Purpose To investigate the expression status of GNL3 in gastric cancer,and to explore the role of GNL3 in tumor proliferation,invasion and migration.Methods The ex-pression of GNL3 mRNA in gastric cancer tissues was analyzed by searching database.The expression of GNL3 protein in 51 gastric cancer tissues and 51 adjacent non-tumor tissues was de-tected by immunohistochemistry(IHC)SP method.The correla-tion between GNL3 protein expression and gastric cancer clinical pathological features was analyzed by x2 test.The expression of GNL3 in gastric cancer cells was silenced by transfection of sh-RNA,and the silencing efficiency was verified by Western blot.The effect of silencing GNL3 on the proliferation of gastric cancer cells was examined by CCK-8,colony formation and EdU stai-ning.In addition,wound-healing assay and Transwell assay were performed to detect the effect of GNL3 silencing on cell invasion and migration.Results SangerBox and UALCAN database re-trieval showed that the expression of GNL3 mRNA was signifi-cantly increased in gastric cancer tissues(P＜0.01).IHC stai-ning showed that the positive expression rate of GNL3 protein in gastric cancer tissues was 96.1％,and the high expression rate was 78.4％,which was significantly higher than that in adjacent non-tumor tissues(74.5％,51.0％,P＜0.01).Moreover,the high expression of GNL3 was significantly correlated with lymph node metastasis in gastric cancer patients(x2=4.933,P=0.026).CCK-8,colony formation and EdU staining showed that GNL3 silencing inhibited the proliferation of gastric cancer cell SGC-7901.The wound-healing and Transwell assay showed that GNL3 silencing inhibited the migration and invasion of gastric cancer cell.Conclusion The GNL3 protein is highly expressed in gastric cancer tissues,and closely related to the proliferation,migration and invasion of gastric cancer cells.

Objective:To analysis the necessity of additional surgical intervention for non-curative endoscopic resection patients with early gastric cancer.Methods:A retrospective analysis was conducted on 73 patients with early gastric cancer who underwent additional surgical procedures after non-curative endoscopic resection at Chinese PLA General Hospital from July 2009 to May 2023. The main outcome measures included pathological classification, positive horizontal margins, positive vertical margins, invasion depth, vascular and lymphatic invasion, eCura grade, lymph node metastasis, and overall survival rate.Results:A total of 73 patients with early gastric cancer who were determined to have non-curative endoscopic resection underwent additional surgical procedures, including 58 males and 15 females with a mean age of 61 (53-67) years. In terms of the site of onset, 37 cases were located in the upper part of the stomach, 24 cases in the lower part, 11 cases in the middle part, and 1 case had multiple lesions. In terms of pathological classification, 43 cases were highly differentiated tubular adenocarcinoma, 16 cases were mucinous/signet ring cell carcinoma, 10 cases were poorly differentiated tubular adenocarcinoma, and 4 cases were high-grade intraepithelial neoplasia. In terms of morphological classification, 22 cases were type 0-Ⅱa, 43 cases were type 0-Ⅱb, and 8 cases were type 0-Ⅲ. In terms of invasion depth, 17 cases were mucosal cancer, 23 cases had submucosal invasion less than 500 μm, and 33 cases had submucosal invasion more than 500 μm. In terms of vascular and lymphatic invasion, 8 cases had lymphatic vessel invasion and 8 cases had venous invasion. Among the 73 patients, 4 were diagnosed as having eCura A, 5 as eCuraB, 4 as eCura C1, and 60 as eCura C2. Among the 60 patients diagnosed as having eCura C2, only 2 cases (3.3%) were found to have lymph node metastasis around the stomach based on postoperative pathological evaluation. Among the 73 endoscopic specimens, 7 patients had positive horizontal margins, 21 had positive vertical margins, and 2 had positive margins in both directions, totaling 30 patients with positive horizontal or vertical margins. According to postoperative pathological evaluation, 9 cases (30.0%) had residual tumors in the original site. Among the 73 patients, 5 were lost to follow-up and 4 died, resulting in an overall survival rate of 94.12% (64/68) and disease-specific survival rate of 98.53% (67/68). The follow-up time of patients was 61.37 (10-166) months.Conclusion:For early gastric cancer patients with eCura C2 following non-curative endoscopic resection, additional surgery is feasible. However, the proportion of patients with actual lymph node metastasis is relatively low.

