Objective To analyze the correlation between serum 25-hydroxyvitamin D[25(OH)D]and inflammatory cytokines such as interferon γ(IFN-γ),C-reactive protein(CRP)and interleukin-2(IL-2)in neonatal pneumonia.Methods A total of 100 neonates admitted to First Hospital of Qinhuangdao from December 2018 to December 2020 were enrolled in this study.According to the serum 25(OH)D level,they were divided into deficiency group(≤15.0 μg/L,n=18),inadequate group(15.1-20 μg/L,n=42),and adequate group(＞20.0 μg/L,n=40).The general and clinical data such as gender,gestational age,serum 25(OH)D level,birth weight,mode of delivery and the levels of IFN-γ,CRP and IL-2 were compared among the three groups.The correlation between vitamin D deficiency and levels of IFN-γ,CRP and IL-2 was analyzed.Results There were no significant differences in gestational age,sex,birth weight,or delivery mode among the three groups(P＞0.05).Serum 25(OH)D levels in the three groups were as follows:deficiency group＜inadequate group＜adequate group,and there were significant differences in the serum 25(OH)D level between groups(P＜0.05).The levels of IFN-γ,CRP and IL-2 in the deficiency group were higher than those in the inadequate group and adequate group(P＜0.05),and the levels of IFN-γ,CRP and IL-2 in the inadequate group were higher than those in the adequate group(P＜0.05).The Pearson correlation analysis showed that serum 25(OH)D level was negatively correlated with IFN-γ,CRP and IL-2 levels in newborns(P＜0.05).Conclusion The lower the serum 25(OH)D in newborns,the more deficient the vitamin D and the higher the levels of IFN-γ,CRP and IL-2 are,indicating higher risk of pneumonia.

Objective:To investigate the expression of phosphorylated glutamate receptor 2[p-GluR2(S880)]in oligodendrocyte precursor cells(OPCs)of mice model of hypoxic ischemic brain injury(HIBI).Methods:The HIBI model of C57BL/6 neonatal mice was established by right common carotid artery ligation and hypoxia for 90 min.The anxiety-like behavior of the mice was evaluated by elevated plus maze(EPM)and open field test(OFT).The expres-sion of p-GluR2(S880),oligodendrocyte marker 4(O4)and myelin basic protein(MBP)in brain tissue of HIBI model mice was detected by immunofluorescence staining.What's more,the expression levels of p-GluR2(S880)and MBP were detected by Western Blot.Results:Compared with sham operation group,there were significant anxiety-like behaviors 90 days after HIBI operation(P＜0.05).The expression of MBP protein decreased significantly in 14 and 28 days after HIBI operation.The expression of p-GluR2(S880)protein was up-regulated at all time points after HIBI op-eration(P＜0.05),and the number of O4 and p-GluR2(S880)double positive cells in brain tissue of HIBI group was significantly increased(P＜0.05).Conclusion:The up-regulation of p-GluR2(S880)expression in OPCs may lead to myelination disorder in HIBI model mice.

Objective To study the effect of coenzymes complex combined with vitamin E on liver and kidney inju-ry induced by neonatal hyperbilirubinemia. Methods One hundred and fifty full-term neonatals with hyperbilirubinemia were chosen as observation groups, who were divided into mild, moderate and severe groups according bilirubin. Forty five healthy full-term newborns in the same period, who are either healthy or with physiological jaundice, were selected as con-trol group. Serum total bilirubin, gamma-glutamyl transferase (γ-GGT) , Malondialdehyde (MDA) and Cys-C were measured within 24 hours of hospital admission.The observation groups were randomly divided into regular and combined treatment groups. Coenzymes complex combined with vitamin E were given in addition to regular method to combined group while reg-ular group only received regular methods.All above biochemical indexes were tested in the 7th day after medication admin-istration. Results Serum MDA were higher in all observation groups (mild, moderate and severe groups) than in control group (P＜0.05);but the levels ofγ-GGT and Cys-C increased in moderate and severe groups compared with control group (P＜0.05). There were positive correlations (P＜0.05) between levels of serum total bilirubin with MDA,γ-GGT and Cys-C. Positive linear correlation were found between MDA with γ-GGT and Cys-C(P＜0.01). After early intervention,γ-GGT, Cys-C and MDA declined with drop of bilirubin level. This is more prominent and faster in a in combined treatment group than regular group (P＜0.05).Conclusion In hyperbilirubinemia newborns, lipid peroxidation activated by bilirubin may lead to damages of liver and kidney. Coenzymes combined with vitamin E have protective effect to these damages.

AIM:To investigate the effect of exogenous epidermal growth factor(EGF) on expression of EGFR and EGF mRNA of the injuried lung tissue of neonatal premature rats exposed to hyperoxia.METHODS:The neonatal Sprague-Dawley rats of 21-day gestational age model of hyperoxia induced lung injury were made by continually inhaling 95% oxygen.The model rats were divided into two groups randomly,the EGF trail group and the NS control group.The other rats were taken into the air control group.Each group was divided into three subsets:a(1-3 days),b(4-6 days) and c(1-6 days) according to different application times of EGF or NS.Rats in sub groups were executed and the lung tissues were removed at postnatal 3th,7th,14th day respectively.Using immunohistochemistry method,the expression of EGF-R of lung tissues in different groups was observed,and the expression of EGF mRNA was determined by reverse transcriptase polymerase chain reaction(RT-PCR).RESULTS:The expression of EGF mRNA increased by degrees following the increasing postnatal days.Compared with the air control group,the expression of EGF-R and EGF mRNA increased in the hyperxia group at 7th day and 14th day.The expression of EGF-R increased in corresponding hyperxia NS control group at 14th day(P