Objective: To explore the association of physical activity and sugar sweetened beverage consumption with psychological sub health among middle school students in Bao an District, Shenzhen, so as to provide a reference for adolescent mental health promotion. Methods: A questionnaire survey was conducted in November 2024 by a stratified cluster random sampling method to select 6 926 junior and senior middle school students from 5 middle schools in Shenzhen. The questionnaire from Youth Risk Behavior Surveillance System was used to assess the consumption of sugar sweetened beverages, and physical activity Rating Scale was used to assess the level of physical activity, and Brief Instrument on Psychological Health of Youths was used to evaluate the psychological sub health status. The Chi -square test was used to analyze the differences in the detection rates of psychological sub health among different groups of middle school students, and a multivariate Logistic regression model was established to analyze the effects of physical activity and sugar sweetened beverage consumption and their combined effects on the psychological sub health of middle school students. Results: The detection rate of psychological sub health among middle school students in Bao an District, Shenzhen was 18.93%. Multivariate Logistic regression analysis showed that, after controlling for confounding factors such as gender, school stage, family residence, family economic status, parental literacy, academic stress and number of friends, lack of physical activity or excessive sugar sweetened beverage consumption were associated with increased risks of psychological sub health among middle school students ( OR =1.36, 1.45); and the highest risk of psychological sub health was found in middle school students who were lack of physical activity and excessive sugar sweetened beverage consumption ( OR =2.59) ( P <0.01). Further analysis by school stages showed that junior high school students with sufficient physical activity and excessive intake of sugary drinks ( ROR =2.10), lack of physical activity and excessive intake of sugary drinks ( ROR =2.31) were at higher risks of psychological sub health than senior high school students( P <0.05). Conclusions Insufficient physical activity and excessive sugar sweetened beverage consumption are closely associated with an increased risk of psychological sub health among middle school students. Effective interventions should be targeted to reduce the risk of psychological sub health problems among middle school students.

Objective: To investigate the assocation of sedentary behavior among college students on psychological health issues, such as depressive, anxiety, and stress symptoms, and to analyze the moderating role of takeaway consumption behavior in the context, in order to provide a scientific basis for reducing emotional symptoms among college students. Methods: A stratified cluster sampling method was employed to conduct a questionnaire on 3 427 first year students of a higher education institution in Hefei of Anhui Province from May to June 2021. The study variables included demographic characteristics, sedentary time, takeaway consumption behavior, and emotional (symptoms depressive, anxiety, and stress symptoms). The Spearman correlation analysis was used to analyze the association between variables, and linear regression analysis was used to analyze the association between takeaway consumption behavior and depressive, anxiety and stress symptoms among college students with sedentary time. Results: Both sedentary time and takeaway consumption behavior were positively correlated with depressive, anxiety and stress symptoms among college students ( r =0.10, 0.10, 0.10; 0.10, 0.11, 0.11, all P <0.05). The results of linear regression analysis showed that the interaction term between takeaway consumption behavior and sedentary time was positively correlated with symptoms of depressive, anxiety, and stress symptoms among college students (depression: β =0.04, anxiety: β =0.04, stress: β =0.04, all P <0.05). The results of the simple slope test demonstrated that regardless of the level of takeaway consumption behavior, sedentary time was positively correlated with the depressive symptoms of college students; compared with low takeaway consumption behavior, high takeaway consumption behavior ( β=0.77, P <0.01) enhanced the association between sedentary time and depressive symptoms among college students. In addition, under the condition of high takeaway consumption behavior, sedentary time was positively correlated with the anxiety and stress symptoms of college students (anxiety: β =0.64; stress: β =0.71, both P <0.01); while under the condition of low takeaway consumption behavior, sedentary time was not related to the anxiety and stress symptoms of college students ( β =0.17, 0.22, both P >0.05). Conclusions Sedentary behavior is related to a the emotional symptoms of depressive, anxiety, and stress among college students. Takeaway consumption behavior may exacerbate this impact.

