Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

Objective:To evaluate the clinical efficacy and safety of Pazopanib in maintenance therapy after platinum-resistant recurrence of ovarian cancer.Methods:A total of 80 patients with platinum-resistant recurrence of ovarian cancer treated in Sichuan Friendship Hospital from January 2018 to January 2024 were selected as the study objects.All patients were given paclitaxel or gemcitabine monotherapy for 6-8 cycles and entered mainte-nance therapy after the patients achieved complete response(CR)or partial response(PR).They were divided into a study group(38 cases)and a control group(42 cases)using a simple randomization method.The study group was given pazopanib maintenance treatment,and the control group was given compound cantharides cap-sule maintenance treatment.The treatment time lasted until the disease progressed.Progression-free survival(PFS),serum CA125 level,1-year survival rate,and adverse drug reactions were compared between the two groups.Results:Compared with the control group,PFS(12.84±6.23 months vs.6.12±4.82 months)and 1-year survival rate(73.68%vs.45.24%)in the study group were higher than those in the control group,the difference was statistically significant(P<0.05).The serum CA125 level of the study group was lower than that of the control group after 3 months of maintenance treatment and when the tumor recurred again,and the difference was statis-tically significant(P<0.05).The overall incidence of adverse drug reactions in the study group was higher than that in the control group(52.63%vs.21.42%,P<0.05),but mainly chronic diarrhea,hair loss and hypopigmenta-tion.Conclusions:In the maintenance treatment after recurrence of platinum-resistant patients with ovarian canc-er,oral pazopanib can effectively prolong the disease progression-free period of patients with few side effects,high safety,and strong patient compliance.

Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

Objective:To evaluate the clinical efficacy and safety of Pazopanib in maintenance therapy after platinum-resistant recurrence of ovarian cancer.Methods:A total of 80 patients with platinum-resistant recurrence of ovarian cancer treated in Sichuan Friendship Hospital from January 2018 to January 2024 were selected as the study objects.All patients were given paclitaxel or gemcitabine monotherapy for 6-8 cycles and entered mainte-nance therapy after the patients achieved complete response(CR)or partial response(PR).They were divided into a study group(38 cases)and a control group(42 cases)using a simple randomization method.The study group was given pazopanib maintenance treatment,and the control group was given compound cantharides cap-sule maintenance treatment.The treatment time lasted until the disease progressed.Progression-free survival(PFS),serum CA125 level,1-year survival rate,and adverse drug reactions were compared between the two groups.Results:Compared with the control group,PFS(12.84±6.23 months vs.6.12±4.82 months)and 1-year survival rate(73.68%vs.45.24%)in the study group were higher than those in the control group,the difference was statistically significant(P<0.05).The serum CA125 level of the study group was lower than that of the control group after 3 months of maintenance treatment and when the tumor recurred again,and the difference was statis-tically significant(P<0.05).The overall incidence of adverse drug reactions in the study group was higher than that in the control group(52.63%vs.21.42%,P<0.05),but mainly chronic diarrhea,hair loss and hypopigmenta-tion.Conclusions:In the maintenance treatment after recurrence of platinum-resistant patients with ovarian canc-er,oral pazopanib can effectively prolong the disease progression-free period of patients with few side effects,high safety,and strong patient compliance.

