Objective To evaluate the efficacy of elastic appliances in the early correction of Class Ⅱmalocclusions in the replacement dentition.Methods A total of 15 children aged 7-9 years who were admit-ted to the Affiliated Stomatology Hospital of Southwest Medical University from June 2018 to August 2023 were selected as the observation group,and 15 children who were consulted but not treated in the hospital dur-ing the same period were selected as the control group.The changes of bone tissue,dental and alveolar tissue,soft tissue and occlusal relationship before and after treatment were evaluated in the two groups.In addition,X-ray images of the observation group were collected every 8 to 10 months for the whole process dynamic mo-nitoring.Results Before treatment,there was no significant difference in all indexes between the two groups(P＞0.05).After treatment,there were statistical significances in the upper and lower alveolar base Angle(ANB),mandibular branch length(GO-CO),mandibular height(ANS-Me),mandibular position(S-GO),up-per central incisor inclination(U1-NA Angle),upper central incisor inclination convexity(U1-NA),lower central incisor process distance(L1-APo),the Z Angle and FH-N'Pg'Angle of soft tissue between the two groups(P＜0.05).After treatment,the covering OB and covering OJ in the observation group were lower than those in the control group,and the proportion of ClassⅠ patients in molar relationship was higher than that in the control group,with statistical significance(P＜0.05).After 3-4 years of treatment,ANB gradual-ly decreased,and the anterior-basilar plane-mandibular plane Angle(SN-MP)remained basically stable,and had a slight decreasing trend in the later period.The U1-NA Angle and the lower central incisor inclination(L1-NB Angle)were close to the normal mean during the whole treatment.Conclusion Using elastic appli-ances to treat patients with early replacement Class Ⅱ malocclusion,after 3-4 years monitoring and guid-ance,it can effectively improve the molar relationship,promote forward jaw growth,and create a harmonious and aesthetically pleasing facial soft tissue.

Objective:To compare the clinical effect of surgical treatment of congenital preauricular fistulas in children during the local infection period and static inflammatory period. Methods:Forty children with congenital preauricular fistula infection treated in our hospital from January 2020 to December 2022 were selected as the experimental group, and 39 children with congenital preauricular fistula inflammation at static period were selected as the control group. The fistula of the two groups of children aged between 1-14 years old was located in front of the foot of the ear wheel or the foot of the ear wheel, and all were unilateral fistulas. The postoperative follow-up was 6 months to 2 years, and the efficacy of the two groups was compared. Results:There was no significant difference in the healing rate of stage Ⅰ and stage Ⅱ between the two groups（P>0.05）. There was no significant difference in fistula recurrence rate and satisfaction with the preauricular scar between the two groups after treatment（P>0.05）. There was no significant difference in postoperative hospital stay between the experimental group and the control group（P>0.05）. Conclusion:The effect of surgical treatment of congenital preauricular fistula in the infected period is similar to that of surgical treatment in the static period of inflammation, and it can reduce the pain of dressing change under local anesthesia in children, avoid the second operation in children, and reduce the economic cost. This treatment method is worthy of clinical promotion. Appropriate incision and resection method were designed according to the fistula and infection sites.
Humans ; Child ; Infant ; Child, Preschool ; Adolescent ; Fistula/surgery* ; Inflammation ; Craniofacial Abnormalities/surgery* ; Cicatrix ; Treatment Outcome