Objective:To analyze the management and treatment of patients with psychosis in china in 2020 and compare with those in the past five years to provide a reference for making policy and service delivery.Methods:Descriptive analysis was used to analyze the system usage and registration, management, and treatment situation of the patients with psychosis, using the data obtained from 'National Information System for Psychosis′ in China, with date traced from 1st August 2011 to 31st December 2020.Results:By the end of 2020, the information system was adopted by 100% of districts/counties. 6 430 587 patients were registered, with a registered rate of 0.46% (6 430 587/1 396 537 459). In 2020, 6 116 599 patients were provided with follow-up services by primary healthcare staff, with a management rate of 95.12% (6 116 599/6 430 587). 5 724 019 patients were followed up regularly, with the regular management rate of 89.01% (5 724 019/6 430 587). Moreover, the medication-taking rate was 87.83% (5 648 162/6 430 587), and the regular medication- taking rate was 68.84% (4 426 498/6 430 587). 5 277 746 patients were stable, and the stable condition rate was 96.40% (5 277 746/5 474 881). Compared with 2019, 200 430 more patients were registered in 2020. The management rate, regular management rate, medication-taking rate, regular medication-taking rate, and stable condition rate were increased by 0.88%, 2.50%, 3.75%, 11.39%, and 1.15%, respectively. The regular medication-taking rate among patients registered in the western region was significantly lower than that in the eastern and central areas ( F=6.317, P=0.005). According to the diagnosis, the duration of the untreated period of patients registered in 2020 with schizophrenia, bipolar disorder, delusional disorder, schizoaffective disorder, psychotic disorder with epilepsy, mental retardation with psychotic symptoms were 2.69, 3.28, 1.68, 1.92, 4.70, and 9.89 years respectively, which were shorter than patients registered in 2019. Conclusion:The number of registered patients was sustainably growing. The untreated periods of newly registered patients were considerably shorter. The services and treatment situations improved in 2020. Appropriate policies should be developed to address the weaknesses of local mental health services in the western region to improve treatment rates.

Objectives:To analyze the allocation of mental health resources in Chinese mainland and the change of these resources during the 13 th Five-year Mental Health Plan period. This analysis will serve as evidence for developing future national mental health prevention and control policies. Methods:The National Mental Health Program Office created one questionnaire to survey available mental health facilities by the end of 2020. Descriptive methods were used to analyze the mental health facilities, psychiatric beds, and mental health professionals from type, sponsor, administrative level, regions, and sub-specialities.Results:By the end of 2020, 5 936 facilities were providing mental health services in Chinese mainland. 798 191 psychiatric beds (5.65 beds/10 000 persons), 50 124 psychiatrists (3.55/100 000 persons), and 139 642 registered nurses (9.89/100 000 persons) were located in these facilities. Of all these 5 936 mental health facilities, 649 (1.09%) had geriatric psychiatric wards, 312 (0.53%) had child psychiatric wards, and 1 548 (26.08%) had rehabilitation departments. 350 counties (12.31%) had no mental health facilities and 883 counties (31.05%) had no psychiatric beds in Chinese mainland, mainly in the mid-western regions. The numbers of psychiatric beds, psychiatrists, and registered nurses per unit land area in the western region were about 4 times lower than those in the middle region and 7-11 times lower than those in the eastern region.Conclusions:The mental health resources in Chinese mainland increased significantly from 2015 to 2020, but there are still the problems of uneven geographical allocation of resources and lagged sub-specialities development. While formulating the construction and development plan for mental health prevention and control system in the future, we should further develop the mental health resources at the county level, and vigorously support to establish of mental health sub-specialities for the elderly and children, as well as mental health rehabilitation, particularly in the mid-western regions.