Objective: To analyze the correlation and gender differences between adverse childhood experiences (ACEs) and positive childhood experiences (PCEs) and depression and anxiety symptoms among middle school students, so as to provide a reference for promoting the mental health of middle school students. Methods: With a stratified random cluster sampling method, a total of 6 656 middle school students in 4 cities, including Nanchang, Shenyang, Taiyuan, and Zhengzhou, were selected as research subjects from October 2021 to October 2022. The Adverse Childhood Experiences International Questionnaire (ACEs-IQ), Benevolent Childhood Experiences Scale (BCEs), Patient Health Questionnaire-9 (PHQ-9) and Generalized Anxiety Disorders-7 (GAD-7) scale were used to conduct questionnaire surveys.The Chi square test was used to compare the reporting rates of depression and anxiety symptoms among middle school students in different groups, and a Logistic regression model was established to analyze the effects of ACEs and PCEs on depression and anxiety symptoms among middle school students and their gender differences. Results: The reporting rate of depressive symptoms among middle school students was 20.1%, and the reporting rate of anxiety symptoms was 13.9% . ACEs were positively correlated with depression and anxiety symptoms among middle school students (depression symptoms: OR =1.20, 95% CI =1.18-1.22, anxiety symptoms: OR =1.18, 95% CI =1.16-1.20), while PCEs were negatively correlated with depression and anxiety symptoms among middle school students(depression symptoms: OR =0.84, 95% CI = 0.83 -0.86, anxiety symptoms: OR =0.85, 95% CI =0.83-0.87) ( P <0.05). In the general population (depression symptoms ： OR =0.99, 95% CI = 0.98- 0.99, anxiety symptoms: OR =0.99, 95% CI =0.99-1.00) and among girls (depression symptoms: OR = 0.98 , 95% CI = 0.97- 0.99 , anxiety symptoms ： OR =0.99, 95% CI =0.98-1.00), the interaction term between ACEs and PCEs were negatively correlated with depression and anxiety symptoms ( P <0.05). Conclusions ACEs significantly affect the depression and anxiety symptoms of middle school students, while PCEs can help reduce the impact of ACEs on the depression and anxiety symptoms of middle school students, girls are more susceptible to the impact of early experiences than boys. It should focus on gender differences, formulate comprehensive mental health protection strategies, to promote the mental health development of middle school students.

New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design. Here, we identified a receptor-binding domain (RBD)-binding antibody, XG014, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its circulating variants, SARS-CoV and bat SARSr-CoV WIV1. Interestingly, antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibody-dependent SARS-CoV-2 spike (S) protein-mediated cell-cell fusion, suggesting a unique mode of recognition by XG014. Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional "down" conformation, while its family member XG005 directly competes with ACE2 binding and position the RBD "up". Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo. Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines against β-CoV-B and newly emerging SARS-CoV-2 variants of concern.
Angiotensin-Converting Enzyme 2 ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Epitopes ; Humans ; SARS-CoV-2/genetics* ; Spike Glycoprotein, Coronavirus/genetics*

ObjectiveTo investigate the prevenance rate and related factors of anxiety among the public before and after outbreak of COVID-19， and to provide scientific guidance for public health emergency response in psychological intervention field. MethodsBy using the convenient sampling method， residents in Chinese mainland participated in predesigned questionnaire survey from February 7^th to February 14^th， 2020， meantime， all the selected individuals were assessed using Self-rating Anxiety Scale （SAS）. The anxiety status was also retrospectively evaluated before January 20^th， prior to the outbreak. Thereafter， a before-and-after comparison was conducted on the anxiety status， and the related influencing factors were discussed. ResultsA total of 1 222 valid questionnaires were collected， with a valid rate of 93.8%. After the outbreak， the number of people with anxiety symptoms increased from 90 （7.4%） to 172 （14.1%）， with statistical difference （P<0.01）. The SAS score increased from （40.65±10.43） to （36.32±8.46）， with statistical difference （P<0.01）. For overall sample， the independent risk factors of anxiety aggravation included serious disruptions of daily life （P<0.01）， older age （P<0.01）， female （P<0.01）， poor education background （P=0.005）， occupied in medical staff （P=0.031） and lack of medical education （P=0.039）. For medical staff， the independent risk factors of anxiety aggravation included sense of being-alienated （P<0.01）， older age （P<0.01）， female （P=0.002） and serious disruptions of daily life （P=0.044）. ConclusionThe prevalence rate of anxiety is increased after the outbreak of COVID-19， especially among the general public with serious disruptions of daily life， older age， females， poor education background， and the lack of medical education， and among medical staff with sense of being-alienated， older age， females， and serious disruptions of daily life.