Objective:To investigate the clinical application value of qualitative and quantitative analysis of Sonazoid contrast-enhanced ultrasound(CEUS)in the 4 category of breast lesions of the Breast Imaging Reporting and Data System(BI-RADS)of diagnosis.Methods:A total of 53 patients with breast lesions(All 53 lesions were BI-RADS 4 category)who underwent biopsy or surgery in Chengdu First People's Hospital from April 2022 to April 2023 were selected.According to the pathological results of breast nodules,they were respectively divided into benign and malignant groups.All patients had underwent Sonazoid CEUS before biopsy or surgery.The differences of the qualitative and quantitative indexes of the breast nodules CEUS between benign and malignant groups were compared.The indexes with statistical significances were fitted into regression equation.And then,the receiver operating characteristic(ROC)curves were drawn for obtaining area under curve(AUC).The differences of enhanced strengths of breast nodules and surrounding normal tissues in CEUS were coded to intuitive color images,and generated dynamic vascular model(DVP)parameter picture by using Sonoliver software.The differences of image colors between benign and malignant breast nodules were analyzed.Results:According to the results of biopsy or surgical pathology,the 53 breast nodules were respectively divided into benign group(34 cases)and malignant group(19 cases).In qualitative indexes of Sonazoid-CEUS for breast nodules,the most appearance of the malignant nodules showed 13 cases were the enlargement of enhancement range(68.4%),and 14 cases were uneven enhancement(73.7%)and 12 cases were peripheral perforating blood flow(63.2%),and the differences of them between benign and malignant group were statistically significant(x2=10.268,9.642,5.717,P＜0.05),respectively.In the quantitative index of Sonazoid-CEUS for breast nodules,the rise time(RT)and mean transition time(mTT)of breast nodules of malignant group were significantly shorter than those of benign group,and the differences were statistically significant(Z=-1.985,-2.272,P＜0.05).After the above indexes were fitted into multiple factors analysis,the regression equations of predicting malignant nodules by using qualitative and quantitative indexes were respectively established.The AUC values of ROC curves of qualitative and quantitative analyses were respectively 0.723 and 0.902.The result of qualitative analysis was significantly better than that of quantitative analysis(Z=-1.965,P＜0.05).In DVP parametric images,the nodules of 23 cases(67.6%)of 32 nodules of benign group appeared lesions,and the most in region of interesting(ROI)appeared monotonous red/yellow filling,and 15 cases(78.9%)of 19 cases of malignant group appeared lesions,and the ROI inside appeared patchy red/yellow filling of disordered,irregular,scattered or clumpy shape.Conclusion:Sonazoid CEUS can discriminate benign and malignant nodules of breast BI-RADS 4 category,and the diagnostic efficacy of qualitative indexes is better than that of quantitative indexes..

With the continuous development of experimental biology, the limitations of commonly utilized model organisms are becoming increasingly apparent. Discrepancies between research conducted on laboratory animals and humans significantly impede the translational application of findings derived from animal experiments. This review introduces ascidian Ciona intestinalis as a novel model organism, an invertebrate that is evolutionarily closest to vertebrates and is a sister group to vertebrates. The review summarizes recent research progress on Ciona intestinalis in various fields to illustrate the significant advantages and promising application prospects of it as a model organism. The research progress outlined in the review mainly encompasses: (1) The whole-genome sequencing of Ciona intestinalis has been determined and numerous related databases have been established. Various embryonic gene editing technologies have been successfully applied, making it an animal model easy to manipulate genetically and study the functions and interactions of target genes visually. (2) In the field of neurobiology, Ciona intestinalis boasts a central nervous system structure similar to that of vertebrates and possesses numerous homologous neuropeptides and hormone molecules. These features grant it an edge in exploring the mechanisms and functional evolution of endocrine and neuroendocrine-related molecules. Additionally, the sensitivity and habituation of its larvae to light stimulation provide an avenue for exploring mechanisms related to behavioral plasticity. (3) In the field of immunology, Ciona intestinalis possesses a mature innate immune system and has evolved precursor genes to the adaptive immune system, with a relatively simple coding of immune-related genes. These features make it an exemplary model organism for immunological studies. (4) In the field of developmental biology, many studies have focused on the notochord development process in Ciona intestinalis and the regulatory mechanisms of gene expression within it, indicating common evolutionary developmental strategies among chordates. Additionally, insights into its heart development also significantly enhance our comprehension on the genetic network of human heart development. (5) In medical research, the ability of Ciona intestinalis to regenerate its neural complex and siphon, as well as the resilience of its heart to recover contractile function from substantial damage, renders it a valuable animal model for the study of regeneration and heart injury. It also has unique advantages as a research model for Alzheimer's disease and new drug development. Furthermore, its brief five-month lifespan facilitates the observation and recording of the entire aging process and the exploration of the effects of various factors on aging. In summary, this review aims to demonstrate that Ciona intestinalis stands out as a model organism with unique attributes and is expected to play a significant role in a wider range of scientific research areas.

