Objective:To explore the differences in the infertility stress, subjective well-being and fertility quality of life among infertile couples and mediating role of psychological resilience.Methods:A cross-sectional study and convenient sampling method were used to recruit the new diagnosed as infertility couples from April 2017 to April 2018 in the Reproductive Center of the First Affiliated Hospital of Zhengzhou University. The Resilience Scale (CD-RISC), the Fertility Problem Inventory (FPI), the Subjective Well-being Scale (SWBS) and the Fertility Quality of Life Questionnaire (FertiQoL) were used to investigate the 230 infertile couples.Results:Infertility couples had statistical differences in psychological resilience ( P=0.034), infertility stress ( P<0.001), and fertility quality of life ( P=0.005), and there was no statistically significant difference in subjective well-being. The fitting degree of structural equation model of infertility stress and subjective well-being and fertility quality of life among infertility women was relatively ideal [χ 2/ df=1.437, goodness of fit index (GIF)=0.936, adjusted goodness of fit index (AGFI)=0.909, root mean square error of approximation (RMSEA)=0.043], and the mediating effects of psychological resilience on subjective well-being and fertility quality of life was significant. The fitting degree of structural equation model of infertility stress and subjective well-being and fertility quality of life among infertility men was relatively ideal [χ 2/ df=2.011, GFI=0.93, incremental fit index (IFI)=0.918, comparative fit index (CFI)=0.917, RMSEA=0.067], and psychological resilience had a mediating effect on fertility quality of life, but had no mediating effect on subjective well-being. Conclusion:There are differences in infertility stress and fertility quality of life among infertile couples. psychological resilience has a mediating effect on the infertility stress and fertility quality of life among infertile couples.

Objective:To explore the relationship between salivary alpha-amylase (SAA) and infertility stress in infertile women.Methods:A convenient sampling method was used to investigate the infertile women in the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from April 2017 to December 2017. A total of 458 infertile women were examined using the SAA and the Fertility Problem Inventory (FPI). SAA level was measured using an enzyme-linked immunosorbent assay (ELISA) kit.Results:The total infertility stress score was 155.86±24.54. The family monthly income, the cause of infertility and the type of infertility were statistically different between different groups ( t=4.123, P=0.041; F=4.079, P<0.001; t=4.491, P=0.035). Pearson correlation analysis showed that SAA and social concern, relationship concern, need for parenthood, rejection of child-free life, sexual concern and total infertility stress were significantly correlated ( r=0.360, r=0.348, r=0.222, r=0.166, r=0.203, r=0.426, all P<0.001). Multiple linear regression results showed that infertility stress, infertility causes and types of infertility were factors influencing SAA. Conclusion:SAA was associated with infertility stress. Combined with SAA and FPI may assess the psychological stress more objectively for infertile women.

Objective:To explore the differences in the infertility stress, subjective well-being and fertility quality of life among infertile couples and mediating role of psychological resilience.Methods:A cross-sectional study and convenient sampling method were used to recruit the new diagnosed as infertility couples from April 2017 to April 2018 in the Reproductive Center of the First Affiliated Hospital of Zhengzhou University. The Resilience Scale (CD-RISC), the Fertility Problem Inventory (FPI), the Subjective Well-being Scale (SWBS) and the Fertility Quality of Life Questionnaire (FertiQoL) were used to investigate the 230 infertile couples.Results:Infertility couples had statistical differences in psychological resilience ( P=0.034), infertility stress ( P<0.001), and fertility quality of life ( P=0.005), and there was no statistically significant difference in subjective well-being. The fitting degree of structural equation model of infertility stress and subjective well-being and fertility quality of life among infertility women was relatively ideal [χ 2/ df=1.437, goodness of fit index (GIF)=0.936, adjusted goodness of fit index (AGFI)=0.909, root mean square error of approximation (RMSEA)=0.043], and the mediating effects of psychological resilience on subjective well-being and fertility quality of life was significant. The fitting degree of structural equation model of infertility stress and subjective well-being and fertility quality of life among infertility men was relatively ideal [χ 2/ df=2.011, GFI=0.93, incremental fit index (IFI)=0.918, comparative fit index (CFI)=0.917, RMSEA=0.067], and psychological resilience had a mediating effect on fertility quality of life, but had no mediating effect on subjective well-being. Conclusion:There are differences in infertility stress and fertility quality of life among infertile couples. psychological resilience has a mediating effect on the infertility stress and fertility quality of life among infertile couples.