Objective:To explore the relationship between executive dysfunction and brain functional connectivity in deaf children and its mechanisms.Methods:Twenty-eight deaf children were recruited from special education schools and twenty-seven children with normal hearing were treated as controls matched with deaf children in age, sex and education. All human subjects were scheduled to complete brain magnetic resonance scanning, based on the voxel-based morphometry (VBM) technique for detecting differences in gray matter volume (GMV) and a set of executive function (EF) tasks for detecting differences in EF behavior performance between deaf and normal hearing children. All human subjects were also performed resting-state fMRI, which were analyzed by independent component analysis (ICA) and the executive control network was selected and compared between the deaf and normal hearing children.Results:Compared with the children with normal hearing, deaf children had smaller volume of gray matter in the left angular gyrus (MIN coordinates: -48, -72, 45; 700 voxels), right superior parietal gyrus (MIN coordinates: 20, -62, 57; 594 voxels) and left middle occipital gyrus(MIN coordinates: -32, -87, 42; 782 voxels). Deaf children had weaker functional connectivity (FC) in the left middle frontal gyrus, orbital part/left inferior frontal gyrus (MIN coordinates: -24, 21, 3; 54 voxels) with normal hearing children. In the behavioral test, deaf children had defects in the switching function compared with normal children, which was statistically significant ( t(42)=2.39, P=0.02). Conclusion:The EF of deaf children has some defects, and its neural mechanism may be related with the reduced functional connectivity and gray matter volume in the brain regions related to executive function.

Objective:To comprehensively and systematically measure and analyze deaf children's gray matter cortex and white matter fibers by surface-based morphometry (SBM) and tract-based spatial statistics(TBSS).Methods:Twenty-seven deaf children aged 9-13 years old and twenty-seven age and sex matched normal controls were selected. T1 structural images and diffusion tensor imaging data were collected and analyzed by SBM and TBSS to calculate the cortical thickness, back index and anisotropic index (fractional anisotropy, FA). The SPSS 20.0 software and FSL software were used for data analysis.Results:Compared with the control group, the thickness of the cortex in the left cerebral hemisphere central posterior gyrus, the superior lobule, the central lateral lobule, and the anterior lobe were significantly reduced(cluster size 4 150, P<0.05), and in the right cerebral hemisphere squats and the middle sacral region reduced(cluster size 2 592, P<0.05). The local regression index of the left anterior wedge region was significantly increased(cluster size 3 225, P<0.05). The DTI results showed a decrease in FA values in the areas of radiation crown, cortical bundle, cingulate gyrus, corpus callosum, thalamus radiation, and sub-occipital bundle( P<0.05, TFCE corrected). Conclusion:There are different degrees of damage in the cerebral cortex and white matter microstructure of deaf children, and the brain structure remodeling and compensatory reconstruction appeared in the anterior wedge, which provide strong evidence for in-depth study of relationship between the loss of auditory function and changes in the brain structure.