Objective:To investigate the efficacy of levodopa and benserazide hydrochloride combined with pramipexole in the treatment of Parkinson's disease in 30 patients and their effects on neurotransmitters and oxidative stress response.Methods:A total of 90 patients with Parkinson's disease admitted to Jinhua People's Hospital from January 2020 to February 2022 were included in this study. They were randomly assigned to undergo treatment with levodopa and benserazide hydrochloride (levodopa and benserazide hydrochloride group), pramipexole (pramipexole group), or their combination (combined therapy group), with 30 patients in each group. All patients were treated for 12 consecutive weeks. Clinical efficacy, levels of brain neurotransmitters (dopamine, 5-hydroxytryptamine, norepinephrine, and substance P), and oxidative stress response (superoxide dismutase, malondialdehyde, homocysteine levels) were compared among the three groups.Results:Total response rate in the combined therapy group was 96.67% (29/30), which was significantly higher than 66.67% (20/30) in the levodopa and benserazide hydrochloride group and 76.67% (23/30) in the pramipexole group ( χ2 = 8.65, P < 0.05). After treatment, dopamine, 5-hydroxytryptamine, norepinephrine, substance P, superoxide dismutase, malondialdehyde, and homocysteine levels in the combined therapy group were (9.05 ± 1.24) ng/mg, (89.49 ± 10.69) μg/L, (15.16 ± 1.36) ng/mg, (102.8 ± 15.36) μg/L, (88.40 ± 10.04) kU/L, (5.5 ± 2.31) μmol/L, and (9.20 ± 3.36) μmol/L, respectively, which were superior to (6.61 ± 1.02) ng/mg, (68.52 ± 9.52) μg/L, (12.33 ± 1.24) ng/mg, (151.64 ± 16.03) μg/L, (74.99 ± 7.28) kU/L, (9.27 ± 3.07) μmol/L, and (13.52 ± 3.64) μmol/L in the levodopa and benserazide hydrochloride group and (7.22 ± 1.09) ng/mg, (79.52 ± 10.20) μg/L, (13.92 ± 1.31) ng/mg, (131.30 ± 15.65) μg/L, (80.59 ± 8.24) kU/L, (7.53 ± 2.93) μmol/L, (11.35 ± 3.71) μmol/L in the pramipexole group ( F = 38.53, 32.05, 35.49, -73.42, 18.42, -22.65, -12.13, all P < 0.05). Conclusion:Levodopa and benserazide hydrochloride combined with pramipexole are highly effective on Parkinson's disease. The combined therapy can effectively improve brain neurotransmitters and regulate oxidative stress response.

Objective: A model was built by neural network analysis to study the relationship between different degrees of vitamin B12 and folic acid deficiency and malnutrition-induced stomatitis. Methods: Data from 30 healthy volunteers and 30 patients with malnutrition-induced stomatitis were collected. The distribution of lesions, the number of affected sites and clinical manifestations were recorded, and the severity was scored. The levels of vitamin B12 and folic acid in the peripheral blood of the two groups were simultaneously measured. The SPSS software was used to analyze the correlation between vitamin B12 and folic acid levels in the peripheral blood of patients with malnutrition-induced stomatitis and healthy volunteers, and the MATLAB software package was used to analyze the data via a neural network. Results: The levels of vitamin B12 and folic acid significantly correlated with the grade of malnutrition-induced stomatitis. Simultaneous B12 and folic acid deficiency linearly correlated with the occurrence and severity of malnutrition-induced stomatitis. Based on this correlation, a thermogram model of malnutrition-induced stomatitis was constructed. Conclusion Malnutrition-induced stomatitis is closely related to vitamin B12 and folic acid deficiency. Their synergistic effect may promote the occurrence and development of malnutrition-induced stomatitis. The construction of the malnutrition-induced stomatitis model aids the targeted etiological treatment of patients with moderate and severe deficiency to prevent malnutrition-induced stomatitis.