Objective:To investigate the role of miRNA-4298/PADI4/p53 signal axis in mediating 4f-induced apoptosis of leukemia cells.Methods:The cell growth density was observed under inverted microscope and the proliferation of leukemia cells was detected by CCK-8 counting assay. The expression of PADI4 and P53 at mRNA level was detected by qRT-PCR. Cell cycle and apoptosis were measured with flow cytometry. The expression of PADI4, P53, Bcl-2, Bax, caspase-3 and caspase-9 at protein level was detected by Western blot. Differential miRNA and mRNA expression profiles was detected by next generation sequencing. Databases such as TargetScan were used to predict the potential upstream and downstream genes of PADI4. A luciferase reporter assay was used to detect the 3′UTR of PADI4 targeted by miRNA-4298. Cell transfection assay was used to detect the effect siRNA, PADI4 vector, miRNA mimics and miRNA inhibitor in interference and rescue.Results:Nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor 4f could inhibit the proliferation of THP-1, K562 and U937 cells, and induce the apoptosis of these leukemia cells. It downregulated the expression of PADI4 mainly through the binding activity of miRNA-4298 to miRNA sponges, which resulted in the proliferation inhibition and apoptosis of leukemia cells. The inhibited proliferation and apoptosis of leukemia cells by 4f were associated with the increase of P53 expression after the decrease of PADI4 expression. The PADI4-dependent upregulation of P53 led to the ratio inversion of downstream Bcl-2/Bax, which activated caspase-3 or caspase-9 to induce the apoptosis of leukemia cells.Conclusions:The apoptosis of leukemia cells induced by Nrf2 inhibitor 4f was mainly associated with the miRNA-4298/PADI4/p53 axis, suggesting that it might be a novel signaling pathway for targeted therapy.

Objective:To preliminarily evaluate the myocardium of pilots by noninvasive and radiation free cardiac MRI mapping technology, and to explore its application value in evaluating the myocardium of pilots.Methods:Case-control study was adopted in this study. Pilots and normal personnel who had cardiac MRI in Air Force Medical Center from October of 2020 to July of 2022 were selected, and they were divided into 20-40 years group and >40 years group. The differences of myocardial longitudinal relaxation time T1, transverse relaxation time T2 and T2 * values between pilots and normal personnel at different ages were analyzed and compared. Pilots were divided into groups according to flying hours and aircraft types, and the differences of myocardial T1, T2 and T2 * values among pilots in different flying hours and aircraft types were analyzed and compared. Results:The average myocardial T1 value of pilots [(1 011.18±24.70) ms] was lower than that of normal personnel [(1 025.07±25.74) ms], and the difference was significant ( t=2.10, P=0.040). The myocardial T1 value of pilots >40 years old [(1 006.04±29.15)ms] was lower than that of normal personnel [(1 029.68±20.24) ms], and the difference was significant ( t=2.30, P=0.032). The myocardial T1 value of fighter pilots [(999.04±17.39)ms] was lower than that of the bomber, transporter, helicopter pilots [(1 023.33±25.53) ms], and the difference was significant ( t=2.73, P=0.012). There was no significant difference in myocardial T1, T2 and T2 * values between pilots and nornal personnel aged 20-40 years old; there was no significant difference in T2 and T2 * values between pilots and normal personnel in the age group of >40 years old (all P>0.05). There was also no significant difference in myocardial T1, T2 and T2 * values among the pilots in different flying hours (all P>0.05). Conclusions:The level of cardiomyocyte hypertrophy or myocardial fibrosis in fighter pilots might be lower than that in other aircraft types pilots. Cardiac MRI mapping has potential value in evaluating the myocardium of pilots.

Objective:To analyze the correlation between serum vitamin D level and ovarian artery blood flow index and ovarian reserve function in women.Methods:A retrospective cohort study was conducted. Women aged from 18 to 40 years who were admitted to the Department of Obstetrics and Gynecology of the Second Affiliated Hospital of Harbin Medical University from September 2020 to November 2021 and had regular sexual life were selected as subjects. The data of age, height and weight were collected. And the indexes of vitamin D, anti-Müllerian hormone (AMH), luteinizing hormone and follicle-stimulating hormone were detected in the laboratory. Color Doppler ultrasound was used to measure antral follicle count and bilateral ovarian interstitial artery blood stream resistance index (RI) and plusatility index (PI) in early follicular phase. Multiple linear regression was used to analyze the related factors of serum vitamin D level.Results:A total of 218 women were included in the study, including 137 cases of vitamin D deficiency, accounting for 62.8%, 39 cases of vitamin D insufficiency, accounting for 17.9%, 42 cases of normal vitamin D, accounting for 19.3%. Multiple linear regression analysis showed that there was a negative correlation between serum vitamin D level and ovarian blood flow ( P=0.024), but no correlation with other indexes (all P>0.05). Conclusion:Serum vitamin D level affected ovarian interstitial artery blood flow RI to some extent, but had no significant correlation with ovarian interstitial artery blood flow PI, serum AMH level and other indexes related to ovarian reserve function.

