

Antibodies, Monoclonal, Humanized/therapeutic use* ; China ; Humans ; Psoriasis/drug therapy* ; Severity of Illness Index ; Treatment Outcome

Ellagic acid is ubiquitous in plants and is considered as a potential candidate for antioxidant and antineoplastic drugs. However, ellagic acid has poor solubility and precipitates easily even after initial solubilization. Improvement of its bioavailability has been a concern of pharmaceutical industry. It was found that storage in Sanlejiang oral liquid at low temperature keeps its stability. Ellagic acid is anomalous in a way that is easily soluble at low temperatures but precipitates at high temperatures. In order to reveal the mechanism of this phenomenon and develop precipitation prevention and control strategies, ellagic acid in Sanlejiang oral liquid was stored at high, medium and low temperatures for three months. The changes of composition and phase state of the whole system during storage were systematically tracked and studied by means of precipitation amount or morphology, HPLC chemical profile of supernatant versus precipitates, and comprehensive characterization of physical phase state. The results show that the amount of precipitation at low temperature is only 1/3 of that at normal room temperature. As the temperature rises, the sedimentation increases sharply. HPLC analyses of supernatant versus precipitates revealed that ellagic acid precipitation originated from two ways: chemical degradation and physical deposition. The chemical sedimentation is related to the hydrolysis of tannins under acidic condition, forming chebulagic acid and corilagin. Physical sedimentation is related to the decrease of the association degree and viscosity of polyphenol colloids when temperature rises. This study elucidated the stability mechanism of ellagic acid in liquid preparations of TCM, and provided the mechanistic basis for efficient utilization and solution prepartion of ellagic acid.

OBJECTIVETo detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1).

METHODSThe activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cytotoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program.

RESULTSArbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50%>100 μmol/L)and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5.5 ± 0.9), (3.4 ± 0.8), and (1.5 ± 0.2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively.

CONCLUSIONArbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.

Animals ; Antiviral Agents ; pharmacology ; Dogs ; Indoles ; pharmacology ; Influenza A Virus, H1N1 Subtype ; drug effects ; Madin Darby Canine Kidney Cells ; Virus Replication ; drug effects

Objective To investigate the effects of HP and PHP maneuver training on human centrifuge for the purpose of establishing a scientific training approach on centrifuge. Methods According to the maneuverability of high performance fighter aircrafts,a 3-day centrifuge training program was constituted.The highest training G level was 6.0,7.0,9.0 G respectively for 3 training days.On the 1st day,preprogrammed acceleration profiles were adopted.After relaxed G tolerance test,pilots who wore anti-G suit or compensatory suit underwent HP maneuver training.On the 2nd and 3rd day,trainings of HP breathing were carried out during 3.0 G exposures.The close-loop controlled trainings were conducted during 5.0,7.0,8.0 and 9.0 G runs where pilots were performing HP and PHP maneuver.Fifty-five pilots went through the centrifuge trainings.Changes of +Gz tolerance,HR,breathing rate (BR),and muscular force of legs (Flegs) during the training were observed and analyzed. Results Fifty-two of the 55 pilots (94.55％) completed the training of 8.0 G for 10 s with the elevated G tolerance ranging from 2.0 G to 3.0 G.Flegs and HR raised significantly with the increment of G level (F =111.95-1253.21,P＜ 0.01).BR was controlled between 20-26 times/min.The trainees reported that the close-loop controlled training was more comfortable and preferred than passive controlled training. Conclusions The HP and PHP maneuver training approach on centrifuge could enhance pilots anti-G capability greatly,and thus the aerial combat performance of pilots.

Objective To investigate the effects of HP and PHP maneuver training on human centrifuge for the purpose of establishing a scientific training approach on centrifuge. Methods According to the maneuverability of high performance fighter aircrafts,a 3-day centrifuge training program was constituted.The highest training G level was 6.0,7.0,9.0 G respectively for 3 training days.On the 1st day,preprogrammed acceleration profiles were adopted.After relaxed G tolerance test,pilots who wore anti-G suit or compensatory suit underwent HP maneuver training.On the 2nd and 3rd day,trainings of HP breathing were carried out during 3.0 G exposures.The close-loop controlled trainings were conducted during 5.0,7.0,8.0 and 9.0 G runs where pilots were performing HP and PHP maneuver.Fifty-five pilots went through the centrifuge trainings.Changes of +Gz tolerance,HR,breathing rate (BR),and muscular force of legs (Flegs) during the training were observed and analyzed. Results Fifty-two of the 55 pilots (94.55％) completed the training of 8.0 G for 10 s with the elevated G tolerance ranging from 2.0 G to 3.0 G.Flegs and HR raised significantly with the increment of G level (F =111.95-1253.21,P＜ 0.01).BR was controlled between 20-26 times/min.The trainees reported that the close-loop controlled training was more comfortable and preferred than passive controlled training. Conclusions The HP and PHP maneuver training approach on centrifuge could enhance pilots anti-G capability greatly,and thus the aerial combat performance of pilots.