Objective To study the importance of radial height restoration in the type C distal radial fractures and selection of therapeutic method.Methods Sixty-one cases of type C distal radial fractures in the department of joint orthopedics and traumatic orthopedics of affiliated traumatic and orthopedic hospital of Guangzhou university of Chinese medicine from June 2013 to September 2015 were retrospectively analyzed, including 34 cases of C1 type,14 cases of C2 type and 13 cases of C3 type according to the AO/ASIF fracture classification, the cases were divided into the manual reduction and splint external fixation group (27 cases), external fixator group (18 cases) and plate screw internal fixation group (16 cases).The radial height, palm inclination angle, ulnar inclination angle and Gartland-Werley (G-W) score after treatment were compared among various groups.Results Fifty-eight cases were followed up.The radial height, ulnar inclination angle and G-W score had statistical difference among 3 groups (P<0.05), while the palm inclination angle had no statistical difference among 3 groups (P>0.05);the difference of radial height and ulnar inclination angle between the external fixator group and plate screw internal fixation group had no statistical significance (P>0.05), but there were statistically significant differences when these two groups were compared with the manual reduction and splint external fixation group respectively (P<0.05).The G-W score had no statistical difference between the manual reduction splint fixation group and the external fixator group (P>0.05) , but there were statistically significant differences when these two groups were compared with the the plate screw internal fixation group respectively (P<0.05).Conclusion The radial height restoration may better recovery ulnar inclination angle and improve the joint function.

BACKGROUND:Distal radius C fractures belong to multiple fractures in the joint. Its treatment should recover various angles and joint surface continuity outside the joint. The recovery of palmar tilt angle, radial inclination angle and radial height is also very important for treating distal radius. OBJECTIVE:To study the changes in palmar tilt angle, radial inclination angle and radial height after type C fractures of the distal radius repaired with Acu-Loc(R)2 VDR bone plate system. METHODS:From May 2015 to March 2016, 11 cases of type C distal radial fractures were treated with Acu-Loc(R)2 VDR bone plate system. Preoperatively, the palmar tilt angle was-31° to-4° (-12.45° on average), and the radial inclination angle was 6° to 18° (11.18° on average), and the radial height was 1.92 mm to 8.68 mm (5.28 mm on average). X-ray films were observed at postoperative 4, 8 and 12 weeks and half a year in all patients. Palmar tilt angle, radial inclination angle and radial height were measured and observed using anteroposterior and lateral X-ray films during final follow-up. Wrist function was assessed with Gartland-Werley score. RESULTS AND CONCLUSION:(1) 11 patients were followed and the postoperative follow-up ranged from 7 months to 15 months. X-ray films showed that the union of fractures was achieved in 6-8 weeks (6.5 weeks on average). No infection or internal fixation failure occurred. The articular facets were smooth in 10 patients and a little poor was found in 1 case (<2 mm). (2) After operation, the palmar tilt angle was 8° to 15° (11.55° on average) (P<0.05);the radial inclination angle was 22° to 27° (23.18° on average) (P<0.05);the radial height was 8.01 mm to 13 mm (11.03 mm on average) (P<0.05). (3) The results were excellent in 8 cases, good in 2 cases, and poor in 1 cases according to Gartland-Werley wrist function assessment;the excellent and good rate was 91%. (4) Acu-Loc(R)2 VDR bone plate fixation can provide reliable fixation and effective support for type C distal radius fractures, which can recover the palmar tilt angle, the radial inclination angle and the radial height, so the Acu-Loc(R)2 VDR bone plate is an ideal method to treat type C fractures of the distal radius.