Objective:To analyze the current status, hotspots, and future research trends of Ganmai Dazao Decoction.Methods:Literature about Ganmai Dazao Decoction was retrieved from the CNKI, Wanfang Data, Chongqing VIP, and CBM from January 1, 1994, to June 30, 2024. CiteSpace 6.3.R1 software was used for co-occurrence analysis of authors and institutions, as well as co-occurrence, clustering, timeline, and burst analysis of keywords.Results:A total of 696 articles were included, with an overall fluctuating upward trend in annual publications in this field, peaking in 2018 (51 articles). The institution with the most publications was Nanjing University of Chinese Medicine (12 articles); the journal with the most publications was Inner Mongolia Journal of Traditional Chinese Medicine (28 articles); among the authors, five published the most, with only 3 articles each. Research mainly focused on clinical applications of TCM, theory exploration, animal experiments, and other areas. Conclusions:The publication volume of Ganmai Dazao Decoction research shows an overall upward trend, with high attention on the treatment of diseases such as depression, organ dysregulation, and perimenopausal syndrome. The research hotspots include the mechanism of Ganmai Dazao Decoction in anti-depression and endocrine regulation and so on.

Objective·To investigate the effects and molecular mechanisms of phosphatidylethanolamine(PE)on macrophage senescence and its senescence-associated secretory phenotype(SASP),as well as its pathophysiological role in liver injury.Methods·A macrophage senescence model was established using doxorubicin(DOX),followed by PE treatment.A mouse liver injury model was generated via intraperitoneal co-administration of PE and lipopolysaccharide(LPS)to investigate the effects of PE on liver injury.Senescence markers and SASP factors,including senescence-associated β-galactosidase(SA-β-gal),cell cycle inhibitor p21,tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6),were evaluated using SA-β-gal staining,quantitative real-time PCR,and Western blotting.RNA sequencing(RNA-seq)was performed,followed by Gene Ontology(GO)cellular component enrichment analysis,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,Gene Set Variation Analysis(GSVA),and Gene Set Enrichment Analysis(GSEA),to explore the molecular mechanisms and signaling pathways by which PE promotes macrophage senescence.The expression of endoplasmic reticulum(ER)stress-related proteins,including inositol-requiring enzyme 1 α(IRE1α),spliced X-box binding protein 1(XBP1s),activating transcription factor 6(ATF6),ATF4,and C/EBP homologous protein(CHOP),was analyzed through in vivo and in vitro experiments.Results·PE significantly promoted the expression of senescence markers SA-β-gal,p21,p16 and SASP factors.RNA-seq analysis revealed that ER stress was involved in PE-induced promotion of SASP.Further experiments demonstrated that PE activated the ER stress signaling pathway,promoting macrophage senescence and the expression of SASP factors.In vivo experiments further confirmed that PE exacerbated LPS-induced liver injury in mice through ER stress.Conclusion·PE promotes macrophage senescence and the expression of SASP factors by activating ER stress signaling pathway,thereby aggravating LPS-induced liver injury.

Objective:To evaluate the association between heatwaves and fall-related mortality.Methods:A total of 61 421 fall-related mortality from 2013 to 2022 in 7 provinces of China were included in a time-stratified case-crossover design, with daily meteorological data derived from the fifth generation European Reanalysis dataset produced by the European Centre for Medium-Range Weather Forecasts. Conditional logistic regression chimeric distributed lag nonlinear model was used to analyze the association between heatwaves and fall-related mortality and stratified analysis was conducted according to gender and age.Results:Heatwaves were associated with an increased risk of fall-related morality. The risk of fall-related mortality during heatwaves was higher than during non-heatwave periods ( OR=1.11, 95% CI: 1.05-1.18). The attributable fraction of fall-related motality due to heatwaves was 10.25% (95% CI: 4.49%-15.36%). For each 1 ℃ increase above the heatwave threshold, the risk of fall-related mortality increased by 34% ( OR=1.34, 95% CI: 1.02-1.76). The effect of heatwave duration on fall-related mortality was not statistically significant. Stratified analyses indicated that women experienced a higher risk of fall-related mortality during heatwaves ( OR=1.13, 95% CI: 1.04-1.22) compared to man ( OR=1.10, 95% CI: 1.04-1.17). Conclusions:Heatwave increases the risk of fall-related mortality, and the intensity of heatwaves modify this risk. Women are vulnerable populations.