Objective:To analyze the management and treatment of patients with psychosis in china in 2020 and compare with those in the past five years to provide a reference for making policy and service delivery.Methods:Descriptive analysis was used to analyze the system usage and registration, management, and treatment situation of the patients with psychosis, using the data obtained from 'National Information System for Psychosis′ in China, with date traced from 1st August 2011 to 31st December 2020.Results:By the end of 2020, the information system was adopted by 100% of districts/counties. 6 430 587 patients were registered, with a registered rate of 0.46% (6 430 587/1 396 537 459). In 2020, 6 116 599 patients were provided with follow-up services by primary healthcare staff, with a management rate of 95.12% (6 116 599/6 430 587). 5 724 019 patients were followed up regularly, with the regular management rate of 89.01% (5 724 019/6 430 587). Moreover, the medication-taking rate was 87.83% (5 648 162/6 430 587), and the regular medication- taking rate was 68.84% (4 426 498/6 430 587). 5 277 746 patients were stable, and the stable condition rate was 96.40% (5 277 746/5 474 881). Compared with 2019, 200 430 more patients were registered in 2020. The management rate, regular management rate, medication-taking rate, regular medication-taking rate, and stable condition rate were increased by 0.88%, 2.50%, 3.75%, 11.39%, and 1.15%, respectively. The regular medication-taking rate among patients registered in the western region was significantly lower than that in the eastern and central areas ( F=6.317, P=0.005). According to the diagnosis, the duration of the untreated period of patients registered in 2020 with schizophrenia, bipolar disorder, delusional disorder, schizoaffective disorder, psychotic disorder with epilepsy, mental retardation with psychotic symptoms were 2.69, 3.28, 1.68, 1.92, 4.70, and 9.89 years respectively, which were shorter than patients registered in 2019. Conclusion:The number of registered patients was sustainably growing. The untreated periods of newly registered patients were considerably shorter. The services and treatment situations improved in 2020. Appropriate policies should be developed to address the weaknesses of local mental health services in the western region to improve treatment rates.

Objectives:To analyze the allocation of mental health resources in Chinese mainland and the change of these resources during the 13 th Five-year Mental Health Plan period. This analysis will serve as evidence for developing future national mental health prevention and control policies. Methods:The National Mental Health Program Office created one questionnaire to survey available mental health facilities by the end of 2020. Descriptive methods were used to analyze the mental health facilities, psychiatric beds, and mental health professionals from type, sponsor, administrative level, regions, and sub-specialities.Results:By the end of 2020, 5 936 facilities were providing mental health services in Chinese mainland. 798 191 psychiatric beds (5.65 beds/10 000 persons), 50 124 psychiatrists (3.55/100 000 persons), and 139 642 registered nurses (9.89/100 000 persons) were located in these facilities. Of all these 5 936 mental health facilities, 649 (1.09%) had geriatric psychiatric wards, 312 (0.53%) had child psychiatric wards, and 1 548 (26.08%) had rehabilitation departments. 350 counties (12.31%) had no mental health facilities and 883 counties (31.05%) had no psychiatric beds in Chinese mainland, mainly in the mid-western regions. The numbers of psychiatric beds, psychiatrists, and registered nurses per unit land area in the western region were about 4 times lower than those in the middle region and 7-11 times lower than those in the eastern region.Conclusions:The mental health resources in Chinese mainland increased significantly from 2015 to 2020, but there are still the problems of uneven geographical allocation of resources and lagged sub-specialities development. While formulating the construction and development plan for mental health prevention and control system in the future, we should further develop the mental health resources at the county level, and vigorously support to establish of mental health sub-specialities for the elderly and children, as well as mental health rehabilitation, particularly in the mid-western regions.