Objective:To analyze the correlation between serum vitamin D level and ovarian artery blood flow index and ovarian reserve function in women.Methods:A retrospective cohort study was conducted. Women aged from 18 to 40 years who were admitted to the Department of Obstetrics and Gynecology of the Second Affiliated Hospital of Harbin Medical University from September 2020 to November 2021 and had regular sexual life were selected as subjects. The data of age, height and weight were collected. And the indexes of vitamin D, anti-Müllerian hormone (AMH), luteinizing hormone and follicle-stimulating hormone were detected in the laboratory. Color Doppler ultrasound was used to measure antral follicle count and bilateral ovarian interstitial artery blood stream resistance index (RI) and plusatility index (PI) in early follicular phase. Multiple linear regression was used to analyze the related factors of serum vitamin D level.Results:A total of 218 women were included in the study, including 137 cases of vitamin D deficiency, accounting for 62.8%, 39 cases of vitamin D insufficiency, accounting for 17.9%, 42 cases of normal vitamin D, accounting for 19.3%. Multiple linear regression analysis showed that there was a negative correlation between serum vitamin D level and ovarian blood flow ( P=0.024), but no correlation with other indexes (all P>0.05). Conclusion:Serum vitamin D level affected ovarian interstitial artery blood flow RI to some extent, but had no significant correlation with ovarian interstitial artery blood flow PI, serum AMH level and other indexes related to ovarian reserve function.

Objective:To preliminarily evaluate the myocardium of pilots by noninvasive and radiation free cardiac MRI mapping technology, and to explore its application value in evaluating the myocardium of pilots.Methods:Case-control study was adopted in this study. Pilots and normal personnel who had cardiac MRI in Air Force Medical Center from October of 2020 to July of 2022 were selected, and they were divided into 20-40 years group and >40 years group. The differences of myocardial longitudinal relaxation time T1, transverse relaxation time T2 and T2 * values between pilots and normal personnel at different ages were analyzed and compared. Pilots were divided into groups according to flying hours and aircraft types, and the differences of myocardial T1, T2 and T2 * values among pilots in different flying hours and aircraft types were analyzed and compared. Results:The average myocardial T1 value of pilots [(1 011.18±24.70) ms] was lower than that of normal personnel [(1 025.07±25.74) ms], and the difference was significant ( t=2.10, P=0.040). The myocardial T1 value of pilots >40 years old [(1 006.04±29.15)ms] was lower than that of normal personnel [(1 029.68±20.24) ms], and the difference was significant ( t=2.30, P=0.032). The myocardial T1 value of fighter pilots [(999.04±17.39)ms] was lower than that of the bomber, transporter, helicopter pilots [(1 023.33±25.53) ms], and the difference was significant ( t=2.73, P=0.012). There was no significant difference in myocardial T1, T2 and T2 * values between pilots and nornal personnel aged 20-40 years old; there was no significant difference in T2 and T2 * values between pilots and normal personnel in the age group of >40 years old (all P>0.05). There was also no significant difference in myocardial T1, T2 and T2 * values among the pilots in different flying hours (all P>0.05). Conclusions:The level of cardiomyocyte hypertrophy or myocardial fibrosis in fighter pilots might be lower than that in other aircraft types pilots. Cardiac MRI mapping has potential value in evaluating the myocardium of pilots.