Objective:To evaluate the association between heatwaves and fall-related mortality.Methods:A total of 61 421 fall-related mortality from 2013 to 2022 in 7 provinces of China were included in a time-stratified case-crossover design, with daily meteorological data derived from the fifth generation European Reanalysis dataset produced by the European Centre for Medium-Range Weather Forecasts. Conditional logistic regression chimeric distributed lag nonlinear model was used to analyze the association between heatwaves and fall-related mortality and stratified analysis was conducted according to gender and age.Results:Heatwaves were associated with an increased risk of fall-related morality. The risk of fall-related mortality during heatwaves was higher than during non-heatwave periods ( OR=1.11, 95% CI: 1.05-1.18). The attributable fraction of fall-related motality due to heatwaves was 10.25% (95% CI: 4.49%-15.36%). For each 1 ℃ increase above the heatwave threshold, the risk of fall-related mortality increased by 34% ( OR=1.34, 95% CI: 1.02-1.76). The effect of heatwave duration on fall-related mortality was not statistically significant. Stratified analyses indicated that women experienced a higher risk of fall-related mortality during heatwaves ( OR=1.13, 95% CI: 1.04-1.22) compared to man ( OR=1.10, 95% CI: 1.04-1.17). Conclusions:Heatwave increases the risk of fall-related mortality, and the intensity of heatwaves modify this risk. Women are vulnerable populations.

Objective·To investigate the effects and molecular mechanisms of phosphatidylethanolamine(PE)on macrophage senescence and its senescence-associated secretory phenotype(SASP),as well as its pathophysiological role in liver injury.Methods·A macrophage senescence model was established using doxorubicin(DOX),followed by PE treatment.A mouse liver injury model was generated via intraperitoneal co-administration of PE and lipopolysaccharide(LPS)to investigate the effects of PE on liver injury.Senescence markers and SASP factors,including senescence-associated β-galactosidase(SA-β-gal),cell cycle inhibitor p21,tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6),were evaluated using SA-β-gal staining,quantitative real-time PCR,and Western blotting.RNA sequencing(RNA-seq)was performed,followed by Gene Ontology(GO)cellular component enrichment analysis,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,Gene Set Variation Analysis(GSVA),and Gene Set Enrichment Analysis(GSEA),to explore the molecular mechanisms and signaling pathways by which PE promotes macrophage senescence.The expression of endoplasmic reticulum(ER)stress-related proteins,including inositol-requiring enzyme 1 α(IRE1α),spliced X-box binding protein 1(XBP1s),activating transcription factor 6(ATF6),ATF4,and C/EBP homologous protein(CHOP),was analyzed through in vivo and in vitro experiments.Results·PE significantly promoted the expression of senescence markers SA-β-gal,p21,p16 and SASP factors.RNA-seq analysis revealed that ER stress was involved in PE-induced promotion of SASP.Further experiments demonstrated that PE activated the ER stress signaling pathway,promoting macrophage senescence and the expression of SASP factors.In vivo experiments further confirmed that PE exacerbated LPS-induced liver injury in mice through ER stress.Conclusion·PE promotes macrophage senescence and the expression of SASP factors by activating ER stress signaling pathway,thereby aggravating LPS-induced liver injury.