OBJECTIVETo study the efficacy, safety and reliability of colonic sac duct for first-stage repair of colorectal anastomotic leakage.

METHODSAn animal model of colon anastomotic leakage was established in 30 Tibet miniature pigs, which were randomly divided into treatment group and control group (n=15). Colon anastomotic leakage in the treatment group was repaired using the colonic sac duct, while the control group received conventional surgical repair. At 7, 14, and 21 days after the surgery, the healing of the anastomotic leakage was evaluated by examining the bursting pressure, tissue microvessel density and hydroxyproline content at the anastomosis.

RESULTSUsing the colonic sac duct, the anastomotic leakage was successfully repaired without death of the pigs or the occurrence of intestinal stenosis or necrosis. At 7 and 14 days after the surgery, the bursting pressure, hydroxyproline contents, and microvessel density in the treatment groups were higher than those in the control group, but such difference was not found at 21 days.

CONCLUSIONColonic sac duct allows effective repair of colon anastomotic leakage, and is especially useful for leakage lasting for 48-72 h complicated by severe abdominal infection.

Anastomosis, Surgical ; adverse effects ; Anastomotic Leak ; etiology ; surgery ; Animals ; Colon ; surgery ; Female ; Male ; Rectum ; surgery ; Swine ; Swine, Miniature

In the two decades since AZT was first approved for clinical use in 1987, 24 additional antiretroviral agents have been approved. They include 7 nucleoside analogs, a nucleotide analog and 4 non-nucleoside reverse transcriptase inhibitors, 10 protease inhibitors, 2 entry inhibitors and an integrase inhibitor. More than 20 investigational agents are currently being studied in clinical trials. Highly active antiretroviral therapy (HAART), which involves a combination of anti-HIV-1 drugs, is extremely effective in suppressing HIV-1 replication and increasing CD4+ number and results in substantial reductions in HIV-1-related morbidity and mortality. In last 20 years, much has been learned about resistance to antiretroviral drugs, drug interactions and metabolic complications of antiviral drug use. Drugs are now selected on the basis of resistance tests and on the risk of specific drug complications in individual patients. As a result, decisions about the therapy of HIV/AIDS have become personalized and are made on a patient-by-patient basis. With appropriate medical management, a person with HIV-1 now has the possibility of a nearly normal life expectancy.
Anti-HIV Agents ; adverse effects ; pharmacology ; therapeutic use ; Antiretroviral Therapy, Highly Active ; Cyclohexanes ; chemistry ; pharmacology ; therapeutic use ; Drug Resistance, Viral ; HIV Envelope Protein gp41 ; chemistry ; therapeutic use ; HIV Fusion Inhibitors ; chemistry ; pharmacology ; therapeutic use ; HIV Infections ; drug therapy ; HIV Integrase Inhibitors ; chemistry ; pharmacology ; therapeutic use ; HIV Protease Inhibitors ; chemistry ; pharmacology ; therapeutic use ; HIV Reverse Transcriptase ; chemistry ; pharmacology ; therapeutic use ; HIV-1 ; drug effects ; physiology ; Humans ; Molecular Structure ; Peptide Fragments ; chemistry ; therapeutic use ; Pyrrolidinones ; chemistry ; pharmacology ; therapeutic use ; Raltegravir Potassium ; Saquinavir ; chemistry ; pharmacology ; therapeutic use ; Triazoles ; chemistry ; pharmacology ; therapeutic use ; Virus Replication ; drug effects ; Zidovudine ; chemistry ; pharmacology ; therapeutic use

Objective To observe the changes of antithrombin activity(AT) and D-dimer in acute leukemia(AL)children complicated with disseminated intravascular coagulation(DIC) and to explore the changes of blood coagulation and fibrinolysis function.Methods Twenty-seven AL children without DIC were selected as AL group and 25 childern complicated with DIC were selected as observe group,36 health children were as control group.Plasma level of AT,D-dimer,fibrinogen,activated partial thromboplastin time and prothrombin time were tested by color substrate,immuno-latex turbidimetry,and coagulation method.And the rusults of AL group were compared with observe group and control group by SPSS 10.0 software.Results PT was significantly prolonged and the D-dimer in AL group and observation group were significantly higher than those in control group(Pa