Objective:To explore the relationship between salivary alpha-amylase (SAA) and infertility stress in infertile women.Methods:A convenient sampling method was used to investigate the infertile women in the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from April 2017 to December 2017. A total of 458 infertile women were examined using the SAA and the Fertility Problem Inventory (FPI). SAA level was measured using an enzyme-linked immunosorbent assay (ELISA) kit.Results:The total infertility stress score was 155.86±24.54. The family monthly income, the cause of infertility and the type of infertility were statistically different between different groups ( t=4.123, P=0.041; F=4.079, P<0.001; t=4.491, P=0.035). Pearson correlation analysis showed that SAA and social concern, relationship concern, need for parenthood, rejection of child-free life, sexual concern and total infertility stress were significantly correlated ( r=0.360, r=0.348, r=0.222, r=0.166, r=0.203, r=0.426, all P<0.001). Multiple linear regression results showed that infertility stress, infertility causes and types of infertility were factors influencing SAA. Conclusion:SAA was associated with infertility stress. Combined with SAA and FPI may assess the psychological stress more objectively for infertile women.

Oncolytic viruses have made great breakthroughs in cancer treatment, especially for reovirus, which can effectively induce the death of tumor cells without harming the normal tissues. More than 80％ tumor cells are sensitive to reovirus infection. Reovirus induces the apoptosis of tumor cells and exerts anti-tumor immunity to achieve anti-tumor activity, and the curative effect of combination therapy with reovirus and chemotherapeutic drugs exceeds the effect of monotherapy. Reovirus can exert anti-tumor effects through different mechanisms, which is of great significance for the new and effective treatment of tumors in the future.

Objective To realize the distribution and characteristics of Mycobacterium leprae (M.leprae) species and its single nucleotide polymorphisms (SNPs) spreading currently in China.Methods A total of 171 cutaneous lesion specimen of leprosy patients from 22 provinces were collected.The 16S rRNA conservative region of Mycobacterium leprae was amplified by nest PCR and the positive products were sequenced directly and aligned by BLAST.The SNPs of M.leprae were genotyped by restricted fragment length polymorphism for the PCR products.Results The 171 specimen were all Mycobacterium leprae since the amplified fragments of DNA samples were 99％ similar to the Br4923 of M.leprae from Brazil.No new species (M.lepromatosis) was found.Among the 85 samples genotyped for SNPs,SNP3,SNP1 and SNP2 accounted for 78.8％ (67/85),20％ (17/85) and 1.2％ (1/85) respectively.There was no sample with SNP4 genotype to be detected.Among the 171 sequencing specimen,130 showed mutation C-T at 251 bp of 16S rRNA.There was no difference for mutant rate of 16S rRNA gene and SNP genotype among the samples with different clinical pathological types.Certain associations between 16S rRNA C251T mutation and SNP genotype were found.Most of the samples with C251T mutations of 16S rRNA sequence were SNP3,only a few were SNP1 but not SNP2.There was significant difference of SNP genotype distribution among the patients from different regions.The distribution rate of SNP3 genotype in the samples from inland region (97.1％,34/35)was significantly higher than that from coastal region (66％,33/50) (x2 =11.96,P ＜ 0.01) . There was significant difference of the gene mutation rate of 16S rRNA sequence among the patients from different regions.The mutation rate of 16S rRNA in the samples from inland region (94.8％,92/97) was significantly higher than that from coastal region (51.4％,38/74) (x2 =43.56,P ＜0.01).Conclusion C251T mutation in 16S rRNA gene sequence of M.leprae may associate with SNP type suggesting that the characteristics of geographical distribution presented in different genotypes of M.leprae.No new species of M.leprae was found in this study.

Objective To report the treatment outcome of nonunion of tibial fractures with local muscle flap transfer and injection of autogenous bone marrow. Methods 15 patients who had suffered from nonunion of tibial fracture 10 to 42 (mean 22) months after the initial injury underwent open reduction, internal fixation with locking intramedullary nail, wound coverage with locally transferred muscle flap and injection of autogenous bone marrow into the fracture site 12 to 15 days after operation. Results The follow-ups revealed bone union in all the cases of this series with a mean healing time of 22 (3 to 11) months. Except for limited necrosis of the skin edge in 2 cases which healed after dressing changes, the wounds healed primarily in all the cases without infection and implant failure. Conclusion Besides stable internal fixation and bone graft, coverage of fracture site with locally transferred muscle flap and injection of autogenous bone marrow can be used to treat nonunion of tibial fractures with satisfactory results.

Objective To detect genetic mutation of apolipoprotein B-100(apoB-100)in patients with primary hypercholesterolemia.Methods One special segment of apoB-100 gene from nucleotide 10549 to 10895 was amplified by polymerase chain reaction(PCR).The PCR products were denatured and hybridized with specific aligonucleotide labeled with digoxigenin,and were analyzed by single strand conformation polymorphism(SSCP)to detect the apoB-100 gene mutation 3531CGC→CGT or other mutations.Results Overall 41 members of 11 primary hypercholesterolemia families were detected,but the above genetic mutation was not detected.Conclusion This genetic mutation is unlikely to exist or of significantly low incidence in Chinese population,and might not be the main cause of primary hypercholesterolemia in the 11 primary hypercholesterolemia families.