Objective: To study the correlation between electronic screen use and myopia among primary and secondary school students in six provinces and cities in China, in order to provide a scientific basis for comprehensive prevention and control of myopia. Methods: From November 2020 to June 2022, a total of 16 557 primary and secondary school students from six provinces and cities in China (Beijing City, Liaoning Province, Zhejiang Province, Henan Province, Shaanxi Province, Chongqing City) were selected by stratified cluster random sampling and probability smampling methods, and a questionnaire prepared by Beijing Center for Disease Control and Prevention was used to investigate their electronic screen use. According to Screening Criteria for Myopia in Children and Adolescents, 0.5% mass concentration of compound topicamide eye drops was used to paralyze the ciliary muscle and undergo slit lamp optometry. Chisquare test was used to compare the differences between groups, and binary Logistic regression was used to analyze the association between electronic screen use and myopia. Results: About 58.3% of primary and secondary school students used electronic screens for more than two hours a day on average, and 63.4% of primary and secondary school students used continuously electronic products for more than 15 minutes at a time for nonstudy purposes. After adjusting for confounding factors, parents unrestricted use of electronic screen time and electronic screen time ≥2 h/d were positively correlated with myopia (OR=1.27, 1.13, P<0.05). Gender stratified analysis showed that boys who used electronic screen time ≥2 h/d had a higher risk of myopia (OR=1.15, P<0.05). The results of grade stratification analysis showed that parents unrestricted electronic screen use time and electronic screen time ≥2 h/d were positively correlated with myopia in the lower grade of primary school students (OR=1.34, 1.18, P<0.05). Among the higher grade of primary school students, continuous use of electronic screens for nonstudy purposes for more than 15 minutes at one time was positively correlated with myopia (OR=1.18, P<0.05). There was a multiplicative interaction between total screen time and one continuous screen time (OR=1.04, P<0.05). Conclusions Primary and secondary students in six provinces and cities in China reports excessive electronic screen usage which is associated with myopia. Schools and parents should jointly limit the use of electronic screen among primary and secondary students to reduce the occurrence of myopia.

Objective:To investigate the research status and hotspots of Linggui Zhugan Decoction.Methods:The research literature about Linggui Zhugan Decoction was retrieved from CNKI, VIP, Wanfang Data and CBM databases from January 1, 2000 to July 11, 2023. NoteExpress 3.8 software was used to merge and remove the weight, and CiteSpace 6.2.R4 software was used to analyze the author, research institution, key words and draw the knowledge map.Results:A total of 1 521 articles were included, and the number of published papers showed a fluctuating upward trend. The main source journals were Chinese Journal of Urban and Rural Enterprise Hygiene, Acta Chinese Medicine, Information of Traditional Chinese Medicine, etc. The main research institutions were Tianjin University of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Shandong University of Traditional Chinese Medicine, etc. Among the most published papers were Huang Jinling from Anhui University of Chinese Medicine (22 articles), Wang Liang from the Institute of Integrated Chinese and Western Medicine of Anhui Academy of Chinese Medicine (14 articles) and Shi Hui from Anhui University of Chinese Medicine (10 articles); high-frequency keywords included chronic heart failure, vertigo, clinical efficacy and so on. Conclusions:The research on Linggui Zhugan Decoction focuses on the clinical research of circulatory system, respiratory system, digestive system and urinary system. The research focus has gradually developed from clinical study, prescription composition and efficacy study, network pharmacology study, mechanism and related theory exploration of prescriptions to clinical practical application.

Objective:To investigate the dynamic brain function changes in adolescent depression patients with or without suicidal ideation, and to explore the characteristics and the hypothalamus-pituitary-adrenal axis of adrenocorticotropic hormone (ACTH) and the potential correlation between levels of the hormone cortisol.Methods:From March 2018 to January 2021, the First Affiliated Hospital of Jinan University conducted a study involving 48 adolescent patients with major depressive disorder (MDD) who have suicidal ideation(MDD+SI), 38 MDD patients without suicidal ideation(MDD-SI), and 41 healthy control participants(HC). The study utilized resting state functional magnetic resonance imaging (fMRI) to analyze the brain activity of these individuals. The dynamic local consistency (dReHo) and dynamic functional connection (dFC) values for the three groups were computed utilizing a sliding time window analysis technique. Blood samples were obtained within 24 hours following the completion of the MRI scan to quantify ACTH and cortisol levels via direct chemiluminescence. A one-way analysis of covariance was employed to compare the dReHo and dFC values among the three groups, while a one-way analysis of variance was utilized to compare the hormone levels across the three groups. Significant differences in dReHo values among the three groups were identified in specific regions, as well as in dFC values, using the least significant differences method correction following inspection ( P<0.05). Additionally, a partial correlation analysis was conducted to investigate the relationship between these dynamic indexes and hormone levels. Results:The difference of dReHo values among the three groups was found in the right posterior cerebellum (mainly Crus Ⅱ and Crus I) ( F=9.44, P<0.001), and the left precuneus ( F=10.93, P<0.05). There were significant differences in dFC values between the three groups in the left precuneus and left middle temporal gyrus ( F=9.60, P<0.001), and the left precuneus with bilateral precuneus ( F=9.66, P<0.001). The correlation analysis revealed a positive relationship between the decreased dReHo ( r=0.350, P=0.018) variability in the left precuneus, the decreased dFC( r=0.362, P=0.015) variability between the left precuneus and the left middle temporal gyrus (MTG), and the decreased cortisol level( P=0.013, 0.039) in the group of MDD+SI. Conclusion:Adolescents diagnosed with MDD, both with and without suicidal ideation, exhibit dynamic functional alterations in the precuneus and middle temporal gyrus. The cerebellar function changes appear to be unique to those with suicidal ideation. Furthermore, the aberrant dynamic brain function observed in the precuneus and middle temporal gyrus is associated with abnormal cortisol levels in individuals with suicidal ideation.