Objective:To study the value of ultrasound-guided endoscopic retrograde appendicitis therapy in children with uncomplicated appendicitis.Methods:This study was a single center, retrospective study, including all electronic cases of appendicitis diagnosed clinically in Department of Pediatrics, the Second Affiliated Hospital of Air Force Military Medical University from October 2018 to October 2020 and received ultrasound-guided endoscopic retrograde appendicitis therapy.The clinical features, treatment and prognosis of the children were retrospectively analyzed.Results:A total of 152 electronic cases were included, there were 77 males and 75 females, aged(6.84±3.09) years.All the 152 children were treated with ultrasound-guided endoscopic retrograde appendicitis therapy.Intubation success rate and clinical success rate was 98.03%(149/152 cases)and 97.99%(146/149 cases), respectively.The median time of endoscopic therapy was 42.50 (31.00, 56.00) minutes.Mean postoperative hospital stay was (2.81 ±1.41) days, and the mean total hospital stay was (4.19 ±1.71) days.A total of 139 patients were followed up with a median follow-up time of 5 (1, 26) months.During the follow-up, the recurrence rate was 7.19%(10/139 cases), and the median time of recurrence was 2 (1, 3) months.Conclusions:Ultrasound-guided endoscopic retrograde appendicitis therapy had high effective rate and low recurrence rate in children with uncomplicated appendicitis, preserved the physiological function of appendix and avoided radiation damage.It can be used as a safe and effective treatment for acute and chronic uncomplicated appendicitis in children.

Objective: To investigate the difference in intestinal microbiome between children with atopic dermatitis (AD) and healthy children. Methods: Totally, 35 children with AD were enrolled from the Department of Dermatology, Jiading Hospital of Traditional Chinese Medicine from April 2015 to April 2017, and 27 healthy children served as control group. Total DNA was extracted from the feces of the subjects, and the V3-V4 region of the 16S rRNA gene of the bacteria was amplified by PCR. High-throughput sequencing was performed using the Illumina Miseq sequencing platform to analyze the diversity of bacterial flora. The top 15 abundant bacteria were determined at phylum, genus, and species levels, and compared between the two groups. Statistical analysis was carried out using Wilcoxon rank sum test. Results: The intestinal microbiome in the two groups mainly consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. At the phylum level, the abundance of Bacteroidetes and Fusobacteria was significantly lower in the AD group (29.16% ± 19.96%, 0.06% ± 0.17%, respectively) than in the healthy control group (39.06% ± 15.98%, 0.50% ± 1.71%, respectively, P = 0.042, 0.041) . At the genus level, the abundance of Bacteroides was significantly lower in the AD group (23.77% ± 18.08%) than in the healthy control group (33.1% ± 15.75%, P = 0.029) . There was no significant difference in the distribution of the top 15 abundant species between the two groups (all P > 0.05) . Conclusion There are some differences in the composition of intestinal microbiome and relative abundance of bacteria between children with AD and healthy children.

Objective To establish a molecular method for the identification of different serotypes of group B streptococcus(GBS)based on TaqMan fluorescence probe technology,and to lay the foundation for the sub-sequent study of multiple fluorescent probe technology to detect different serotypes of GBS.Methods Primers and probes were designed according to the different serotypes of capsular polysaccharide(CPS).CPS se-quences were amplified by real-time fluorescence quantitative polymerase chain reaction.GBS classification methods of different serotypes were established.The results were compared with latex agglutination test and the method was evaluated from the aspects of sensitivity,specificity and detection of clinical isolates.Results The logarithmic concentration of DNA in the same serotype GBS was linearly correlated with the value of Ct. The detection limit of this method is 1 pg/μL,a probe could only detect the corresponding serotype GBS.The results of TaqMan fluorescence probe test of 10 strains were consistent with the results of latex agglutination test.Conclusion TaqMan fluorescence probe technique is a simple,rapid,highly sensitive and specific method for the detection of different GBS serotypes,and it is better than latex agglutination test for the classification of clinical isolates.