Objective:To explore the effect and mechanism of myricetin(Myr)on trinitrobenzenesulfonic acid(TNBS)-in-duced inflammatory bowel disease(IBD)in rats.Methods:IBD rat models were established and separated into Control group,Model group,low,medium and high dose Myr[28 mg/(kg·d),Myr-L,56 mg/(kg·d),Myr-M,112 mg/(kg·d),Myr-H]groups.The dis-ease activity index(DAI)of rats was scored.Immune function indicators and colon length were measured;reagent kits were applied to measure the levels of IL-6,IL-17A,TNF-α and cAMP in serum.MPO optical density values in colon tissue was detected.HE staining was applied to observe pathological changes in colon tissue.Flow cytometry was applied to determine the proportion of Treg cells.Results:Compared with the control group,the colon tissue cells in the model group were disordered in arrangement,with a large num-ber of inflammatory cell infiltrations,obvious ulcer phenomena,a large number of cell necrosis,mucosal edema and thickening of the colon wall,DAI score,IL-6,IL-17A and TNF-α levels,spleen index,thymus index and MPO optical density value was increased sig-nificantly(P<0.05),colon length,cAMP level and the proportion of Treg cells were significantly decreased(P<0.05).Compared with the model group,the colonic tissue cells in the low-dose,medium-dose and high-dose Myr groups of rats were arranged more neatly,cAMP membrane edema and colonic wall thickening were alleviated,and inflammatory cell infiltration,cell necrosis and ulceration phenomena were reduced,DAI score,levels of IL-6,IL-17A and TNF-α,spleen index,thymus index,and MPO optical density val-ue were gradually decreased(P<0.05),while the colon length,cAMP level,and the proportion of Treg cells were significantly in-creased(P<0.05).Conclusion:Myr may inhibit inflammation levels,regulate immune function,and exert protective effects on IBD rats.

Objective:To explore the effect and mechanism of myricetin(Myr)on trinitrobenzenesulfonic acid(TNBS)-in-duced inflammatory bowel disease(IBD)in rats.Methods:IBD rat models were established and separated into Control group,Model group,low,medium and high dose Myr[28 mg/(kg·d),Myr-L,56 mg/(kg·d),Myr-M,112 mg/(kg·d),Myr-H]groups.The dis-ease activity index(DAI)of rats was scored.Immune function indicators and colon length were measured;reagent kits were applied to measure the levels of IL-6,IL-17A,TNF-α and cAMP in serum.MPO optical density values in colon tissue was detected.HE staining was applied to observe pathological changes in colon tissue.Flow cytometry was applied to determine the proportion of Treg cells.Results:Compared with the control group,the colon tissue cells in the model group were disordered in arrangement,with a large num-ber of inflammatory cell infiltrations,obvious ulcer phenomena,a large number of cell necrosis,mucosal edema and thickening of the colon wall,DAI score,IL-6,IL-17A and TNF-α levels,spleen index,thymus index and MPO optical density value was increased sig-nificantly(P<0.05),colon length,cAMP level and the proportion of Treg cells were significantly decreased(P<0.05).Compared with the model group,the colonic tissue cells in the low-dose,medium-dose and high-dose Myr groups of rats were arranged more neatly,cAMP membrane edema and colonic wall thickening were alleviated,and inflammatory cell infiltration,cell necrosis and ulceration phenomena were reduced,DAI score,levels of IL-6,IL-17A and TNF-α,spleen index,thymus index,and MPO optical density val-ue were gradually decreased(P<0.05),while the colon length,cAMP level,and the proportion of Treg cells were significantly in-creased(P<0.05).Conclusion:Myr may inhibit inflammation levels,regulate immune function,and exert protective effects on IBD rats.

Objective: The current study aims to find out the potential reasons why most schizophrenia patients have a relatively low sensitivity to the classification of emotional facial expressions. Methods: By using an emotional categorical perception task, eighty-three schizophrenia patients and seventy-one healthy adults are provided with morphed emotional continuums with two emotional facial expressions (a positive emotional valence: happy; a negative emotional valence: sad). Results: Through comparing the difference between schizophrenia patients and healthy adults in the processes of estimating facial expressions with ambiguous emotions, we find that the pattern of emotional categorical perception for schizophrenia patients is significantly different from that of healthy controls when they process signals on the local facial areas. Compared to healthy people, schizophrenia patients have a significantly separate classification pattern in processing emotional signals between the eyes and mouth regions. It indicates that compared to healthy adults, schizophrenia patients have larger conflicts in integrating emotional signals from different facial areas. To overcome conflicts, more cognitive resources are required. Unfortunately, the lack of cognitive resources leads to the failure of integration, which further increases the difficulty of estimating facial expressions with ambiguous emotions, and finally leads to the relatively low sensitivity of emotional facial expressions classification. Conclusion To sum up, the deficit of abnormal perceptions of emotional facial expressions in schizophrenia patients results from an integrated deficit of signals on facial areas.

AIM:To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb from different areas in order to provide a base for the identification of its quality. METHODS: A gradient separated method was applied. Column:Inertsil ODS-3 C_ 18 ,mobile phase:acetonitrile-water,detection wavelength:270 nm,flow rate:1.0 mL/min,column temperature:25 ℃. RESULTS: To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb., make the technical parameters for its quality controlling,and mark 39 main peaks as its characteristic fingerprint. CONCLUSION: The distribution of constituents of Melia toosendan Sieb differ a little from different areas, but the propotion of the constituents differ greatly, with the Melia toosendan Sieb.from the same area , the distribution and propotion differ a little. This method is reproducible, simple and easy, and can be use to provide a base for the quality control of Melia toosendan Sieb.