Objective:To investigate the dynamic brain function changes in adolescent depression patients with or without suicidal ideation, and to explore the characteristics and the hypothalamus-pituitary-adrenal axis of adrenocorticotropic hormone (ACTH) and the potential correlation between levels of the hormone cortisol.Methods:From March 2018 to January 2021, the First Affiliated Hospital of Jinan University conducted a study involving 48 adolescent patients with major depressive disorder (MDD) who have suicidal ideation(MDD+SI), 38 MDD patients without suicidal ideation(MDD-SI), and 41 healthy control participants(HC). The study utilized resting state functional magnetic resonance imaging (fMRI) to analyze the brain activity of these individuals. The dynamic local consistency (dReHo) and dynamic functional connection (dFC) values for the three groups were computed utilizing a sliding time window analysis technique. Blood samples were obtained within 24 hours following the completion of the MRI scan to quantify ACTH and cortisol levels via direct chemiluminescence. A one-way analysis of covariance was employed to compare the dReHo and dFC values among the three groups, while a one-way analysis of variance was utilized to compare the hormone levels across the three groups. Significant differences in dReHo values among the three groups were identified in specific regions, as well as in dFC values, using the least significant differences method correction following inspection ( P<0.05). Additionally, a partial correlation analysis was conducted to investigate the relationship between these dynamic indexes and hormone levels. Results:The difference of dReHo values among the three groups was found in the right posterior cerebellum (mainly Crus Ⅱ and Crus I) ( F=9.44, P<0.001), and the left precuneus ( F=10.93, P<0.05). There were significant differences in dFC values between the three groups in the left precuneus and left middle temporal gyrus ( F=9.60, P<0.001), and the left precuneus with bilateral precuneus ( F=9.66, P<0.001). The correlation analysis revealed a positive relationship between the decreased dReHo ( r=0.350, P=0.018) variability in the left precuneus, the decreased dFC( r=0.362, P=0.015) variability between the left precuneus and the left middle temporal gyrus (MTG), and the decreased cortisol level( P=0.013, 0.039) in the group of MDD+SI. Conclusion:Adolescents diagnosed with MDD, both with and without suicidal ideation, exhibit dynamic functional alterations in the precuneus and middle temporal gyrus. The cerebellar function changes appear to be unique to those with suicidal ideation. Furthermore, the aberrant dynamic brain function observed in the precuneus and middle temporal gyrus is associated with abnormal cortisol levels in individuals with suicidal ideation.

Objective:To explore the teaching experience of clinical teaching teachers in master of integrated traditional Chinese and western medicine nursing, so as to provide a reference for improving the training quality of master of integrated Chinese and western medicine nursing.Methods:From March to May 2022, a total of 14 clinical teaching teachers of integrated traditional Chinese and western medicine nursing in the First Affiliated Hospital of Hunan University of were enrolled using the objective sampling method and investigated by the semi-structured face-to-face interview. Content analysis was used to analyze the interview results.Results:Five themes were extracted, namely, the practice ability of nursing graduate students needs to be improved, the evaluation methods of clinical practice ability of graduate students need to be more diversified, teaching benefits teachers as well as students; difficulties of teaching (conflicts between teachers' clinical work and teaching, unclear orientation of postgraduate clinical practice learning) , hope to get support (improve scientific research ability, improve theoretical knowledge of traditional Chinese medicine, guarantee the clinical practice and study time of graduate students) .Conclusions:A variety of practice approaches should be provided for the training in master of integrated traditional Chinese and western medicine nursing graduate students, to improve students' clinical practice ability and professional identity, strengthen clinical teacher training, improve teachers' ability, and continuously improve the clinical practice training model and the quality of talent training for nursing graduate students.