AIM:To explore the mechanism of Periplaneta americana extracts(PA-CⅡ-3)on anti-prostate cancer.METHODS:Prostate cancer cells PC3 in logarithmic growth phase were divided into a control group and an experimental group.The PA-CⅡ-3 solutions of 1 000,1 200,1 400,1 600,1 800,and 2 000 μg/mL were added successively to the PC3 cells in experimental group,for screening out the appropriate drug concentration and best time to intervene by CCK-8 assay.And then,PA-CⅡ-3 so-lution at 1 400 μg/mL was continuously intervened in PC3 cells for 72 hours.The cell proliferation abili-ty was detected by CCK-8 assay and plate colony formation assay,the cell migration ability was test-ed by scratch wound assay,and the mRNA and pro-tein expressions of ZAG were detected by Real time qPCR and Western blot methods,respectively.RE-SULTS:After PA-CⅡ-3 intervention,the proliferation and migration abilities of PC3 cells were significant-ly inhibited,and the mRNA and protein expression levels of ZAG in PC3 cells were promoted.CONCLU-SION:PA-CⅡ-3 can play the role of anti-prostate cancer by inhibiting the proliferation and migration of PC3 cells,and upregulating ZAG.

AIM:To explore the mechanism of Periplaneta americana extracts(PA-CⅡ-3)on anti-prostate cancer.METHODS:Prostate cancer cells PC3 in logarithmic growth phase were divided into a control group and an experimental group.The PA-CⅡ-3 solutions of 1 000,1 200,1 400,1 600,1 800,and 2 000 μg/mL were added successively to the PC3 cells in experimental group,for screening out the appropriate drug concentration and best time to intervene by CCK-8 assay.And then,PA-CⅡ-3 so-lution at 1 400 μg/mL was continuously intervened in PC3 cells for 72 hours.The cell proliferation abili-ty was detected by CCK-8 assay and plate colony formation assay,the cell migration ability was test-ed by scratch wound assay,and the mRNA and pro-tein expressions of ZAG were detected by Real time qPCR and Western blot methods,respectively.RE-SULTS:After PA-CⅡ-3 intervention,the proliferation and migration abilities of PC3 cells were significant-ly inhibited,and the mRNA and protein expression levels of ZAG in PC3 cells were promoted.CONCLU-SION:PA-CⅡ-3 can play the role of anti-prostate cancer by inhibiting the proliferation and migration of PC3 cells,and upregulating ZAG.

BACKGROUND AND PURPOSE: The objective of this study was to determine the prevalence of and risk factors for dementia in residents aged 65 years and older in a Chinese nursing home. METHODS: A cross-sectional study was carried out in a nursing home located in Hangzhou, Zhejiang Province, China. Cognitive status, including the presence of dementia and mild cognitive impairment (MCI), was measured using a combination of medical history and objective cognitive assessments. Logistic regression analysis was conducted to predict the associated risk factors. RESULTS: In total, 943 residents (334 males and 609 females) aged 84.00±6.67 years (mean±SD) were included. Dementia was diagnosed in 420 (44.5%) residents, and MCI was diagnosed in 195 (20.7%). Mild, moderate, and severe dementia were present in 20.3%, 14.0%, and 65.7% of those in the dementia group, respectively. Logistic regression analysis revealed that dementia was associated with a low education level (p=0.000), an advanced age (p=0.010), and a history of stroke (p=0.023). CONCLUSIONS: Our study found a high prevalence of dementia in a Chinese nursing home, and a high prevalence of patients with severe dementia. Risk factors for dementia included a low educational level, an advanced age, and a history of stroke. Appropriate interventions need to be applied to this population.
Asian Continental Ancestry Group* ; China ; Cross-Sectional Studies ; Dementia* ; Education ; Humans ; Logistic Models ; Male ; Mild Cognitive Impairment ; Nursing Homes* ; Nursing* ; Prevalence* ; Risk Factors* ; Stroke

Objective:To study the practical methods and the results of the rehabilitation education.Methods: One hundred cardiovascular patients over 65 years old who are receiving the com prehensive rehabilitation treatment are selected for rehabilitation education,an d comparisons were conducted between pre-and post-education.Results: After receiving the rehabilitation education,the cognition on disease (51%→89%)、rehabilitation therapy(47%→93%),confidence of to win a victory on d i sease(53%→90%)、attendance rate(71%→98%) increase obviously,and irrationality living habit decrease obviously(36%→13%). Conclusion: The rehabilitation education plays an important role in the rehabilitation of t he aged cardiovascular patients,and it is very practical and feasible.