OBJECTIVE: To evaluate the application of femtosecond laser technology in the management of subluxated lens. METHODS: We retrospectively analyzed the data of the patients with subluxated lens undergoing femtosecond laser- assisted surgery at the Cataract Center of Guangzhou Aier Eye Hospital between March, 2017 and May, 2019. The LenSx femtosecond laser-assisted cataract surgery system was used to perform capsulotomy and lens fragmentation. According to the patients' eye condition, anterior vitrectomy was performed and capsular retractors was used. After phacoemulsification, I/A and insertion of the tension rings, the intraocular lens (IOL) was implanted into the capsular bag. The perioperative data, complications, visual acuity and intraocular pressure after the operation were recorded, and the stability of the capsular bag and IOLs were assessed. RESULTS: We analyzed the data of 25 cases (29 eyes) of subluxated lens, including 16 (16 eyes; 55.17%) as the result of traumatic lens subluxation, 5 (9 eyes; 31.03%) of Mafan syndromes, 1 case (1 eye; 3.45%) of high myopia and 3 cases (3 eyes; 10.34%) of unknown causes. Thirteen 13 eyes (44.83%) showed mild subluxation, 7 (24.14%) had moderate subluxation, and 9 (31.03%) had severe subluxation. Femtosecond laser- assisted capsulorhexis, lens fragmentation and phacoemulsification were successfully completed for 29 eyes, of which 28 eyes (96.55%) retained the complete capsular bag and with successful implantation of the capsular tension devices and IOLs. Nine eyes (31.03%) were treated with anterior segment vitrectomy; iris hooks were used for 2 eyes (6.90%) and capsular bag hooks for 9 eyes (31.03%). The best corrected visual acuity was significantly improved in 29 eyes after operation ( < 0.05). At 1 month after the surgery, 26 eyes (89.66%) showed stably centered IOLs, 2 eyes (6.90%) showed slight tilt of the IOLs, and 3 eyes (10.34%) had anterior capsular contraction. The intraoperative complications included subconjunctival hemorrhage (75.87%), incomplete capsulotomy (17.24%) and contracted pupils (13.79%). CONCLUSIONS The application of femtosecond laser assisted technology enhances the surgical safety and effectiveness for subluxated lens, facilitates the choice of individualized surgical options, and promotes maximum recovery of the patients' visual function.
Humans ; Laser Therapy ; Lens Implantation, Intraocular ; Lens Subluxation ; surgery ; Lenses, Intraocular ; Phacoemulsification ; Retrospective Studies

Objective To summarize the nursing experience of a patient with secondary catheter complications caused by cerebrospinal fluid drainage after endovascular aorticrepair. Methods A retrospective analysis was made on the clinical situation of a patient who received cerebrospinal fluid drainage afterendovascular aorticrepair in 2017, and the cause of secondary catheter complications, symptoms and signs were identified and nursing. Results After careful observation, mean arterial pressure maintenance, painmanagement, cerebrospinal fluid drainage velocity management, catheter infection risk and control, psychological intervention and exercise rehabilitation, the patient was finally recovered and discharged. Conclusions In order to evaluate the postoperative complications, we should not only focus on the surgery but also on the adverse events caused by cerebrospinal fluid drainage. In addition,we should improve the ability to identify the source of problems, to risk management and to disease assessment.

ObjectiveTo evaluate the application effect of phased intensive medication education (PIME) on patients after percutaneous coronary intervention(PCI). MethodsBy convenience sampling method, 60 patients who underwent PCI for the first time in Department of Cardiology, Second Affiliated Hospital of Shantou University Medical College from October 2017 to February 2018 were selected and randomly divided into experimental group (n=30) and control group (n=30). The experimental group was educated by PIME, while the control group received conventional medication education. The patients were followed up after discharge. Seven indicators, including medication literacy, medication compliance and exercise tolerance and so on were collected and compared. ResultsThere was no significant difference in the scores of medication literacy between the two groups before intervention (P＞ 0.05). On the day of discharge, 1 month and 3 months after discharge, the scores of the experimental group were higher than those of the control group, and the differences were statistically significant (P＜0.01). There was no significant difference in the scores of compliance between the two groups before intervention (P＞0.05). At 2 weeks, 1 month and 3 months after discharge, the scores in the experimental group were higher than those in the control group with statistical differences (P＜0.01). There was no significant difference in exercise tolerance between the two groups before intervention, at discharge and 1 month after discharge (P＞0.05). The experimental group was higher than the control group at 3 months after discharge with a statistical difference (P＜0.01). The decrease of heart rate and systolic blood pressure in the experimental group was greater than that in the control group (P＜ 0.05). One month after discharge, the incidence of adverse medication reactions in the experimental group was lower than that in the control group and the difference was statistically significant (P＜ 0.05). Conclusions PIME can effectively improve patients' medication literacy, medication adherence, safety and effectiveness, which is worthy of promotion.